Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	CP000730	1,441,102	(TGTTG)1→2	coding (127/624 nt)	xpaC →	5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 1441097	0 (0.000)	36 (1.030) +TGTTG	16/274	0.6	100%	coding (122/624 nt)	xpaC	5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC
	?	CP000730	1441098 =	0 (0.000)					coding (123/624 nt)	xpaC	5‑bromo‑4‑chloroindolyl phosphate hydrolysis protein XpaC

AATGCTAAATAAAGGAAGATGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATTGTTGGCTTTTTAATGACATACCTACCGATACAAAAACTCACTTCACGCAAATATTTAAACGAAATTGGTTTGACTAGAAAAGACTATCGCTATATTCGAAATCAGTTAAATCATACACACCAAAAACTTAGAGGTAT  >  CP000730/1440952‑1441235                                                                                                                                                  |                                                                                                                                           aaTGCTAAATAAAGGAAGATGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtg                                                                                                                                          >  1:87049‑M1/1‑146 (MQ=255) aaTGCTAAATAAAGGAAGATGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtg                                                                                                                                          >  1:290736‑M1/1‑146 (MQ=255) aaTGCTAAATAAAGGAAGATGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtg                                                                                                                                          >  1:70424‑M1/1‑146 (MQ=255)                aaGATGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggcttttt                                                                                                                           <  2:289456‑M1/148‑18 (MQ=255)                aaGATGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggcttttt                                                                                                                           <  2:380400‑M1/148‑18 (MQ=255)                   aTGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaat                                                                                                                        <  1:352619‑M1/148‑21 (MQ=255)                   aTGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaat                                                                                                                        <  2:245166‑M1/148‑21 (MQ=255)                   aTGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaat                                                                                                                        <  2:220428‑M1/148‑21 (MQ=255)                   aTGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaat                                                                                                                        <  2:350660‑M1/148‑21 (MQ=255)                    tGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatg                                                                                                                       >  2:190046‑M1/1‑127 (MQ=255)                    tGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatg                                                                                                                       >  1:200265‑M1/1‑127 (MQ=255)                    tGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatg                                                                                                                       >  2:297072‑M1/1‑127 (MQ=255)                    tGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatg                                                                                                                       >  1:130322‑M1/1‑127 (MQ=255)                                    tatTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatac                                                                                                       <  1:345780‑M1/148‑38 (MQ=255)                                    tatTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatac                                                                                                       >  2:83024‑M1/1‑111 (MQ=255)                                    tatTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatac                                                                                                       >  2:307022‑M1/1‑111 (MQ=255)                                    tatTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtggtgtgttggctttttaatgacatacctaccgatac                                                                                                       >  1:381971‑M1/1‑111 (MQ=255)                                     atTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTGTTAATTGATATGTCGATTGGTATtgttgtgttggctttttaatgacatacctaccgataca                                                                                                      >  1:390817‑M1/1‑110 (MQ=255)                                           cATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaact                                                                                                <  1:369661‑M1/148‑45 (MQ=255)                                           cATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaact                                                                                                <  2:154649‑M1/148‑45 (MQ=255)                                                         gtTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaat                                                                                  <  1:228647‑M1/148‑59 (MQ=255)                                                         gtTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaat                                                                                  >  1:30396‑M1/1‑90 (MQ=255)                                                         gtTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaat                                                                                  >  1:234488‑M1/1‑90 (MQ=255)                                                                        tatagCATTTTTAACAAGCATATTTGTGATTATTGCACGTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacataaaaaaaaaaaaaaaaactcacttcacgcaaatatttaaacgaaattg                                                                   <  2:300862‑M1/146‑74 (MQ=255)                                                                        tGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattg                                                                   <  1:144507‑M1/148‑74 (MQ=255)                                                                        tGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattg                                                                   <  1:205592‑M1/148‑74 (MQ=255)                                                                                ttttAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattggtttgact                                                           >  1:159233‑M1/1‑67 (MQ=255)                                                                                ttttAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattggtttgact                                                           >  1:279907‑M1/1‑67 (MQ=255)                                                                                           atatTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattggtttgactagaaaagacta                                                >  1:107236‑M1/1‑56 (MQ=255)                                                                                           atatTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgtgggctgtttaattacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattggtttgactagaaaagacta                                                >  1:370151‑M1/1‑56 (MQ=255)                                                                                                        ttGCACTTGATGTATCTTTTTTATTTGATTTTTCTATTGGTAGtggtgtggtggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaactaaattggtttgactagaaaagactatctctatattcga                                   >  2:370128‑M1/1‑43 (MQ=255)                                                                                                        ttGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactaaattaacgaaaatatttaaacgaaattggtttgactagaaaagactatcgctatattcga                                   >  1:102472‑M1/1‑43 (MQ=255)                                                                                                        ttGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGNATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattggtttgactagaaaagactatggctatattcga                                   >  1:319468‑M1/1‑43 (MQ=255)                                                                                                          gCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggcttttttatttcttacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattggtttgactagaaaagactatcgctatattcgaaa                                 >  2:33401‑M1/1‑41 (MQ=255)                                                                                                              ttGATGTATCTTTTTTAATTGATATGTCTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacttcacgcaaatatttaaacgaaattggtttgactagaaaagactatcgctatattcgaaatcag                             >  2:167914‑M1/1‑37 (MQ=255)                                                                                                                                         cTATTGGTATtgttgtgttggctttttaatgacatacctaccgatacaaaaactcacctcacgcaaatatttaaacgaaattggtttgactagaaaagactatcgctatattcgaaatcagttaaatcatacacaccaaaaacctaga  >  2:86232‑M1/1‑10 (MQ=255)                                                                                                                                                  |                                                                                                                                           AATGCTAAATAAAGGAAGATGACAGTGAGATATAATATTTCTCATATATTTGGGGTGTTAGTGGGAATTCCTGTAGCGTTTTTAACAAGCATATTTGGGATGATTGCACTTGATGTATCTTTTTTAATTGATATGTCTATTGGTATTGTTGGCTTTTTAATGACATACCTACCGATACAAAAACTCACTTCACGCAAATATTTAAACGAAATTGGTTTGACTAGAAAAGACTATCGCTATATTCGAAATCAGTTAAATCATACACACCAAAAACTTAGAGGTAT  >  CP000730/1440952‑1441235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.