BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,039,417	T→G	5.3%	L13V (TTA→GTA)	USA300TCH1516_00965 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,039,417	0	T	G	5.3%	359.9 / 11.5	113	L13V (TTA→GTA)	USA300TCH1516_00965	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (46/61); new base G (4/2); total (50/63)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 119 total aligned reads displayed.

ATGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAAGA  >  USA300TCH1516_ALE/1039277‑1039557

atgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAatat                                                                                                                                              <  1:612692/141‑1 (MQ=255)

atgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAatat                                                                                                                                              <  1:773658/141‑1 (MQ=255)

 tgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGCTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGCTTTAACTATGAGATTTATCATTGCAATATTATTAGGATTAAtatt                                                                                                                                             >  1:584285/1‑141 (MQ=255)

  gatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATGTGCAGCCTTTACTATCATGGCAGACG‑TTTAATTAAGTTATCATGCAGTGTTGACTAGGAAATTTATCATTGCAATATTATTAGGATTAAtattat                                                                                                                                           <  2:1084355/141‑1 (MQ=255)

     gTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATcc                                                                                                                                         >  2:183343/1‑141 (MQ=255)

        aTTTGCAACGATTCTTGTCGTTCACATATTATTGCAGATTTGCAGCCTATACTATCATGGCAGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCAtt                                                                                                                                      >  2:432441/1‑141 (MQ=255)

         tttGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTa                                                                                                                                     <  3:208796/141‑1 (MQ=255)

            gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                  <  4:284809/141‑1 (MQ=255)

            gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                  <  3:399444/141‑1 (MQ=255)

            gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                  <  2:707719/141‑1 (MQ=255)

               aCGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTg                                                                                                                               >  3:320186/1‑141 (MQ=255)

                  aTTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCtt                                                                                                                            <  1:639685/141‑1 (MQ=255)

                   ttCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGCTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCttt                                                                                                                           <  1:103628/141‑1 (MQ=255)

                    tCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCtttt                                                                                                                          <  2:590551/141‑1 (MQ=255)

                       tGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAGCGGTGAAATGTGTCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACa                                                                                                                       >  3:154385/1‑141 (MQ=255)

                       tGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACa                                                                                                                       >  4:188041/1‑141 (MQ=255)

                        gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCa                                                                                                                                        <  2:400903/123‑1 (MQ=255)

                        gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCa                                                                                                                                        >  1:400903/1‑123 (MQ=255)

                        gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACaa                                                                                                                      <  2:488342/141‑1 (MQ=255)

                           gTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGATGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCa                                                                                                                   <  1:688402/141‑1 (MQ=255)

                               aaaTATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAca                                                                                                               <  1:1073468/141‑1 (MQ=255)

                                      attGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCt                                                                                                        <  2:561602/141‑1 (MQ=255)

                                       ttGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCta                                                                                                       <  3:443679/141‑1 (MQ=255)

                                          cAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATAc                                                                                                    <  1:303419/141‑1 (MQ=255)

                                            gATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTCTACtt                                                                                                  >  2:420101/1‑141 (MQ=255)

                                             aTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTg                                                                                                 <  1:595067/141‑1 (MQ=255)

                                             aTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTAGCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTg                                                                                                 <  4:424197/141‑1 (MQ=255)

                                              tttGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGa                                                                                                <  2:198638/141‑1 (MQ=255)

                                                tGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACCATCATACATCATCCTATACTTGATc                                                                                              <  2:565167/141‑1 (MQ=255)

                                                tGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATc                                                                                              <  1:18220/141‑1 (MQ=255)

                                                 gCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCg                                                                                             >  1:873927/1‑141 (MQ=255)

                                                    gCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGttt                                                                                          >  2:346644/1‑141 (MQ=255)

                                                    gCCTATACTAGCATATGCGACGTTTTAATGATGTTATCATGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTTTCCTTGATCGttt                                                                                          <  4:258030/141‑1 (MQ=255)

                                                     ccTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGtttt                                                                                         <  1:876357/141‑1 (MQ=255)

                                                     ccTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGtttt                                                                                         <  3:337148/141‑1 (MQ=255)

                                                     ccTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGtttt                                                                                         <  1:519879/141‑1 (MQ=255)

                                                      cTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTa                                                                                        >  4:326832/1‑141 (MQ=255)

                                                        ataCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAAt                                                                                      >  1:465623/1‑141 (MQ=255)

                                                         taCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATc                                                                                     <  1:863350/141‑1 (MQ=255)

                                                          aCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATcc                                                                                    <  2:202306/141‑1 (MQ=255)

                                                             aTCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCttt                                                                                 >  1:79394/1‑141 (MQ=255)

                                                              tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                                >  4:380244/1‑141 (MQ=255)

                                                              tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                                >  1:271725/1‑141 (MQ=255)

                                                              tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                                >  1:202464/1‑141 (MQ=255)

                                                                aTGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCtt                                                                              <  1:502845/141‑1 (MQ=255)

                                                                aTGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCtt                                                                              >  2:941669/1‑141 (MQ=255)

                                                                   gCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAACTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaa                                                                           <  1:854621/141‑1 (MQ=255)

                                                                     cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATTCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                         <  1:562442/141‑1 (MQ=255)

                                                                     cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                         >  2:1017362/1‑141 (MQ=255)

                                                                     cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATCAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                         >  1:785696/1‑141 (MQ=255)

                                                                      gACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAAc                                                                        <  2:395554/141‑1 (MQ=255)

                                                                      gACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAAc                                                                        <  1:470197/141‑1 (MQ=255)

                                                                       aCGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACg                                                                       >  3:55614/1‑141 (MQ=255)

                                                                        cGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACgg                                                                      >  3:235503/1‑141 (MQ=255)

                                                                           tttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGt                                                                   <  2:7675/141‑1 (MQ=255)

                                                                            ttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGta                                                                  >  2:583650/1‑141 (MQ=255)

                                                                             tAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtat                                                                 >  2:147628/1‑141 (MQ=255)

                                                                              aaggAAGTTATCAGTAATTTTAAACTATTAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACAGCTTCCTATGCTTGAGCGTTTTAAACCTTTCTAAAAAACGGAGtata                                                                <  2:52248/138‑1 (MQ=255)

                                                                              aaTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                                >  1:351098/1‑141 (MQ=255)

                                                                              aaTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                                >  2:997527/1‑141 (MQ=255)

                                                                              aaTGAAGTTATCAAGGAGTTTTAAATATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAAATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                                <  2:130734/141‑1 (MQ=255)

                                                                               aTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAg                                                               >  1:75232/1‑141 (MQ=255)

                                                                                tGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                              <  1:608637/141‑1 (MQ=255)

                                                                                tGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                              <  2:183943/141‑1 (MQ=255)

                                                                                tGAAGTTATCAAGGAGTTCTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTCTACATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                              <  1:214460/141‑1 (MQ=255)

                                                                                  aaGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTa                                                            <  1:727815/141‑1 (MQ=255)

                                                                                   aGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTAt                                                           >  3:205789/1‑141 (MQ=255)

                                                                                     ttATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGc                                                         <  2:860193/141‑1 (MQ=255)

                                                                                       aTCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCa                                                       >  2:885037/1‑141 (MQ=255)

                                                                                        tCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCaa                                                      >  4:119994/1‑141 (MQ=255)

                                                                                          aaGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAg                                                    <  2:912639/141‑1 (MQ=255)

                                                                                             gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                 <  1:96402/141‑1 (MQ=255)

                                                                                             gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                 >  2:865938/1‑141 (MQ=255)

                                                                                             gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                 >  3:215444/1‑141 (MQ=255)

                                                                                                    aaCTATGAAATTTATCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAgg                                          >  1:603459/1‑141 (MQ=255)

                                                                                                     aCTATGAAATTTATCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGACAAAGAGTCAAAAGGt                                         >  2:585257/1‑141 (MQ=255)

                                                                                                      cTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTa                                        <  1:226629/141‑1 (MQ=255)

                                                                                                        aTGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACg                                      <  3:441318/141‑1 (MQ=255)

                                                                                                         tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                     >  3:186593/1‑141 (MQ=255)

                                                                                                         tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                     >  2:889776/1‑141 (MQ=255)

                                                                                                         tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                     >  2:897149/1‑141 (MQ=255)

                                                                                                          gAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGGTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGca                                    >  4:1293/1‑141 (MQ=255)

                                                                                                           aaaTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaa                                   >  1:649713/1‑141 (MQ=255)

                                                                                                           aaaTTTATCATTGCAATATTATGAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTGCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaa                                   <  3:379871/141‑1 (MQ=255)

                                                                                                             aTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaaca                                 <  2:236143/141‑1 (MQ=255)

                                                                                                                tATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAata                              >  2:57710/1‑141 (MQ=255)

                                                                                                                tATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCCTAAAAACGGAGTCTACTTATGCCCAAGTGCCAAAAGGCACGCAACAata                              >  2:893334/1‑141 (MQ=255)

                                                                                                                 aTCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAatat                             >  1:716976/1‑141 (MQ=255)

                                                                                                                  tCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATg                            >  1:52775/1‑141 (MQ=255)

                                                                                                                         aaTATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAatat                     <  4:105851/141‑1 (MQ=255)

                                                                                                                         aaTATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAatat                     <  4:427535/141‑1 (MQ=255)

                                                                                                                           tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCGAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                   <  1:997527/141‑1 (MQ=255)

                                                                                                                           tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                   >  2:687107/1‑141 (MQ=255)

                                                                                                                           tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                   <  2:85226/141‑1 (MQ=255)

                                                                                                                                ttaGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCt              <  2:489147/141‑1 (MQ=255)

                                                                                                                                ttaGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCt              <  2:130656/141‑1 (MQ=255)

                                                                                                                                  aGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCtct            >  1:703180/1‑139 (MQ=255)

                                                                                                                                    gATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTtata          <  1:383730/141‑1 (MQ=255)

                                                                                                                                           attatCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAag   <  2:896466/141‑1 (MQ=255)

                                                                                                                                            ttatCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAaga  >  3:190007/1‑141 (MQ=255)

ATGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAAGA  >  USA300TCH1516_ALE/1039277‑1039557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`