Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,464 G→A 11.2% S1009S (TCG→TCA)  sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4640GA11.2% 201.5 / 5.1 100S1009S (TCG→TCA) sdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base G (29/55);  new base A (7/5);  total (36/64)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 101 total aligned reads displayed.

AGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622326‑622604
                                                                                                                                           |                                                                                                                                             
aggcagTGATTCTGATTCAGAGAGCGACACAGATTCAGATAGCGACACA‑ACTTGGAGTTAGATCTGAGACTCAGCGGTCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                             <  2:220884/138‑1 (MQ=2)
agacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAACCGGTCATTGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                             <  1:751838/141‑1 (MQ=17)
           cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGAGTCAGATAGCGATGCAGACTCAGAGAGCGACACAGATTCAGAAAGCCAGTCGGAGTCAGagcgcg                                                                                                                                  >  2:343150/1‑136 (MQ=14)
             gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAC‑TCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCCGACAGCGACTCAGACACAGAAAACGATACAGACCCacaccacgaa                                                                                                                                >  2:476470/1‑135 (MQ=11)
              attcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAtt                                                                                                                               <  1:586629/141‑1 (MQ=255)
                   gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑c                                                                                                                         <  1:125487/141‑1 (MQ=255)
                   gaCAGCGACTCAGATTAAGATAGAGACTAAGATTCGGACAGCGAT‑TCAGACTGAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑c                                                                                                                         <  2:716602/141‑1 (MQ=255)
                     cAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTc                                                                                                                       <  1:625196/141‑1 (MQ=255)
                      aGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAC‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACACAGATAGCGACTCAGATTCAGAGAGCGATTCGGCCTCAGATAGCGACTCAGA‑GTCa                                                                                                                      >  2:681030/1‑141 (MQ=11)
                         gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCGGAt                                                                                                                   <  1:994272/141‑1 (MQ=255)
                           tccacattcagatGGCGACTCAGATGCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg                                                                                                                 <  2:857868/136‑1 (MQ=255)
                           gccagatgcagaaAGCGAAGCAGATTCAGACAGCGAC‑GGAGATTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg                                                                                                                 <  2:336126/139‑1 (MQ=11)
                           cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCATATTCATATAGCGATTCAGACTCAGACAGCGACTCAGGTTTAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg                                                                                                                 <  1:268216/141‑1 (MQ=255)
                           cTCAGATTCAGATAGCGACTCAGATTCAGACAGCGAT‑TCAGTCTCAGATAGCGACGCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg                                                                                                                 <  1:482098/141‑1 (MQ=255)
                             cagattcagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCg                                                                                                               >  1:42712/1‑141 (MQ=255)
                               gattcagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgac                                                                                                             <  1:317524/141‑1 (MQ=255)
                                 ttcagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactc                                                                                                           <  1:793090/141‑1 (MQ=255)
                                 ttcagatAGCGACGCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactc                                                                                                           <  1:476170/141‑1 (MQ=255)
                                   cagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcag                                                                                                         >  1:599871/1‑141 (MQ=255)
                                   cagatACCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcag                                                                                                         <  1:221916/141‑1 (MQ=255)
                                     gatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagac                                                                                                       <  1:985035/141‑1 (MQ=255)
                                      atAGCGACTCAGAATCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagact                                                                                                      <  1:744533/141‑1 (MQ=255)
                                        aGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca                                                                                                    <  1:392753/141‑1 (MQ=255)
                                        aGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca                                                                                                    <  1:391908/141‑1 (MQ=255)
                                        aGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca                                                                                                    <  1:680143/141‑1 (MQ=255)
                                         gCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACACAGACAGCGATACAGA‑GTCCGACAGCGacgcagactcag                                                                                                   >  2:523573/1‑141 (MQ=11)
                                         gCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATACAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATGCGGACTCAGACAGCGATTCAGA‑CTCAGAGAGCGacccagacccag                                                                                                   >  2:882055/1‑141 (MQ=14)
                                             aacAGATGCTGACGGCGAT‑GCAGATGCAGATAGCGACGTAGATGCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAg                                                                                               <  2:325433/139‑1 (MQ=14)
                                              tCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATACCGACTCAGACTcagccagc                                                                                              >  2:312658/1‑141 (MQ=14)
                                              acAGATGCGGACAGCGAG‑GCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGc                                                                                              <  2:162443/140‑1 (MQ=255)
                                               cAGATTCGGACAGCGAT‑CCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGACAGCGCTTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCg                                                                                             <  1:201055/141‑1 (MQ=255)
                                                aaagatagACATCGAA‑TGAGACTCAGGTAGCGACTCAGATTAAGATATCGAGTGAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGa                                                                                            <  2:454837/134‑1 (MQ=14)
                                                 gcttCGGACAGCGAC‑TCCGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                           <  2:649424/139‑1 (MQ=255)
                                                 gactCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                           <  1:331411/138‑1 (MQ=255)
                                                 gATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATCGCGCCTCGGACTCAGACAGCGAc                                                                                           >  1:685676/1‑141 (MQ=255)
                                                 gATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                           <  1:716282/141‑1 (MQ=255)
                                                 gATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc                                                                                           <  1:736508/141‑1 (MQ=255)
                                                  atccagACAGCGAC‑TCAGACGCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACt                                                                                          <  2:867018/136‑1 (MQ=14)
                                                   ttCAGACAGCCAT‑TTATACTGAGATAGAGACTACGATGCAGACAGCGAATCAGAGTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtc                                                                                         <  2:676338/141‑1 (MQ=11)
                                                     cGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                       >  1:367886/1‑141 (MQ=255)
                                                     cGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcag                                                                                       <  1:565684/141‑1 (MQ=255)
                                                      ggAGAGCTAC‑TCATACTCATAAAGCGACGCTGTGTCTGACATCGTTTCATACTCTGACAGCTACTCGGATTCAGATAGCGATTCCGACTCAGACAATG‑TTAATACCGCTTATAGCGACTCAGACTCAGACAGCGACtcaga                                                                                      <  2:470845/141‑1 (MQ=9)
                                                           gCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCCGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                 >  2:118877/1‑141 (MQ=255)
                                                                   ggACTCAGACAGTGACTCGGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGA‑TTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa                                                                          <  2:1023471/140‑1 (MQ=11)
                                                                    gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACACAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                         >  1:950053/1‑141 (MQ=255)
                                                                     aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGaat                                                                        >  1:442021/1‑139 (MQ=255)
                                                                     aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAtt                                                                        >  1:768438/1‑141 (MQ=255)
                                                                       tCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg                                                                      >  1:72218/1‑141 (MQ=255)
                                                                       tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAACGACTCACACTCAGACAGCGACTAAGATGACGATAGCGACTcc                                                                      >  2:853115/1‑140 (MQ=14)
                                                                       tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGAATca                                                                      >  2:961811/1‑140 (MQ=255)
                                                                        aaGATAGCGACTCAGAGTCAGAGAGCGATTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                     <  1:382277/140‑1 (MQ=255)
                                                                             aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATAACGACTCAGACACAAACAACGACTCAGACTCAGAAACCAACTCGGACtaa                                                                >  2:365144/1‑139 (MQ=11)
                                                                               cGACTCAGATTCAGATAGCGAGTAAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCACATCCACATAGCAACTCACACTCaaa                                                              >  2:751029/1‑139 (MQ=11)
                                                                                gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGCATCACACTCTGACAGCGACTCGGCCTCacaa                                                             >  2:60217/1‑138 (MQ=11)
                                                                                   tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                          <  1:553723/141‑1 (MQ=255)
                                                                                    cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                         >  1:931525/1‑141 (MQ=255)
                                                                                    cagattaagatAGCGATTCAGACTCAGACAGCGACTCAGATGCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                         <  1:132891/141‑1 (MQ=255)
                                                                                     agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCCGACAGCGa                                                        >  2:337617/1‑141 (MQ=11)
                                                                                          cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGATACAGACTCAGACAGCGACTCaa                                                   >  2:885428/1‑140 (MQ=11)
                                                                                          cagagAGGGATTCAGCATCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                   <  2:42712/141‑1 (MQ=14)
                                                                                           agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGa                                                  <  1:687000/141‑1 (MQ=255)
                                                                                            gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGaa                                                 >  2:754532/1‑140 (MQ=14)
                                                                                               agggaatcagactgagatAGAGACTGATATTAAGACAGCGATAGGGAATCAGAGAGCGAGTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCa                                              <  2:284120/135‑1 (MQ=14)
                                                                                               aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGCCTCAGacaca                                              >  2:333866/1‑137 (MQ=14)
                                                                                                   aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACa                                          <  1:735621/141‑1 (MQ=255)
                                                                                                    ttCAGTCCCAGACCGCGACTCAGCTTCAGATAGCGATTCGGACTCAGACAGCGAGTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                         <  2:599871/141‑1 (MQ=14)
                                                                                                       ggactcagacACAGCCTCAGATTCAGAGAGCGATTCTGACTCAGACGGGGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa                                      <  2:830757/140‑1 (MQ=14)
                                                                                                        gactcaggtCGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                     <  2:148919/141‑1 (MQ=255)
                                                                                                        gacgcagaaGGCGACTACGATTCAGACAGCGCTTCAGACTCAGAAAGCGACTCAGA‑CTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                     <  2:157069/141‑1 (MQ=11)
                                                                                                         actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                    >  1:557388/1‑141 (MQ=255)
                                                                                                         actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTTAGATAGCGACTCAGAGTCGGTCAGCGagt                                    >  1:674402/1‑139 (MQ=14)
                                                                                                           ccagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa                                  <  1:950273/140‑1 (MQ=255)
                                                                                                             aaacAGCGACTCAGATTCAGATAGCGACTCGGACTCAGAGAGCGACTCAGA‑CTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa                                <  2:177145/139‑1 (MQ=255)
                                                                                                              gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                               <  1:939196/141‑1 (MQ=255)
                                                                                                              gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                               <  1:882055/141‑1 (MQ=255)
                                                                                                                cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                             <  1:343150/141‑1 (MQ=255)
                                                                                                                    gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAAACCAAAGACTAAATCGTTTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                         >  1:751545/1‑141 (MQ=17)
                                                                                                                      cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGCTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                       <  1:541075/141‑1 (MQ=255)
                                                                                                                      cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                       <  1:814072/141‑1 (MQ=255)
                                                                                                                      cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                       <  1:559104/141‑1 (MQ=255)
                                                                                                                      cTCAGATTCAAATAGCAATTCGGACTCAGACAGCGATTCCGA‑CTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                       <  1:968881/141‑1 (MQ=255)
                                                                                                                         agattcagatATCTATTCGGCCTCAGACGCCGATTCAGA‑CTCAGATAGCGCCTCAGACTCAGTCACCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga                    <  1:285019/141‑1 (MQ=17)
                                                                                                                          gatgcagacAGCGACGCCGACGCAGACAGCGATTCCGA‑GTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                   <  2:472632/141‑1 (MQ=14)
                                                                                                                            ttcatatCGCGATTCGGACTCAGACGGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                 <  1:493088/141‑1 (MQ=255)
                                                                                                                            ttcagacAACGAGGCAGACACAGACAGCGATTCAGA‑CACAGATAGCGACTCAGAGTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                 <  2:454050/141‑1 (MQ=11)
                                                                                                                              cagatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTAAGACTCAGATAGCgactcag               >  1:868960/1‑141 (MQ=255)
                                                                                                                                gatGGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac             <  1:70248/141‑1 (MQ=255)
                                                                                                                                gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac             <  1:392453/141‑1 (MQ=255)
                                                                                                                                gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAAACAGCGAATCATGCTCTGATAGCcactcagac             >  1:139357/1‑141 (MQ=17)
                                                                                                                                gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCCGAGCGCgactcacac             >  2:244965/1‑141 (MQ=14)
                                                                                                                                  ttgagATTCCGGCTCAGACAGCGACCCAGA‑CTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCGGGCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAAAGATCtactcagactc           <  2:376887/137‑1 (MQ=11)
                                                                                                                                  tAGAGATGCGGGCTAAGACGGCGACCCAGA‑CGCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc           <  2:804897/141‑1 (MQ=11)
                                                                                                                                  caGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc           <  1:515599/140‑1 (MQ=255)
                                                                                                                                  aaGCGAGTCGGACTCAGACAGGGAGTGAGA‑CTCAGATAGCGACTCACACCCAGAAAGACACTCAGACTCAGACAGCGACTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc           <  2:592160/140‑1 (MQ=11)
                                                                                                                                        ttCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAACCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCACACTACGATAGCGCCTCAGACCCAGACAg     >  2:559824/1‑141 (MQ=14)
                                                                                                                                         tCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt    <  1:289001/141‑1 (MQ=255)
                                                                                                                                          cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg   >  1:683242/1‑141 (MQ=255)
                                                                                                                                          cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg   >  1:626692/1‑141 (MQ=255)
                                                                                                                                          cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGTg   >  1:728459/1‑141 (MQ=255)
                                                                                                                                           ggACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTga  >  2:681404/1‑141 (MQ=255)
                                                                                                                                           |                                                                                                                                             
AGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA  >  USA300TCH1516_ALE/622326‑622604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑