Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	802,515	A→T	7.7%	P262P (CCT→CCA)	dgkA1 ←	possible diacylglycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	802,515	0	A	T	7.7%	209.5 / 4.7	69	P262P (CCT→CCA)	dgkA1	possible diacylglycerol kinase
Reads supporting (aligned to +/- strand):  ref base A (32/31);  new base T (3/3);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTACAAATCATTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAATCAGTCA  >  CP000730/802382‑802654                                                                                                                                      |                                                                                                                                            cTTACAAATCATTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTatat                                                                                                                                      >  1:573298/1‑141 (MQ=255)   tACAAATCATTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGc                                                                                                                                    <  1:430908/141‑1 (MQ=255)    aCAAATCATTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCt                                                                                                                                   <  1:126704/141‑1 (MQ=255)    aCAAATCATTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACAAGGTATATgat                                                                                                                                   >  2:637526/1‑139 (MQ=255)     cAAATCATTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTc                                                                                                                                  >  1:210513/1‑141 (MQ=255)           aTTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATGcc                                                                                                                            >  2:220911/1‑141 (MQ=255)               aCAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTTTTTTTTATATGCTCAATACCTTGa                                                                                                                        >  2:528327/1‑141 (MQ=255)                cAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAGGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAg                                                                                                                       >  2:90032/1‑141 (MQ=255)                cAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAg                                                                                                                       >  2:90026/1‑141 (MQ=255)                   taagtaaCTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTaa                                                                                                                    <  1:621101/141‑1 (MQ=255)                     agtaaCTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAAtt                                                                                                                  >  1:163342/1‑141 (MQ=255)                         aCTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGGATATGCTCAATACCTTGAGTAATTTCAt                                                                                                              >  1:263896/1‑141 (MQ=255)                              aTGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGTTCAATACCTTGAGTAATTTCATTCCaa                                                                                                         <  1:377664/141‑1 (MQ=255)                                ggCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAAtt                                                                                                       >  2:457711/1‑141 (MQ=255)                                 gCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTc                                                                                                      >  1:670288/1‑141 (MQ=255)                                   aTTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCAt                                                                                                    >  1:285811/1‑141 (MQ=255)                                    tttGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATg                                                                                                   >  1:508132/1‑141 (MQ=255)                                    tttGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATg                                                                                                   >  1:153476/1‑141 (MQ=255)                                      tGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCt                                                                                                 >  1:397419/1‑141 (MQ=255)                                              ccTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCt                                                                                         <  1:709060/141‑1 (MQ=255)                                                    tatcaataGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTaa                                                                                   >  1:624392/1‑141 (MQ=255)                                                    tatcaataGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTaa                                                                                   >  1:624353/1‑141 (MQ=255)                                                      tcaataGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTaaaa                                                                                 >  1:224610/1‑141 (MQ=255)                                                        aataGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAat                                                                               <  1:163322/141‑1 (MQ=255)                                                          taGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAatat                                                                             <  2:596119/141‑1 (MQ=255)                                                             gtgtTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCAt                                                                          <  1:392087/141‑1 (MQ=255)                                                                 ttCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTaa                                                                      >  1:78005/1‑141 (MQ=255)                                                                    tAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGGGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAAt                                                                   <  2:364188/141‑1 (MQ=255)                                                                     aaTGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATa                                                                  >  2:507138/1‑141 (MQ=255)                                                                       tGAAATTTCTCCATTTATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTATCAGGTATATGCTCACTAAATTGAGTAATTTCATTCCAATTCACGTTATCTCGATTTTTAAAAATATCATTTAAAATACt                                                                <  2:233226/141‑1 (MQ=255)                                                                       tGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACt                                                                <  2:177232/141‑1 (MQ=255)                                                                       tGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACt                                                                <  1:248864/141‑1 (MQ=255)                                                                        gAAATTTCGCCATCAATATCTACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCTGGTATATGCTCAATACCTTGAGTAATTTCATTCCTATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTg                                                               <  2:590225/141‑1 (MQ=255)                                                                         aaaTTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGa                                                              <  1:529196/141‑1 (MQ=255)                                                                         aaaTTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGa                                                              <  1:20871/141‑1 (MQ=255)                                                                            tttCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAAc                                                           >  2:367608/1‑141 (MQ=255)                                                                             ttCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACt                                                          >  1:483588/1‑141 (MQ=255)                                                                              tCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACtt                                                         >  2:281514/1‑141 (MQ=255)                                                                                  cATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGtt                                                     <  1:162097/141‑1 (MQ=255)                                                                                   aTCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTc                                                    <  2:290430/141‑1 (MQ=255)                                                                                    tCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCTGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCa                                                   >  2:264728/1‑141 (MQ=255)                                                                                    tCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCTGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCa                                                   >  2:264723/1‑141 (MQ=255)                                                                                    tCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCa                                                   <  1:564669/141‑1 (MQ=255)                                                                                     cAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCAt                                                  >  1:337527/1‑141 (MQ=255)                                                                                                 tCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAg                                      >  1:483039/1‑141 (MQ=255)                                                                                                 tCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAg                                      >  1:483101/1‑141 (MQ=255)                                                                                                  cATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAgt                                     <  1:349278/141‑1 (MQ=255)                                                                                                   aTTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCACTTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAAATTTGTTCATTAAAAATGAAAgtg                                    >  2:30687/1‑141 (MQ=255)                                                                                                          gATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAg                                      >  1:294639/1‑132 (MQ=255)                                                                                                          gATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAg                                      <  2:294639/132‑1 (MQ=255)                                                                                                             cTGTTGTAGGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCAc                          <  1:90032/141‑1 (MQ=255)                                                                                                             cTGTTGTAAGTGAAATCTTTTTACCTGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCAc                          <  1:264728/141‑1 (MQ=255)                                                                                                             cTGTTGTAAGTGAAATCTTTTTACCTGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCAc                          <  1:264723/141‑1 (MQ=255)                                                                                                             cTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCAc                          <  1:90026/141‑1 (MQ=255)                                                                                                               gttgtAAGTGAAATCTTTTTACCATGTATATTCTTCATCCCTTTTGAAATGTTATTGCGATGCAGTTTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCa                        <  1:637526/141‑1 (MQ=255)                                                                                                               gttgtAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCa                        <  2:185542/141‑1 (MQ=255)                                                                                                               gttgtAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCa                        <  1:70360/141‑1 (MQ=255)                                                                                                                ttgtAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCAt                       <  2:491318/141‑1 (MQ=255)                                                                                                                      gTGAAAATTTTTTACCAAGGATATGTTTAATACGTTTAGTAATTTAATTCGAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTCAAAATGAACGTGGTCAGTTCACCTTCCTgag                 <  2:263896/141‑1 (MQ=255)                                                                                                                         aaaTCTTTTTACCAGGTATATGCTCAATACCATGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTgaggag              >  1:18722/1‑141 (MQ=255)                                                                                                                             cTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACaa          <  1:220911/141‑1 (MQ=255)                                                                                                                               ttttACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAAt        <  2:483101/141‑1 (MQ=255)                                                                                                                               ttttACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAAt        <  2:483039/141‑1 (MQ=255)                                                                                                                               ttttACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAAt        >  2:164522/1‑141 (MQ=255)                                                                                                                               ttttACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAAt        <  1:655453/141‑1 (MQ=255)                                                                                                                                tttACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAATc       >  2:495303/1‑141 (MQ=255)                                                                                                                                   aCCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAATCAGt    >  1:41223/1‑141 (MQ=255)                                                                                                                                    ccAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAATCAGTc   >  1:169587/1‑141 (MQ=255)                                                                                                                                     cAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAATCAGTCa  <  1:79703/141‑1 (MQ=255)                                                                                                                                      |                                                                                                                                            CTTACAAATCATTGACAGTAAGTAACTGAATGGCATTTGGTATAACCTCAATATCAATAGGTGTTTCTAATGAAATTTCGCCATCAATATCAACTTTCATTGCTGGATCTGTTGTAAGTGAAATCTTTTTACCAGGTATATGCTCAATACCTTGAGTAATTTCATTCCAATTCATGCTATCACGCTTTTTAAAAATATCATTTAAAATACTGAAACTTTGTTCATTAAAAATGAAAGTGTTCAGTTCACCATCTTGAGGAGACAAATCAGTCA  >  CP000730/802382‑802654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑