Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,653,327	A→G	5.7%	intergenic (‑32/+127)	gcvT ← / ← aroK	aminomethyltransferase/shikimate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,653,327	0	A	G	5.7%	217.7 / 3.2	72	intergenic (‑32/+127)	gcvT/aroK	aminomethyltransferase/shikimate kinase
Reads supporting (aligned to +/- strand):  ref base A (34/33);  new base G (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

AATACTTGAAAATTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGATCAC  >  CP000730/1653188‑1653467                                                                                                                                            |                                                                                                                                             aaTACTTGAAAATTGAACAGGCAGCGCCCATCCTCCGAATTCCACAATTTTTGCACGTCTATGAAGATTATTTTTATATAAAGGTGGTTGTTTTATTTGGCTTGACAGGGGAATACCGCCTTGAAATGATAAAAACAGGag                                                                                                                                             >  1:709593/1‑140 (MQ=255)  aTACTTGAAAATTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATa                                                                                                                                            >  1:249154/1‑141 (MQ=255)    aCTTGAAAATTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGc                                                                                                                                          <  1:376295/141‑1 (MQ=255)     cTTGAAAATTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCa                                                                                                                                         <  1:349726/141‑1 (MQ=255)      ttGAAAATTGAACAGGCATCGCCCATCCTCCGCATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAATATTATAAAAACAGGATAGCaa                                                                                                                                        <  2:277691/141‑1 (MQ=255)      ttGAAAATTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCaa                                                                                                                                        >  2:58455/1‑141 (MQ=255)            aTTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTa                                                                                                                                  <  2:482849/141‑1 (MQ=255)            aTTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTa                                                                                                                                  <  1:444618/141‑1 (MQ=255)             ttGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTAc                                                                                                                                 <  1:36372/141‑1 (MQ=255)               gAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTAccc                                                                                                                               >  1:263602/1‑141 (MQ=255)                  cAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGt                                                                                                                            <  2:455432/141‑1 (MQ=255)                  cAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGt                                                                                                                            <  2:455406/141‑1 (MQ=255)                  cAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGt                                                                                                                            >  1:277328/1‑141 (MQ=255)                    ggCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGttt                                                                                                                          >  2:244088/1‑141 (MQ=255)                    ggCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGttt                                                                                                                          >  1:681778/1‑141 (MQ=255)                    ggCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGttt                                                                                                                          >  2:474113/1‑141 (MQ=255)                     gCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTta                                                                                                                         >  1:529393/1‑141 (MQ=255)                              aTCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATCTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCAcc                                                                                                                <  2:441696/141‑1 (MQ=255)                                cctccGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGGTAGcgattgct                                                                                                                                   <  2:61733/120‑8 (MQ=255)                                cctccGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGGTAGCGATTGCt                                                                                                                                   >  1:61733/1‑120 (MQ=255)                                 ctccGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAAt                                                                                                             >  1:263021/1‑141 (MQ=255)                                    cGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCa                                                                                                          >  1:108879/1‑141 (MQ=255)                                      aaTTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAgg                                                                                                        <  1:209238/141‑1 (MQ=255)                                       aTTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGa                                                                                                       >  1:582438/1‑141 (MQ=255)                                           cacaATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAAcgc                                                                                                   <  2:277328/141‑1 (MQ=255)                                                tttttGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTAc                                                                                              <  1:101892/141‑1 (MQ=255)                                                    tGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGGTAGcg                                                                                                                                         >  1:576592/1‑93 (MQ=255)                                                    tGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGGTAGcg                                                                                                                                         <  2:576592/94‑2 (MQ=255)                                                     gCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTCATAAATCACCAATTCAGGAACGCGTTACAGtat                                                                                         <  1:523948/141‑1 (MQ=255)                                                         ctctATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCCTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGtattacc                                                                                     <  2:302826/141‑3 (MQ=255)                                                             aTCAACATAATTTTGATATAAAGGTGGTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACGGGATAGCAATCGCTACCCTGTTTATAAATCACCTATTCAGGAGCGCGTGACAGTATTATCCttt                                                                                 <  2:284437/141‑1 (MQ=255)                                                                  cATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCt                                                                            >  1:196464/1‑141 (MQ=255)                                                                  cATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCt                                                                            <  1:294838/141‑1 (MQ=255)                                                                  cATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCt                                                                            >  2:338873/1‑141 (MQ=255)                                                                  cATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCACCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCt                                                                            >  1:701299/1‑141 (MQ=255)                                                                     aaTTTTGATATAAAGGTGTTTGTTTTCAATCACTTGACCTTGCACTACCCCCTTAACATCATACACACAGGTAAGCCATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTgag                                                                         <  1:242519/141‑1 (MQ=255)                                                                      aTTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTgaga                                                                        >  1:525904/1‑141 (MQ=255)                                                                       ttttGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGagag                                                                       <  1:58455/141‑1 (MQ=255)                                                                         ttGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGtt                                                                     <  1:34625/141‑1 (MQ=255)                                                                         ttGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGtt                                                                     >  1:347524/1‑141 (MQ=255)                                                                         ttGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGtt                                                                     >  2:128649/1‑141 (MQ=255)                                                                          tGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGttt                                                                    >  1:127778/1‑141 (MQ=255)                                                                            atatAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCg                                                                  >  2:75490/1‑141 (MQ=255)                                                                                 aaGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGtaata                                                             >  2:284342/1‑141 (MQ=255)                                                                                  aGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGtaataa                                                            <  1:199686/141‑1 (MQ=255)                                                                                    gtgtTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAAt                                                          <  2:164994/141‑1 (MQ=255)                                                                                          gtttTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTg                                                    <  2:194769/141‑1 (MQ=255)                                                                                               aaaTCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCt                                               <  2:300819/141‑1 (MQ=255)                                                                                               aaaTCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCt                                               <  1:699512/141‑1 (MQ=255)                                                                                                aaTCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCtt                                              >  2:423732/1‑141 (MQ=255)                                                                                                        gACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATa                                      >  2:611435/1‑141 (MQ=255)                                                                                                         aCATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATag                                     >  2:544713/1‑141 (MQ=255)                                                                                                              gAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCt                                <  2:127778/141‑1 (MQ=255)                                                                                                               aaaTACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCtt                               >  1:163244/1‑141 (MQ=255)                                                                                                                   aCCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACg                           >  2:545958/1‑141 (MQ=255)                                                                                                                    cccccTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGc                          >  2:16961/1‑141 (MQ=255)                                                                                                                    cccccTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGc                          >  2:188984/1‑141 (MQ=255)                                                                                                                    cccccTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGc                          >  2:11788/1‑141 (MQ=255)                                                                                                                     ccccTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGct                         >  1:112514/1‑141 (MQ=255)                                                                                                                            aaaTTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAAATCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATtct                  <  1:137111/141‑1 (MQ=255)                                                                                                                             aaTTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATtctc                 <  1:423732/141‑1 (MQ=255)                                                                                                                             aaTTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATtctc                 <  1:474113/141‑1 (MQ=255)                                                                                                                               ttATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATtctctc               <  2:474510/141‑1 (MQ=255)                                                                                                                               ttATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATtctctc               <  2:701299/141‑1 (MQ=255)                                                                                                                                tataAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTctctct              >  1:128298/1‑141 (MQ=255)                                                                                                                                tataAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTctctct              >  2:282833/1‑141 (MQ=255)                                                                                                                                     aaaCAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATAc         >  2:310385/1‑141 (MQ=255)                                                                                                                                      aaCAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACt        <  2:263602/141‑1 (MQ=255)                                                                                                                                      aaCAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACt        <  1:712268/141‑1 (MQ=255)                                                                                                                                       aCAGGATAGCAATCGCTACCCTTTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTg       <  2:402724/141‑1 (MQ=255)                                                                                                                                        cAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGa      >  1:83025/1‑141 (MQ=255)                                                                                                                                          ggATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGATc    <  2:681778/141‑1 (MQ=255)                                                                                                                                           gATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGATCa   >  1:30663/1‑141 (MQ=255)                                                                                                                                            aTAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGATCAc  <  2:112346/141‑1 (MQ=255)                                                                                                                                            aTAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGATCAc  <  2:196464/141‑1 (MQ=255)                                                                                                                                            aTAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGATCAc  <  1:92323/141‑1 (MQ=255)                                                                                                                                            |                                                                                                                                             AATACTTGAAAATTGAACAGGCATCGCCCATCCTCCGAATTCCACAATTTTTGCACCTCTATCAACATAATTTTGATATAAAGGTGTTTGTTTTAAATCACTTGACATTGAAATACCCCCTTAAAATTATAAAAACAGGATAGCAATCGCTACCCTGTTTATAAATCACCAATTCAGGAACGCGTTACAGTATTATCCTTTTGCCTGAGAGTTTCGTAATAAATTACTTGCACCTTCGGCGATAGAGTCTTAACGCTCTATTCTCTCTAATACTGATCAC  >  CP000730/1653188‑1653467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑