Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 197,615 G→A 6.1% V214I (GTT→ATT)  cmpC → Bicarbonate transport ATP‑binding protein CmpC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE197,6150GA 6.1% 220.1 / 3.4 73V214I (GTT→ATT) cmpCBicarbonate transport ATP‑binding protein CmpC
Reads supporting (aligned to +/- strand):  ref base G (34/34);  new base A (3/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

TAGCTATCATGAAATGTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCAAAGTGTAG  >  USA300TCH1516_ALE/197476‑197750
                                                                                                                                           |                                                                                                                                        
tAGCTATCATGAAATGTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGt                                                                                                                                         <  1:260911/141‑1 (MQ=255)
      tCATGAAATGTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATAcc                                                                                                                                   >  2:386441/1‑141 (MQ=255)
        aTGAAATGTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTg                                                                                                                                 <  1:54697/141‑1 (MQ=255)
             aTGTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCg                                                                                                                            <  1:474715/141‑1 (MQ=255)
             aTGTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCg                                                                                                                            <  1:75292/141‑1 (MQ=255)
               gTCGGCAGCAGAAATGCCAGGCGCATTGAGTGAAACCCGGAGTGCAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTa                                                                                                                          <  2:163836/141‑1 (MQ=255)
               gTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTGCAGGGTATTCTGTAGCTGAACCATTCGATACACTGGGTAAAAAGTTAGGCAAAGGTAAGCCTTTGAAACATTGTGATGACATTATACCTCAtcaata                                                                                                                          >  2:609269/1‑136 (MQ=255)
                 cgcgAGCAGGCATGCCAGCCGCATTGAGTGAACACAGACTTACAGGGTATTCTGTAGCCGACCCCTTCGGTGCACTGTGTGAAAAGTTAGGCAAAGGTAATTCTTTGCACCATGGTGTTGACGTTATACCTGATGCGTAtt                                                                                                                        <  2:521485/141‑1 (MQ=255)
                 cGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTAtt                                                                                                                        >  2:17888/1‑141 (MQ=255)
                 cGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTAtt                                                                                                                        >  1:433684/1‑141 (MQ=255)
                 cGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTAtt                                                                                                                        <  1:287346/141‑1 (MQ=255)
                 cGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTAtt                                                                                                                        <  1:286902/141‑1 (MQ=255)
                 cGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTAtt                                                                                                                        <  2:534985/141‑1 (MQ=255)
                  gCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTg                                                                                                                       <  1:712933/141‑1 (MQ=255)
                       cagAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCtgtg                                                                                                                  <  1:374959/141‑1 (MQ=255)
                           aaTGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTACGACTTTGAAACATCGTGATGACGTTATACCTGATGCGTCTCGCTGTGTGCt                                                                                                              >  2:89468/1‑141 (MQ=255)
                             tGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAg                                                                                                            <  1:744615/141‑1 (MQ=255)
                                 aGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACATTATACCTGATGCGTATTGCTGTGTGCTAGTACt                                                                                                        >  2:740097/1‑141 (MQ=255)
                                 aGCCGCATTGAGTGAACACAGAATTACAGGGTATACCGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACATTATACCTGATGCGTATTGCTGTGTGCTAGTACt                                                                                                        >  2:283211/1‑141 (MQ=255)
                                   gggCATTGAGTGAAGTCAGAATTGCAGGGTATTCTGTAGGCGAACGATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTga                                                                                                      <  1:752527/139‑1 (MQ=255)
                                   ccGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTga                                                                                                      >  1:376900/1‑141 (MQ=255)
                                       tgtGAGTGAACAGCGAAGGACAGGTTATTGTGTAGCGGAATGATTCGGTGGACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATAGCTGATGCGTATTGATGTGTGCTAGTATTGAGAgg                                                                                                  <  2:99345/139‑1 (MQ=255)
                                         ggtgTGAACGCAGAATTACAATGTATTCGGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGTAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAggg‑g                                                                                               <  2:450353/138‑1 (MQ=255)
                                                   caGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGa                                                                                     >  1:323775/1‑141 (MQ=255)
                                                   caGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGa                                                                                     <  1:716848/141‑1 (MQ=255)
                                                          aCAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTATGAAACATGGTGATGACGTTATACATGAAGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GCATTGCTTGATCaaaaa                                                                              >  2:657302/1‑138 (MQ=255)
                                                            aGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGCTCaagaaga                                                                            <  1:373660/141‑6 (MQ=255)
                                                                   tCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTa                                                                     <  1:763884/141‑1 (MQ=255)
                                                                    ttGTAGCCGAATCATTCTGTGTACTGTATGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATCCCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAg                                                                    <  2:192762/140‑1 (MQ=255)
                                                                     tGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAgc                                                                   >  2:498218/1‑141 (MQ=255)
                                                                       tAGCCGAACGATTCGGTGCGATGGGTGAAAAGTTCAGCAAAGGTAAGAGCTTGAAATAGTGTGATGACGTGATACGTGATGCGTATTGCTGTGTGGTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAgcgc                                                                 <  2:550531/141‑1 (MQ=255)
                                                                       tAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAgcgc                                                                 >  1:440494/1‑141 (MQ=255)
                                                                              aCCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTTCTGTGTGCTAGTCCTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCCAGCAtt                                                          >  1:450681/1‑141 (MQ=255)
                                                                              aCCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCAtt                                                          <  1:669167/141‑1 (MQ=255)
                                                                              aCCATTAGGTGAAATGAGTAAAAAGTTAGGCAAAGGTAAGAAACTAAAATATTTAAATGGCGGGAGACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCGTGATAAACACAAGGATGTAGCGCAAGCAtt                                                          >  2:761100/1‑141 (MQ=255)
                                                                                 aTTCGGTGCACGCGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGt                                                       <  1:602509/141‑1 (MQ=255)
                                                                                  ttCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTa                                                      >  1:422187/1‑141 (MQ=255)
                                                                                  ttCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGAAAGCATATGTa                                                      >  1:581106/1‑141 (MQ=255)
                                                                                  ttCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATATATTTCATGCATTTGTa                                                      >  2:580465/1‑141 (MQ=255)
                                                                                    cGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGGAGCGCAAGCATTTGTTCa                                                    >  2:572413/1‑141 (MQ=255)
                                                                                     ggTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GACTTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACaa                                                   >  1:138617/1‑141 (MQ=255)
                                                                                     ggTGCACTGGGAGAAAAGTTAAGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATACGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTTCTTGATCAAAACAAGGATGTAGCGCACGCATTTGTACaa                                                   >  2:96714/1‑141 (MQ=255)
                                                                                       tGCAGTGGATGAAAACCTGTGTAAAGTTAAGACGTTGAAACATGTGGATGACGTTATACCTGATGCGTTTTGCTGTGTGCTAGTACTTAGAGTG‑CATCTGCTTGATAAACACCCTGTTATAGCGCAAGCATTTGTACAAGa                                                 <  2:203790/141‑1 (MQ=255)
                                                                                          aCTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATta                                              <  2:704601/141‑1 (MQ=255)
                                                                                                   aaaGATAAGCAAAGGTAAGACATTGAAACAAGGTGATGACGAGATACTTGATGCGTACTGCAGAGTGCTAGAACGGAGAGGG‑GAATTGCTCGATCAACACAAGGATGTAGCGCAAGCATTTCTACAAAATTATaaaaaagc                                     >  2:495572/1‑138 (MQ=255)
                                                                                                     aGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTg                                   >  2:266918/1‑141 (MQ=255)
                                                                                                      gTTAAGAAAAGGTAAGACTTTGAAACTTGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTgg                                  <  2:339776/141‑1 (MQ=255)
                                                                                                       ttAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTTTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGACTATaaaaactatgac                                 >  2:155624/1‑134 (MQ=255)
                                                                                                        tAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGACGG‑GCATTTGTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCt                                <  1:699514/141‑1 (MQ=255)
                                                                                                         aGGCAAAGGTAAGACGTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCtt                               <  1:17888/141‑1 (MQ=255)
                                                                                                          ggCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCttt                              >  2:107887/1‑141 (MQ=255)
                                                                                                          ggCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCttt                              <  1:386441/141‑1 (MQ=255)
                                                                                                                   aaGACTTTGAAACATGGTGATGACGTTATACTTGATGCGGATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAaatgaat                     >  1:742760/1‑141 (MQ=255)
                                                                                                                   aaGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACCAGATTATAAAAAGTCTGGCTCTCAaatgaat                     >  2:170400/1‑141 (MQ=255)
                                                                                                                   aaGACTTTGAAACATGGTGATGACGTTATACCTGATGCGAATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAAGACAAGGATGTAGCGCAAGCATTGGTACAAGATTATAAAAAGTCGGGCTTTAAgatggct                     >  1:155133/1‑138 (MQ=255)
                                                                                                                      aCTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGAt                  >  1:605274/1‑141 (MQ=255)
                                                                                                                          tGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGGGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgca              <  1:170003/141‑1 (MQ=255)
                                                                                                                          tGAAACATGGTGATGACGTTATACCTGATGCGTATTACTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTATAGCTTTAAAATGAACGATCgca              >  2:461893/1‑141 (MQ=255)
                                                                                                                           gAAACGTGGTGATGACGTTATACCGGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAGAAGTCTGGCTTTAAAATGAATGATCgcaa             <  2:451933/141‑1 (MQ=255)
                                                                                                                            aaaCATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaag            >  1:152829/1‑141 (MQ=255)
                                                                                                                             aaCATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACGGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagc           >  2:696849/1‑141 (MQ=255)
                                                                                                                             aaCATGGTGATGACGTTATACCTCATGCGTATTGCTGTGTGCTAGTACTGAGTGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACCAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagc           >  2:668497/1‑141 (MQ=255)
                                                                                                                              aCATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagca          >  1:73041/1‑141 (MQ=255)
                                                                                                                               cATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGTG‑GAATTTCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagcaa         <  1:262408/141‑1 (MQ=255)
                                                                                                                               cATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGCTTATAAAAAGTCTGGCTTTAAAATGAATGATCgaaagcaa         >  2:350022/1‑141 (MQ=255)
                                                                                                                               cATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagcaa         >  1:689362/1‑141 (MQ=255)
                                                                                                                               cATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagcaa         <  1:326195/141‑1 (MQ=255)
                                                                                                                               cATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAAGG‑GATTTGCGTTATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagcaa         >  1:496749/1‑141 (MQ=255)
                                                                                                                               cATGGGCATGAGGTGATACCTGATGCGTAGTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCgcaagcaa         <  2:464941/141‑1 (MQ=255)
                                                                                                                                aTGGTGATGACATTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGGAGCAGT‑TTTGATCACCACAAGGATATAGCGGAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCaaa        <  1:740097/141‑1 (MQ=255)
                                                                                                                                aTGGTGATGACATTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCaaa        <  1:283211/141‑1 (MQ=255)
                                                                                                                                    tgatgaCGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCAAAatgt    >  2:732295/1‑141 (MQ=255)
                                                                                                                                      atgaCGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCAAAGTGTAg  <  1:498218/141‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TAGCTATCATGAAATGTCGCCAGCAGAAATGCCAGCCGCATTGAGTGAACACAGAATTACAGGGTATTCTGTAGCCGAACCATTCGGTGCACTGGGTGAAAAGTTAGGCAAAGGTAAGACTTTGAAACATGGTGATGACGTTATACCTGATGCGTATTGCTGTGTGCTAGTACTGAGAGGG‑GAATTGCTTGATCAACACAAGGATGTAGCGCAAGCATTTGTACAAGATTATAAAAAGTCTGGCTTTAAAATGAATGATCGCAAGCAAAGTGTAG  >  USA300TCH1516_ALE/197476‑197750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑