Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	632,919	A→G	G348G (GGA→GGG)	proP →	Proline/betaine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	632,919	0	A	G	100.0%	155.2 / NA	41	G348G (GGA→GGG)	proP	Proline/betaine transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/22);  total (19/22)

AGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCATT  >  USA300TCH1516_ALE/632787‑633050                                                                                                                                     |                                                                                                                                    aGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTCGTGGGCTAACATTACTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCtt                                                                                                                             <  1:342255/141‑1 (MQ=255)  gCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTa                                                                                                                            >  2:270534/1‑141 (MQ=255)              ggTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGaa                                                                                                                >  2:46157/1‑141 (MQ=255)                    aagaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACa                                                                                                          >  1:394261/1‑141 (MQ=255)                      gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  1:231451/1‑141 (MQ=255)                      gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  2:56614/1‑141 (MQ=255)                      gaaagTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTAGATTAGGGTTTTTCTTATCAACTTACGAAGCGACAAt                                                                                                        >  1:420229/1‑141 (MQ=255)                            aTTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAgg                                                                                                  <  1:152472/141‑1 (MQ=255)                                cTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAGTCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCg                                                                                              <  2:346647/141‑1 (MQ=255)                                cTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCg                                                                                              >  2:11039/1‑141 (MQ=255)                                  aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  1:282700/141‑1 (MQ=255)                                  aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:318139/141‑1 (MQ=255)                                  aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:387876/141‑1 (MQ=255)                                  aaTTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGtt                                                                                            <  2:387844/141‑1 (MQ=255)                                          cTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGa                                                                                    >  2:404865/1‑141 (MQ=255)                                             gtggGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGt                                                                                 <  1:382614/141‑1 (MQ=255)                                             gtggGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGt                                                                                 <  1:51423/141‑1 (MQ=255)                                               gggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGttt                                                                               <  2:278991/141‑1 (MQ=255)                                               gggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGttt                                                                               >  1:171617/1‑141 (MQ=255)                                                ggCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGtttt                                                                              <  2:392715/141‑1 (MQ=255)                                                 gCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTa                                                                             <  1:164511/141‑1 (MQ=255)                                                     aCATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGt                                                                         <  1:365793/141‑1 (MQ=255)                                                     aCATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGt                                                                         <  2:383922/141‑1 (MQ=255)                                                            tCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATaa                                                                  >  2:215743/1‑141 (MQ=255)                                                            tCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATaa                                                                  >  2:266273/1‑141 (MQ=255)                                                              aGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGa                                                                <  1:235696/141‑1 (MQ=255)                                                              aGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGa                                                                <  1:165810/141‑1 (MQ=255)                                                                  tcatcGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATc                                                            <  1:227405/141‑1 (MQ=255)                                                                               gTTATTACATTCACAATCGTGGTGTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  1:3852/141‑1 (MQ=255)                                                                               gTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  2:151026/141‑1 (MQ=255)                                                                               gTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGt                                               <  2:151702/141‑1 (MQ=255)                                                                                           acaATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACCTTTAatga                                   >  2:121816/1‑139 (MQ=255)                                                                                            caATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATAtc                                  >  1:271020/1‑141 (MQ=255)                                                                                            caATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATAtc                                  >  2:97291/1‑141 (MQ=255)                                                                                                          aaTAGTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATAtt                    >  2:110913/1‑141 (MQ=255)                                                                                                              gTAATCGGTATATTTATATTAGGGTTTTTCTTTTTTTCTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATTTTtggt                >  2:351948/1‑141 (MQ=255)                                                                                                              gTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTtggt                >  1:353413/1‑141 (MQ=255)                                                                                                              gTAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATAtggt                >  2:396968/1‑141 (MQ=255)                                                                                                               tAATCGGTATATTTATATTAGGGTTTTTCTTATCAACTTATGAAGCGACAATGCCAGGGGCGTTACCTACGATGTTTTACAGTCATATAAGGGATCGAACTTTATAAGTAACATTTAATATCTTTGTTGAGATATTtggtg               >  2:367447/1‑141 (MQ=255)                                                                                                                         atttatattAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGcc     <  2:171617/141‑1 (MQ=255)                                                                                                                            tatattAGGGTTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCAtt  <  1:404865/141‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    AGCGGATAAAATAGGTGAAAAGAAAGTATTTCTAATTGGTACTGGTGGGCTAACATTATTCAGTATCATCGCATTTATGTTATTACATTCACAATCATTTGTTGTAATAGTAATCGGTATATTTATATTAGGATTTTTCTTATCAACTTACGAAGCGACAATGCCAGGGTCGTTACCAACGATGTTTTACAGTCATATAAGATATCGAACTTTATCAGTAACATTTAATATCTCTGTTTCGATATTTGGTGGTACGACGCCATT  >  USA300TCH1516_ALE/632787‑633050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑