Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,178,095 C→G 5.9% S148C (TCT→TGT)  arcD2 → arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,178,0950CG 5.9% 221.5 / 4.9 68S148C (TCT→TGT) arcD2arginine/ornithine APC family amino acid‑polyamine‑organocation transporter, antiporter
Reads supporting (aligned to +/- strand):  ref base C (37/26);  new base G (2/2);  total (40/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

TTATGAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGTAGCATTTTATCCGA  >  CP000730/1177955‑1178232
                                                                                                                                            |                                                                                                                                         
ttATGAAAGATTAAAGCAACATCCCGCTGGACCAGAACAAATAACTAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTc                                                                                                                                           <  1:685708/141‑1 (MQ=255)
 tATGAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCt                                                                                                                                          <  1:280326/141‑1 (MQ=255)
 tATGAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCt                                                                                                                                          <  1:451914/141‑1 (MQ=255)
  aTGAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCtt                                                                                                                                         <  1:166238/141‑1 (MQ=255)
        gATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAAt                                                                                                                                   >  1:288014/1‑141 (MQ=255)
         aTTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATc                                                                                                                                  >  2:309501/1‑141 (MQ=255)
           tAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCgg                                                                                                                                >  1:624458/1‑141 (MQ=255)
             aaGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTc                                                                                                                                           <  2:297028/128‑1 (MQ=255)
             aaGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTc                                                                                                                                           >  1:297028/1‑128 (MQ=255)
             aaGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGa                                                                                                                              <  1:616132/141‑1 (MQ=255)
              aGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGAt                                                                                                                             >  2:553713/1‑141 (MQ=255)
              aGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGAt                                                                                                                             >  2:523594/1‑141 (MQ=255)
              aGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGAt                                                                                                                             >  1:162251/1‑141 (MQ=255)
                 aaCATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGt                                                                                                                          >  1:176367/1‑141 (MQ=255)
                  aCATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGtt                                                                                                                         >  2:345800/1‑141 (MQ=255)
                  aCATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGtt                                                                                                                         >  2:665213/1‑141 (MQ=255)
                   cATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTa                                                                                                                        >  1:318450/1‑141 (MQ=255)
                    aTCCAGCTGGACCAGAACAAATAACAGGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAg                                                                                                                       <  1:497998/141‑1 (MQ=255)
                     tCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGc                                                                                                                      <  1:69475/141‑1 (MQ=255)
                      ccAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCt                                                                                                                     >  2:331549/1‑141 (MQ=255)
                        aGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCttt                                                                                                                   <  2:297121/141‑1 (MQ=255)
                          cTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTaa                                                                                                                 <  2:478519/141‑1 (MQ=255)
                          cTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTaa                                                                                                                 <  1:46105/141‑1 (MQ=255)
                                  gAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGaaa                                                                                                         <  2:407221/141‑1 (MQ=255)
                                  gAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGaaa                                                                                                         >  1:446572/1‑141 (MQ=255)
                                  gAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGaaa                                                                                                         >  2:589985/1‑141 (MQ=255)
                                   aaCAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGaaaa                                                                                                        <  1:481739/141‑1 (MQ=255)
                                       aaTAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACaaa                                                                                                    >  1:353027/1‑141 (MQ=255)
                                         tAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGTATTGTTAGCTTTAACGAAGAAAACACAAg                                                                                                  >  2:670103/1‑141 (MQ=255)
                                         tAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGCCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAg                                                                                                  >  2:39026/1‑141 (MQ=255)
                                          aaCAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAgg                                                                                                 >  1:94205/1‑141 (MQ=255)
                                          aaCAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAgg                                                                                                 >  1:436581/1‑141 (MQ=255)
                                              aGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCAt                                                                                             >  1:128826/1‑141 (MQ=255)
                                               gtggCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATg                                                                                            <  2:93303/138‑1 (MQ=255)
                                                 aGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGaa                                                                                          >  2:221578/1‑141 (MQ=255)
                                                            aGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAAt                                                                               <  2:105218/141‑1 (MQ=255)
                                                              gTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATtg                                                                             <  1:39240/141‑1 (MQ=255)
                                                                 cGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTgtgt                                                                          <  1:331549/141‑1 (MQ=255)
                                                                    tAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTg                                                                       >  1:186778/1‑141 (MQ=255)
                                                                        aGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATc                                                                   <  1:286729/141‑1 (MQ=255)
                                                                            gTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATa                                                               >  1:494619/1‑141 (MQ=255)
                                                                            gTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATa                                                               <  1:43755/141‑1 (MQ=255)
                                                                             tCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATAc                                                              >  2:488475/1‑141 (MQ=255)
                                                                              cGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACt                                                             >  2:106908/1‑141 (MQ=255)
                                                                                atatCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTaa                                                           >  1:204866/1‑141 (MQ=255)
                                                                                      tGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGt                                                                                                                          <  1:224236/72‑1 (MQ=255)
                                                                                      tGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGt                                                                                                                          >  2:224236/1‑72 (MQ=255)
                                                                                      tGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTa                                                     >  2:224471/1‑141 (MQ=255)
                                                                                             cattcTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATcggcgg                                              >  1:508638/1‑141 (MQ=255)
                                                                                             cattcGTGTACTAGGGTGACTTATTGTCGTAGTTCAAGCCAGTGGTGCTTTTGAGTCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGACAGGAATTTATGCTTATTGGGTTTGCATCAATACTAATGATTATcggcgg                                              <  2:435028/141‑1 (MQ=255)
                                                                                               ttcTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGa                                            >  2:21297/1‑141 (MQ=255)
                                                                                                tcTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGAc                                           >  2:413444/1‑141 (MQ=255)
                                                                                                      aCTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTAtg                                     <  2:340429/141‑1 (MQ=255)
                                                                                                       cTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTAtgt                                    <  1:597367/141‑1 (MQ=255)
                                                                                                        tAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTAtgtg                                   >  1:447394/1‑141 (MQ=255)
                                                                                                          gggggACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGt                                 <  2:170336/141‑1 (MQ=255)
                                                                                                                 ttATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTgaag                          >  2:591857/1‑141 (MQ=255)
                                                                                                                 ttATTGGCGTAGTTCAAGCCAGTGGTTATTTTGGATCGGGCTTGTTAGCTTTAACGAAGAAACCAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTAtatcacagtcaag                          >  2:560168/1‑129 (MQ=255)
                                                                                                                  tATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGCTTATCGGCGGGTCATTATGTGGTATTgaaga                         <  2:353027/141‑1 (MQ=255)
                                                                                                                   aTTGGCGTAGTTCAAGCCAGTGGTTGTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTgaagaa                        >  1:82493/1‑141 (MQ=255)
                                                                                                                   aTTGGCGTAGTTCAAGCCAGTGGTTGTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTgaagaa                        >  1:82505/1‑141 (MQ=255)
                                                                                                                   aTTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTgaagaa                        <  1:706218/141‑1 (MQ=255)
                                                                                                                        cGTAGTTCAATCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGc                   <  2:25456/141‑1 (MQ=255)
                                                                                                                           aGTTCAAGCCAGTGGTTGTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGt                <  2:82505/141‑1 (MQ=255)
                                                                                                                           aGTTCAAGCCAGTGGTTGTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGt                <  2:82493/141‑1 (MQ=255)
                                                                                                                              tCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGGAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGTAGc             >  1:588200/1‑141 (MQ=255)
                                                                                                                                  gCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGTAGCAttt         >  2:558601/1‑141 (MQ=255)
                                                                                                                                    cAGCGGTTCATTTGAATCGGGATTGCGAGCCTTACCGCAGCAAACACAAGGGCATGAATTTATGCTAAGTGTGTTGGTACCAATACTAATGATTATCGGCACGACATTATGTGGGATGGAAGAAGAAGATGTAGCCTTTTa       >  2:328573/1‑141 (MQ=255)
                                                                                                                                      gTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGTAGCATTTTATc     >  2:107261/1‑141 (MQ=255)
                                                                                                                                        ggTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGTAGCATTTTATCCg   >  1:99465/1‑141 (MQ=255)
                                                                                                                                         gTTCTTTTGAATCGGGGTTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGTGACATTATGTGGTATTGAAGAAGAAGCTGTAGCATTTTATCCGa  <  2:50243/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                         
TTATGAAAGATTAAAGCAACATCCAGCTGGACCAGAACAAATAACAAGTAGCATGGTTGAAGGTACGATAGAAGCGGTCGATATCATGGTATTCATTCTTGTACTAGGGGGACTTATTGGCGTAGTTCAAGCCAGTGGTTCTTTTGAATCGGGATTGTTAGCTTTAACGAAGAAAACAAAAGGGCATGAATTTATGCTAATTGTGTTTGTATCAATACTAATGATTATCGGCGGGACATTATGTGGTATTGAAGAAGAAGCTGTAGCATTTTATCCGA  >  CP000730/1177955‑1178232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑