Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 846,387 A→T 5.4% S278S (TCA→TCT)  yvcD → hypothetical TPR domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730846,3870AT 5.4% 240.5 / 3.3 74S278S (TCA→TCT) yvcDhypothetical TPR domain protein
Reads supporting (aligned to +/- strand):  ref base A (33/37);  new base T (2/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

ATTTAAAAATATTAAACAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTGGGTAATTGAAAATAGCAA  >  CP000730/846248‑846524
                                                                                                                                           |                                                                                                                                         
atTTAAAAATATTAAACAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCaa                                                                                                                                          >  1:49297/1‑141 (MQ=255)
atTTAAAAATATTAAACAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCaa                                                                                                                                          <  1:594946/141‑1 (MQ=255)
 tttAAAAATATTAAACAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAAt                                                                                                                                         <  1:167783/141‑1 (MQ=255)
      aaaTATTAAACAAAGTTGTACCTATGAATGACGATGAAAGTTTTATATTAGGTATTGTATTAAGTTATTTAAAGCAGGATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCaaa                                                                                                                                    <  2:478885/141‑1 (MQ=255)
          atTAAACAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATAACAACTATTGTTGTAAAAAAAAAAAAAAAAAGGGAAATTTTTATCAATTCAAATGTa                                                                                                                                <  2:467932/141‑1 (MQ=255)
             aaaCAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACaa                                                                                                                             <  2:486575/141‑1 (MQ=255)
             aaaCAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACaa                                                                                                                             >  2:15638/1‑141 (MQ=255)
              aaCAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAAt                                                                                                                            <  2:246281/141‑1 (MQ=255)
              aaCAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATGAGGTATTGTATTAAGTCATTTAAAGCAGTATCGTGCATCACAACAAGTGTTGTACCCTTTATATAAAAAAGCGGAAGTTTTATCAGTTCAAAAATACAAt                                                                                                                            >  2:493799/1‑141 (MQ=255)
                    gTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTa                                                                                                                      >  1:284986/1‑141 (MQ=255)
                    gTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTa                                                                                                                      >  2:132044/1‑141 (MQ=255)
                      tGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGc                                                                                                                    <  2:266765/141‑1 (MQ=255)
                          ccTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCTATTCAAATGTACAATGCTTTAGCatat                                                                                                                >  2:655493/1‑141 (MQ=255)
                          ccTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCTATTCAAATGTACAATGCTTTAGCatat                                                                                                                >  2:301183/1‑141 (MQ=255)
                                 atgaCGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTAAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCGTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAattatt                                                                                                         <  2:457661/141‑1 (MQ=255)
                                      gATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTTTCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAattattattat                                                                                                    >  1:581622/1‑141 (MQ=255)
                                       aTGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAattattattatt                                                                                                   >  2:96303/1‑141 (MQ=255)
                                           aaGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAgg                                                                                               >  1:188862/1‑141 (MQ=255)
                                               tttAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTgaa                                                                                           >  1:697156/1‑141 (MQ=255)
                                                   aaTTATGTATTGTATTAATTTATTTAAAGCATTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGCGaagaag                                                                                       <  1:627109/141‑1 (MQ=255)
                                                    aTTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAagaaga                                                                                      >  2:152446/1‑141 (MQ=255)
                                                             tgtattAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCa                                                                             <  2:240436/141‑1 (MQ=255)
                                                              gtattAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCAt                                                                            <  2:49297/141‑1 (MQ=255)
                                                                     gTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTactact                                                                     <  1:267967/141‑1 (MQ=255)
                                                                       tATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTgg                                                                   <  2:88399/141‑1 (MQ=255)
                                                                        aTTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTggg                                                                  >  1:625548/1‑141 (MQ=255)
                                                                        aTTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTggg                                                                  >  2:658742/1‑141 (MQ=255)
                                                                         tttAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGa                                                                 <  1:292382/141‑1 (MQ=255)
                                                                         tttAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGa                                                                 <  1:547651/141‑1 (MQ=255)
                                                                         tttAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGa                                                                 <  1:480816/141‑1 (MQ=255)
                                                                         tttAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGa                                                                 >  1:547408/1‑141 (MQ=255)
                                                                         attAAAGCAGTATCGTGCATCACAACAATTGTTATACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTAAAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGa                                                                 <  2:232950/140‑1 (MQ=255)
                                                                          ttAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGAt                                                                <  2:267552/141‑1 (MQ=255)
                                                                             aaGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGACAACTAAAGTCATTACTACTTGGATaaa                                                             >  2:65586/1‑141 (MQ=255)
                                                                             aaGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATaaa                                                             >  1:233636/1‑141 (MQ=255)
                                                                               gCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAAtt                                                           >  1:528428/1‑141 (MQ=255)
                                                                               gCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAAtt                                                           >  2:334802/1‑141 (MQ=255)
                                                                               gCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAAtt                                                           >  2:227488/1‑141 (MQ=255)
                                                                                 aGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATAATTATTGTTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGa                                                         <  1:566290/141‑1 (MQ=255)
                                                                                  gTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGaa                                                        >  2:547392/1‑141 (MQ=255)
                                                                                    aTCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGc                                                      <  1:54094/141‑1 (MQ=255)
                                                                                    aTCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGc                                                      >  1:576786/1‑141 (MQ=255)
                                                                                    aTCGCGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGc                                                      >  2:190279/1‑141 (MQ=255)
                                                                                     tCGTGCATCACAACAATTGTTGTATCCTTTATGTATAAAAGAGAAATTTTGATCAATTTAAATGTACAACTCTTTAGCATCTAATTTTTATTATTTAGATGAAGAAGACGAAACTCACAACTACTGGGTAGAATTGATGCa                                                     >  2:654298/1‑141 (MQ=255)
                                                                                        tGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTCGCTGAATAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAAt                                                  <  1:336292/141‑1 (MQ=255)
                                                                                        tGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAAt                                                  <  1:49018/141‑1 (MQ=255)
                                                                                        tGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAAt                                                  <  2:250334/141‑1 (MQ=255)
                                                                                        tGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAAt                                                  <  1:70204/141‑1 (MQ=255)
                                                                                         gCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAAtt                                                 <  2:145240/141‑1 (MQ=255)
                                                                                              acaacaATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTaa                                            >  1:486594/1‑141 (MQ=255)
                                                                                                aacaaTTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAg                                          <  2:462457/141‑1 (MQ=255)
                                                                                                aacaaTTGTTGTACCCTATAAATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAg                                          <  2:631379/141‑1 (MQ=255)
                                                                                                aacaaTTGTTGTAACAAAAAAATAAAAAAGGGAAATTTTTATCAATTCAAATATACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAg                                          <  2:631378/141‑1 (MQ=255)
                                                                                                         tgtACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTg                                 <  1:245985/141‑1 (MQ=255)
                                                                                                            aCCCTTTATATAAAAAAGGGAAATTTTTATCTATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGAc                              <  1:301183/141‑1 (MQ=255)
                                                                                                            aCCCTTTATATAAAAAAGGGAAATTTTTATCTATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGAc                              <  1:655493/141‑1 (MQ=255)
                                                                                                               cttttttatataaaGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATg                           >  1:393618/12‑141 (MQ=255)
                                                                                                                tttATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATgc                          >  1:69848/1‑141 (MQ=255)
                                                                                                                   atataAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGcc                       <  1:337243/141‑1 (MQ=255)
                                                                                                                    tataAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCt                      <  2:47441/141‑1 (MQ=255)
                                                                                                                    tataAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCt                      >  1:285238/1‑141 (MQ=255)
                                                                                                                    tataAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCt                      <  2:47471/141‑1 (MQ=255)
                                                                                                                    tataAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCt                      >  2:573355/1‑141 (MQ=255)
                                                                                                                      taAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTg                    <  1:384322/141‑1 (MQ=255)
                                                                                                                      taAAAAAGGGAAATTTTTATCAATTCAAATGTAAACTGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTg                    >  1:18676/1‑141 (MQ=255)
                                                                                                                       aaaaaaGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTgg                   >  2:354939/1‑141 (MQ=255)
                                                                                                                        aaaaaGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTggg                  >  1:697963/1‑141 (MQ=255)
                                                                                                                         aaaaGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTgaagaa                                                                                        <  2:333632/70‑1 (MQ=255)
                                                                                                                         aaaaGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTgaagaa                                                                                        >  1:333632/1‑70 (MQ=255)
                                                                                                                         aaaaGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTGGGt                 >  2:302766/1‑141 (MQ=255)
                                                                                                                           aatggAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTGGGTaa               <  2:86526/138‑1 (MQ=255)
                                                                                                                                aaaTTTTTATAAATTCAAATGTAAAATGCTTGAGCATATAATTATTATTATTTAAGTAAAGAAGAAGAAAGTCATAACTACTGGGATAAACTGAAGCAAATTTCTAAAGTGGAAAGTGTACATGCGCCTAGGGTAATTGaa          <  2:385537/141‑1 (MQ=255)
                                                                                                                                  aTTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGGAATTGGACATGCGCCTTGGGTAATTGaaaa        >  2:70561/1‑141 (MQ=255)
                                                                                                                                    atttttCAATTCAAATGTACAATGCTTAAGCACATAATAATTAATATTTAGGTGAAGAAGACGAACGTCATTACTACTAGGAAAAATTGAAGAAAATTTCTAAAGTGGAAATTTGACATGCGCCTTAGGTAATTGAAAATa      >  2:62105/6‑141 (MQ=255)
                                                                                                                                        aTCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTGGGTAATTGAAAATAGCaa  <  2:672111/141‑1 (MQ=255)
                                                                                                                                        aTCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTGGGTAATTGAAAATAGCaa  <  2:306103/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
ATTTAAAAATATTAAACAAAGTTGTACCTATGAATGACGATGAAAGTTTTAAATTAGGTATTGTATTAAGTTATTTAAAGCAGTATCGTGCATCACAACAATTGTTGTACCCTTTATATAAAAAAGGGAAATTTTTATCAATTCAAATGTACAATGCTTTAGCATATAATTATTATTATTTAGGTGAAGAAGACGAAAGTCATTACTACTGGGATAAATTGAAGCAAATTTCTAAAGTGGAAATTGGACATGCGCCTTGGGTAATTGAAAATAGCAA  >  CP000730/846248‑846524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑