Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,769,004 A→G 7.2% V35A (GTT→GCT)  gpo ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,769,0040AG 7.2% 161.3 / 4.0 57V35A (GTT→GCT) gpoABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (23/29);  new base G (2/2);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

ACAATGCTTTACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTGTTAGTTCTATATTAA  >  CP000730/2768867‑2769143
                                                                                                                                         |                                                                                                                                           
aCAATGCTTTACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGcaacaa                                                                                                                                          >  1:618884/1‑141 (MQ=255)
  aaTGCTTTACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAAt                                                                                                                                        <  2:44297/141‑1 (MQ=255)
   aTGCTTTACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAAtt                                                                                                                                       <  1:190504/141‑1 (MQ=255)
      cTTTACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCAc                                                                                                                                    >  2:238164/1‑141 (MQ=255)
       tttACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCAcc                                                                                                                                   <  2:649470/141‑1 (MQ=255)
       tttACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCAcc                                                                                                                                   <  1:223167/141‑1 (MQ=255)
        ttACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGATCCAGACTCACCCATAATTGCAAAAAATTCAACa                                                                                                                                  >  2:393217/1‑141 (MQ=255)
         tACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAg                                                                                                                                 >  2:195548/1‑141 (MQ=255)
           atttgtattttCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCt                                                                                                                               <  2:504406/134‑1 (MQ=255)
                aTTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAAc                                                                                                                          >  1:31752/1‑141 (MQ=255)
                    ttCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGAt                                                                                                                      <  2:48645/141‑1 (MQ=255)
                     tCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATa                                                                                                                     >  1:683418/1‑141 (MQ=255)
                     tCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATa                                                                                                                     <  1:681523/141‑1 (MQ=255)
                                  aTGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGAtt                                                                                                        >  2:49374/1‑141 (MQ=255)
                                    gtgCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTaa                                                                                                      >  2:295075/1‑141 (MQ=255)
                                     tgCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAg                                                                                                     <  2:482971/141‑1 (MQ=255)
                                      gcgcCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGCAGCAATTAACTCTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAACTTCATTTGATTAAGt                                                                                                    >  2:676492/1‑141 (MQ=255)
                                      gcgcCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTAACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGt                                                                                                    >  2:676611/1‑141 (MQ=255)
                                       cgcCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTg                                                                                                   >  2:555818/1‑141 (MQ=255)
                                           aTCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGt                                                                                               <  1:350169/141‑1 (MQ=255)
                                           aTCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGt                                                                                               <  2:305676/141‑1 (MQ=255)
                                            tCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTc                                                                                              <  2:445782/141‑1 (MQ=255)
                                               acaATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTa                                                                                           <  1:679081/141‑1 (MQ=255)
                                                  ataatGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCa                                                                                        <  2:97989/141‑1 (MQ=255)
                                                     atGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTc                                                                                     <  1:181467/141‑1 (MQ=255)
                                                                   tAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATa                                                                       >  2:169543/1‑141 (MQ=255)
                                                                   tAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATa                                                                       >  1:336411/1‑141 (MQ=255)
                                                                    aGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATaa                                                                      >  1:495937/1‑141 (MQ=255)
                                                                     gTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATaaa                                                                     <  1:324168/141‑1 (MQ=255)
                                                                      tCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAAc                                                                    >  2:707206/1‑141 (MQ=255)
                                                                      tCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAAc                                                                    <  1:144256/141‑1 (MQ=255)
                                                                          tCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCttt                                                                >  1:446549/1‑141 (MQ=255)
                                                                          tCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCttt                                                                >  1:90228/1‑141 (MQ=255)
                                                                          tCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCttt                                                                >  1:28165/1‑141 (MQ=255)
                                                                           cAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCtttt                                                               >  2:401533/1‑141 (MQ=255)
                                                                              aagaagCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGAtt                                                                                                        >  1:228530/1‑97 (MQ=255)
                                                                              aagaagCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGAtt                                                                                                        <  2:228530/97‑1 (MQ=255)
                                                                              aagaagCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTa                                                            <  2:449852/141‑1 (MQ=255)
                                                                               agaagCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTAc                                                           >  1:517542/1‑141 (MQ=255)
                                                                                   gCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGt                                                       >  2:313947/1‑141 (MQ=255)
                                                                                   gCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGt                                                       <  1:96866/141‑1 (MQ=255)
                                                                                    cAATTAAGTTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGtt                                                      <  2:172887/141‑1 (MQ=255)
                                                                                    cAATTAAATTTAGTAGTGTAGACTTCCCTGCCCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGtt                                                      <  1:709844/141‑1 (MQ=255)
                                                                                       ttAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCa                                                   >  2:193101/1‑141 (MQ=255)
                                                                                            tttAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCt                                              <  2:151540/141‑1 (MQ=255)
                                                                                                gtagtGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAGCAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCtt                                                                 >  2:122345/1‑118 (MQ=255)
                                                                                                gtagtGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAGCAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCtt                                                                 <  1:122345/118‑1 (MQ=255)
                                                                                                gtagtGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAGCAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCtt                                                                 >  2:122559/1‑118 (MQ=255)
                                                                                                gtagtGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAGCAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCtt                                                                 <  1:122559/118‑1 (MQ=255)
                                                                                                     gtAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATTTTTCACTTCTAGCAACATg                                     >  2:513590/1‑141 (MQ=255)
                                                                                                         aCTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCAt                                 <  1:710424/141‑1 (MQ=255)
                                                                                                                   cccAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCAtt                       <  2:509761/141‑1 (MQ=255)
                                                                                                                      aGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCt                    <  2:224590/141‑1 (MQ=255)
                                                                                                                         cTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTg                 <  2:517542/141‑1 (MQ=255)
                                                                                                                               cATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTGTTAGtt           <  1:221863/141‑1 (MQ=255)
                                                                                                                                aTAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTGTTAGTTc          <  1:555818/141‑1 (MQ=255)
                                                                                                                                aTAATTGCAACAAACTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTCTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTGTTAGTTc          <  2:5349/141‑1 (MQ=255)
                                                                                                                                 tAATTGCATCAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTGTTAGTTCt         <  2:567533/141‑1 (MQ=255)
                                                                                                                                        aacaaATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTGTTAGTTCTATATTaa  >  1:228011/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ACAATGCTTTACTTTTATTTTTCATATTATTTAAATGTGCGCCATCCACAATAATGTCACCTTCAGTTAGTCCATCAAAAGAAGCAATTAAATTTAGTAGTGTAGACTTCCCTGACCCAGACTCACCCATAATTGCAACAAATTCACCAGCTCCAACTGATAAATTCATTTGATTAAGTGCTGTCGTAGCATTCAAACCTTTACCATAAACCTTTTTTACATGTTTCACTTCTAGCAACATGGCATGCACCTCATTTCTTTGTTAGTTCTATATTAA  >  CP000730/2768867‑2769143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑