Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	124,550	G→A	10.9%	D229N (GAT→AAT)	norG →	possible bifunctional GntR family transcriptional regulator/aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	124,550	0	G	A	10.9%	116.3 / 5.7	46	D229N (GAT→AAT)	norG	possible bifunctional GntR family transcriptional regulator/aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (23/18);  new base A (2/3);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

CAACTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACATAATAT  >  CP000730/124412‑124686                                                                                                                                           |                                                                                                                                         cAAATTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGAt                                                                                                                                        <  2:209792/141‑1 (MQ=255)   aCTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATAc                                                                                                                                      >  1:375578/1‑141 (MQ=255)    cTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATAcc                                                                                                                                     <  2:266842/141‑1 (MQ=255)           cTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTg                                                                                                                              >  2:396600/1‑141 (MQ=255)                    ttttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATtta                                                                                                                     <  1:206273/141‑1 (MQ=255)                     tttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttat                                                                                                                    >  1:92335/1‑141 (MQ=255)                      ttAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATttatt                                                                                                                   <  1:292310/141‑1 (MQ=255)                        aGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTaa                                                                                                                 >  2:439507/1‑141 (MQ=255)                         ggTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAAt                                                                                                                >  2:311285/1‑141 (MQ=255)                                   ccATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATa                                                                                                      >  1:296485/1‑141 (MQ=255)                                           atTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGAtt                                                                                              >  2:176372/1‑141 (MQ=255)                                                  aTACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAg                                                                                       <  1:190331/141‑1 (MQ=255)                                                         atcatATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAg                                                                                >  1:154237/1‑141 (MQ=255)                                                               tatTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaa                                                                          >  2:298089/1‑141 (MQ=255)                                                                 ttCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaata                                                                        >  1:59045/1‑141 (MQ=255)                                                                 ttCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaata                                                                        >  2:177937/1‑141 (MQ=255)                                                                 ttCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTACAAACAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTtaata                                                                        <  1:87211/141‑1 (MQ=255)                                                                 ttCACTCTACAAATGTTTTTGAGAAATTGGACTTTTGTCATATTTATGTTCCTTAGAATCAAATTAATGAGATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTAATATAGAACATATGTTgaata                                                                        >  2:128910/1‑141 (MQ=255)                                                                       ctACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGa                                                                  >  2:83608/1‑141 (MQ=255)                                                                           aaaTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAgg                                                              >  1:74578/1‑141 (MQ=255)                                                                           aaaTGGTGTTGAGCAATATAATTTTACACATATTGATGTTCCTTATAATCAAATTAATGAACTAGATACCAGCATCGATAGATTTATTAATTTTAAAAATAAAGCGCTTTAGATAGAACCTAGGTTTAATAACCCGACAgg                                                              >  2:224818/1‑141 (MQ=255)                                                                             aTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTc                                                            <  2:292455/141‑1 (MQ=255)                                                                             aTGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTc                                                            >  2:296135/1‑141 (MQ=255)                                                                              tGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCg                                                           <  1:353731/141‑1 (MQ=255)                                                                              tGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCg                                                           <  1:207556/141‑1 (MQ=255)                                                                                 ttttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTc                                                        <  1:208643/141‑1 (MQ=255)                                                                                 ttttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTc                                                        <  1:128060/141‑1 (MQ=255)                                                                                  tttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCt                                                       <  1:15302/141‑1 (MQ=255)                                                                                  tttGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCCATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCt                                                       <  1:215220/141‑1 (MQ=255)                                                                                    tGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCttt                                                     <  1:152526/141‑1 (MQ=255)                                                                                     gAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTAATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTa                                                    <  1:167924/141‑1 (MQ=255)                                                                                          aTTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGaa                                               >  1:12774/1‑141 (MQ=255)                                                                                           ttGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAAt                                              >  2:25106/1‑141 (MQ=255)                                                                                               aTTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGc                                          <  1:454767/141‑1 (MQ=255)                                                                                               aTTTTAGACATATTGATGTTCCTTATAATAAAATTAATGAAATTGATACCATAAAAAAAAAAAAAAAAAAATTTAAAAATAAAGCGATTTATATACCACCTAGGTGTAATAACCCGACAGGGCGTTCTTTAACGaaaaaaa                                          <  2:233381/141‑6 (MQ=255)                                                                                                ttttAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTCATTTTACAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCa                                         <  1:409867/141‑1 (MQ=255)                                                                                                    aGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAg                                     >  1:203171/1‑141 (MQ=255)                                                                                                        atatTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGaaaa                                 <  1:269011/141‑1 (MQ=255)                                                                                                         tatTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGaaaaa                                <  1:83608/141‑1 (MQ=255)                                                                                                          ttttaTGTTCCTTAAAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCTATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAat                               >  2:394182/5‑141 (MQ=255)                                                                                                                        taataaAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATGAAGCGATTTATATAGAACCTCGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGAAAAAGAAAAATATAATTACTTATAg                 <  2:224600/141‑1 (MQ=255)                                                                                                                          aTCAAATTATTTTAATTGATACGATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCg               >  2:91223/1‑141 (MQ=255)                                                                                                                                     gAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACataat    >  1:328453/1‑141 (MQ=255)                                                                                                                                      aaaTTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATCGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACataata   >  1:8043/1‑141 (MQ=255)                                                                                                                                      aaaTTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACataata   >  1:8229/1‑141 (MQ=255)                                                                                                                                       aaTTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACATAatat  >  2:105017/1‑141 (MQ=255)                                                                                                                                           |                                                                                                                                         CAACTTTTATCTATTGGGTTTTTAGGTCAAGATGCCATAATAATTTCGAATACACCATCATATATTCACTCTACAAATGTTTTTGAGCAATTGAATTTTAGACATATTGATGTTCCTTATAATCAAATTAATGAAATTGATACCATCATTGATAGATTTATTAATTTTAAAAATAAAGCGATTTATATAGAACCTAGGTTTAATAACCCGACAGGTCGTTCTTTAACGAATGAGCAAAAGAAAAATATAATTACTTATAGCGAAAGACATAATAT  >  CP000730/124412‑124686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑