Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,586,762 T→C 6.7% E94G (GAA→GGA)  USA300HOU_RS07780 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,586,7620TC 6.7% 197.6 / 3.3 72E94G (GAA→GGA) USA300HOU_RS07780hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/34);  new base C (2/3);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

ACGCTACCGCTTTAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATGGTCCATATACAATA  >  USA300TCH1516_ALE/1586625‑1586902
                                                                                                                                         |                                                                                                                                            
aCGCTACCGCTTTAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAg                                                                                                                                           >  2:709388/1‑141 (MQ=255)
 cGCTACCGCTTTAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAgg                                                                                                                                          <  2:550441/141‑1 (MQ=255)
  gCTACCGCTTTAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGc                                                                                                                                         <  2:120507/141‑1 (MQ=255)
      ccGCTTTAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATCCTTGAGCCTTATTACTTTCTTCTGATCTTCAGGCCACt                                                                                                                                     >  1:486536/1‑141 (MQ=255)
        gCTTTAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACttt                                                                                                                                   <  1:524235/141‑1 (MQ=255)
          tttAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTaa                                                                                                                                 >  1:287047/1‑141 (MQ=255)
           ttAACAAAGTTCTTTCGTATAGTAGTAACAGTATGACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAg                                                                                                                                <  2:202108/141‑1 (MQ=255)
             aataaaGTTCTTTCGTATAGTAGTAACAGTATTACTATGCATATGTCATTCATGTCCTATCTGTTACATGGTTAACTTCTTTTCTTTATTCCAATACTTGATCCTTATTACTTTCCTATGATCGTCAGGCAACTTTAAGTa                                                                                                                              <  2:677788/138‑1 (MQ=255)
             aaCAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTa                                                                                                                              >  1:298051/1‑141 (MQ=255)
                aaaGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTTCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTc                                                                                                                           <  1:367845/141‑1 (MQ=255)
                  aGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAACCTTATTACCTTCTTCTTATCTTCCGGCACCTTTAAGTATTAAc                                                                                                                         >  2:425905/1‑141 (MQ=255)
                      cTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACtctc                                                                                                                     >  1:578159/1‑141 (MQ=255)
                        ttCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCACCTTTAATTATTCACTCTCaa                                                                                                                   >  2:575320/1‑141 (MQ=255)
                        ttCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTACGTATTCACTCTCaa                                                                                                                   >  1:347890/1‑141 (MQ=255)
                        ttCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCaa                                                                                                                   >  1:556485/1‑141 (MQ=255)
                        ttCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTGCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCaa                                                                                                                   >  1:418590/1‑141 (MQ=255)
                               agtagtAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTc                                                                                                            >  1:208972/1‑141 (MQ=255)
                                gtagtaACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCg                                                                                                           >  1:219371/1‑141 (MQ=255)
                                     aaCAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGTTCTCCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCAt                                                                                                      >  2:39647/1‑141 (MQ=255)
                                     aaCAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTCCAGTCAACTTTAATTATTCACTCTCAACTGATTCGcccca                                                                                                      >  2:43260/1‑139 (MQ=255)
                                      aCAGTATTACGATGCATTTTGCATTCATGTACTATCTGTTCCATCTTTAACTTCTTTTTTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACGTTAAGGATTCACTCTCTACTGCTTCGACCAtt                                                                                                     <  2:151145/141‑1 (MQ=255)
                                         gTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTATTCAGTTCTTCAGGCACCTTTTAGTATTCACTCTCAACTGCTTCGACCATttct                                                                                                  >  1:569303/1‑141 (MQ=255)
                                              aCGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTCTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAg                                                                                             <  1:541336/141‑1 (MQ=255)
                                                gATGCATATGGCATTCATGTCCTATCTGTTCTATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATTATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGt                                                                                           >  2:682577/1‑141 (MQ=255)
                                                gATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGt                                                                                           >  1:131369/1‑141 (MQ=255)
                                                 aTGCATATCGCATTCATGTCCTATCTGGTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGtt                                                                                          <  1:427230/141‑1 (MQ=255)
                                                    cATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCg                                                                                       <  1:709388/141‑1 (MQ=255)
                                                      tatGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGGGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTa                                                                                     <  2:745570/141‑1 (MQ=255)
                                                      tatGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTa                                                                                     <  2:73773/141‑1 (MQ=255)
                                                      tatGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTa                                                                                     <  1:400389/141‑1 (MQ=255)
                                                        tGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAATTCTTCACTCTCAACTGCTTCCACCATTTCTTCAAGTTTTCGTCAc                                                                                   >  2:118350/1‑141 (MQ=255)
                                                         ggCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACa                                                                                  >  1:6585/1‑141 (MQ=255)
                                                             ttCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCtt                                                                              <  1:134200/141‑1 (MQ=255)
                                                             ttCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCGTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCtt                                                                              <  2:18777/141‑1 (MQ=255)
                                                                  gTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAg                                                                         <  1:351392/141‑1 (MQ=255)
                                                                       aacTGTTGCATGGTTAACTTCTGTAGTGGAGTCCAATACTTAAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACAATTTCTTCAAGGTTTCGTAAAATCTTATTAGTtaaa                                                                    >  2:764914/3‑140 (MQ=255)
                                                                       aTCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTtaat                                                                    <  2:208972/141‑1 (MQ=255)
                                                                          tGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTaataat                                                                 <  1:556935/141‑1 (MQ=255)
                                                                            ttCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTAGACCATTTTTTCAAGTTTTCGTAGCATCTTATTTGTTATTTATCt                                                               >  1:146932/1‑141 (MQ=255)
                                                                               cATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTACAATATTCTTAGTTAATAATCTAGt                                                            >  2:103680/1‑141 (MQ=255)
                                                                               cATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGt                                                            >  1:428662/1‑141 (MQ=255)
                                                                                   tttAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTCCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGcc                                                        <  1:39647/141‑1 (MQ=255)
                                                                                   tttAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTCCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGcc                                                        <  1:43260/141‑1 (MQ=255)
                                                                                      aaCTTCTTTTCTTTATTCGAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCAtt                                                     <  2:32146/141‑1 (MQ=255)
                                                                                      aaCTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCAtt                                                     <  2:649358/141‑1 (MQ=255)
                                                                                      aaCTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTCATTTTTTGGTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCAtt                                                     >  1:533605/1‑141 (MQ=255)
                                                                                         ttcttttcttTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAATGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGt                                                  <  1:115265/141‑1 (MQ=255)
                                                                                         ttcttttcttTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGt                                                  <  2:68135/141‑1 (MQ=255)
                                                                                         ttcttttcttTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGt                                                  <  1:591839/141‑1 (MQ=255)
                                                                                          tcttttctttATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATCATCTAGTTGCCATTAGtt                                                 >  2:198964/1‑141 (MQ=255)
                                                                                           cttttctttATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTc                                                <  2:90611/141‑1 (MQ=255)
                                                                                              ttctttATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATACTCTAGTTGCCATTAGTTCAgt                                             >  1:697427/1‑141 (MQ=255)
                                                                                               tctttATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAgtt                                            <  1:661891/141‑1 (MQ=255)
                                                                                                    aTTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCt                                       <  1:484434/141‑1 (MQ=255)
                                                                                                    aTTCCAAGACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCt                                       <  1:548069/141‑1 (MQ=255)
                                                                                                       tctaTACTTGTGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCATCTGCTTCGACCATTTCTTCCCGGTCTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAAc                                    <  2:518421/138‑1 (MQ=255)
                                                                                                       ccAAAACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAAc                                    <  2:270983/141‑1 (MQ=255)
                                                                                                          aTACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTgg                                 >  1:741652/1‑141 (MQ=255)
                                                                                                          aTACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTgg                                 >  1:599589/1‑141 (MQ=255)
                                                                                                                   ccTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCttt                        >  2:545613/1‑141 (MQ=255)
                                                                                                                   ccTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAAATACATCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTCGTTCAGTTGTTCTAACTGGTTCTTCttt                        >  2:132876/1‑141 (MQ=255)
                                                                                                                      tattaCTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTg                     >  1:554680/1‑141 (MQ=255)
                                                                                                                      tattaCTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTg                     >  1:544656/1‑141 (MQ=255)
                                                                                                                       attaCTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTCGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTg                     >  2:73053/1‑140 (MQ=255)
                                                                                                                       attaCTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTg                     <  1:73053/140‑1 (MQ=255)
                                                                                                                       attaCTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGt                    <  1:507970/141‑1 (MQ=255)
                                                                                                                            tttCTTTTGATCTCTAAGTAACTTTAATTCGTTATTCTCAACTGCTTCTACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATTATCTTGTTGTCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATgg               <  2:262821/141‑1 (MQ=255)
                                                                                                                            tttCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTTGCCTTTTTGTAATgg               >  1:623196/1‑141 (MQ=255)
                                                                                                                              tCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATACTATAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATGGTc             >  1:447142/1‑141 (MQ=255)
                                                                                                                                 tATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATGGTCCat          <  1:115783/141‑1 (MQ=255)
                                                                                                                                 tATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATGGTCCat          <  1:121042/141‑1 (MQ=255)
                                                                                                                                  aTGGTCTTCAGGCAACGTTATGTATTCACTCTCAACTTCTTCGACCATTTCTTCAAGGTTTTGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATGGTCCata         <  2:208333/141‑1 (MQ=255)
                                                                                                                                         tCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATGGTCCATATACAata  >  1:644609/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
ACGCTACCGCTTTAACAAAGTTCTTTCGTATAGTAGTAACAGTATTACGATGCATATGGCATTCATGTCCTATCTGTTCCATCTTTAACTTCTTTTCTTTATTCCAATACTTGAGCCTTATTACTTTCTTATGATCTTCAGGCAACTTTAAGTATTCACTCTCAACTGCTTCGACCATTTCTTCAAGGTTTCGTAACATCTTATTAGTTAATAATCTAGTTGCCATTAGTTCAGTTGTTCTAACTGGTTCGCCTTTTTGTAATGGTCCATATACAATA  >  USA300TCH1516_ALE/1586625‑1586902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑