Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 465,678 A→G 6.0% intergenic (+62/‑318) lpl2_3 → / → lpl2_4 putative lipoprotein/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE465,6780AG 6.0% 195.4 / 2.4 67intergenic (+62/‑318)lpl2_3/lpl2_4putative lipoprotein/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (30/32);  new base G (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

AAAGAAATATCCAGTGAAATTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCAAAATTATTGTTTAAGGGA  >  USA300TCH1516_ALE/465538‑465818
                                                                                                                                            |                                                                                                                                            
aaaGAAATATCAACAGAAATGAGAGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATgaga                                                                                                                                              <  2:638228/141‑1 (MQ=255)
    aaaTATCCAGTGAAATTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTAc                                                                                                                                          <  1:382399/141‑1 (MQ=255)
       tatCCAGTGAAATTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAACACAAAAAGTTAAAAAAATGAAATAGAGTCTTTTGAATTATTTTCACAATATGGAAACTATACACATTATAATCGGAATGACATTACTaa                                                                                                                                       >  2:179732/1‑141 (MQ=255)
          ccAGTGAAATTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAatat                                                                                                                                    >  1:741672/1‑141 (MQ=255)
           cAGTGAAATTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATAtt                                                                                                                                   <  2:356084/141‑1 (MQ=255)
            aGTGAAATTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATAttt                                                                                                                                  <  1:711161/141‑1 (MQ=255)
             gtaaaaTTGGTAAATAATATGAGAGTTTTGATAAAATATGTTAAAGAAAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTc                                                                                                                                 <  2:383050/138‑1 (MQ=255)
                 aaTTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCatat                                                                                                                             >  1:172299/1‑141 (MQ=255)
                   ttGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGGTTACTAATATTTCATATaa                                                                                                                           >  1:524815/1‑141 (MQ=255)
                   ttGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGGTTACTAATATTTAATCTaa                                                                                                                           >  2:4192/1‑141 (MQ=255)
                      gTGAATAATAGGATAGTTTTGATAAAATATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGATTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATcc                                                                                                                        <  1:288905/141‑1 (MQ=255)
                       tGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGGGGCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCt                                                                                                                       >  1:496574/1‑141 (MQ=255)
                       tGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCt                                                                                                                       <  1:374215/141‑1 (MQ=255)
                       tGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCt                                                                                                                       <  2:318742/141‑1 (MQ=255)
                       tGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCt                                                                                                                       <  1:221102/141‑1 (MQ=255)
                       ggAATAATAGAATAGTAAAAATAATATGTGTTGATTATAAAAAGTTAAAAAAATGTGATCGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCt                                                                                                                       <  2:549898/140‑1 (MQ=255)
                                ggATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTccc                                                                                                              <  1:669540/141‑1 (MQ=255)
                                    aGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAAtt                                                                                                          <  2:296686/141‑1 (MQ=255)
                                      ttttGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGCTTACTAATATTTCATATAATCCTAATGCTCCCAATTAc                                                                                                        <  1:540336/141‑1 (MQ=255)
                                      ttttGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTAc                                                                                                        >  2:261061/1‑141 (MQ=255)
                                      ttttGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTAc                                                                                                        <  1:247639/141‑1 (MQ=255)
                                        ttGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATCACTAATATTTCATATAATCCTAATTCTCCCAATTAatc                                                                                                      >  2:368924/1‑141 (MQ=255)
                                         tGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGGCAGATTAATCATTTTGATCGGAATGAGATTAATAATATTTCATATAATCCTCAGTCTCCCAATAActct                                                                                                     >  1:369774/1‑141 (MQ=255)
                                         tGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTActct                                                                                                     >  1:282882/1‑141 (MQ=255)
                                             aaaaGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTACCAATTACTCTGCAg                                                                                                 >  1:531458/1‑141 (MQ=255)
                                             aaaaGATGTAAAAGACAAACAGTTAAAAAAAAGAAATAGAGTCGTTTGAATTATTTTCATAATATGTAAACTTTAATCATTTTGATCGGCATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAg                                                                                                 <  1:79994/141‑1 (MQ=255)
                                                aGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAat                                                                                              >  2:37722/1‑141 (MQ=255)
                                                       aaaGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAAt                                                                                       <  2:586021/141‑1 (MQ=255)
                                                            cAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATgaaga                                                                                  <  1:52312/141‑1 (MQ=255)
                                                              aaaaGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGaaa                                                                                >  1:415616/1‑141 (MQ=255)
                                                                 aGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAAt                                                                             >  1:693377/1‑141 (MQ=255)
                                                                 aGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTACCAATTACTGAACAGCATAGAAAATGAAGAAAAAt                                                                             >  2:469670/1‑141 (MQ=255)
                                                                  gTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATg                                                                            <  2:172299/141‑1 (MQ=255)
                                                                    tAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGAc                                                                          <  1:37722/141‑1 (MQ=255)
                                                                    tAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGAc                                                                          <  1:54525/141‑1 (MQ=255)
                                                                    tAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGAc                                                                          <  1:348549/141‑1 (MQ=255)
                                                                             gAAATAGAGTCGTTTGAGTTCTTTCCAGACTATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATGACAGAAAATCCTAATGCGCCCAATTACTCTGCAGAAGAGAAAATGAAGAAAAATGACAGAAACAtt                                                                 >  2:203331/1‑141 (MQ=255)
                                                                                     gTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGGTTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGtt                                                         <  2:524815/141‑1 (MQ=255)
                                                                                     gTCGTGTCAATTATTTTCACAATATTGAAACTTTAATCATTTTGATCGGAATGAAGTTACTAATATTGCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGtt                                                         <  1:4192/141‑1 (MQ=255)
                                                                                     gTCGATTGAATTAGTTTGACAATAGGGAAACTTTAAGAATTTTGATCGGAATGAGATTACTAATATTTAATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGtt                                                         <  2:660991/141‑1 (MQ=255)
                                                                                      tCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTg                                                        >  1:183963/1‑141 (MQ=255)
                                                                                       cGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGa                                                       >  2:661966/1‑141 (MQ=255)
                                                                                         tttGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGaaa                                                     >  1:470157/1‑141 (MQ=255)
                                                                                           tGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATGTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCGACAGTTGaaaaa                                                   <  1:28620/141‑1 (MQ=255)
                                                                                           tGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAAATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGaaaaa                                                   <  2:184821/141‑1 (MQ=255)
                                                                                           tGAATTATGTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGaaaaa                                                   <  1:31994/141‑1 (MQ=255)
                                                                                            gAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAag                                                  >  1:52558/1‑141 (MQ=255)
                                                                                              attattTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAagag                                                >  1:322552/1‑141 (MQ=255)
                                                                                                    ttCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTaa                                          <  2:206752/141‑1 (MQ=255)
                                                                                                     tCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAAt                                         >  2:45319/1‑141 (MQ=255)
                                                                                                       acaATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCt                                       <  1:596779/141‑1 (MQ=255)
                                                                                                        aaaTATGTAATCTTGAATAATTTTTATAGTACTGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTa                                      <  2:271907/140‑1 (MQ=255)
                                                                                                                acaCTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAACAAAACTGACAGAAACATTCAACATTTGAAAAAGAAATTTACTATAAAAACTaa                              >  2:769347/3‑140 (MQ=255)
                                                                                                                aaaCTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGGTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAg                              >  2:535982/1‑141 (MQ=255)
                                                                                                                aaaCTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAg                              >  2:577885/1‑141 (MQ=255)
                                                                                                                   cTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCaa                           >  1:231972/1‑141 (MQ=255)
                                                                                                                       aaTGATTTTGATCGACATGAGATTAGTACTCTTTCATATAATCCTAATGCTACCAATTACTCTGCAGAATATAAACTGCTGAAAACTGAAAGATCCATTCACCAGTTGAAAAAGAGATTTAATCTAAACACTAGCacgcca                       <  2:375802/141‑6 (MQ=255)
                                                                                                                       aaTCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTACAGAATATAAAATGAAAAAAAATGACAGAAAAATTCAACAGTTAAAAAAGAAAATTAATCTAAAAATTAACAAGaaa                       >  2:226514/1‑139 (MQ=255)
                                                                                                                        aTCATTTTGATCGGAATGAGATTACTAATATTTCATATAATTTTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGacac                      >  1:614637/1‑141 (MQ=255)
                                                                                                                        aTCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATTAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGCGATTTAATCTAAAAACTAGCAAGacac                      >  2:61578/1‑141 (MQ=255)
                                                                                                                        aTCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGacac                      >  1:645965/1‑141 (MQ=255)
                                                                                                                        aTCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTAATCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGacac                      >  1:434902/1‑141 (MQ=255)
                                                                                                                            ttttGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCaaa                  <  1:59597/141‑1 (MQ=255)
                                                                                                                             tttGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCACTTAAGCTGCAGAATAACAACAGCCGCAGTATGTCAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCaaaa                 <  1:611634/141‑1 (MQ=255)
                                                                                                                             tttGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTACTCTAAAAACTAGCAAGACACCaaaa                 >  1:692432/1‑141 (MQ=255)
                                                                                                                             tttGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCaaaa                 <  1:206298/141‑1 (MQ=255)
                                                                                                                             tttGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCaaaa                 <  1:545459/141‑1 (MQ=255)
                                                                                                                                 aTCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCAAAattat             <  1:509079/141‑1 (MQ=255)
                                                                                                                                          agaTTACTAATATTTCATATCATTGCAGCAGTGCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGGGGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCAAAATTATTGTTTAAgg    >  2:478036/1‑141 (MQ=255)
                                                                                                                                            aTTACTAATATTTAATATAATCCTAATGATCCCAATTAATCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCAAAATTATTGTTTAAGGGa  <  2:353331/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAAGAAATATCCAGTGAAATTGGTGAATAATAGGATAGTTTTGATAAAAGATGTTAAAGACAAAAAGTTAAAAAAATGAAATAGAGTCGTTTGAATTATTTTCACAATATGGAAACTTTAATCATTTTGATCGGAATGAGATTACTAATATTTCATATAATCCTAATGCTCCCAATTACTCTGCAGAATATAAAATGAAGAAAAATGACAGAAACATTCAACAGTTGAAAAAGAGATTTAATCTAAAAACTAGCAAGACACCAAAATTATTGTTTAAGGGA  >  USA300TCH1516_ALE/465538‑465818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑