Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	29,365	A→T	5.7%	A182A (GCA→GCT)	yycI →	hypothetical protein YycI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	29,365	0	A	T	5.7%	223.8 / 4.1	71	A182A (GCA→GCT)	yycI	hypothetical protein YycI
Reads supporting (aligned to +/- strand):  ref base A (31/35);  new base T (2/2);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

CTGTAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACGAATGTTCAATT  >  CP000730/29226‑29502                                                                                                                                            |                                                                                                                                          cTGTAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAg                                                                                                                                          >  1:312479/1‑141 (MQ=255)  tGTAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGa                                                                                                                                         >  1:39450/1‑141 (MQ=255)  tGTAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGa                                                                                                                                         >  2:253201/1‑141 (MQ=255)    tAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATa                                                                                                                                       >  2:254544/1‑141 (MQ=255)    tAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATa                                                                                                                                       <  2:382128/141‑1 (MQ=255)    tAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATa                                                                                                                                       <  2:426858/141‑1 (MQ=255)    cagaaTCTGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATa                                                                                                                                       <  2:536125/138‑1 (MQ=255)       aaTATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAAAAATCAAAGATGGATGACATTAAGCCCACAGATGGTGCAGATaaga                                                                                                                                    <  2:348667/141‑1 (MQ=255)        atatGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATaagaa                                                                                                                                   >  2:264510/1‑141 (MQ=255)        atatGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATaagaa                                                                                                                                   >  2:190181/1‑141 (MQ=255)            gAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCAt                                                                                                                               <  2:277694/141‑1 (MQ=255)             aaCAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATc                                                                                                                              >  2:354878/1‑141 (MQ=255)               cAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATCACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCaa                                                                                                                            >  1:493050/1‑141 (MQ=255)                aaaCGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAg                                                                                                                           <  1:253035/141‑1 (MQ=255)                 aaCGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGt                                                                                                                          <  1:166289/141‑1 (MQ=255)                 aaCGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGt                                                                                                                          >  1:374081/1‑141 (MQ=255)                 aaCGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGt                                                                                                                          >  1:374082/1‑141 (MQ=255)                   cGTATGATGCTTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGa                                                                                                                        <  2:457429/141‑1 (MQ=255)                   cGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGa                                                                                                                        >  1:335767/1‑141 (MQ=255)                       tgatgtTTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTgg                                                                                                                    <  2:260755/141‑1 (MQ=255)                            atTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTga                                                                                                               <  2:494448/141‑1 (MQ=255)                               ttCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGaa                                                                                                            <  1:668013/141‑1 (MQ=255)                                             aaCAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCa                                                                                              >  2:322891/1‑141 (MQ=255)                                                 gTAAAGCGATGTTGAACTTTCACATAGAACATAACAAAGCCACCAGTTATAAACAATCAATGATGGAAGCAATTAAGACCACAGATGGTGCAGATAAGAAGCATCACGCGATTGGAGTGCGAAAAGCacgcttctccatga                                                                                          >  2:384823/1‑128 (MQ=255)                                                    gagagATGTTGAACTTTAATATAGACAATAACTAAGCGACTAGTTATAAACAATCCAAGATGGATGACATTAAGCCCACAGATTGTGACGATAAGAACAATAACGTGATTGGTGTGAGAACAGCCATCGAGGCattataat                                                                                       <  2:171916/137‑3 (MQ=255)                                                        gATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATaa                                                                                   <  2:590240/141‑1 (MQ=255)                                                         aTGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAAt                                                                                  >  2:122956/1‑141 (MQ=255)                                                         aTGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAAt                                                                                  >  1:264461/1‑141 (MQ=255)                                                           gTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCg                                                                                >  1:694079/1‑141 (MQ=255)                                                                attgcaaTATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACt                                                                           <  1:527964/136‑1 (MQ=255)                                                                          agaagaTAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAgg                                                                 <  1:365264/141‑1 (MQ=255)                                                                                tAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCACTCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGtgatga                                                           >  1:134326/1‑141 (MQ=255)                                                                                  aCAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAg                                                         >  2:425877/1‑141 (MQ=255)                                                                                   cAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGt                                                        <  1:60461/141‑1 (MQ=255)                                                                                   cAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGt                                                        <  1:60430/141‑1 (MQ=255)                                                                                    aaaGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTc                                                       <  2:606719/141‑1 (MQ=255)                                                                                    aaaGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTc                                                       <  1:472510/141‑1 (MQ=255)                                                                                     aaGCGAATAGTTATAAAAAATAAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAAAAAAAAAAAAAAAAAAGGTGATGAAGTCa                                                      <  2:279744/141‑1 (MQ=255)                                                                                         gACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTaa                                                  <  1:354878/141‑1 (MQ=255)                                                                                         gACTAGTAAAAAACAAGCAATGATGGATGACATTAAGCCCACAGATGGAGCAGATAAGAAGCATCAAGTGATTGGTGTAAAAAAAGCAATCGAGGCATTATATTATAAAAAAAAAAAAAAAAAAGGTGATGAAGTCATTaa                                                  <  2:222185/141‑1 (MQ=255)                                                                                          aCTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAACAGGTGATGAAGACATTCAt                                                 >  2:497277/1‑141 (MQ=255)                                                                                            tAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGCTAAGAAGCATCAAGTGATTGGCGTGCGAAAAGCAATAGAGGCATTATATTATAATAGTTACTTGATAAAAGGTGATGATGTCATAAATgt                                               >  2:60907/1‑140 (MQ=255)                                                                                            tAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGc                                               >  1:269120/1‑141 (MQ=255)                                                                                            aagtaaaaaaCAATCAATGATGGATGACATTAAGCCGACAGATGGTGAAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAAACGTTAATTGAAAAAAGGTGATGAAGTCATTAATGc                                               <  2:404934/134‑1 (MQ=255)                                                                                                tataAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGa                                           >  2:158769/1‑141 (MQ=255)                                                                                                tataAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGa                                           <  2:32078/141‑1 (MQ=255)                                                                                                tataAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGa                                           <  2:664931/141‑1 (MQ=255)                                                                                                tataAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTAGAAAAAAGGTGATGAAGTCATTAATGCTAGa                                           <  2:196770/141‑1 (MQ=255)                                                                                                 ataAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGAc                                          <  1:142430/141‑1 (MQ=255)                                                                                                 ataAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGAc                                          <  1:142556/141‑1 (MQ=255)                                                                                                    aaCAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCg                                       >  1:186143/1‑141 (MQ=255)                                                                                                    aaCAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCg                                       <  2:383810/141‑1 (MQ=255)                                                                                                           aatGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTtacta                                <  1:195476/141‑1 (MQ=255)                                                                                                            atgatgGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTtactac                               <  2:586782/141‑1 (MQ=255)                                                                                                            atgatgGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTtactac                               >  1:684971/1‑141 (MQ=255)                                                                                                             tgatgGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTactact                              <  2:684420/141‑1 (MQ=255)                                                                                                             tgatgGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTactact                              <  1:195553/141‑1 (MQ=255)                                                                                                              gatggatgACATTAAGCCCACAGATGGTGCTGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAgg                                                                                                <  1:450193/74‑1 (MQ=255)                                                                                                              gatggatgACATTAAGCCCACAGATGGTGCTGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAgg                                                                                                >  2:450193/1‑74 (MQ=255)                                                                                                              gatggatgACATTAAGCCCACAGATGGTGCTGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAgg                                                                                                >  2:450196/1‑74 (MQ=255)                                                                                                              gatggatgACATTAAGCCCACAGATGGTGCTGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAgg                                                                                                <  1:450196/74‑1 (MQ=255)                                                                                                               atggatgACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCa                            <  1:260742/141‑1 (MQ=255)                                                                                                                     gACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGtg                      >  2:130249/1‑141 (MQ=255)                                                                                                                     gACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGtg                      >  2:130192/1‑141 (MQ=255)                                                                                                                            aGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGaaa               >  1:652878/1‑141 (MQ=255)                                                                                                                              cccACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACg             >  1:473502/1‑141 (MQ=255)                                                                                                                                cacaGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACGaa           <  1:164433/141‑1 (MQ=255)                                                                                                                                cacaGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACGaa           >  1:623801/1‑141 (MQ=255)                                                                                                                                     aTGGTGTAGATAAGAAGCATCAAGTGATTGGTGTGAGAATAGTAATCTAGGCATTATATTATAATAATTAAAAGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACGAATGTTc      <  2:398307/141‑1 (MQ=255)                                                                                                                                      tGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACGAATGTTCa     >  1:552526/1‑141 (MQ=255)                                                                                                                                         tGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACGAATGTTCAAtt  >  2:288447/1‑141 (MQ=255)                                                                                                                                            |                                                                                                                                          CTGTAAAATATGAACAAACGTATGATGATTTTCCGATTTTAAATAACAGTAAAGCGATGTTGAACTTTAATATAGAAGATAACAAAGCGACTAGTTATAAACAATCAATGATGGATGACATTAAGCCCACAGATGGTGCAGATAAGAAGCATCAAGTGATTGGTGTGAGAAAAGCAATCGAGGCATTATATTATAATCGTTACTTGAAAAAAGGTGATGAAGTCATTAATGCTAGACTCGGTTACTACTCAGTCGTGAATGAAACGAATGTTCAATT  >  CP000730/29226‑29502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑