Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 792,484 A→T 7.0% N504Y (AAC→TAC)  ltaS → possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730792,4840AT 7.0% 181.7 / 4.5 57N504Y (AAC→TAC) ltaSpossible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
Reads supporting (aligned to +/- strand):  ref base A (26/27);  new base T (2/2);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

GACTTGAAGAAAAAAGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACGAAGAACTATTTAATGTTCGGTACTG  >  CP000730/792346‑792622
                                                                                                                                          |                                                                                                                                          
gACTTGAAGAAAAAAGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTacac                                                                                                                                          >  2:357266/1‑141 (MQ=255)
gACTTGAAGAAAAAAGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAAc                                                                                                                                          >  1:147467/1‑141 (MQ=255)
       agaaAAAAGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAAAAAAAAAAAAAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTg                                                                                                                                   <  2:597763/141‑1 (MQ=255)
            aaaGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTc                                                                                                                              >  2:457289/1‑141 (MQ=255)
            aaaGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTc                                                                                                                              >  1:363944/1‑141 (MQ=255)
            aaaGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATAGTAGCTCTGAAGACCATAAGAATGCCATGGAAAAACTATTAGTTGAAAAAATCACACCGGCTAAATGTACAGATGTAAACAGAACTGGGTTc                                                                                                                              >  1:420706/1‑141 (MQ=255)
              aGGATTATATGACAATTCAGTGATTATGATTTATGGTNACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTg                                                                                                                            >  1:604597/1‑141 (MQ=255)
              aGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTg                                                                                                                            >  2:525766/1‑141 (MQ=255)
              aGGATTATATGACAATTCAGTGATTATGATCTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTg                                                                                                                            >  1:268318/1‑141 (MQ=255)
                gATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGa                                                                                                                          <  1:481358/141‑1 (MQ=255)
                 aTTATATGACAATTCCGTGATTATGATTTATGGTGACCAATATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAAAAGAACTGCttcttaata                                                                                                                         >  2:304141/1‑134 (MQ=255)
                   tataTGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTa                                                                                                                       <  1:436955/141‑1 (MQ=255)
                             ttCAGTGATTATGATTTATGGTGACCACTATAGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTgg                                                                                                             <  2:21443/141‑1 (MQ=255)
                                 gTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTATACAGAACTGGTTTCTGGATTAAAATCCCTGGTaaa                                                                                                         >  2:558279/1‑141 (MQ=255)
                                 gTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTATACAGAACTGGTTTCTGGATTAAAATCCCTGGTaaa                                                                                                         >  2:198764/1‑141 (MQ=255)
                                 gTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTaaa                                                                                                         <  1:388539/141‑1 (MQ=255)
                                  tGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAAATGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAAt                                                                                                        <  2:277203/141‑1 (MQ=255)
                                   gATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATc                                                                                                       <  1:498008/141‑1 (MQ=255)
                                             tATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATc                                                                                             <  2:65210/141‑1 (MQ=255)
                                              aTGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCa                                                                                            >  1:554100/1‑141 (MQ=255)
                                                ggTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCaat                                                                                          <  1:181038/141‑1 (MQ=255)
                                                ggTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCaat                                                                                          <  1:26680/141‑1 (MQ=255)
                                                  tGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCaataa                                                                                        >  2:138209/1‑141 (MQ=255)
                                                    atcgatATGGTATCTCTGAAAACAATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACGGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATg                                                                                      <  2:527413/136‑1 (MQ=255)
                                                     ccACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGa                                                                                     <  1:597264/141‑1 (MQ=255)
                                                       aCTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAat                                                                                   <  2:268318/141‑1 (MQ=255)
                                                        cTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAata                                                                                  >  1:274660/1‑141 (MQ=255)
                                                              tATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTgg                                                                            >  2:537438/1‑141 (MQ=255)
                                                              tATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTgg                                                                            <  1:309972/141‑1 (MQ=255)
                                                                  tctGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACAGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCaa                                                                        >  2:648838/1‑141 (MQ=255)
                                                                       aaaCCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGa                                                                   >  1:215306/1‑141 (MQ=255)
                                                                         aCCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATg                                                                 <  2:127550/141‑1 (MQ=255)
                                                                             tAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAAt                                                             <  2:185516/141‑1 (MQ=255)
                                                                             tAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAAt                                                             <  2:544823/141‑1 (MQ=255)
                                                                              aaCAATGCCATGGAAAAACTATTAGGTAAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCacatggatagagtg                                                            >  2:559694/1‑128 (MQ=255)
                                                                                    gCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCcaaca                                                      >  1:282753/1‑141 (MQ=255)
                                                                                      cATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAAt                                                    <  1:636313/141‑1 (MQ=255)
                                                                                        tGGAAAAACTATTAAGAAAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAAttt                                                  <  2:7066/141‑1 (MQ=255)
                                                                                          gAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTa                                                >  1:59994/1‑141 (MQ=255)
                                                                                          gAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGACGATGTAATGCCAACAATTTTa                                                >  1:590732/1‑141 (MQ=255)
                                                                                           aaaaaCTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTAc                                               <  2:59994/141‑1 (MQ=255)
                                                                                           aaaaaCTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTAc                                               <  1:612130/141‑1 (MQ=255)
                                                                                             aaaCTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTATACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACAt                                             <  1:198764/141‑1 (MQ=255)
                                                                                             aaaCTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTATACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACAt                                             <  1:558279/141‑1 (MQ=255)
                                                                                               aCTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACAttt                                           >  2:76322/1‑141 (MQ=255)
                                                                                                     aggcgAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTgg                                     >  1:412310/5‑141 (MQ=255)
                                                                                                           aaaaaTCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGa                               <  1:360899/141‑1 (MQ=255)
                                                                                                           aaaaaTCACAACGTCTAAAATTACAGATTTAAACAGAACTGATTTCTTGATTAAAAGCCCTGGTAAATCTGGTAGTATCAATAATGAATATGCTGGTCAAGGCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGa                               <  2:189297/141‑1 (MQ=255)
                                                                                                                 cacaCCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTAc                                               >  2:396162/1‑119 (MQ=255)
                                                                                                                 cacaCCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTAc                                               <  1:396162/119‑1 (MQ=255)
                                                                                                                   cacCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACgaaga                       <  1:357266/141‑1 (MQ=255)
                                                                                                                        gCTAAATTTACAGATATAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTGAAGTCGATGTAATGCCAACAATTTTACATTTGGGTGGGATAGATACGAAGAACTAt                  >  1:127956/1‑141 (MQ=255)
                                                                                                                         cTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACGAAGAACTAtt                 <  1:607980/141‑1 (MQ=255)
                                                                                                                            aaTTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGGATAGATACGAAGAACTATTTaa              >  1:590347/1‑141 (MQ=255)
                                                                                                                                   aGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACGAAGAACTATTTAATGTTCgg       >  2:641510/1‑141 (MQ=255)
                                                                                                                                      tttAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACGAAGAACTATTTAATGTTCGGTAc    <  2:215306/141‑1 (MQ=255)
                                                                                                                                        tAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACGAAGAACTATTTAATGTTCGGTACTg  >  2:360989/1‑141 (MQ=255)
                                                                                                                                        tAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACGAAGAACTATTTAATGTTCGGTACTg  >  2:395322/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GACTTGAAGAAAAAAGGATTATATGACAATTCAGTGATTATGATTTATGGTGACCACTATGGTATCTCTGAAAACCATAACAATGCCATGGAAAAACTATTAGGTGAAAAAATCACACCGGCTAAATTTACAGATTTAAACAGAACTGGTTTCTGGATTAAAATCCCTGGTAAATCTGGTGGTATCAATAATGAATATGCTGGTCAAGTCGATGTAATGCCAACAATTTTACATTTGGCTGGTATAGATACGAAGAACTATTTAATGTTCGGTACTG  >  CP000730/792346‑792622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑