Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,755,685 Δ1 bp 8.0% intergenic (‑82/+273) obg ← / ← rpmA GTP‑binding protein/ribosomal protein L27

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,755,6850A. 8.0% 152.7 / 8.0 50intergenic (‑82/+273)obg/rpmAGTP‑binding protein/ribosomal protein L27
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base . (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.57e-01

AATACCATTACCACCATCACCGGCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTAGGGGCTCT  >  CP000730/1755547‑1755825
                                                                                                                                          |                                                                                                                                            
aaTACCATTACCACCATCACCGGCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAgg                                                                                                                                            <  1:586408/141‑1 (MQ=255)
aaTACCATTACCACCATCACCGGCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAgg                                                                                                                                            <  1:432743/141‑1 (MQ=255)
 aTACCATTACCACCATCACCGGCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAggg                                                                                                                                           >  1:371498/1‑141 (MQ=255)
      aTTACCACCATCACCGGCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGctct                                                                                                                                      >  2:201687/1‑141 (MQ=255)
              caTCACCGGCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGt                                                                                                                              >  2:605853/1‑141 (MQ=255)
                      gCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTta                                                                                                                      <  2:233018/141‑1 (MQ=255)
                       cTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTtat                                                                                                                     >  1:623751/1‑141 (MQ=255)
                         ttAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATaa                                                                                                                   >  1:525686/1‑141 (MQ=255)
                             gagaTATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCt                                                                                                               >  1:86149/1‑141 (MQ=255)
                             gagaTATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCt                                                                                                               >  2:69955/1‑141 (MQ=255)
                                 tatTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGc                                                                                                           <  1:96170/141‑1 (MQ=255)
                                    tttGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATT‑GGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTa                                                                                                       >  2:35387/1‑141 (MQ=255)
                                    tttGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATT‑GGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTa                                                                                                       >  2:35380/1‑141 (MQ=255)
                                            gATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACt                                                                                                <  1:526625/141‑1 (MQ=255)
                                                  cAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATAtt                                                                                          >  1:454397/1‑141 (MQ=255)
                                                   aaaCATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATAttt                                                                                         >  1:452580/1‑141 (MQ=255)
                                                         atatCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGc                                                                                   >  1:384388/1‑141 (MQ=255)
                                                                 ccTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATtg                                                                           <  1:426113/141‑1 (MQ=255)
                                                                    ctTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACtt                                                                                               <  2:153953/118‑1 (MQ=255)
                                                                    ctTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACtt                                                                                               >  1:153953/1‑118 (MQ=255)
                                                                    ctTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTgg                                                                        <  1:417393/141‑1 (MQ=255)
                                                                     ttttACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGt                                                                       >  2:328723/1‑141 (MQ=255)
                                                                       ttACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTAc                                                                     <  1:603032/141‑1 (MQ=255)
                                                                           atatAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGtt                                                                 >  1:304285/1‑141 (MQ=255)
                                                                                  tttcattttcCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTTTCAGCACCAATTGTGGTACAGTTTATTCtt                                                          >  1:641912/1‑141 (MQ=255)
                                                                                     cattttcCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTaa                                                       >  1:191774/1‑141 (MQ=255)
                                                                                     cactctcCCACTAATTATCGCATATTAGCGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGTAGTTACTTTTATAACACTTTGCTTTAAAATACTTCTATTTCTCTGCACCAATTGTGGTACAGCTTATTCTTTaa                                                       <  2:709533/136‑1 (MQ=255)
                                                                                           cccAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTg                                                 >  1:648328/1‑141 (MQ=255)
                                                                                           cccAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATT‑GGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGc                                                <  1:35380/141‑1 (MQ=255)
                                                                                           cccAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATT‑GGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGc                                                <  1:35387/141‑1 (MQ=255)
                                                                                                 aatTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTg                                           <  1:118439/141‑1 (MQ=255)
                                                                                                 aatTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTg                                           <  1:687088/141‑1 (MQ=255)
                                                                                                 aatTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTg                                           <  2:659010/141‑1 (MQ=255)
                                                                                                 aatTAGAGCATATTAGAGCCCCTTATATCTTGTAAATAATTAGGGGCTCTTATGAAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTg                                           <  2:127971/141‑1 (MQ=255)
                                                                                                    tataGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTAt                                        >  1:541882/1‑141 (MQ=255)
                                                                                                     ataGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTAtt                                       >  1:228105/1‑141 (MQ=255)
                                                                                                      taGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTAt                                        <  1:572948/139‑1 (MQ=255)
                                                                                                      taGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTAt                                        >  2:572948/1‑139 (MQ=255)
                                                                                                      taGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTc                                      >  2:648443/1‑141 (MQ=255)
                                                                                                               agagCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTccc                             >  1:577495/1‑141 (MQ=255)
                                                                                                                  gCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCt                          >  2:544499/1‑141 (MQ=255)
                                                                                                                  gCCCCTTATATATTGTAAATAATTAGGGGCTCTGATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCt                          <  2:537881/141‑1 (MQ=255)
                                                                                                                   ccccTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCtt                         <  2:208573/141‑1 (MQ=255)
                                                                                                                     ccTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTtat                       >  1:25955/1‑141 (MQ=255)
                                                                                                                             atTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGt               >  1:536115/1‑141 (MQ=255)
                                                                                                                                  aaaTAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTa          <  2:304285/141‑1 (MQ=255)
                                                                                                                                    ataatTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTCATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTAgg        <  1:493081/141‑1 (MQ=255)
                                                                                                                                     taatTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTAggg       <  2:623751/141‑1 (MQ=255)
                                                                                                                                       atTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTAGGGGc     >  1:702835/1‑141 (MQ=255)
                                                                                                                                        ttAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTAGGGGct    <  1:607026/141‑1 (MQ=255)
                                                                                                                                          aGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTAGGGGctct  <  2:89128/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
AATACCATTACCACCATCACCGGCTTTAAGAGATATTTTGACTTGATCGACAAACATATATCTCACCTCTTTTACATATAATTTTCATTTTCCCAATAATTATAGCATATTAGAGCCCCTTATATATTGTAAATAATTAGGGGCTCTTATGCAGTTACTTTTATAAATCTTTGCTTTAAAATACTTATATTTCTCAGCACCAATTGTGGTACAGTTTATTCTTTAAACGCTGCAACTGTATTCCTTTGTCCCCCTTATAGTTTAGTGATTAGGGGCTCT  >  CP000730/1755547‑1755825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑