Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 111,041 C→T 5.2% G37G (GGC→GGT USA300HOU_0110 → possible staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730111,0410CT 5.2% 399.5 / 5.1 132G37G (GGC→GGTUSA300HOU_0110possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (58/64);  new base T (2/6);  total (60/72)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

GGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATGGATTGT  >  CP000730/110901‑111181
                                                                                                                                            |                                                                                                                                            
ggAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGc                                                                                                                                              >  1:364628/1‑141 (MQ=255)
ggAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGc                                                                                                                                              >  2:322256/1‑141 (MQ=255)
 gagaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCa                                                                                                                                             >  2:658675/1‑141 (MQ=255)
  agaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCAt                                                                                                                                            >  1:266016/1‑141 (MQ=255)
  agaTGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGAGAAAACGTGTAAATAAATTAGTGCGTGGTATTAGTCTTCTGTTTTTAGACATTAGTATCACTGCTGGTTGTGGCAt                                                                                                                                            >  1:530688/1‑141 (MQ=255)
       tataATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTa                                                                                                                                       <  1:204791/141‑1 (MQ=255)
       tataATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTa                                                                                                                                       <  2:460456/141‑1 (MQ=255)
              aTGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGc                                                                                                                                <  1:123866/141‑1 (MQ=255)
              aTGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGc                                                                                                                                <  2:365608/141‑1 (MQ=255)
               tGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCTTTTGTAAAGAAGCg                                                                                                                               <  1:522117/141‑1 (MQ=255)
                 gCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGa                                                                                                                             >  1:29968/1‑141 (MQ=255)
                    cAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAAt                                                                                                                          >  1:278513/1‑141 (MQ=255)
                      aTCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATaa                                                                                                                        <  2:620145/141‑1 (MQ=255)
                        cAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAg                                                                                                                      <  2:274989/141‑1 (MQ=255)
                        cAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAAGGATAAAACGTGGAAATAAATTAGTGCTTGGTATTAGTCGTCTGATTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAg                                                                                                                      <  1:566324/141‑1 (MQ=255)
                         aGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGa                                                                                                                     >  1:571062/1‑141 (MQ=255)
                         aGAATATCAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGa                                                                                                                     <  1:641395/141‑1 (MQ=255)
                          gAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGaa                                                                                                                    >  2:160878/1‑141 (MQ=255)
                          gAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGaa                                                                                                                    <  1:433472/141‑1 (MQ=255)
                           aaTATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGaaa                                                                                                                   >  1:202248/1‑141 (MQ=255)
                           aaTATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGaaa                                                                                                                   <  1:322256/141‑1 (MQ=255)
                               tGAAATCAATCCAGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGtt                                                                                                               <  2:627413/141‑1 (MQ=255)
                                    tcaatcCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGaa                                                                                                          >  1:267257/1‑141 (MQ=255)
                                     caatcCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGaaa                                                                                                         <  1:11065/141‑1 (MQ=255)
                                     caatcCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGATATGaaa                                                                                                         <  2:516590/141‑1 (MQ=255)
                                      aatcCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGaaaa                                                                                                        <  1:618611/141‑1 (MQ=255)
                                      aagcccGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTATTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCTGAAATAAAGAAAAGttttttcaa                                                                                                        <  2:384357/138‑6 (MQ=255)
                                       atcCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGaaaaa                                                                                                       <  1:434940/141‑1 (MQ=255)
                                       atcCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGaaaaa                                                                                                       <  1:229117/141‑1 (MQ=255)
                                         cccGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAAc                                                                                                     >  1:251011/1‑141 (MQ=255)
                                           cGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACAt                                                                                                   <  2:360714/141‑1 (MQ=255)
                                             gAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTg                                                                                                 >  2:618174/1‑141 (MQ=255)
                                                 aaGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAgta                                                                                             >  1:308146/1‑141 (MQ=255)
                                                  agagagTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGTATGGGTAAAGAAGCGGAAATAAAg                                                                                                                      >  2:688131/1‑115 (MQ=255)
                                                  agagagTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGTATGGGTAAAGAAGCGGAAATAAAg                                                                                                                      <  1:688215/115‑1 (MQ=255)
                                                  agagagTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGTATGGGTAAAGAAGCGGAAATAAAg                                                                                                                      <  1:688131/115‑1 (MQ=255)
                                                  agagagTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGTATGGGTAAAGAAGCGGAAATAAAg                                                                                                                      >  2:688215/1‑115 (MQ=255)
                                                    agagTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAgtatgt                                                                                          >  2:215784/1‑141 (MQ=255)
                                                     gagTGATGAAATGATAAAAAGTATAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAgtatgta                                                                                         >  2:174151/1‑141 (MQ=255)
                                                      agTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAgtatgtat                                                                                        <  2:444886/141‑1 (MQ=255)
                                                         gattaAATGATAAAACGTGTAAATAAATTAGTGCTTGCTATTAGTCTTATGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGATAAAGAAGCCGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCg                                                                                     <  2:101885/141‑1 (MQ=18)
                                                           tgaAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGAt                                                                                   <  1:367352/141‑1 (MQ=255)
                                                           tgaAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGAt                                                                                   <  1:292745/141‑1 (MQ=255)
                                                           tgaAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGAt                                                                                   <  1:367513/141‑1 (MQ=255)
                                                            gaAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCTCTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGAtt                                                                                  <  1:641496/141‑1 (MQ=255)
                                                              aataaaaaaagaggtAAATAAATTATTACTTGTAATTAGTATTATGTTTTTATTAACTAGTAACAAAGCTTGTTGTTGCATGAATAAAGAAGAGTAAATAAAAAAAAGAAATAAAAAAACATTGAGTATGTATCCGATTaa                                                                                <  2:642201/128‑1 (MQ=9)
                                                              aaagATAAAACGTGTAAATAAATTAGTACTTGGTATTAGTCTTCTGTTTTTAATCTTTAGTATCACTGCTGGTAGTGGAATGGGTAAAGCCGCGGAAATAAAGAAAAAAAAAAAAAAAACATTGAGTATGTATCCGATTaa                                                                                <  2:40112/138‑1 (MQ=18)
                                                              aaagATAAAACGTGTAAATAAATTAATGCTTGGTATTAGTCTGGTGTTTTTAGTCATTAGTATCAATGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAAAAAAAAAAAAAAAAAACATTGAGTATGTATCCGATTaa                                                                                <  2:49919/138‑1 (MQ=18)
                                                                tGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTaaaa                                                                              >  2:59599/1‑141 (MQ=255)
                                                                 gATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTaaaaa                                                                             >  1:191347/1‑141 (MQ=255)
                                                                    aaaaCGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGttttttcaa                                                                                                        >  2:689806/1‑106 (MQ=255)
                                                                    aaaaCGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGttttttcaa                                                                                                        <  1:689806/111‑6 (MQ=255)
                                                                     aaaCGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATaa                                                                                                                        <  2:307892/94‑1 (MQ=255)
                                                                     aaaCGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATaa                                                                                                                        >  1:307892/1‑94 (MQ=255)
                                                                     aaaCGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTa                                                                         >  1:295659/1‑141 (MQ=255)
                                                                             aaataaatTAGTGCTTGGTATTAGTCTTCTGCTTTTAGTCATTAGGATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGAttt                                                                 >  2:682187/1‑141 (MQ=255)
                                                                              aataaatTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTta                                                                >  1:172451/1‑141 (MQ=255)
                                                                                 aaatTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATAc                                                             <  2:7966/141‑1 (MQ=255)
                                                                                 aaatTAGTGATTAGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAATATAAAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATAc                                                             <  2:455902/141‑1 (MQ=17)
                                                                                  aatTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACg                                                            <  1:160878/141‑1 (MQ=255)
                                                                                  aatTAGTGCTTGGTATTAGTCTGCTGTTTTTAGTCATTAGTATCAATGCAGGTTGTGTTATGGGTAAAGAAGCGGAAATAAAAAAAAAAAAAAAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACg                                                            <  2:698385/141‑1 (MQ=17)
                                                                                     tAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATa                                                         >  2:65620/1‑141 (MQ=255)
                                                                                        tGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATACGATTAAAAATCTAGAGGATTTATATGATTaag                                                      >  2:564989/1‑141 (MQ=255)
                                                                                         gCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAaaga                                                     >  2:74553/1‑141 (MQ=255)
                                                                                           ttGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAaagaag                                                   >  2:382119/1‑141 (MQ=255)
                                                                                             ggTATTAGTCTGCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGTATGGGTAAAGAAGCGGAAATAAAAAAAAAAAAAAAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGa                                                 <  2:152217/141‑1 (MQ=17)
                                                                                               tactAGTCTTCTGTTTTTAGTCATTAGTATCACTACTGGTTGTTGCATGGGTAAAGAAGCGGAGATAAAGAAAAGTCGTGAAAAAACATAAAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGaca                                               >  2:346211/4‑139 (MQ=14)
                                                                                               tATTAGTTTTCTGTTTTTAGTCATTAGTATCACTGGTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGata                                               <  2:266246/141‑1 (MQ=255)
                                                                                                    gTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGtg                                          <  1:478351/141‑1 (MQ=255)
                                                                                                     tcttctGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGtga                                         <  1:200111/141‑1 (MQ=255)
                                                                                                     tcttatGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGtga                                         <  2:37711/141‑1 (MQ=255)
                                                                                                        tctGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGtgatga                                      <  1:618174/141‑1 (MQ=255)
                                                                                                          tGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATc                                    >  2:28945/1‑141 (MQ=255)
                                                                                                          tGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATc                                    >  1:60393/1‑141 (MQ=255)
                                                                                                          tGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGATTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATc                                    <  2:33775/141‑1 (MQ=255)
                                                                                                             ttttAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAAt                                 <  1:527520/141‑1 (MQ=255)
                                                                                                              tttAGTCTTTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAAtt                                >  2:128679/1‑141 (MQ=255)
                                                                                                              tttAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAAtt                                <  1:621765/141‑1 (MQ=255)
                                                                                                              tttAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAAtt                                <  1:85356/141‑1 (MQ=255)
                                                                                                              tttAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAAtt                                <  1:145097/141‑1 (MQ=255)
                                                                                                               ttAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAAttt                               <  1:264949/141‑1 (MQ=255)
                                                                                                                tAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTg                              >  2:192835/1‑141 (MQ=255)
                                                                                                                 aGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGa                             >  1:238169/1‑141 (MQ=255)
                                                                                                                     aTTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATaaa                         >  2:515136/1‑141 (MQ=255)
                                                                                                                       tAGTATCATTGATAGTTGAGGAATGGGTAAAGAAGCTGAAATAAAGAAAAAAAAAAAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATaaaaa                       <  2:423122/141‑1 (MQ=17)
                                                                                                                       tAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATaaaaa                       >  1:269799/1‑141 (MQ=255)
                                                                                                                            tcaatgctgGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATAc                                                             <  2:126253/94‑1 (MQ=255)
                                                                                                                            tcaatgctgGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATAc                                                             >  1:126253/5‑98 (MQ=255)
                                                                                                                              aCTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAg                >  2:612681/1‑141 (MQ=255)
                                                                                                                               ctgctgGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATCAAAATGCTAAgg               >  2:311468/1‑141 (MQ=255)
                                                                                                                               ctgctgGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAgg               >  2:59808/1‑141 (MQ=255)
                                                                                                                                  ctgGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGaa            >  1:328431/1‑141 (MQ=255)
                                                                                                                                   tgGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAAc           >  2:87498/1‑141 (MQ=255)
                                                                                                                                     gTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACAt         <  1:389710/141‑1 (MQ=255)
                                                                                                                                       tgtgGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATgg       >  1:166210/1‑141 (MQ=255)
                                                                                                                                         tgGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATGGAt     >  2:205173/1‑141 (MQ=255)
                                                                                                                                         tgGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATGGAt     >  2:58366/1‑141 (MQ=255)
                                                                                                                                           gCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATGGATTg   <  1:174151/141‑1 (MQ=255)
                                                                                                                                            cATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATGGATTGt  >  2:235491/1‑141 (MQ=255)
                                                                                                                                            cATGGGGAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATGGATTGt  <  2:96414/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GGAGATGTATAATCATGGCTCAATCAGAATATGAAATCAATCCCGGAAAAAGAGAGTGATGAAATGATAAAACGTGTAAATAAATTAGTGCTTGGTATTAGTCTTCTGTTTTTAGTCATTAGTATCACTGCTGGTTGTGGCATGGGTAAAGAAGCGGAAATAAAGAAAAGTTTTGAAAAAACATTGAGTATGTATCCGATTAAAAATCTAGAGGATTTATACGATAAAGAAGGATATCGTGATGATCAATTTGATAAAAATGATAAGGGAACATGGATTGT  >  CP000730/110901‑111181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: