Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | CP000730 | 400,404 | A→G | 9.7% | S13P (TCA→CCA) | tatC ← | Tat family twin arginine targeting transporter TatC |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | CP000730 | 400,404 | 0 | A | G | 9.7% | 104.6 / 3.0 | 41 | S13P (TCA→CCA) | tatC | Tat family twin arginine targeting transporter TatC |
Reads supporting (aligned to +/- strand): ref base A (18/19); new base G (2/2); total (20/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 |
ATGAAACAAAACGCGATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCTGTTGTTTCGATTCCTTACTGGGTGTA > CP000730/400274‑400541 | atgaaaCAAAACGCGATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATacg < 2:155436/141‑1 (MQ=255) atgaaaCAAAACGCGATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATacg < 1:16844/141‑1 (MQ=255) aaCAAAACGCGATGAAAAATATAATCATCCCGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATAcgacga < 1:384396/141‑1 (MQ=255) aaCAAAACGCGATGAAAAAGATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATAcgacga < 2:456035/141‑1 (MQ=255) aaaaCGCGATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCAAGGATAATATACATTATGAATGGTGTCATCAACCAAAATGATGAAACATATACGACGATGa < 2:304923/141‑1 (MQ=255) aaCGCGATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGAcc > 2:178823/1‑141 (MQ=255) aaCGCGATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGAGGAAACATATACGACGATTAcc > 1:315504/1‑141 (MQ=255) gATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGGAACATATACGACGATGACCGTAAt > 1:336494/1‑141 (MQ=255) gATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGGAACATATACGACGATGACCGTAAt > 1:100166/1‑141 (MQ=255) aTGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATg > 1:424068/1‑141 (MQ=255) aaTCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACaa < 2:387138/141‑1 (MQ=255) aaTCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAACGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACaa > 1:182092/1‑141 (MQ=255) tcatcaCGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATa < 1:352952/141‑1 (MQ=255) cGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAAttt > 1:338607/1‑141 (MQ=255) tttGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTa > 2:135667/1‑141 (MQ=255) tttGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTa > 1:311554/1‑141 (MQ=255) gTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAAcc < 2:316089/141‑1 (MQ=255) aTTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATgg < 1:14589/141‑1 (MQ=255) tgtgAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAg > 1:145508/1‑141 (MQ=255) tgtgAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAg < 1:252364/141‑1 (MQ=255) tgaatgaaAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATTAAACATGTACGACGATGACCGTAATGACGAACGACAATAAGATTTCTACTAACCGATGGCGTAGtt < 2:318914/141‑1 (MQ=255) atgaaAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGc > 1:61561/1‑141 (MQ=255) atgaaAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAAAGACAATAAAAATTTTACTAACCGATAGCGTAGTTCGc > 2:87739/1‑141 (MQ=255) cATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGGAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGaa < 2:336494/141‑1 (MQ=255) cATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGGAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGaa < 2:100166/141‑1 (MQ=255) aTGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAAc < 2:403154/141‑1 (MQ=255) aTGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAAc < 2:171384/141‑1 (MQ=255) tAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACTGTGACGACGAACGACAATAGTATTTTAACTAAACGATGACGTAGCTCGCTAAAATGAActag < 1:12508/141‑4 (MQ=255) cacaTGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGgt > 1:37280/1‑141 (MQ=255) caTGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGgtgt > 2:398434/1‑141 (MQ=255) tgtgCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTgg > 2:230851/1‑141 (MQ=255) gtgCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACTGTGACGACGAACGACAATAGTATTTTAACTAAACGATGACGTAGCTCGCTAAAATGAACTAGTAAGGTGTGGt > 1:2890/1‑141 (MQ=255) gtgCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGt > 2:322484/1‑141 (MQ=255) gCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCa < 1:108898/141‑1 (MQ=255) aCGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGt < 1:169103/141‑1 (MQ=255) tAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTAt > 1:6331/1‑141 (MQ=255) aaTGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCtgttg > 1:225987/1‑141 (MQ=255) aaTGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCtgttg < 2:389298/141‑1 (MQ=255) aTCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCTGTTGTTTCGATTc < 2:315413/141‑1 (MQ=255) aTCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCTGTTGTTTCGATTc > 1:120801/1‑141 (MQ=255) tCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCTGTTGTTTCGATTcc < 1:357668/141‑1 (MQ=255) atgaAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCTGTTGTTTCGATTCCTTACTGGGTGTa > 1:160138/1‑141 (MQ=255) | ATGAAACAAAACGCGATGAAAAATATAATCATCACGTATATTTGTATCATTTCTGTGAATGAAAATGCATGTAAGGACACATGTGCACGGGTAATATACGTTATGAATGGTGTCATCCACCAAAATGATGAAACATATACGACGATGACCGTAATGACGAACGACAATAAAATTTTTACTAACCGATGGCGTAGTTCGCTAAAGTGAACCAGTAAGGTGTGGTCAGTGCTATTGCTCTCGCTGTTGTTTCGATTCCTTACTGGGTGTA > CP000730/400274‑400541 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |