Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,372,484	A→T	L431* (TTA→TAA)	pbuG ←	NCS2 family nucleobase:cation symporter‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,372,484	0	A	T	100.0%	94.3 / NA	29	L431* (TTA→TAA)	pbuG	NCS2 family nucleobase:cation symporter‑2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (17/12);  total (17/12)

TTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGC  >  CP000730/2372361‑2372626                                                                                                                            |                                                                                                                                               ttATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTActt                                                                                                                        <  1:151064/148‑1 (MQ=255)               aTTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTa                                                                                                          <  2:235806/148‑1 (MQ=255)                 tttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTaga                                                                                                        <  1:158842/148‑1 (MQ=255)                  ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTagag                                                                                                       >  2:144668/1‑148 (MQ=255)                  ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTagag                                                                                                       >  1:235179/1‑148 (MQ=255)                  ttATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCACTCGTATTTTCTGTCTGGATTTAACCATTAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTagag                                                                                                       >  2:47728/1‑148 (MQ=255)                     tCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATa                                                                                                    >  1:162712/1‑148 (MQ=255)                                             cTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaata                                                                            >  1:73989/1‑148 (MQ=255)                                             cTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaata                                                                            >  1:341320/1‑148 (MQ=255)                                                ccAATCATTGGCAGTCGTTTTTTGTGTCTGTATTTTACTTTGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaataaat                                                                         >  2:209183/1‑148 (MQ=255)                                                ccAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACAATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAaaataaat                                                                         >  2:307662/1‑148 (MQ=255)                                                                      tgtgTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCaa                                                   <  1:338711/148‑1 (MQ=255)                                                                              gATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAatat                                           <  2:398841/148‑1 (MQ=255)                                                                              gATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAatat                                           <  2:195787/148‑1 (MQ=255)                                                                                  tAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATa                                       <  2:64576/148‑1 (MQ=255)                                                                                  tAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATa                                       >  1:217617/1‑148 (MQ=255)                                                                                  tAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATa                                       >  1:312929/1‑148 (MQ=255)                                                                                       aTGAACAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGATATAATTATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGc                                  >  2:205429/1‑148 (MQ=255)                                                                                          aaCAAATATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGcatc                               <  2:146709/148‑1 (MQ=255)                                                                                             aaaTATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATa                            >  2:227426/1‑148 (MQ=255)                                                                                             aaaTATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATa                            >  1:153358/1‑148 (MQ=255)                                                                                             aaaTATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATa                            >  2:284764/1‑148 (MQ=255)                                                                                             aaaTATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATa                            >  1:388636/1‑148 (MQ=255)                                                                                              aaTATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTTGTTATTATCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATaa                           >  2:293411/1‑148 (MQ=255)                                                                                              aaTATGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATaa                           >  2:75278/1‑148 (MQ=255)                                                                                                  tGAAGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGAATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGAt                       <  1:371449/148‑1 (MQ=255)                                                                                                     aGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGt                    <  1:342284/148‑1 (MQ=255)                                                                                                     aGTACAGAATGAATAGTACCATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGt                    <  2:6347/148‑1 (MQ=255)                                                                                                                       ccATTTATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGc  <  2:221279/148‑1 (MQ=255)                                                                                                                            |                                                                                                                                               TTATTTCGCTTCTTATTTTATCCTTACTTCAAAGAAAAAAACGACTACCAATCATTGGCAGTCGTTTTTTGTGTCTGGATTTAACCATGAACAAATATGAAGTACAGAATGAATAGTACCATTAATACATACATTATCGGATGTACTTCTTTATGTTTTTTAGAGATAAGCATTGTAATTGGATAGAAAATAAATCCACACGCAATACCAGTTGCAATTGAATATGATAAAGGCATCATAATGATTGTAATGAAGGCTGGCACTGC  >  CP000730/2372361‑2372626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑