Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,869,936	A→T	L196Q (CTG→CAG)	ytmP ←	possible kanamycin kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,869,936	0	A	T	100.0%	83.2 / NA	25	L196Q (CTG→CAG)	ytmP	possible kanamycin kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/12);  total (13/12)

TTTTGCTCTTCATACCATTGAACAAGACCTATAGATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCAGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCATGTACAACAGTGAAAAAACGCGAATCTAAACTCGGT  >  CP000730/1869792‑1870079                                                                                                                                                 |                                                                                                                                                ttttTCTCTTTATACCATTGAACAAGACCTATAGATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAgctgca                                                                                                                                              >  2:208366/1‑148 (MQ=255)         ttCATACCATTGAACAAGACCTATAGATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGata                                                                                                                                      <  1:176427/148‑1 (MQ=255)                  ttGAACAAGACCTATAGATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGcc                                                                                                                             <  1:357075/148‑1 (MQ=255)                  ttGAACAAGACCTATAGATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGcc                                                                                                                             >  1:165514/1‑148 (MQ=255)                  ttGAACAAGACCTATAGATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGcc                                                                                                                             >  1:92804/1‑148 (MQ=255)                                  gATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTg                                                                                                             <  2:10268/148‑1 (MQ=255)                                       aaTTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCt                                                                                                        <  2:55201/148‑1 (MQ=255)                                       aaTTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCt                                                                                                        <  2:132643/148‑1 (MQ=255)                                          tACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTcc                                                                                                     >  1:245775/1‑148 (MQ=255)                                              gTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAAt                                                                                                 >  1:95624/1‑148 (MQ=255)                                                    cATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCTATCATTGCACTTTCCCAATCAACTa                                                                                           >  1:284220/1‑148 (MQ=255)                                                    cATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTa                                                                                           >  1:158945/1‑148 (MQ=255)                                                         cATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCGGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAAt                                                                                      <  2:328159/148‑1 (MQ=255)                                                               tttatttaAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCa                                                                                <  1:374607/148‑1 (MQ=255)                                                                                       aaCACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTGGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAatta                                                        >  2:247117/1‑148 (MQ=255)                                                                                        acacCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAattat                                                       <  1:387270/148‑1 (MQ=255)                                                                                        acacCATATGTTTCAAGACAAACAAACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAattat                                                       <  1:3572/148‑1 (MQ=255)                                                                                                    tCAATCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACAt                                           >  1:82908/1‑148 (MQ=255)                                                                                                    tCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACAt                                           >  1:167159/1‑148 (MQ=255)                                                                                                          cATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCAt                                     <  1:135639/148‑1 (MQ=255)                                                                                                          cATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCTGCATTCCGATATCAATTGCAGGGTCTGCAATCATTGCCCCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCAt                                     <  1:247209/148‑1 (MQ=255)                                                                                                                                 aaCATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCATGTACAACAGTGAAAAAACGCGaa              >  2:70180/1‑148 (MQ=255)                                                                                                                                   cATAGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCATGTACAACAGTGAAAAAACGCGAATc            >  1:291882/1‑148 (MQ=255)                                                                                                                                      aGTTGTAAAGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCATGTACAACAGTGAAAAAACGCGAATCTaa         <  2:245775/148‑1 (MQ=255)                                                                                                                                             aaGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCATGTACAACAGTGAAAAAACGCGAATCTAAACTCGGt  >  2:120350/1‑148 (MQ=255)                                                                                                                                             aaGCTGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCATGTACAACAGTGAAAAAACGCGAATCTAAACTCGGt  >  1:118122/1‑148 (MQ=255)                                                                                                                                                 |                                                                                                                                                TTTTGCTCTTCATACCATTGAACAAGACCTATAGATTGAATTACCGTATACCATTTCATACGTTTATTTAAATTCAAGCTCTCTTGAACACCATATGTTTCAAGCCATTCAGACCATTGTTGTTGTGGAACATAGTTGTAAAGCAGCATTCCGATATCAATTGCCGGGTCTGCAATCATTGCACCTTCCCAATCAACTAAAAATAGTTCATCTCGATCGGATAATAACCAATTATTATGATTCACATCACCATGTACAACAGTGAAAAAACGCGAATCTAAACTCGGT  >  CP000730/1869792‑1870079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑