Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,289,745	C→T	5.3%	P7L (CCG→CTG)	ylxR →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,289,745	0	C	T	5.3%	234.8 / 2.9	76	P7L (CCG→CTG)	ylxR	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (37/34);  new base T (2/2);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01

GAAGAAGATGTTGCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGGCGCATATGTTTCTAAA  >  CP000730/1289607‑1289878                                                                                                                                           |                                                                                                                                      gaagaaGATGTTGCTTCATAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGa                                                                                                                                     <  1:572343/141‑1 (MQ=255)    gaagaTGTTGCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGc                                                                                                                                  <  1:637065/141‑1 (MQ=255)          gTTGCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAAt                                                                                                                            >  2:31454/1‑141 (MQ=255)          gTTGCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAAt                                                                                                                            <  1:509908/141‑1 (MQ=255)           ttGCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATg                                                                                                                           >  2:377921/1‑141 (MQ=255)           ttGCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATg                                                                                                                           >  1:462471/1‑141 (MQ=255)             gCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTa                                                                                                                         <  1:177719/141‑1 (MQ=255)             gCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTa                                                                                                                         >  2:256763/1‑141 (MQ=255)                   gaagaTGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCttt                                                                                                                   >  1:705496/1‑141 (MQ=255)                      gaTGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCaa                                                                                                                <  1:681746/141‑1 (MQ=255)                       aTGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCaaa                                                                                                               >  1:157520/1‑141 (MQ=255)                        tGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACACATGTAGAGAAAGAATCTGAATAATAGGTTGGAGCGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCaaat                                                                                                              >  1:526394/1‑141 (MQ=255)                         gCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCaaatg                                                                                                             <  1:111581/141‑1 (MQ=255)                           tGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCaaatgaa                                                                                                           <  2:589782/141‑1 (MQ=255)                               acaacaGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGc                                                                                                       >  1:205532/1‑141 (MQ=255)                               acaacaGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGc                                                                                                       >  1:562276/1‑141 (MQ=255)                                 aacaGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCAt                                                                                                     <  1:448955/141‑1 (MQ=255)                                  acaGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATc                                                                                                    >  1:235740/1‑141 (MQ=255)                                     gAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCa                                                                                                 >  2:515036/1‑141 (MQ=255)                                        tCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCaaaa                                                                                              >  1:154322/1‑141 (MQ=255)                                        tCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCaaaa                                                                                              >  1:85749/1‑141 (MQ=255)                                          aaCCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCaaaaaa                                                                                            >  2:215099/1‑141 (MQ=255)                                           aCCGAAGAGGGAAATGATGTATCAGTTGACACAAATGGAAAGAGAGAAACTGAATAATAGGTTGGAGTGAAGTATGTATGAAAAAGAAAAAAATTCCGAAGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAg                                                                                           >  2:668053/1‑141 (MQ=255)                                                agagGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATg                                                                                      <  1:200384/141‑1 (MQ=255)                                                   ggTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGAtt                                                                                   >  1:298410/1‑141 (MQ=255)                                                   ggTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGAtt                                                                                   <  1:31454/141‑1 (MQ=255)                                                   ggTAAATGATGTTTCAGTTGAAACAAATGCAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGACAATGTATTCTCTCAAATGAAATGCATCCCAAAAACGATATGaat                                                                                   >  2:160436/1‑139 (MQ=255)                                                      aaaTGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCgt                                                                                >  2:696914/1‑141 (MQ=255)                                                           atgTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGtgttgt                                                                           <  1:646300/141‑1 (MQ=255)                                                               ttCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTgttgttgtt                                                                       <  1:299674/141‑1 (MQ=255)                                                               ttCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTgttgttgtt                                                                       <  2:542970/141‑1 (MQ=255)                                                                  aGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTtaat                                                                    >  1:368302/1‑141 (MQ=255)                                                                  aGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAAAAAAAAAAAAAAAAGATATGATTCGTGTTGTTGTtaat                                                                    <  2:469509/141‑1 (MQ=255)                                                                   gTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTtaata                                                                   <  2:591769/141‑1 (MQ=255)                                                                        aaCAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATGCCTAAGCTAAAATTTATTCGTACAAAGGGAATGCAGCCCAAAAAAGAGATGATTCGTGTTGTTGTTAATAaagaa                                                              >  1:601636/1‑141 (MQ=255)                                                                           aaaTGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGc                                                           <  1:652181/141‑1 (MQ=255)                                                                            aaTGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCg                                                          >  2:274254/1‑141 (MQ=255)                                                                            aaTGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTAT‑AAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGa                                                         <  2:163481/141‑1 (MQ=255)                                                                             aTGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGa                                                         >  2:364183/1‑141 (MQ=255)                                                                               gTAGAGAAAGAATCTGAATAATAGGTTAGAGGGAGGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGaaa                                                       >  1:339577/1‑141 (MQ=255)                                                                                 agagAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATc                                                     >  1:367347/1‑141 (MQ=255)                                                                                  gagaAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCt                                                    <  1:30027/141‑1 (MQ=255)                                                                                    gaaagaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCttt                                                  >  2:449874/1‑141 (MQ=255)                                                                                     aaagaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTg                                                 >  1:564905/1‑141 (MQ=255)                                                                                      aagaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGc                                                <  2:590455/141‑1 (MQ=255)                                                                                       agaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCg                                               <  1:537723/141‑1 (MQ=255)                                                                                        gaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCTGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCg                                                                                 >  1:374816/1‑106 (MQ=255)                                                                                        gaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCTGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCg                                                                                 >  1:374829/1‑106 (MQ=255)                                                                                        gaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCTGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCg                                                                                 <  2:374829/106‑1 (MQ=255)                                                                                        gaaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCTGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCg                                                                                 <  2:374816/106‑1 (MQ=255)                                                                                         aaTCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGa                                             <  2:162595/141‑1 (MQ=255)                                                                                            cTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGt                                          <  1:377921/141‑1 (MQ=255)                                                                                              gAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAACCAGAAATCTTTGCGGATGTTa                                        >  1:180592/1‑141 (MQ=255)                                                                                               aataatAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTAc                                       <  2:237141/141‑1 (MQ=255)                                                                                               aataatAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAANAAAGAAGGCGAANTCTTTGCGGATGTTAc                                       <  1:703944/141‑1 (MQ=255)                                                                                               aataatAGGTTGGAGTGAAATATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTAc                                       <  1:127716/141‑1 (MQ=255)                                                                                                 aaataGGTTGGAGTGAAATATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAAAAAAAAAAAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTg                                     <  2:555480/140‑1 (MQ=255)                                                                                                  aataGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTgg                                    >  1:275497/1‑141 (MQ=255)                                                                                                   ataGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGga                                   <  2:157520/141‑1 (MQ=255)                                                                                                       gTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGGAGGCGAAATCTTTGCGGATGTTACTGgaaaga                               >  2:120371/1‑141 (MQ=255)                                                                                                       gTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGgaaaga                               >  2:7264/1‑141 (MQ=255)                                                                                                       gTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGgaaaga                               >  2:178424/1‑141 (MQ=255)                                                                                                             gTGAAGTATCTATGAAGAAGAAAAAAATTCAGATGCGAAATTGTATTCTTTTAAATGGAATGCAAGCCAAAAAAGATTGGATTCGTGTTGTTGATAATAAAGAAAGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAg                         >  2:26817/1‑141 (MQ=255)                                                                                                             aaatgttATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAg                         <  1:176215/135‑1 (MQ=255)                                                                                                               gAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGc                       >  2:675102/1‑141 (MQ=255)                                                                                                                 aGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCg                     <  2:235740/141‑1 (MQ=255)                                                                                                                    agcTATGAAAAAGAAATAAAGTCCGAAGCGAAAATGTATTCTTTCAAATGAAATGCATACCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTgg                  <  2:387850/139‑1 (MQ=255)                                                                                                                    aTCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTgg                  <  1:168976/141‑1 (MQ=255)                                                                                                                      cTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGgcg                >  2:227819/1‑141 (MQ=255)                                                                                                                         tGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGGCGCat             <  1:695070/141‑1 (MQ=255)                                                                                                                            aaaagaaaaaAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGGCGCATATg          >  1:167922/1‑141 (MQ=255)                                                                                                                              aagaaaaaAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATAAAAACAAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGGCGCATATGtt        <  2:672210/141‑1 (MQ=255)                                                                                                                               agaaaaaAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGGCGCATATGttt       >  2:573585/1‑141 (MQ=255)                                                                                                                                    aaaaTTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGGCGCATATGTTTCTaaa  >  1:58807/1‑141 (MQ=255)                                                                                                                                       aTTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTgttgttgtt                                                                       <  2:502702/69‑1 (MQ=255)                                                                                                                                       aTTCCGATGCGAAAATGTATTCTTGCAAATGAAATGCATCCCAAAAAAGATATGATTCGTgttgttgtt                                                                       >  1:502702/1‑69 (MQ=255)                                                                                                                                           |                                                                                                                                      GAAGAAGATGTTGCTTTAGAAGATGCTGACACAACAGAATCAACCGAAGAGGTAAATGATGTTTCAGTTGAAACAAATGTAGAGAAAGAATCTGAATAATAGGTTGGAGTGAAGTATCTATGAAAAAGAAAAAAATTCCGATGCGAAAATGTATTCTTTCAAATGAAATGCATCCCAAAAAAGATATGATTCGTGTTGTTGTTAATAAAGAAGGCGAAATCTTTGCGGATGTTACTGGAAAGAAACAAGGCCGTGGCGCATATGTTTCTAAA  >  CP000730/1289607‑1289878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑