Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,636,965 A→T 7.5% I270I (ATT→ATA)  gntP ← GntP family gluconate:proton (H+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,636,9650AT 7.5% 189.8 / 4.8 62I270I (ATT→ATA) gntPGntP family gluconate:proton (H+) symporter
Reads supporting (aligned to +/- strand):  ref base A (27/30);  new base T (2/3);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CATCATTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTAAATTC  >  CP000730/2636827‑2637105
                                                                                                                                          |                                                                                                                                            
catcatTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAAt                                                                                                                                            <  1:7526/141‑1 (MQ=255)
  tcatTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGa                                                                                                                                          >  2:570299/1‑141 (MQ=255)
  tcatTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGa                                                                                                                                          >  2:570315/1‑141 (MQ=255)
   cactCCGATTGGATAAATCGCATTCGTCACTGATTCCATACTATGAGTCATCTTACGCTCTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGAt                                                                                                                                         <  1:285084/138‑1 (MQ=255)
    atTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATa                                                                                                                                        >  1:300059/1‑141 (MQ=255)
    atTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATa                                                                                                                                        >  1:597798/1‑141 (MQ=255)
       ccGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGAt                                                                                                                                     >  2:283476/1‑141 (MQ=255)
          aTTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCa                                                                                                                                  <  1:711002/141‑1 (MQ=255)
            tGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCaaa                                                                                                                                <  2:71324/141‑1 (MQ=255)
            tGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCaaa                                                                                                                                <  1:519348/141‑1 (MQ=255)
             ggATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAAt                                                                                                                               <  1:621522/141‑1 (MQ=255)
                  aaTCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGAtt                                                                                                                          >  2:491644/1‑141 (MQ=255)
                    tCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTg                                                                                                                        >  2:301652/1‑141 (MQ=255)
                                  gATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGc                                                                                                          <  1:570315/141‑1 (MQ=255)
                                  gATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGc                                                                                                          <  1:570299/141‑1 (MQ=255)
                                   aTTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATCTAAATGATAGATCCAAATAGATTTGTAGGTTTGTCATGcc                                                                                                         <  2:559493/141‑1 (MQ=255)
                                      ccATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAg                                                                                                      >  2:19806/1‑141 (MQ=255)
                                       cATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGt                                                                                                     <  2:62606/141‑1 (MQ=255)
                                             atGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCACAGTTACAAg                                                                                               >  1:464091/1‑141 (MQ=255)
                                             atGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCACAGTTACAAg                                                                                               >  1:378699/1‑141 (MQ=255)
                                              tGATTCATCTTACGTTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCGTGCCCAGTTACAAGt                                                                                              <  1:704437/141‑1 (MQ=255)
                                              tGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGt                                                                                              <  2:605482/141‑1 (MQ=255)
                                              tGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGt                                                                                              <  2:33314/141‑1 (MQ=255)
                                                 ttCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGc                                                                                           <  2:85391/141‑1 (MQ=255)
                                                 ttCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGc                                                                                           >  1:426175/1‑141 (MQ=255)
                                                             tttCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGac                                                                               <  2:669841/141‑1 (MQ=255)
                                                                 tcATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTa                                                                           >  2:616233/1‑141 (MQ=255)
                                                                  cATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTaa                                                                          >  1:168661/1‑141 (MQ=255)
                                                                    ttAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACa                                                                        >  1:43454/1‑141 (MQ=255)
                                                                    ttAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCAGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACa                                                                        <  2:687558/141‑1 (MQ=255)
                                                                      aaTCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGTTGTCCCTGGTGTTCCTATCATATATATGATAGATTCAAATTGATTTGTAGGTTTGTCATGCCGAGTTATAAGTTGCTTTATCGTAGACACTCACAtt                                                                      <  2:195461/141‑1 (MQ=255)
                                                                         cccATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTTCGTTATCGTAGACACTAACATTAat                                                                   >  2:293216/1‑141 (MQ=255)
                                                                             tCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGa                                                               <  2:225038/141‑1 (MQ=255)
                                                                               gTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACt                                                             <  1:64707/141‑1 (MQ=255)
                                                                                  aCGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGt                                                          <  2:678638/141‑1 (MQ=255)
                                                                                         cAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGCTTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATCAATATGACTGGTAATGTTg                                                   >  2:496358/1‑141 (MQ=255)
                                                                                           aataatACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCt                                                 >  1:562918/1‑141 (MQ=255)
                                                                                            ataataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTg                                                <  2:433949/141‑1 (MQ=255)
                                                                                             taataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGt                                               >  1:633882/1‑141 (MQ=255)
                                                                                             taataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGt                                               >  1:463661/1‑141 (MQ=255)
                                                                                             taataCTGCCATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGt                                               <  1:526208/141‑1 (MQ=255)
                                                                                               ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                      >  2:563325/1‑51 (MQ=37)
                                                                                               ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                      <  1:563281/52‑2 (MQ=37)
                                                                                               ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                      >  2:563281/1‑51 (MQ=37)
                                                                                               ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATTTATATGATAgg                                                                                                                                      <  1:563325/52‑2 (MQ=37)
                                                                                               ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGGTTTGTAGGTTTTTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTta                                             >  2:133581/1‑141 (MQ=255)
                                                                                               ataCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTta                                             >  1:149158/1‑141 (MQ=255)
                                                                                                taCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATACAAATGATAGATTCACCATGATTTGTCTCTTGTTCATGCCCATTTACAATTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTtaa                                            <  1:420124/141‑1 (MQ=255)
                                                                                                 aCTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTtaat                                           >  2:403948/1‑141 (MQ=255)
                                                                                                     cTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAAc                                       >  1:191361/1‑141 (MQ=255)
                                                                                                     cTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAAc                                       >  1:234594/1‑141 (MQ=255)
                                                                                                     cTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAAc                                       >  1:191150/1‑141 (MQ=255)
                                                                                                                gCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAAt                            >  1:526376/1‑141 (MQ=255)
                                                                                                                   gTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCt                         <  1:10624/141‑1 (MQ=255)
                                                                                                                   gTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCt                         <  1:10690/141‑1 (MQ=255)
                                                                                                                       ctgctgTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCa                     <  1:112083/141‑1 (MQ=255)
                                                                                                                         gctgTTCCTATCATATAAATGATAGATTCAAATTGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCAtc                   <  2:537067/141‑1 (MQ=255)
                                                                                                                         gctgTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCAtc                   <  2:279904/141‑1 (MQ=255)
                                                                                                                                  aTCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATcc          <  1:259610/141‑1 (MQ=255)
                                                                                                                                    cATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGt        <  1:447942/141‑1 (MQ=255)
                                                                                                                                    cATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGt        <  1:416996/141‑1 (MQ=255)
                                                                                                                                       ataAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTaaa     >  1:284131/1‑141 (MQ=255)
                                                                                                                                         aaaTGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTAAAtt   >  1:142130/1‑141 (MQ=255)
                                                                                                                                          aaTGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTAAATTc  >  2:70886/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                            
CATCATTCCGATTGGATAAATCGCATTCGTCACTGATTCCATAATATGATTCATCTTACGCTTTCTCATTAATCCCATCGTAACGATTGCAAATAATACTGCTATTAGCATGGCTGTCCCTGCTGTTCCTATCATATAAATGATAGATTCAAATAGATTTGTAGGTTTGTCATGCCCAGTTACAAGTTGCGTTATCGTAGACACTAACATTAATATGACTGGTAATGTTGCTGTTAATAAACTCATACCAAATCCTGGCATCTCTTGATCCGTAAATTC  >  CP000730/2636827‑2637105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑