Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,385,610 A→C 8.6% intergenic (‑547/+85) fmhB ← / ← USA300HOU_2245 peptidoglycan pentaglycine interpeptide biosynthesis protein FmhB/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,385,6100AC 8.6% 106.8 / 3.2 43intergenic (‑547/+85)fmhB/USA300HOU_2245peptidoglycan pentaglycine interpeptide biosynthesis protein FmhB/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (21/18);  new base C (2/2);  total (23/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

TTATAATTATGAACATCGTTGCAATCATTATGACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGCACCTCTGATAAAAATTGAATATGGTG  >  CP000730/2385477‑2385750
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ttATAATTATGAACATCGTTGCAATCATTATGACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTaa                                                                                                                                       <  1:43745/141‑1 (MQ=255)
           aaCATCGTTGCAATCATTATGACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTTTTTTTTTTTTTTTCATCACGATTAAATAATATATTa                                                                                                                            >  2:216562/1‑141 (MQ=255)
             cATCGTTGCAATCATTATGACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAAc                                                                                                                          >  1:3192/1‑141 (MQ=255)
                cGTTGCAATCATTATGACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGAt                                                                                                                       >  1:425632/1‑141 (MQ=255)
                           ttATGACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATtg                                                                                                            >  2:112760/1‑141 (MQ=255)
                               gACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCt                                                                                                        >  2:300019/1‑141 (MQ=255)
                               gACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCt                                                                                                        >  2:334275/1‑141 (MQ=255)
                                 cATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTg                                                                                                      >  2:342902/1‑141 (MQ=255)
                                   ttGAGTCCACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCCCGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTc                                                                                                    <  2:113400/141‑1 (MQ=255)
                                    tGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCa                                                                                                   >  1:427447/1‑141 (MQ=255)
                                     gAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGTGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCat                                                                                                  <  1:16417/141‑1 (MQ=255)
                                          aaCCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTc                                                                                             <  1:226336/141‑1 (MQ=255)
                                                   ccGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCa                                                                                    >  1:199680/1‑141 (MQ=255)
                                                      tGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATtc                                                                                 <  2:150288/141‑1 (MQ=255)
                                                      tGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATtc                                                                                 <  1:342902/141‑1 (MQ=255)
                                                      tGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATtc                                                                                 <  2:422112/141‑1 (MQ=255)
                                                         aTTACTTAAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCg                                                                              >  1:360123/1‑141 (MQ=255)
                                                           tACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCt                                                                            >  1:380868/1‑141 (MQ=255)
                                                           tACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCt                                                                            >  2:35318/1‑141 (MQ=255)
                                                           tACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCt                                                                            >  1:380857/1‑141 (MQ=255)
                                                               ttAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACTATTCTTTCCATTTTGCTTGTCATTATTCATATGGGCATTCTCGCTCATa                                                                        >  2:414889/1‑141 (MQ=255)
                                                                      aCGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTa                                                                 <  1:413593/141‑1 (MQ=255)
                                                                       cGGGTTATACTTATCTTTTTTTTTTTTTTTTTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTAc                                                                >  2:14058/1‑141 (MQ=255)
                                                                       cGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTAc                                                                >  1:158656/1‑141 (MQ=255)
                                                                        gggTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACg                                                               <  1:128594/141‑1 (MQ=255)
                                                                          gTTGTACTTATCTTTTTCACATTGATATTATCAATCTTTTTCATTTTAATTAAGTCATCCCGATTAAATAATATATTAACGATTCGTTCCGTTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCGCTTACGta                                                             <  2:268305/141‑1 (MQ=255)
                                                                               aCTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATAttt                                                        <  2:181939/141‑1 (MQ=255)
                                                                               aCTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTGAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATCGCACTTACGTATAttt                                                        <  2:41452/141‑1 (MQ=255)
                                                                                  tATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTCCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTtata                                                     <  1:110006/141‑1 (MQ=255)
                                                                                        tttCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATg                                               >  1:401251/1‑141 (MQ=255)
                                                                                          tCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCCCGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGt                                             >  1:276728/1‑141 (MQ=255)
                                                                                          tCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCCCGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGt                                             >  1:238434/1‑141 (MQ=255)
                                                                                          tCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGt                                             >  2:328931/1‑141 (MQ=255)
                                                                                               tttATATTATCAATCTTTTTCATTTTTTTTTAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGTCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCaaa                                        >  1:263060/1‑141 (MQ=255)
                                                                                               tttATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCaaa                                        <  2:50016/141‑1 (MQ=255)
                                                                                               tttATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCaaa                                        <  1:125226/141‑1 (MQ=255)
                                                                                                           aTCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGc                            <  1:50054/141‑1 (MQ=255)
                                                                                                             cTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTTTGCGTGTCATTATTCTTATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGCAc                          >  1:386776/1‑141 (MQ=255)
                                                                                                                tttCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGCACctc                       <  1:7684/141‑1 (MQ=255)
                                                                                                                 ttCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCatta                                                                                                <  2:130701/67‑1 (MQ=255)
                                                                                                                 ttCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCatta                                                                                                >  1:130701/1‑67 (MQ=255)
                                                                                                                    aTTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGCACCTCTGAt                   <  1:395789/141‑1 (MQ=255)
                                                                                                                        taattaaGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGCACCTCTGATaaaa               <  1:102456/141‑1 (MQ=255)
                                                                                                                                    caCGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGCACCTCTGATAAAAATTGAATATGGt   >  1:208712/1‑141 (MQ=255)
                                                                                                                                     aCGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAGGAGCACCTCTGATAAAAATTGAATATGGTg  <  2:158656/141‑1 (MQ=255)
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TTATAATTATGAACATCGTTGCAATCATTATGACATTGAGTCAACCTCTCACCGTGAATTACTTTAGTTTACGGGTTATACTTATCTTTTTCACATTTATATTATCAATCTTTTTCATTTTAATTAAGTCATCACGATTAAATAATATATTAACGATTCTTTCCATTGTGCTTGTCATTATTCATATGGGCATTCTCGCTCATAGCACTTACGTATATTTATACTAATGGTTCAAAGCGATAAATAGCACCTCTGATAAAAATTGAATATGGTG  >  CP000730/2385477‑2385750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑