Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 1,034,878 | T→A | 5.0% | V67V (GTA→GTT) | USA300TCH1516_00961 ← | Lipoate‑‑protein ligase 1 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 1,034,878 | 0 | T | A | 5.0% | 380.0 / 8.0 | 119 | V67V (GTA→GTT) | USA300TCH1516_00961 | Lipoate‑‑protein ligase 1 |
Reads supporting (aligned to +/- strand): ref base T (50/63); new base A (4/2); total (54/65) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 |
Only 100 of 128 total aligned reads displayed.
AAAGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATA > USA300TCH1516_ALE/1034741‑1035018 | aaaGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTtact < 2:154781/141‑1 (MQ=255) aGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTtactac < 3:120443/141‑1 (MQ=255) gATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACa > 1:240863/1‑141 (MQ=255) aTTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACAt < 3:161001/141‑1 (MQ=255) aTTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACAt < 4:186356/141‑1 (MQ=255) ttGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATc < 4:248812/141‑1 (MQ=255) tGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCg < 1:362952/141‑1 (MQ=255) tGTAATGCTTGAACAAGTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGATCTAACAAAATAGCTCCAATAGCCAGTATCGTGAAACCCAGAGCCACCACCAGAAATTCTTCTTACTACATCg < 3:293507/141‑1 (MQ=255) gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGa < 3:328239/141‑1 (MQ=255) gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGa < 3:357530/141‑1 (MQ=255) gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGa > 3:370458/1‑141 (MQ=255) gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCATCTAACTACATCGa > 1:469655/1‑141 (MQ=255) gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCATCTAACTACATCGa > 1:469514/1‑141 (MQ=255) cTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATTATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGAtgctca < 2:313424/141‑5 (MQ=255) ttGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTtgtg < 2:924159/141‑1 (MQ=255) ttGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTtgtg < 1:475364/141‑1 (MQ=255) gAACAATTGGTTCAGTGAATTTTTTTTTTTTTTTTAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAg > 2:512987/1‑141 (MQ=255) aCAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCa > 4:301007/1‑141 (MQ=255) tGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGat > 2:626837/1‑141 (MQ=255) ggTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAACTCTTCTTACTACATCGATGTTGTGAGCATCGata > 2:692980/1‑141 (MQ=255) ttCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGatata < 4:76114/141‑1 (MQ=255) aTTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGAt < 2:473540/141‑1 (MQ=255) tttttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGAtt > 4:125254/1‑141 (MQ=255) tttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTa > 1:464165/1‑141 (MQ=255) tGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACt < 1:999272/141‑1 (MQ=255) tGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACt < 4:320195/141‑1 (MQ=255) ggAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACtt > 1:529001/1‑141 (MQ=255) aaaTTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTcc > 2:515325/1‑141 (MQ=255) gAAACTGTTACCATCATCATCTGTTATACAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt < 3:20225/141‑1 (MQ=255) gAAACTGTTACCATCATCATCTGTTATAATACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt < 3:20223/141‑1 (MQ=255) gAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt < 1:504160/141‑1 (MQ=255) gAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt < 4:45042/141‑1 (MQ=255) tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTAtttt < 2:484132/141‑1 (MQ=255) tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTAtttt < 3:128717/141‑1 (MQ=255) ttACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGa > 1:123934/1‑141 (MQ=255) aCCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGAtt < 1:241773/141‑1 (MQ=255) aCCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGAtt > 2:967621/1‑141 (MQ=255) ccATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTc > 2:276325/1‑141 (MQ=255) catcatcgtCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTAATTCt > 1:857869/1‑141 (MQ=255) catcatcatCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCAACGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCt < 2:919746/141‑1 (MQ=255) tcTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCcaaca < 1:1064342/141‑1 (MQ=255) aaaaCTAAAGTTTACAGCTCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGa < 1:603952/141‑1 (MQ=255) aaaCTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGAt < 3:429/141‑1 (MQ=255) aaCTANAGTTTAACTTGCCAGTATCCTGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGTATCGATATATGTCTGATTTACTTCCTCTNTCGTATTTTGATTCTTTCCAACAATGATAGATg < 1:132987/141‑1 (MQ=255) aCTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATgg < 1:210672/141‑1 (MQ=255) cTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGt > 1:362981/1‑141 (MQ=255) tAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTc > 2:1065617/1‑141 (MQ=255) tAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTc < 2:104109/141‑1 (MQ=255) aaaGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCt > 2:330937/1‑141 (MQ=255) aaGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTa > 1:598450/1‑141 (MQ=255) gTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTGTCGTATTTTGATTCTTTCCAACAATGTTAGATGGTCTAtt > 1:288911/1‑141 (MQ=255) aaaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATg < 3:190337/141‑1 (MQ=255) aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt > 2:38223/1‑141 (MQ=255) aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt > 3:323754/1‑141 (MQ=255) aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt > 1:698520/1‑141 (MQ=255) aTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATTGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGta < 2:197666/141‑1 (MQ=255) aTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGta < 1:141189/141‑1 (MQ=255) gCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATGTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaa < 4:31810/141‑1 (MQ=255) ccAGTATCATGATAAGCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaat < 1:265197/141‑1 (MQ=255) ccAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaat < 1:265238/141‑1 (MQ=255) aTCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATaaaaa < 2:893459/141‑1 (MQ=255) tCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAg > 2:374566/1‑141 (MQ=255) cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt < 4:408751/141‑1 (MQ=255) cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt < 4:26581/141‑1 (MQ=255) gATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAAc > 2:340035/1‑141 (MQ=255) gATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAAc > 1:669101/1‑141 (MQ=255) aTAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACt < 1:337950/141‑1 (MQ=255) aTAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGATGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACt < 3:64665/141‑1 (MQ=255) aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt < 4:438448/141‑1 (MQ=255) aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt > 4:80803/1‑141 (MQ=255) aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt > 2:614821/1‑141 (MQ=255) aaaCAGCGCCACCAACAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt < 4:438446/141‑1 (MQ=255) aCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTttct > 2:853115/1‑141 (MQ=255) cAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTttctt < 3:290547/141‑1 (MQ=255) aGCGCCACCACCAGAAATTCTTCTTACTACATCGAGGTCGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGAATATGTATGTAAAATAAAAAGTAANGtccttc > 2:813005/1‑141 (MQ=255) gcgcCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTtcttct > 3:61616/1‑141 (MQ=255) cgcCACCACCAGACATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTctg > 2:98404/1‑141 (MQ=255) ccaccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTctgct < 1:255808/141‑1 (MQ=255) caccaccaGAAATGCTTCTAACTACATCGATGTTATGAACATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAGAAAGTAACTTTCTTctgctt > 4:455982/1‑140 (MQ=255) accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg < 3:231155/141‑1 (MQ=255) accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg < 1:486156/141‑1 (MQ=255) ccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGt > 3:447527/1‑141 (MQ=255) ccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGt > 1:231895/1‑141 (MQ=255) ccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTAATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTaaa < 2:786267/141‑1 (MQ=255) caGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAt > 3:87225/1‑141 (MQ=255) aGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtt > 1:913/1‑141 (MQ=255) gAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAttt < 1:729597/141‑1 (MQ=255) aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt < 2:596598/141‑1 (MQ=255) aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt > 2:284171/1‑141 (MQ=255) aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt > 4:133966/1‑141 (MQ=255) aaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAttttt < 2:122772/141‑1 (MQ=255) aaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTAGTAAAttttt < 3:202757/141‑1 (MQ=255) ttcttcTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTa > 1:834695/1‑141 (MQ=255) tcttctTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaa > 2:925266/1‑141 (MQ=255) cttcttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaaa > 4:221275/1‑141 (MQ=255) cttcttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaaa > 1:777575/1‑141 (MQ=255) cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca < 4:104032/75‑1 (MQ=255) cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca > 3:104032/1‑75 (MQ=255) ttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACat < 1:994309/141‑1 (MQ=255) tactacATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACata < 2:698520/141‑1 (MQ=255) | AAAGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATA > USA300TCH1516_ALE/1034741‑1035018 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |