Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,863,480 G→T 9.3% L36L (CTC→CTA)  USA300HOU_2707 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,863,4800GT 9.3% 227.7 / 5.0 75L36L (CTC→CTA) USA300HOU_2707hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/35);  new base T (6/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGTAATATTAAAAAAACGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAGGTTAGCATCG  >  CP000730/2863341‑2863619
                                                                                                                                           |                                                                                                                                           
tAGTAATATTAAAAAAACGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTga                                                                                                                                            <  2:248025/141‑1 (MQ=255)
   tAATATTAAAAAAACGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGaa                                                                                                                                         <  1:388678/141‑1 (MQ=255)
   tAATATTAAAAAAACGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACA‑TACGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAAc                                                                                                                                        <  2:52169/141‑1 (MQ=255)
      tatTAAAAAAACGACCAATTCTTGTTTTTTCTCTATATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACAt                                                                                                                                      >  2:96961/1‑141 (MQ=255)
            aaaaaCGACCAATTCTTGTTTTTTTTTTATATTTAACAGGATATAAGTATTCAGACAAAAATGTAAATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAAAAATTTGCTTTTTATTGAGAACATCAatat                                                                                                                                >  2:532844/1‑141 (MQ=255)
            aaaaaCGACCAATTCTTGTTTTTTTTTTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGATATTTGCTTTTTATTGAGAACATCAatat                                                                                                                                >  2:532917/1‑141 (MQ=255)
             aaaaCGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATc                                                                                                                               >  1:107114/1‑141 (MQ=255)
              aaaCGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCt                                                                                                                              <  1:533300/141‑1 (MQ=255)
                 cGACCAATTCTTGTTTTTTTTTTTTTTTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATTTAACATTAATAATATTAGAAATTTGCTTTTTTTTTATTACTTTTATATCTaaa                                                                                                                           >  2:233675/1‑140 (MQ=255)
                 cGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGAGATACGCATTCAGAAGAGAACGTACACACCGTCTTTGATTGGTTATCAAATATAACATTAATAATATAATCAATTTGCTTTGTATTCAGGAcgccaagacccgac                                                                                                                           >  2:236082/1‑128 (MQ=255)
                  gACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGc                                                                                                                          >  2:309671/1‑141 (MQ=255)
                    ccAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGt                                                                                                                        >  1:329091/1‑141 (MQ=255)
                           ttGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTAt                                                                                                                 >  2:378023/1‑141 (MQ=255)
                           ttGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAAGGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTAt                                                                                                                 >  1:591662/1‑141 (MQ=255)
                                       tAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTg                                                                                                     <  1:458973/141‑1 (MQ=255)
                                         aaTTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCt                                                                                                   <  2:107114/141‑1 (MQ=255)
                                         aaTTTAACAGGATATAAAAAAAAAAACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCt                                                                                                   <  2:332285/141‑1 (MQ=255)
                                         aaTTTAAAAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCt                                                                                                   <  1:606366/141‑1 (MQ=255)
                                            ttAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAg                                                                                                        >  2:39018/1‑133 (MQ=255)
                                            ttAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAg                                                                                                        <  1:39018/133‑1 (MQ=255)
                                            ttAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGa                                                                                                >  2:275807/1‑141 (MQ=255)
                                              aaCAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTTAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGaaa                                                                                              >  2:502504/1‑141 (MQ=255)
                                              aaCAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGaaa                                                                                              >  1:535612/1‑141 (MQ=255)
                                                  ggATATAAGTATTCAGACAAAACTGTACATTTCGTCTTTGATTCGTTCTTACATATAACATTAATAATATTAGCAATTAGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAg                                                                                          <  1:242440/141‑1 (MQ=255)
                                                  ggATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAg                                                                                          <  1:267884/141‑1 (MQ=255)
                                                         aGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTTAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAAttt                                                                                   >  2:346243/1‑141 (MQ=255)
                                                          gTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGCATTTc                                                                                  <  2:336412/141‑1 (MQ=255)
                                                          gTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTc                                                                                  <  2:444072/141‑1 (MQ=255)
                                                            aTTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTATTTATTGAGAACATCAACATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTAAt                                                                                >  2:609895/1‑141 (MQ=255)
                                                               cAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGtt                                                                             >  2:252046/1‑141 (MQ=255)
                                                                 gACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTTTTTTTTTTTTCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCt                                                                           >  2:392202/1‑141 (MQ=255)
                                                                 gACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCt                                                                           <  1:468642/141‑1 (MQ=255)
                                                                 gACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCt                                                                           <  1:468649/141‑1 (MQ=255)
                                                                 aaCAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCt                                                                           <  2:298058/140‑1 (MQ=255)
                                                                 aaCAAAAATCTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACAAAAATATCTAAGCGTATCCTATATTTCTCACGTGCTTGAAAATAGGGAATTTCGAGTTcg                                                                           <  2:82180/140‑2 (MQ=255)
                                                                    aaaaaTGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTaaa                                                                        <  2:673853/141‑1 (MQ=255)
                                                                      aaaTGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATc                                                                      >  2:336712/1‑141 (MQ=255)
                                                                        aTGTACATTTCGTCTTTGATTGTTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCaa                                                                    <  2:414306/141‑1 (MQ=255)
                                                                          gTACATTTCGTCTTTGATTGGTTATTAAATATAACATTGATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCaaaa                                                                  <  1:84659/141‑1 (MQ=255)
                                                                          gTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCaaaa                                                                  >  1:194720/1‑141 (MQ=255)
                                                                             cATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGt                                                               >  2:697835/1‑141 (MQ=255)
                                                                               tttCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTAt                                                             >  2:370818/1‑141 (MQ=255)
                                                                               tttCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTAt                                                             >  1:143350/1‑141 (MQ=255)
                                                                                  cGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATttg                                                          <  2:111114/141‑1 (MQ=255)
                                                                                     cTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATttgttg                                                       <  2:181450/141‑1 (MQ=255)
                                                                                       ttGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTTATAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTtgtg                                                     <  2:224534/141‑1 (MQ=255)
                                                                                       ttGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTtgtg                                                     >  1:455593/1‑141 (MQ=255)
                                                                                        tGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTTTTTTTTTTTTCTATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTTTTtgttgttt                                                    >  2:94040/1‑139 (MQ=255)
                                                                                          aTTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGttt                                                  >  1:556727/1‑141 (MQ=255)
                                                                                            tGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAATTGTATTTGTTGTGTTTCa                                                >  2:160645/1‑141 (MQ=255)
                                                                                             ggTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTTAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCaa                                               <  1:502504/141‑1 (MQ=255)
                                                                                                tattaAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGt                                            <  1:97411/141‑1 (MQ=255)
                                                                                                   taAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTtaa                                         >  2:12818/1‑141 (MQ=255)
                                                                                                    aaaTATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTtaat                                        >  2:99486/1‑141 (MQ=255)
                                                                                                          aaCATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCAt                                  <  1:550535/141‑1 (MQ=255)
                                                                                                          aaCATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCAt                                  >  1:179942/1‑141 (MQ=255)
                                                                                                                aataatATTAGAAATTTGCTTTTTTTTTTTTTTTTTAATATCTAAGCGTATCCTATTTTTCTCTGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATTTATTTGTTGTTTTTCAAGGTTAATAATCATTTTatt                            >  2:375503/1‑139 (MQ=255)
                                                                                                                aataatATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTTTTTTTTTTTAAAATAGGGAATTTCATGTTCTAAATCAGAATGTATTTGTTGTGTTTCAAGGTTAATCATCATTTTAAt                            >  2:375540/1‑141 (MQ=255)
                                                                                                                  taataTTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAAtct                          <  1:119787/141‑1 (MQ=255)
                                                                                                                       ttAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAAt                     <  2:226585/141‑1 (MQ=255)
                                                                                                                       ttAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAAt                     >  1:232482/1‑141 (MQ=255)
                                                                                                                        tAGAAAGTTGATTGTTATTGAGAACATCAATATCTAAGCGTATCCTGTTTTTCTTAGGTGCTTGAAAATAGGGAATTTCATGTTCTTCATCAAAATGTTTTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAAtt                    <  2:119839/141‑1 (MQ=255)
                                                                                                                         aGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTTAAGGTTAATAATCATTTTAATCTCCAATTc                   >  2:493759/1‑141 (MQ=255)
                                                                                                                           aaaTTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCtt                 <  2:535612/141‑1 (MQ=255)
                                                                                                                           aaaTTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCtt                 <  1:693907/141‑1 (MQ=255)
                                                                                                                             aTTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCtttt               >  2:481159/1‑141 (MQ=255)
                                                                                                                             aTTTGCTTGTTATTGAGAACATCAATATCTAAGCGTGTCCTATTTTTCTCAGTTGCGTGAAATTAGGGAATTTCATGTTCTAAATCAAAATTTATTTTTTGTGTTTCAATGTTAATAATCATTTTAATCTCCACTTCtttt               >  2:323196/1‑141 (MQ=255)
                                                                                                                                tGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAATGTTAATAATCATTTTAATATCCAATTCTTTTTAg            >  2:648521/1‑141 (MQ=255)
                                                                                                                                tGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAg            <  2:550817/141‑1 (MQ=255)
                                                                                                                                 gCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAgg           >  2:464987/1‑141 (MQ=255)
                                                                                                                                  cTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAGGt          <  1:655132/141‑1 (MQ=255)
                                                                                                                                    ttttATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAGGTTa        <  1:646028/141‑1 (MQ=255)
                                                                                                                                    ttttATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAGGTTa        <  1:442007/141‑1 (MQ=255)
                                                                                                                                      ttATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAGGTTAGc      <  1:275807/141‑1 (MQ=255)
                                                                                                                                        aTTGAGAACATCAATATATAAGCGTATCCTATTTTTCACAGGTGATTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCTAATTCTTTTTAGGTTAGCAt    <  2:78918/141‑1 (MQ=255)
                                                                                                                                          tGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAGGTTAGCATCg  <  2:583267/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TAGTAATATTAAAAAAACGACCAATTCTTGTTTTTTCTCTAAATTTAACAGGATATAAGTATTCAGACAAAAATGTACATTTCGTCTTTGATTGGTTATTAAATATAACATTAATAATATTAGAAATTTGCTTTTTATTGAGAACATCAATATCTAAGCGTATCCTATTTTTCTCAGGTGCTTGAAAATAGGGAATTTCATGTTCTAAATCAAAATGTATTTGTTGTGTTTCAAGGTTAATAATCATTTTAATCTCCAATTCTTTTTAGGTTAGCATCG  >  CP000730/2863341‑2863619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑