Predicted mutation
evidence seq id position mutation annotation gene description
RA USA300TCH1516_ALE 774,414 G→A G555D (GGT→GAT)  fruA → PTS system fructose‑specific EIIABC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE774,4140GA98.3% 398.9 / ‑2.0 117G555D (GGT→GAT) fruAPTS system fructose‑specific EIIABC component
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (51/64);  total (52/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01

Only 100 of 120 total aligned reads displayed.

AGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCACATG  >  USA300TCH1516_ALE/774277‑774551
                                                                                                                                         |                                                                                                                                         
aGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGatat                                                                                                                                        >  4:53879/1‑141 (MQ=255)
  gTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGt                                                                                                                                      <  2:535791/141‑1 (MQ=255)
    aaTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCa                                                                                                                                    >  4:126063/1‑141 (MQ=255)
     aTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCAt                                                                                                                                   >  2:663262/1‑141 (MQ=255)
     aTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCAt                                                                                                                                   >  4:266703/1‑141 (MQ=255)
     aTGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCAt                                                                                                                                   >  1:634167/1‑141 (MQ=255)
         agcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttat                                                                                                                               <  2:599762/141‑1 (MQ=255)
          gcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttatt                                                                                                                              >  2:235640/1‑141 (MQ=255)
          gcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttatt                                                                                                                              >  3:7576/1‑141 (MQ=255)
          gcaCCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATttatt                                                                                                                              >  4:108147/1‑141 (MQ=255)
             ccAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACa                                                                                                                           >  1:696813/1‑141 (MQ=255)
             ccAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACa                                                                                                                           >  1:639822/1‑141 (MQ=255)
             ccAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACa                                                                                                                           >  3:208937/1‑141 (MQ=255)
              cAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAg                                                                                                                          >  2:22288/1‑141 (MQ=255)
                aTTACATCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGaa                                                                                                                        >  4:285784/1‑141 (MQ=255)
                  tACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAgg                                                                                                                      <  2:225542/141‑1 (MQ=255)
                    cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTg                                                                                                                    >  2:537566/1‑141 (MQ=255)
                    cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTg                                                                                                                    >  1:107455/1‑141 (MQ=255)
                    cAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATCTATTACAGAAGGTg                                                                                                                    >  1:492262/1‑141 (MQ=255)
                      ggtGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCg                                                                                                                  <  4:159822/139‑1 (MQ=255)
                      gCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCg                                                                                                                  <  1:484836/141‑1 (MQ=255)
                      gCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCg                                                                                                                  <  1:33691/141‑1 (MQ=255)
                        tGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGAt                                                                                                                >  1:152936/1‑141 (MQ=255)
                              gATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCAtt                                                                                                          <  2:469877/141‑1 (MQ=255)
                                    tggtATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGc                                                                                                    >  4:302280/1‑141 (MQ=255)
                                      gtATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTg                                                                                                  <  3:91020/141‑1 (MQ=255)
                                        aTGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTg                                                                                                  <  2:711849/139‑1 (MQ=255)
                                        aTGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTg                                                                                                  >  1:711849/1‑139 (MQ=255)
                                         tGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCg                                                                                               <  1:25791/141‑1 (MQ=255)
                                         tGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCg                                                                                               <  2:92444/141‑1 (MQ=255)
                                           aTTCCACCGTTAGCAATTGCGACAGCGCTGTTAACTTTTAGACGTAAATTTACACAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCAATTTGCACATGCCGAt                                                                                             >  2:667459/1‑141 (MQ=255)
                                           aTTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGAt                                                                                             <  1:291988/141‑1 (MQ=255)
                                           aTTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGAt                                                                                             <  2:639822/141‑1 (MQ=255)
                                           aTTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTACATTTGCAGCTGCCGAt                                                                                             >  2:667447/1‑141 (MQ=255)
                                            ttCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATc                                                                                            >  3:189803/1‑141 (MQ=255)
                                            ttCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATc                                                                                            >  3:321331/1‑141 (MQ=255)
                                                aCCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCAtt                                                                                        >  2:273342/1‑141 (MQ=255)
                                                  cGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTAc                                                                                      <  1:38555/141‑1 (MQ=255)
                                                   gTTAGCAATTGCGACAGCGATGTTAATTTTTAGTCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACg                                                                                     <  4:263561/141‑1 (MQ=255)
                                                     tAGCACTTGCGACAGCGATGTTGATTTTTAGACGTAAATTTACAAAAGAACAGCGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACgtg                                                                                   <  1:721090/141‑1 (MQ=255)
                                                        cAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTa                                                                                <  4:61953/141‑1 (MQ=255)
                                                         aaTTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTAt                                                                               <  1:366368/141‑1 (MQ=255)
                                                         aaTTGCGACAGCGATGTTAATTTTTAGACGTAAATATACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTAt                                                                               >  2:189733/1‑141 (MQ=255)
                                                           ttGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTTAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTc                                                                             <  1:251753/141‑1 (MQ=255)
                                                           ttGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTc                                                                             <  3:123068/141‑1 (MQ=255)
                                                               gACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTc                                                                         <  1:683365/141‑1 (MQ=255)
                                                               gACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTc                                                                         <  3:204995/141‑1 (MQ=255)
                                                                aCAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCa                                                                        <  4:264464/141‑1 (MQ=255)
                                                                aCAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCa                                                                        <  2:1650/141‑1 (MQ=255)
                                                                 cAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACTACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCaa                                                                       <  2:376937/141‑1 (MQ=255)
                                                                 cAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCaa                                                                       <  3:53879/141‑1 (MQ=255)
                                                                   gcaaTGTTATATTTTAGTCGTAAATTTACAAAAGAACTACGTGGTTCAATTGTCCCTTACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCGATTTGCAGCTTACGATCCATTACGTGTTATTCCTTcgctg                                                                     <  2:152936/138‑5 (MQ=255)
                                                                   gCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCAGCCGATCCATTACGTGTTATTCCTTCAatg                                                                     <  2:711151/141‑1 (MQ=255)
                                                                    cGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAatga                                                                    >  3:321262/1‑141 (MQ=255)
                                                                       tGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAAtgatga                                                                 <  2:107455/141‑1 (MQ=255)
                                                                       tGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAAtgatga                                                                 <  3:108147/141‑1 (MQ=255)
                                                                       tGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAAtgatga                                                                 <  1:580966/141‑1 (MQ=255)
                                                                        gTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATgatgat                                                                <  1:448895/141‑1 (MQ=255)
                                                                        gTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATgatgat                                                                <  3:126063/141‑1 (MQ=255)
                                                                          tAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTg                                                              >  1:664242/1‑141 (MQ=255)
                                                                           aaTTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTgg                                                             >  4:149464/1‑141 (MQ=255)
                                                                            aTTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGt                                                            <  1:657010/141‑1 (MQ=255)
                                                                             tttttAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGtt                                                           <  2:171911/141‑1 (MQ=255)
                                                                              ttttAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTc                                                          >  1:568586/1‑141 (MQ=255)
                                                                                ttAGACGTAAATTTACAAAAGAACAACGTGGTTCCATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAg                                                        <  1:730974/141‑1 (MQ=255)
                                                                                    aCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGtat                                                    <  1:200969/141‑1 (MQ=255)
                                                                                    aCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGtat                                                    >  2:366024/1‑141 (MQ=255)
                                                                                    aCGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGtat                                                    >  1:488824/1‑141 (MQ=255)
                                                                                             tACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTa                                                                                <  4:261217/104‑1 (MQ=255)
                                                                                             tACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTa                                                                                >  3:261217/1‑104 (MQ=255)
                                                                                              aCAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCa                                          <  3:285784/141‑1 (MQ=255)
                                                                                               cAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTcc                                                                            >  4:2698/1‑106 (MQ=255)
                                                                                                  aaGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTg                                      <  2:534372/141‑1 (MQ=255)
                                                                                                    gAACAACGTGGTTCAATTATTCCAAACTAGGTGAGGGAGATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTACAATGAAGAGTGGTTCAGGTATAGGTGGCGCAATTGCt                                    >  2:201508/1‑141 (MQ=255)
                                                                                                     aacaacGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCtt                                   <  2:696568/141‑1 (MQ=255)
                                                                                                     aacaacGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCtt                                   <  1:153578/141‑1 (MQ=255)
                                                                                                         acGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGTTATAGGTGGCGCAATTGCTTTAgg                               >  4:226892/1‑141 (MQ=255)
                                                                                                         acGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAgg                               >  4:58312/1‑141 (MQ=255)
                                                                                                         acGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAgg                               >  2:572958/1‑141 (MQ=255)
                                                                                                          cGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTTCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGc                              <  1:122207/141‑1 (MQ=255)
                                                                                                          cGTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGc                              <  1:663262/141‑1 (MQ=255)
                                                                                                           gTGGTTCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCt                             <  3:149464/141‑1 (MQ=255)
                                                                                                                tCAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGt                        >  1:559920/1‑141 (MQ=255)
                                                                                                                 cAATTATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGtt                       <  2:122823/141‑1 (MQ=255)
                                                                                                                     tATCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACg                   <  2:437436/141‑1 (MQ=255)
                                                                                                                       tCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGaa                 <  1:263963/141‑1 (MQ=255)
                                                                                                                       tCCCTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGaa                 <  2:186987/141‑1 (MQ=255)
                                                                                                                        cccTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTTCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAAt                <  4:321331/141‑1 (MQ=255)
                                                                                                                        cccTAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAAt                <  2:764139/141‑1 (MQ=255)
                                                                                                                           tAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGc                                                                                                 >  3:21584/1‑57 (MQ=255)
                                                                                                                           tAACTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGc                                                                                                 <  4:21584/57‑1 (MQ=255)
                                                                                                                              cTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTATGCTTAGGTTCACGAATTACTgc          >  3:12129/1‑141 (MQ=255)
                                                                                                                              cTATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgc          <  2:229488/141‑1 (MQ=255)
                                                                                                                               tATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgcg         <  1:273342/141‑1 (MQ=255)
                                                                                                                               tATGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgcg         <  1:337494/141‑1 (MQ=255)
                                                                                                                                aTGTGATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTgcgc        >  4:291533/1‑141 (MQ=255)
                                                                                                                                   tgATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCcac     <  1:36145/141‑1 (MQ=255)
                                                                                                                                   tgATGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGGTCACGAATTACTGCGCcac     >  1:524843/1‑141 (MQ=255)
                                                                                                                                   ggATGGATATCTCATTTATTACACAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCcac     <  4:270098/140‑1 (MQ=255)
                                                                                                                                      tGGATATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCACAtg  <  2:314001/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AGGTAATGCAGCACCAATTACAGCTGCAATGATTGGTGGTATGATTCCACCGTTAGCAATTGCGACAGCGATGTTAATTTTTAGACGTAAATTTACAAAAGAACAACGTGGTTCAATTATCCCTAACTATGTGATGGGTATGTCATTTATTACAGAAGGTGCGATTCCATTTGCAGCTGCCGATCCATTACGTGTTATTCCTTCAATGATGATTGGTTCAGGTATAGGTGGCGCAATTGCTTTAGGCTTAGGTTCACGAATTACTGCGCCACATG  >  USA300TCH1516_ALE/774277‑774551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: