Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,669,464 A→G 6.3% T122A (ACA→GCA)  yicL → DMT superfamily drug/metabolite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,669,4640AG 6.3% 188.3 / 3.5 64T122A (ACA→GCA) yicLDMT superfamily drug/metabolite transporter
Reads supporting (aligned to +/- strand):  ref base A (26/34);  new base G (2/2);  total (28/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

CAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTCAT  >  CP000730/2669326‑2669602
                                                                                                                                          |                                                                                                                                          
cAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACa                                                                                                                                          >  2:94013/1‑141 (MQ=255)
cAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACa                                                                                                                                          <  1:312785/141‑1 (MQ=255)
 aaCCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCAt                                                                                                                                         <  2:138075/141‑1 (MQ=255)
    ccAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGTTGCAATCGCAGCATtac                                                                                                                                      <  1:212354/141‑1 (MQ=255)
      aaaCGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATtacta                                                                                                                                    <  1:115931/141‑1 (MQ=255)
                       cGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGt                                                                                                                   <  2:483229/141‑1 (MQ=255)
                         tATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGttt                                                                                                                 >  1:366823/1‑141 (MQ=255)
                             ccAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTTGTTATCTCTGCTTATATGGCATCTATTAATACAGGTATTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTatat                                                                                                             <  1:493835/141‑1 (MQ=255)
                                aGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattat                                                                                                          >  2:225841/1‑141 (MQ=255)
                                 gATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattatt                                                                                                         <  1:386360/141‑1 (MQ=255)
                                   tCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTAtattattat                                                                                                       <  2:80434/141‑1 (MQ=255)
                                       cGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTgg                                                                                                   >  1:355484/1‑141 (MQ=255)
                                        gTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGt                                                                                                  >  2:663459/1‑141 (MQ=255)
                                           tGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTg                                                                                               >  1:463943/1‑141 (MQ=255)
                                                          ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                                                                                >  2:87416/1‑141 (MQ=255)
                                                          ttATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTg                                                                                >  2:482762/1‑141 (MQ=255)
                                                                 cAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACt                                                                         >  1:676680/1‑141 (MQ=255)
                                                                  aGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                        <  1:195176/141‑1 (MQ=255)
                                                                     ataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa                                                                     >  2:231827/1‑141 (MQ=255)
                                                                     ataCTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACa                                                                     >  2:290633/1‑141 (MQ=255)
                                                                         tGGGCATGTTGGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCCCTTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat                                                                 <  1:12386/141‑1 (MQ=255)
                                                                         tGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACattat                                                                 <  1:328547/141‑1 (MQ=255)
                                                                              aTGTTAGTAGTACAATATGCTTTTATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAt                                                            <  1:93210/141‑1 (MQ=255)
                                                                                 ttAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATGTTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGAtgtg                                                         >  2:90929/1‑141 (MQ=255)
                                                                                    gtagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgctt                                                      <  1:526090/141‑1 (MQ=255)
                                                                                     tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttg                                                     >  2:593081/1‑141 (MQ=255)
                                                                                     tagtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttg                                                     <  1:303465/141‑1 (MQ=255)
                                                                                      agtaCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGtgcttgc                                                    <  2:509852/141‑1 (MQ=255)
                                                                                        taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCta                                                  <  1:134585/141‑1 (MQ=255)
                                                                                        taCAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCta                                                  <  1:110451/141‑1 (MQ=255)
                                                                                           aaTATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCtatta                                               <  1:123015/141‑1 (MQ=255)
                                                                                               tGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCAt                                           <  1:586802/141‑1 (MQ=255)
                                                                                                gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                          <  2:223291/141‑1 (MQ=255)
                                                                                                gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                          <  1:467833/141‑1 (MQ=255)
                                                                                                gCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATg                                          >  2:83846/1‑141 (MQ=255)
                                                                                                 cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                        <  1:290817/110‑1 (MQ=255)
                                                                                                 cTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTa                                                                        >  2:290817/1‑110 (MQ=255)
                                                                                                         gCATCTAATGATAGAGGTAATGCTGCGATTGCAACTTTACTACAATACATTGCGCCAGTTTATATTATTATCTGGTTTGTCATAAGAGGCGTTGCAAAACTATCATTATTTGATGTGCTTGCTATTATCATGACACTATTa                                 >  2:365981/1‑141 (MQ=255)
                                                                                                           aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAgg                               <  1:469468/141‑1 (MQ=255)
                                                                                                           aTCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAgg                               >  1:62175/1‑141 (MQ=255)
                                                                                                               aTTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACa                           <  2:226667/141‑1 (MQ=255)
                                                                                                                 tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtt                         >  2:592302/1‑141 (MQ=255)
                                                                                                                 tAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtt                         <  1:663459/141‑1 (MQ=255)
                                                                                                                   attgtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                       >  1:523337/7‑141 (MQ=255)
                                                                                                                   attgtagTAATGTTGCAATCGCAGCATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                       >  1:523333/7‑141 (MQ=255)
                                                                                                                   aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                       <  1:518221/141‑1 (MQ=255)
                                                                                                                   aTACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACAtttt                       <  1:518247/141‑1 (MQ=255)
                                                                                                                       aGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTttatt                   >  1:515827/1‑141 (MQ=255)
                                                                                                                          tAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTttattatt                <  2:112653/141‑1 (MQ=255)
                                                                                                                            aTGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTaa              >  1:143816/1‑141 (MQ=255)
                                                                                                                             tgctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAAc             <  2:339061/141‑1 (MQ=255)
                                                                                                                              gctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACa            >  2:496152/1‑141 (MQ=255)
                                                                                                                              gctgcGATTGCAACATTACTACAATACATTGCGCCAGGTTATATTATTATTTGGTTCGTCATAAGAGGCGTTGCAAAACTAACATTATCTGATGTGCTTGCTATTATCATGACACTATTAGGCACATTTTTATTATTAACa            >  2:548428/1‑141 (MQ=255)
                                                                                                                               ctgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaa           >  1:104300/1‑141 (MQ=255)
                                                                                                                                tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaaa          >  2:607808/1‑141 (MQ=255)
                                                                                                                                tgcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACaaa          >  2:76279/1‑141 (MQ=255)
                                                                                                                                 gcGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAAt         >  2:58554/1‑141 (MQ=255)
                                                                                                                                  cGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATg        <  2:359295/141‑1 (MQ=255)
                                                                                                                                  cGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATg        <  2:85590/141‑1 (MQ=255)
                                                                                                                                      tGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTc    <  1:369618/141‑1 (MQ=255)
                                                                                                                                       gCAACATTAGTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTCa   <  2:282757/141‑1 (MQ=255)
                                                                                                                                       gCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTCa   <  1:402558/141‑1 (MQ=255)
                                                                                                                                        cAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTCAt  <  2:515827/141‑1 (MQ=255)
                                                                                                                                        cAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTCAt  <  1:634989/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
CAACCCAAACGTTCAATATTTAGCGTATTCCAAGATCGACGTATGTTAGGCAAATTACTTATCTTCAGTATACTGGGCATGTTAGTAGTACAATATGCTTATATGGCATCTATTAATACAGGTAATGCTGCGATTGCAACATTACTACAATACATTGCGCCAGTTTATATTATTATTTGGTTTGTCATAAGAGGCGTTGCAAAACTAACATTATTTGATGTGCTTGCTATTATCATGACACTATTAGGAACATTTTTATTATTAACAAATGGTTCAT  >  CP000730/2669326‑2669602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: