New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? USA300TCH1516_ALE 329202 =129 (1.360)7 (0.080) 6/246 10.1 5.9% intergenic (+70/+73) USA300HOU_RS01475/ssaA2_1 hypothetical protein/Staphylococcal secretory antigen ssaA2
?USA300TCH1516_ALE 329255 = 103 (1.180)intergenic (+123/+20) USA300HOU_RS01475/ssaA2_1 hypothetical protein/Staphylococcal secretory antigen ssaA2
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGGATTGGTGAATATATCGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/329349‑329202
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggtcgctttTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCAATCCAGTTCATTTCTGAAATTAGAATACTTCCATCAATATTAACTTTT  >  USA300TCH1516_ALE/329255‑329392
                                                                                                                                                                                                                                                                                               
TGGATTGGTGAATATATCGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGG                                                                                                                                                    >  2:210049/1‑141
 GGATTGGTGAATATATCGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGT                                                                                                                                                   >  3:346031/1‑141
 GGATTGGTGAATATAACGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCACAATTGGT                                                                                                                                                   >  3:346041/1‑141
  GATTGGTGAATATATCGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTC                                                                                                                                                  >  3:153415/1‑141
      GGTGAATATATCGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTT                                                                                                                                              <  1:175954/141‑1
       GTGAATATATCGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATACGTAACACTGCAAAATTGGTCGCTTT                                                                                                                                             >  1:371254/1‑141
                                                                             TAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTTATTTTGTATGTTATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTT                                                                       >  2:137070/1‑141
                                                                                   TGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATAGGTCTCTTTATTTTGTATGTCATGATTAATTTAATGGATGTAATTATATGATGAACCTTCTGAAGCAAAGATGGTTTTTGATGAA                                                                 >  2:167020/1‑141
                                                                                                     ACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCACTCC                                              >  2:348002/1‑141
                                                                                                         GTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCAATCCAGTT                                          >  1:627562/1‑141
                                                                                                         GTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCAATCCAGTT                                          >  3:243947/1‑141
                                                                                                             GCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACG                                                              <  2:146161/117‑1
                                                                                                             GCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACG                                                              >  1:146161/1‑117
                                                                                                                                            GTCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCAATCCAGTTCATTTCTGAAATTAGAATACTTCCATCAATATTAA       >  3:191048/1‑141
                                                                                                                                            GTCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCAATCCAGTTCATTTCTGAAATTAGAATACTTCCATCAATATTAA       >  3:333815/1‑141
                                                                                                                                             TCGCTTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCAATCCAGTTCATTTCTGAAATTAGAATACTTCCATCAATATTAAC      <  4:6906/141‑1
                                                                                                                                                 TTTTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATAGACGCCAATCCAGTTCATTTCTGAAATTAGAATACTTCCATCAATATTAACTTTT  >  1:450444/1‑141
                                                                                                                                                                                                                                                                                               
TGGATTGGTGAATATATCGTTTCATCAAGAACCATCTCTGCTTCAGAAGTTTCATCATATAATTACATCCATTAAATTAATCATGACATCAATAAAAAGCGACCAGTTCGCAGTTTACAATTCGTAACACTGCAAAATTGGTCGCTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  USA300TCH1516_ALE/329349‑329202
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggtcgctttTTATTGATGTCATGATTAATTTAATGGATGTAATTATATGATGAAACTTCTGAAGCAGAGATGGTTCTTGATGAAACGATATATTCACCAATCCAGTTCATTTCTGAAATTAGAATACTTCCATCAATATTAACTTTT  >  USA300TCH1516_ALE/329255‑329392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.