BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,039,416	A→C	5.3%	I12I (ATA→ATC)	USA300TCH1516_00965 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,039,416	0	A	C	5.3%	358.8 / 8.4	113	I12I (ATA→ATC)	USA300TCH1516_00965	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (46/61); new base C (4/2); total (50/63)
Fisher's exact test for biased strand distribution p-value = 4.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 120 total aligned reads displayed.

AATGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAAG  >  USA300TCH1516_ALE/1039276‑1039556

aaTGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAata                                                                                                                                              <  1:279684/141‑1 (MQ=255)

 atgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAatat                                                                                                                                             <  1:612692/141‑1 (MQ=255)

  tgatgTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGCTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGCTTTAACTATGAGATTTATCATTGCAATATTATTAGGATTAAtatt                                                                                                                                            >  1:584285/1‑141 (MQ=255)

      gTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATcc                                                                                                                                        >  2:183343/1‑141 (MQ=255)

         aTTTGCAACGATTCTTGTCGTTCACATATTATTGCAGATTTGCAGCCTATACTATCATGGCAGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCAtt                                                                                                                                     >  2:432441/1‑141 (MQ=255)

          tttGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTa                                                                                                                                    <  3:208796/141‑1 (MQ=255)

             gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                 <  3:399444/141‑1 (MQ=255)

             gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                 <  2:570505/141‑1 (MQ=255)

             gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                 <  4:284809/141‑1 (MQ=255)

             gCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTa                                                                                                                                 <  2:707719/141‑1 (MQ=255)

                aCGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTg                                                                                                                              <  1:256569/141‑1 (MQ=255)

                aCGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTg                                                                                                                              >  3:320186/1‑141 (MQ=255)

                   aTTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCtt                                                                                                                           <  1:639685/141‑1 (MQ=255)

                    ttCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGCTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCttt                                                                                                                          <  1:103628/141‑1 (MQ=255)

                     tCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCtttt                                                                                                                         <  2:590551/141‑1 (MQ=255)

                        tGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAGCGGTGAAATGTGTCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACa                                                                                                                      >  3:154385/1‑141 (MQ=255)

                        tGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACa                                                                                                                      >  4:188041/1‑141 (MQ=255)

                         gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCa                                                                                                                                       >  1:400903/1‑123 (MQ=255)

                         gTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCa                                                                                                                                       <  2:400903/123‑1 (MQ=255)

                            gTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCa                                                                                                                  <  2:151539/141‑1 (MQ=255)

                                aaaTATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAca                                                                                                              <  1:1073468/141‑1 (MQ=255)

                                   tattatTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAcatca                                                                                                           <  1:630110/141‑1 (MQ=255)

                                       attGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCt                                                                                                       <  2:561602/141‑1 (MQ=255)

                                        ttGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCta                                                                                                      <  3:443679/141‑1 (MQ=255)

                                           cAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATAc                                                                                                   <  1:303419/141‑1 (MQ=255)

                                             gATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTCTACtt                                                                                                 >  2:420101/1‑141 (MQ=255)

                                              aTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTg                                                                                                <  1:595067/141‑1 (MQ=255)

                                              aTTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTAGCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTg                                                                                                <  4:424197/141‑1 (MQ=255)

                                               tttGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGa                                                                                               <  2:198638/141‑1 (MQ=255)

                                                 tGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACCATCATACATCATCCTATACTTGATc                                                                                             <  2:565167/141‑1 (MQ=255)

                                                 tGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATc                                                                                             <  1:18220/141‑1 (MQ=255)

                                                     gCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGttt                                                                                         >  2:346644/1‑141 (MQ=255)

                                                     gCCTATACTAGCATATGCGACGTTTTAATGATGTTATCATGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTTTCCTTGATCGttt                                                                                         <  4:258030/141‑1 (MQ=255)

                                                      ccTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGtttt                                                                                        <  1:876357/141‑1 (MQ=255)

                                                      ccTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGtttt                                                                                        <  3:337148/141‑1 (MQ=255)

                                                       cTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTa                                                                                       >  4:326832/1‑141 (MQ=255)

                                                         ataCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAAt                                                                                     >  1:465623/1‑141 (MQ=255)

                                                          taCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATc                                                                                    <  1:863350/141‑1 (MQ=255)

                                                           aCTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATcc                                                                                   <  2:202306/141‑1 (MQ=255)

                                                              aTCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCttt                                                                                >  1:79394/1‑141 (MQ=255)

                                                               tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                               >  1:202464/1‑141 (MQ=255)

                                                               tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                               >  1:271725/1‑141 (MQ=255)

                                                               tCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTc                                                                               >  4:380244/1‑141 (MQ=255)

                                                                 aTGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCtt                                                                             >  2:941669/1‑141 (MQ=255)

                                                                 aTGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCtt                                                                             <  1:502845/141‑1 (MQ=255)

                                                                    gCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAACTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaa                                                                          <  1:854621/141‑1 (MQ=255)

                                                                      cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATTCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                        <  1:562442/141‑1 (MQ=255)

                                                                      cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                        >  2:1017362/1‑141 (MQ=255)

                                                                      cGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATCAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTaaaaa                                                                        >  1:785696/1‑141 (MQ=255)

                                                                       gACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAAc                                                                       <  2:395554/141‑1 (MQ=255)

                                                                       gACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAAc                                                                       <  1:470197/141‑1 (MQ=255)

                                                                        aCGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACg                                                                      >  3:55614/1‑141 (MQ=255)

                                                                         cGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACgg                                                                     >  3:235503/1‑141 (MQ=255)

                                                                           ttttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAg                                                                   <  1:719049/141‑1 (MQ=255)

                                                                            tttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGt                                                                  <  2:7675/141‑1 (MQ=255)

                                                                             ttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGta                                                                 >  2:583650/1‑141 (MQ=255)

                                                                             ttAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACGTGATCGTTTTAATCCTTTCTTAAAAACGGAGta                                                                 <  1:562102/141‑1 (MQ=255)

                                                                              tAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtat                                                                >  2:147628/1‑141 (MQ=255)

                                                                               aaggAAGTTATCAGTAATTTTAAACTATTAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACAGCTTCCTATGCTTGAGCGTTTTAAACCTTTCTAAAAAACGGAGtata                                                               <  2:52248/138‑1 (MQ=255)

                                                                               aaTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                               >  1:351098/1‑141 (MQ=255)

                                                                               aaTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                               >  2:997527/1‑141 (MQ=255)

                                                                               aaTGAAGTTATCAAGGAGTTTTAAATATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATAAATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGtata                                                               <  2:130734/141‑1 (MQ=255)

                                                                                aTGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAg                                                              >  1:75232/1‑141 (MQ=255)

                                                                                 tGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                             <  1:608637/141‑1 (MQ=255)

                                                                                 tGAAGTTATCAAGGAGTTCTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTCTACATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGt                                                             <  1:214460/141‑1 (MQ=255)

                                                                                   aaGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTa                                                           <  1:727815/141‑1 (MQ=255)

                                                                                      ttATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGc                                                        <  2:860193/141‑1 (MQ=255)

                                                                                        aTCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCa                                                      >  2:885037/1‑141 (MQ=255)

                                                                                         tCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCaa                                                     >  4:119994/1‑141 (MQ=255)

                                                                                           aaGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAg                                                   <  2:912639/141‑1 (MQ=255)

                                                                                              gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                <  1:96402/141‑1 (MQ=255)

                                                                                              gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                >  3:215444/1‑141 (MQ=255)

                                                                                              gAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGc                                                >  2:865938/1‑141 (MQ=255)

                                                                                                      aCTATGAAATTTATCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGACAAAGAGTCAAAAGGt                                        >  2:585257/1‑141 (MQ=255)

                                                                                                         aTGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACg                                     <  3:441318/141‑1 (MQ=255)

                                                                                                          tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                    >  2:897149/1‑141 (MQ=255)

                                                                                                          tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                    >  2:889776/1‑141 (MQ=255)

                                                                                                          tGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGc                                    >  3:186593/1‑141 (MQ=255)

                                                                                                           gAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGGTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGca                                   >  4:1293/1‑141 (MQ=255)

                                                                                                            aaaTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaa                                  >  1:649713/1‑141 (MQ=255)

                                                                                                            aaaTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaa                                  >  1:844124/1‑141 (MQ=255)

                                                                                                            aaaTTTATCATTGCAATATTATGAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTGCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaa                                  <  3:379871/141‑1 (MQ=255)

                                                                                                              aTTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGcaaca                                <  2:236143/141‑1 (MQ=255)

                                                                                                                 tATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAata                             >  1:973939/1‑141 (MQ=255)

                                                                                                                 tATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCCTAAAAACGGAGTCTACTTATGCCCAAGTGCCAAAAGGCACGCAACAata                             >  2:893334/1‑141 (MQ=255)

                                                                                                                  aTCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAatat                            >  1:716976/1‑141 (MQ=255)

                                                                                                                   tCATTGCAATATTATTAGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATg                           >  3:203871/1‑141 (MQ=255)

                                                                                                                   tCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATg                           >  2:265847/1‑141 (MQ=255)

                                                                                                                   tCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATg                           >  1:52775/1‑141 (MQ=255)

                                                                                                                          aaTATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAatat                    <  4:427535/141‑1 (MQ=255)

                                                                                                                          aaTATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAatat                    <  4:105851/141‑1 (MQ=255)

                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCGAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  <  1:997527/141‑1 (MQ=255)

                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  >  2:687107/1‑141 (MQ=255)

                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  <  2:85226/141‑1 (MQ=255)

                                                                                                                            tattatTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTa                  <  2:452618/141‑1 (MQ=255)

                                                                                                                                 ttaGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCt             <  2:130656/141‑1 (MQ=255)

                                                                                                                                 ttaGGATTAATCGTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCt             <  2:489147/141‑1 (MQ=255)

                                                                                                                                   aGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCtct           >  1:703180/1‑139 (MQ=255)

                                                                                                                                     gATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTtata         >  1:443713/1‑141 (MQ=255)

                                                                                                                                            attatCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAag  <  2:896466/141‑1 (MQ=255)

AATGATGTTATTTGCAACGATTCTTGTCGTTCAAATATTATTGCAGATTTGCAGCCTATACTATCATGGCCGACGTTTTAATGAAGTTATCAAGGAGTTTTAACTATGAAATTTATCATTGCAATATTATTAGGATTAATATTATCCATTACTATTGCTTTTACAATCATACATCATCCTATACTTGATCGTTTTAATCCTTTCTTAAAAACGGAGTATAGTTATGCCAAAGTGCCAAAAGGTACGCAACAATATGTTAATATTACGGCTTATAGTGAAAG  >  USA300TCH1516_ALE/1039276‑1039556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`