Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000731 25,669 (A)7→6 coding (322/333 nt) USA300HOU_pUSA300HOUMR0031 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073125,6630A.96.0% 441.0 / 6.5 101coding (316/333 nt)USA300HOU_pUSA300HOUMR0031hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/4);  new base . (51/46);  total (51/50)
Fisher's exact test for biased strand distribution p-value = 5.64e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

TGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAATAAAT  >  CP000731/25525‑25803
                                                                                                                                          |                                                                                                                                            
tgtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTCCTATACTGCAAATaaa                                                                                                                                            >  1:177587/1‑141 (MQ=255)
 gtatGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCATGTCTTGAAGGATCTTGGTTTTCTCAATATACAGAAATTTTCTTTATAATTGTCGCACTATTTCTTATGCTGCTTAATTACTCTACTACAAATaaaa                                                                                                                                           >  2:408122/1‑141 (MQ=255)
      ttATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAAATGTAGCACTATCTCCACTTATGCGTTATTACTATACTACAAATaaaaaatat                                                                                                                                      >  1:277798/1‑138 (MQ=255)
       tATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatata                                                                                                                                     >  2:167808/1‑137 (MQ=255)
        aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATAc                                                                                                                                   >  2:174129/1‑141 (MQ=255)
        aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCAAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaatatat                                                                                                                                    >  1:216468/1‑136 (MQ=255)
        aTTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATATTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATaaaaaaaataa                                                                                                                                    >  2:335482/1‑137 (MQ=255)
          ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACAt                                                                                                                                 <  1:60208/141‑1 (MQ=255)
          ttACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACAt                                                                                                                                 >  1:280221/1‑141 (MQ=255)
             cacTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTa                                                                                                                              >  1:151762/1‑141 (MQ=255)
              acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                             <  1:301688/141‑1 (MQ=255)
              acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                             <  1:288063/141‑1 (MQ=255)
              acTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTATAAAGATTTAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaa                                                                                                                             >  1:169225/1‑141 (MQ=255)
               cTAGTGATTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTaaa                                                                                                                            <  1:389642/141‑1 (MQ=255)
                  gtttgtAAATTGACAGGTTTGTCATCAAGTATTGAAGGATCTTGGTTTTATCAATAGTCAGAAATTTTCTTTCTAAGTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGt                                                                                                                         <  2:110932/136‑1 (MQ=255)
                     cTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATTCATCTAACGGttta                                                                                                                      >  1:41988/1‑139 (MQ=255)
                          aTTTACAGTTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTATTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTaa                                                                                                                 <  2:127459/141‑1 (MQ=255)
                           tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                                >  1:189340/1‑141 (MQ=255)
                           tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                                >  1:26172/1‑141 (MQ=255)
                           tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                                >  2:123301/1‑141 (MQ=255)
                           tttACAGGTTTGTCACCAAGTATTGAAGGATCTTGATTAGCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAAt                                                                                                                >  1:304521/1‑141 (MQ=255)
                            ttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCGAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAACATACATCGAAAGGTTAAATTAATg                                                                                                               <  1:305558/141‑1 (MQ=255)
                            ttACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGTTTAAATTAAta                                                                                                               >  2:179688/1‑140 (MQ=255)
                             tACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                              <  1:213179/141‑1 (MQ=255)
                             tACAAGTATGTCAACAAGTATTGAAGGATCTTAGTTTTCTCAATATCCAGAAATTTTATTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGa                                                                                                              <  2:295396/141‑1 (MQ=255)
                              aaaGGGTTGTAACCAAGTATTGAAGGATGTTGGTTTTCTCAATATACAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaa                                                                                                             <  2:209816/139‑1 (MQ=255)
                               cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAATATATACATCTAAAGGTTAAATTAATGaaa                                                                                                            >  2:288212/1‑141 (MQ=255)
                               cAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaa                                                                                                            >  1:93738/1‑141 (MQ=255)
                                aGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaaa                                                                                                           <  1:178851/141‑1 (MQ=255)
                                 ggTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGaaaaa                                                                                                          <  1:262407/141‑1 (MQ=255)
                                  gTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAAt                                                                                                         <  1:278623/141‑1 (MQ=255)
                                   tttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTCTTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATa                                                                                                        <  1:235710/141‑1 (MQ=255)
                                   tttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATa                                                                                                        <  1:399360/141‑1 (MQ=255)
                                   tttGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATa                                                                                                        <  1:396593/141‑1 (MQ=255)
                                    ttGTCATCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAg                                                                                                       <  2:18391/141‑1 (MQ=255)
                                      gTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGaa                                                                                                     >  2:45307/1‑141 (MQ=255)
                                       tCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAAt                                                                                                    >  1:283061/1‑141 (MQ=255)
                                         aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAAttn                                                                                                  >  1:313645/1‑140 (MQ=255)
                                         aCCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                                  >  2:34560/1‑141 (MQ=255)
                                         aCCAAGTATTGAAGGATCTGGAGTGTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTa                                                                                                  >  2:271146/1‑141 (MQ=255)
                                           cAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATATAAAGGTTAAGTTAATGAAACATAGAATTagt                                                                                                >  1:204848/1‑140 (MQ=255)
                                                aTTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAata                                                                                           <  1:368488/141‑1 (MQ=255)
                                                  tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                         >  1:368826/1‑141 (MQ=255)
                                                  tGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAtata                                                                                         >  1:967/1‑141 (MQ=255)
                                                    aatgATATTGGTTATGTCAATATACAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCAGTTTAATTACTATAATACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                       <  2:158100/138‑1 (MQ=255)
                                                    aaGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                       <  1:274014/141‑1 (MQ=255)
                                                    aaGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGa                                                                                       <  1:339380/141‑1 (MQ=255)
                                                        aattttgTTGTCTCAAAAAGCAGAAATTTTATGTATAATTGTAGCACAATTTTTTAAACGGTTAAATTACTATACTACAAATAAAAAAAATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAAc                                                                                   <  2:238500/135‑1 (MQ=255)
                                                          cTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaa                                                                                 >  1:389572/1‑141 (MQ=255)
                                                            tGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaa                                                                               >  2:204790/1‑141 (MQ=255)
                                                             ggTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACaaaaa                                                                              >  1:310361/1‑141 (MQ=255)
                                                                    tcAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAttt                                                                       >  1:402867/1‑141 (MQ=255)
                                                                    taaaTAACCAGAAATTTTCTTTAAAATTATAGCACTATTTCTTATACTGTTTAATTACTATACTAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAAttt                                                                       <  2:423094/139‑1 (MQ=255)
                                                                      aaTATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTCATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtct                                                                     >  1:346442/1‑141 (MQ=255)
                                                                       atatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTtctc                                                                    <  1:358456/141‑1 (MQ=255)
                                                                        tatCCAGAACTTTTCTTTATAATTGTATCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                                   <  1:158203/141‑1 (MQ=255)
                                                                        tatCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGCAATTtctaa                                                                   >  2:408915/1‑139 (MQ=255)
                                                                        taaccAGAAATTTTCTTTATAATTGTAGCAATATTTCTTATGCTGTTTAAATACTATACTAAAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCa                                                                   <  2:347431/138‑1 (MQ=255)
                                                                          tacaaaaaTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTGATATACTATAATACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                                 <  2:198180/136‑1 (MQ=255)
                                                                          tCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                                 <  1:185381/141‑1 (MQ=255)
                                                                          accAGAAATTTTATTTATAATTGTAGGACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAg                                                                 <  2:66820/140‑1 (MQ=255)
                                                                               aaaTTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACt                                                            >  2:170246/1‑141 (MQ=255)
                                                                                 aTTTTCTTTAAAATTGTAACACTATATCTTAAGCTGTAGAATTACTATAAAACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACttt                                                          <  2:110513/141‑1 (MQ=255)
                                                                                  ttttCTTTATAATTGTGGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTATCAAGACACtaaa                                                         >  2:194734/1‑138 (MQ=255)
                                                                                  ttgtatttATGATTTAAGCACTAATTCTTAAGCTGTTTAAGTACTATAATACAAAAAAAAAATATACAGCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTa                                                         <  2:194630/136‑1 (MQ=255)
                                                                                      cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTa                                                     >  1:369903/1‑141 (MQ=255)
                                                                                      cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACATTAAGTa                                                     >  1:339962/1‑141 (MQ=255)
                                                                                      cTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTAAAGAAACTTTAAGTa                                                     >  2:56348/1‑141 (MQ=255)
                                                                                       tttATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaa                                                    >  2:26212/1‑141 (MQ=255)
                                                                                       tttATAATTGTAGCAATATTTCTTATGCTGTTTAATTACTATAATACAAATAAAAAATATACAGCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaa                                                    <  2:277798/141‑1 (MQ=255)
                                                                                        ttATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTaaa                                                   <  1:282720/141‑1 (MQ=255)
                                                                                         tataAATGAAGTACTATATAATATACAGTATAATTACTAAACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAAAAAAAAAAAAAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAg                                                  <  2:22588/141‑1 (MQ=255)
                                                                                              ttGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGAAATTTCTCAAGAAACTTTAAGTAAAGAATTa                                             >  2:56492/1‑141 (MQ=255)
                                                                                                gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                           >  1:119426/1‑141 (MQ=255)
                                                                                                gTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaa                                           >  1:412944/1‑141 (MQ=255)
                                                                                                  agaaCTAATTCTTAAGCTGTTTAATTAATATACTAAAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAg                                         <  2:232029/138‑1 (MQ=255)
                                                                                                  aGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTaaaaa                                         >  2:302791/1‑140 (MQ=255)
                                                                                                  aGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAg                                         >  1:307237/1‑141 (MQ=255)
                                                                                                     aatATTTCTTATGATGTTTAATTACTATACTACAAATAAAAAATATAAATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAgtgt                                      <  2:295878/139‑1 (MQ=255)
                                                                                                      ttATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTc                                     <  1:309859/140‑1 (MQ=255)
                                                                                                       tATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCt                                    >  2:299895/1‑141 (MQ=255)
                                                                                                           tCTTATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGc                                <  1:245428/141‑1 (MQ=255)
                                                                                                             ttATGCTGTTTAATTACTATACTACAAATAAAAAATATACATCTAAAGATTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCaa                              >  2:317239/1‑141 (MQ=255)
                                                                                                               aTGCTGTTTAAATACTAGACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAAc                            <  1:47252/141‑1 (MQ=255)
                                                                                                                  atGTTTAATTACTATACTACAAAAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTAt                         <  1:271090/140‑1 (MQ=255)
                                                                                                                      ttAATTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAAt                     >  2:69487/1‑141 (MQ=255)
                                                                                                                        aaTTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAACAATTAAAAGTATCTAGGCAAACTATCAATGc                   >  1:416590/1‑141 (MQ=255)
                                                                                                                         aTTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCa                  >  2:166483/1‑141 (MQ=255)
                                                                                                                         aTTACTATACTACAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCa                  >  2:41551/1‑141 (MQ=255)
                                                                                                                            actatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa               >  2:258911/1‑141 (MQ=255)
                                                                                                                            actatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTATAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa               >  2:388280/1‑141 (MQ=255)
                                                                                                                            actatactaCAAATAAAAAATATACAACTAAAAGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATa               <  1:17860/141‑1 (MQ=255)
                                                                                                                             ctatactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAg              <  1:174129/141‑1 (MQ=255)
                                                                                                                               atactaCAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGaa            <  1:364899/141‑1 (MQ=255)
                                                                                                                                 actacAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTCTTAATGCAATAGaaaa          >  2:42757/1‑141 (MQ=255)
                                                                                                                                  ctacAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAAc         <  1:318623/141‑1 (MQ=255)
                                                                                                                                  atacAAATAAAAAATATACATCTAAAAATTAAATTAATGAAAAATATAATTAAATAATGTAGAAAAATCCACAAAACTGCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAAc         <  2:216468/140‑1 (MQ=255)
                                                                                                                                    acAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaa       <  2:368826/141‑1 (MQ=255)
                                                                                                                                     cAAATAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaat      <  1:45307/141‑1 (MQ=255)
                                                                                                                                          aaaaaaaTATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACaataaat  <  1:321641/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TGTATGTTATTTACACTAGTGCTTAAATTTACAGGTTTGTCACCAAGTATTGAAGGATCTTGGTTTTCTCAATATCCAGAAATTTTCTTTATAATTGTAGCACTATTTCTTATGCTGTTTAATTACTATACTACAAATAAAAAAATATACATCTAAAGGTTAAATTAATGAAAAATAGAATTAGAGAATATAGAAAACAAAAAGGAATTTCTCAAGAAACTTTAAGTAAAGAATTAAAAGTGTCTAGGCAAACTATTAATGCAATAGAAAACAATAAAT  >  CP000731/25525‑25803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: