Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,614,139	A→T	6.1%	S178T (TCA→ACA)	USA300HOU_2478 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,614,139	0	A	T	6.1%	210.0 / 4.2	66	S178T (TCA→ACA)	USA300HOU_2478	lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (34/28);  new base T (2/2);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

AATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCATTTTTACTG  >  CP000730/2614001‑2614279                                                                                                                                           |                                                                                                                                             aaTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGaaa                                                                                                                                            <  1:354861/141‑1 (MQ=255) aaTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGaaa                                                                                                                                            >  1:586216/1‑141 (MQ=255) aaTCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGaaa                                                                                                                                            >  1:457595/1‑141 (MQ=255)   tCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATg                                                                                                                                          >  1:129151/1‑141 (MQ=255)   tCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATg                                                                                                                                          <  2:245911/141‑1 (MQ=255)    cACCATCGCCTTTTATTAATAATTTAGGTACTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGTAATGt                                                                                                                                         >  2:137824/1‑141 (MQ=255)    cACCATCGCCTTTTATTAATAATTTAGGTACTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGTAATGt                                                                                                                                         >  2:137603/1‑141 (MQ=255)     aCCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTc                                                                                                                                        >  2:415466/1‑141 (MQ=255)     aCCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTc                                                                                                                                        <  2:126661/141‑1 (MQ=255)     aCCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAAATTGGCACATTCGGATTATATGAAATGTc                                                                                                                                        >  1:568288/1‑141 (MQ=255)      ccATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCa                                                                                                                                       <  1:474045/141‑1 (MQ=255)       taTCGCCTTTTATGAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACATCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCAc                                                                                                                                      >  2:283835/2‑141 (MQ=255)         tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                                                                    >  1:454779/1‑141 (MQ=255)         tCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCa                                                                                                                                    >  2:299974/1‑141 (MQ=255)           gCCTTTTATTAATAATTTAGGTGCTTACTTAGTTCGTATATCATACCTCTTTATAAGTTGCTTCACATTGAAGTCAACATTAATTAATTGATACTTTGCTGAATAACTTGGCACAATCGGATTATATGAAATGTCACCATc                                                                                                                                  <  1:211128/141‑1 (MQ=255)                ttattaATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATATTaat                                                                                                                             >  1:98394/1‑141 (MQ=255)                  attaATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAACTGTCAACATCTTTGTaa                                                                                                                           >  2:25834/1‑141 (MQ=255)                      ataatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCAt                                                                                                                       >  1:242810/1‑141 (MQ=255)                        aatTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTa                                                                                                                     >  1:97068/1‑141 (MQ=255)                          tttAGGTGCTTTTTTAGTTGGTATATCATATCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGACTCCCTTGGGACTTTAGGCTTATATGAAATGTCACCATCTTTATTATCATTAat                                                                                                                   <  2:274331/141‑1 (MQ=255)                              ggTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTAATCATTACTTAATTGATACTTTGCTGAATAACTTGTCACATTCGTATTATATGAAATGTCACCATCTTTATAATCATTAATATCt                                                                                                               >  1:136239/1‑141 (MQ=255)                                tGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCttt                                                                                                             <  2:521787/141‑1 (MQ=255)                                tGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCttt                                                                                                             <  2:558126/141‑1 (MQ=255)                                               tataTCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTg                                                                                              <  1:466930/141‑1 (MQ=255)                                                     aTACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACaaa                                                                                        <  2:452631/141‑1 (MQ=255)                                                        ccTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGaa                                                                                     >  2:70566/1‑141 (MQ=255)                                                        ccTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGaa                                                                                     >  1:566063/1‑141 (MQ=255)                                                        ccTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGaa                                                                                     >  1:143518/1‑141 (MQ=255)                                                          tctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAtt                                                                                   >  1:549669/1‑141 (MQ=255)                                                          tctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAtt                                                                                   <  2:161604/141‑1 (MQ=255)                                                          tctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAtt                                                                                   <  1:644556/141‑1 (MQ=255)                                                           ctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAttt                                                                                  <  2:375302/141‑1 (MQ=255)                                                           ctTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAAttt                                                                                  <  2:618063/141‑1 (MQ=255)                                                              tCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaaa                                                                               <  2:401462/141‑1 (MQ=255)                                                              tCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTaaa                                                                               >  2:298551/1‑141 (MQ=255)                                                                  aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                                                                           >  1:125948/1‑141 (MQ=255)                                                                  aGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGttt                                                                           <  1:426460/141‑1 (MQ=255)                                                                   gTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGtttt                                                                          >  1:230028/1‑141 (MQ=255)                                                                   gTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGtttt                                                                          >  1:622647/1‑141 (MQ=255)                                                                   gTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGtttt                                                                          >  2:567757/1‑141 (MQ=255)                                                                      gCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGa                                                                       <  2:682217/141‑1 (MQ=255)                                                                          cacaTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttc                                                                   <  2:382131/141‑1 (MQ=255)                                                                             aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                                                                <  1:177193/141‑1 (MQ=255)                                                                             aTTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttt                                                                <  1:396827/141‑1 (MQ=255)                                                                              ttGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttctttt                                                               <  1:436732/141‑1 (MQ=255)                                                                              ttGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttctttt                                                               >  1:290576/1‑141 (MQ=255)                                                                               tGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGAtttcttttc                                                              >  2:19027/1‑141 (MQ=255)                                                                                  aGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTa                                                           <  1:446473/141‑1 (MQ=255)                                                                                   gTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTaa                                                          >  2:601836/1‑141 (MQ=255)                                                                                     catcatTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAAtt                                                        <  1:515534/141‑1 (MQ=255)                                                                                                  ttGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGTAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTAt                                           <  1:137824/141‑1 (MQ=255)                                                                                                  ttGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGTAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTAt                                           <  1:137603/141‑1 (MQ=255)                                                                                                    gATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCg                                         <  1:216290/141‑1 (MQ=255)                                                                                                     aTACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCgg                                        <  1:70566/141‑1 (MQ=255)                                                                                                             cTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTttggttg                                >  2:58413/1‑141 (MQ=255)                                                                                                                  tAACTTGGCACATTCGGATTAGATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATa                           >  2:499339/1‑141 (MQ=255)                                                                                                                   aaCTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATaa                          >  1:26564/1‑141 (MQ=255)                                                                                                                    aCTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAAt                         >  2:141425/1‑141 (MQ=255)                                                                                                                     cTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAAtt                        <  1:550984/141‑1 (MQ=255)                                                                                                                       tGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAAtttt                      >  2:221018/1‑141 (MQ=255)                                                                                                                         gCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTttat                    >  2:592618/1‑141 (MQ=255)                                                                                                                           acaTTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTttatta                  >  1:48272/1‑141 (MQ=255)                                                                                                                                  gATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCa           <  1:249959/141‑1 (MQ=255)                                                                                                                                   aTTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCAt          >  1:680664/1‑141 (MQ=255)                                                                                                                                      atatGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAAtt                        >  1:471327/1‑124 (MQ=255)                                                                                                                                      atatGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAAtt                        <  2:471327/124‑1 (MQ=255)                                                                                                                                      atatGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCAtttt       >  2:399078/1‑141 (MQ=255)                                                                                                                                       tatGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCAttttt      >  2:169321/1‑141 (MQ=255)                                                                                                                                         tGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCATTTTTAc    <  1:505649/141‑1 (MQ=255)                                                                                                                                           aaaTGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCATTTTTACTg  <  1:495593/141‑1 (MQ=255)                                                                                                                                           |                                                                                                                                             AATCACCATCGCCTTTTATTAATAATTTAGGTGCTTTCTTAGTTGGTATATCATACCTCTTTCTAAGTTGCTTCACATTGTAGTCATCATTACTTAATTGATACTTTGCTGAATAACTTGGCACATTCGGATTATATGAAATGTCACCATCTTTATAATCATTAATATCTTTAAAGTCACCGTATTGTACAAAGAATTTAAAGTTTTCGATTTCTTTTCTTAATTTATCATCAGCTATCGGTTTGGTTGGGATAATTTTATTATGTTCCATTTTTACTG  >  CP000730/2614001‑2614279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑