Predicted mutation
evidence seq id position mutation annotation gene description
RA CP000731 81 N→G intergenic (–/‑151) – / → repA –/replication protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000731810NG95.9% 138.2 / NA 50intergenic (–/‑151)–/repA–/replication protein A
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base G (29/18);  minor base A (0/2);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAG  >  CP000731/1‑219
                                                                                                                                         |                                                                                                                                          
tatttatcaagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGaa                                                                                                                                          >  1:14532‑M1/58‑140 (MQ=255)
       caagggtttataaacccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTAttt                                                                                                                                   <  2:149243‑M1/90‑1 (MQ=255)
                    acccttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAAttt                                                                                                                      >  2:12991‑M1/38‑140 (MQ=255)
                       cttgataaagctatattttcccttttataagtttGTTTAAATTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCg                                                                                                                   >  2:65872‑M1/35‑140 (MQ=255)
                       cttgataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAAAAACTGTTTATTTATATAATTTTCg                                                                                                                   <  1:215940‑M1/106‑1 (MQ=255)
                          gataaagctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATa                                                                                                                >  1:55795‑M1/32‑140 (MQ=255)
                               agctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATacttat                                                                                                           >  1:51446‑M1/27‑138 (MQ=255)
                               agctatattttcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGt                                                                                                           >  1:39184‑M1/27‑140 (MQ=255)
                                 ctatattttcccttttataagtttATTTAAAGTGCTATCATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGtat                                                                                                         >  1:125632‑M1/25‑140 (MQ=255)
                                         tcccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAAt                                                                                                 <  1:180471‑M1/124‑1 (MQ=255)
                                          cccttttataagtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATAATTGTATTATATAATg                                                                                                <  1:94710‑M1/125‑1 (MQ=255)
                                                     gtttATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTAGATACTTGTATTATATAATGAATAATAACtt                                                                                     <  2:70364‑M1/136‑1 (MQ=255)
                                                         aTTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTg                                                                                 <  1:175764/140‑1 (MQ=255)
                                                             aaaGTGCTATAATAAATTTAATATAACAAAAACCCCAACTATTGCGAATAGTCGGGGTTTGGTAATAAAACGTTTAAAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAg                                                                             <  2:248320/140‑1 (MQ=255)
                                                             aaaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGCAACTGTTTATGTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAg                                                                             <  1:100741/140‑1 (MQ=255)
                                                              aaGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAgt                                                                            >  2:262877/1‑139 (MQ=255)
                                                               aGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAATCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCAATACTTTTATTATATAATAAATAATATTTTAATGCCAgaa                                                                           >  2:148520/1‑138 (MQ=255)
                                                               aGTGCTATAATAAACTTAATATAACAAAAACCACAACTACTGGTAATAGTAGGGGTTTGGTAATAAAAAGTTTAGAAAATGTTTATTTATATAATTTTCGATATTTGTATTGTATAATGAATAATATTTTAGTGCAAGCa                                                                           >  2:150273/1‑140 (MQ=255)
                                                                gTGCTATAATAAACTTAATATAACAAAAACCCCCACTACTGGTAATACTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAc                                                                          <  1:181774/140‑1 (MQ=255)
                                                                 tGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATCATCGCCTTACCCAACGCCTCATATCTTAGTGCAAGCAcc                                                                         <  1:63442/140‑1 (MQ=255)
                                                                 tGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCAcc                                                                         <  1:144050/140‑1 (MQ=255)
                                                                         taaACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCAt                                                                 <  1:125061/140‑1 (MQ=255)
                                                                            aCTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATAAATAATAACTTAGTGCAAGCACCTAAATCATtaa                                                              >  1:84999/1‑140 (MQ=255)
                                                                                aaTATAAAAAAAACGCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATtaaataa                                                          <  2:140513/140‑1 (MQ=255)
                                                                                      aCAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTaa                                                    >  1:174454/1‑140 (MQ=255)
                                                                                         aaacCCCCCACCACCGGTAATACGCGGGGTTTGGTAAAAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGc                                                 <  1:57894/140‑1 (MQ=255)
                                                                                          aaaCCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCt                                                >  2:14365/1‑140 (MQ=255)
                                                                                              cccAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                            >  2:181262/1‑140 (MQ=255)
                                                                                              caaaaCTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaat                                            <  2:61175/137‑1 (MQ=255)
                                                                                               tcAACTACTGGTAATAGTTGGGGTTTGGTAATAAAACGTTTATAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAACGACTGATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                           <  2:187636/139‑1 (MQ=255)
                                                                                               tcAAATACTGGTAATAGTCGGGGTTTGGTAATAAATCGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaata                                           <  2:235345/139‑1 (MQ=255)
                                                                                                cAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATATATGTTAAGCtaaataa                                          >  1:98799/1‑140 (MQ=255)
                                                                                                cAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCtaaataa                                          >  1:20658/1‑140 (MQ=255)
                                                                                                cAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGAACCTAAATCATTAAATAAATGTAAAGCtaaataa                                          >  1:236720/1‑140 (MQ=255)
                                                                                                        tgTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGttt                                  <  1:17329/139‑1 (MQ=255)
                                                                                                        ggTAATAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGACTAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGttt                                  >  1:65296/1‑140 (MQ=255)
                                                                                                           aaTAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTttat                               >  1:172479/1‑140 (MQ=255)
                                                                                                             tAGTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATATTAGAGAAAGCACCTAAATCATTAAATAAATATAATACTAAATAAAAACGCTtgatta                             >  2:160680/1‑140 (MQ=255)
                                                                                                               gTCGGGGTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATCAcc                           >  2:138521/1‑140 (MQ=255)
                                                                                                                  ggggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACctct                        >  1:187963/1‑140 (MQ=255)
                                                                                                                    ggTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTa                      >  2:134389/1‑140 (MQ=255)
                                                                                                                     gTTTGGTAATAAAACGTTTAGATACTGTTTTGTTTTAGAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAc                     >  1:194853/1‑140 (MQ=255)
                                                                                                                     gTTTGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACTTCTTAc                     >  1:174693/1‑140 (MQ=255)
                                                                                                                      tttGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTAcc                    <  1:43723/140‑1 (MQ=255)
                                                                                                                      tttGGTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATAATTAAATAAATGTAAAGCTAAATAAAAACGATTTATTACCTCTTAcc                    >  2:69247/1‑140 (MQ=255)
                                                                                                                         ggTAATAGACCATTTAGAAACGGTTTATTTATATAATTTTAGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa                 <  2:98849/140‑1 (MQ=255)
                                                                                                                         ggTAATAAAACGTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCaaa                 >  1:215599/1‑140 (MQ=255)
                                                                                                                                    gTTTAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTCCATCTTACCAAACTAAAACATgg      >  2:166672/1‑140 (MQ=255)
                                                                                                                                     tttAGAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGa     >  1:182005/1‑140 (MQ=255)
                                                                                                                                        aGAAACTGTTTATTTATATAATTTTCCATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAag  <  2:41967/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATTTAAAGTGCTATAATAAACTTAATATAACAAAAACCCCAACTACTGGTAATAGTCGGGGTTTGGTAATAAAACGTTTANAAACTGTTTATTTATATAATTTTCGATACTTGTATTATATAATGAATAATATCTTAGTGCAAGCACCTAAATCATTAAATAAATGTAAAGCTAAATAAAAACGTTTTATTACCTCTTACCAAACTAAAATATGGAAAG  >  CP000731/1‑219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑