Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 1,034,878 T→A 5.0% V67V (GTA→GTT USA300TCH1516_00961 ← Lipoate‑‑protein ligase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE1,034,8780TA 5.0% 380.0 / 8.0 119V67V (GTA→GTTUSA300TCH1516_00961Lipoate‑‑protein ligase 1
Reads supporting (aligned to +/- strand):  ref base T (50/63);  new base A (4/2);  total (54/65)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 128 total aligned reads displayed.

AAAGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATA  >  USA300TCH1516_ALE/1034741‑1035018
                                                                                                                                         |                                                                                                                                            
aaaGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTtact                                                                                                                                           <  2:154781/141‑1 (MQ=255)
  aGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTtactac                                                                                                                                         <  3:120443/141‑1 (MQ=255)
   gATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACa                                                                                                                                        >  1:240863/1‑141 (MQ=255)
    aTTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACAt                                                                                                                                       <  3:161001/141‑1 (MQ=255)
    aTTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACAt                                                                                                                                       <  4:186356/141‑1 (MQ=255)
     ttGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATc                                                                                                                                      <  4:248812/141‑1 (MQ=255)
      tGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCg                                                                                                                                     <  1:362952/141‑1 (MQ=255)
      tGTAATGCTTGAACAAGTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGATCTAACAAAATAGCTCCAATAGCCAGTATCGTGAAACCCAGAGCCACCACCAGAAATTCTTCTTACTACATCg                                                                                                                                     <  3:293507/141‑1 (MQ=255)
       gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGa                                                                                                                                    <  3:328239/141‑1 (MQ=255)
       gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGa                                                                                                                                    <  3:357530/141‑1 (MQ=255)
       gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGa                                                                                                                                    >  3:370458/1‑141 (MQ=255)
       gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCATCTAACTACATCGa                                                                                                                                    >  1:469655/1‑141 (MQ=255)
       gTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCATCTAACTACATCGa                                                                                                                                    >  1:469514/1‑141 (MQ=255)
             cTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATTATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGAtgctca                                                                                                                              <  2:313424/141‑5 (MQ=255)
              ttGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTtgtg                                                                                                                             <  2:924159/141‑1 (MQ=255)
              ttGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTtgtg                                                                                                                             <  1:475364/141‑1 (MQ=255)
                gAACAATTGGTTCAGTGAATTTTTTTTTTTTTTTTAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAg                                                                                                                           >  2:512987/1‑141 (MQ=255)
                  aCAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCa                                                                                                                         >  4:301007/1‑141 (MQ=255)
                       tGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGat                                                                                                                    >  2:626837/1‑141 (MQ=255)
                        ggTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAACTCTTCTTACTACATCGATGTTGTGAGCATCGata                                                                                                                   >  2:692980/1‑141 (MQ=255)
                          ttCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGatata                                                                                                                 <  4:76114/141‑1 (MQ=255)
                                  aTTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGAt                                                                                                         <  2:473540/141‑1 (MQ=255)
                                   tttttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGAtt                                                                                                        >  4:125254/1‑141 (MQ=255)
                                     tttGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTa                                                                                                      >  1:464165/1‑141 (MQ=255)
                                       tGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACt                                                                                                    <  1:999272/141‑1 (MQ=255)
                                       tGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACt                                                                                                    <  4:320195/141‑1 (MQ=255)
                                        ggAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACtt                                                                                                   >  1:529001/1‑141 (MQ=255)
                                          aaaTTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTcc                                                                                                 >  2:515325/1‑141 (MQ=255)
                                                  gAAACTGTTACCATCATCATCTGTTATACAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt                                                                                         <  3:20225/141‑1 (MQ=255)
                                                  gAAACTGTTACCATCATCATCTGTTATAATACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt                                                                                         <  3:20223/141‑1 (MQ=255)
                                                  gAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt                                                                                         <  1:504160/141‑1 (MQ=255)
                                                  gAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGt                                                                                         <  4:45042/141‑1 (MQ=255)
                                                       tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTAtttt                                                                                    <  2:484132/141‑1 (MQ=255)
                                                       tGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTAACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTAtttt                                                                                    <  3:128717/141‑1 (MQ=255)
                                                         ttACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGa                                                                                  >  1:123934/1‑141 (MQ=255)
                                                           aCCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGAtt                                                                                <  1:241773/141‑1 (MQ=255)
                                                           aCCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGAtt                                                                                >  2:967621/1‑141 (MQ=255)
                                                            ccATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTc                                                                               >  2:276325/1‑141 (MQ=255)
                                                             catcatcgtCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTAATTCt                                                                              >  1:857869/1‑141 (MQ=255)
                                                             catcatcatCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCAACGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCt                                                                              <  2:919746/141‑1 (MQ=255)
                                                                     tcTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCcaaca                                                                      <  1:1064342/141‑1 (MQ=255)
                                                                             aaaaCTAAAGTTTACAGCTCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGa                                                              <  1:603952/141‑1 (MQ=255)
                                                                              aaaCTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGAt                                                             <  3:429/141‑1 (MQ=255)
                                                                               aaCTANAGTTTAACTTGCCAGTATCCTGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGTATCGATATATGTCTGATTTACTTCCTCTNTCGTATTTTGATTCTTTCCAACAATGATAGATg                                                            <  1:132987/141‑1 (MQ=255)
                                                                                aCTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATgg                                                           <  1:210672/141‑1 (MQ=255)
                                                                                 cTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGt                                                          >  1:362981/1‑141 (MQ=255)
                                                                                  tAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTc                                                         >  2:1065617/1‑141 (MQ=255)
                                                                                  tAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTc                                                         <  2:104109/141‑1 (MQ=255)
                                                                                   aaaGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCt                                                        >  2:330937/1‑141 (MQ=255)
                                                                                    aaGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTa                                                       >  1:598450/1‑141 (MQ=255)
                                                                                      gTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTGTCGTATTTTGATTCTTTCCAACAATGTTAGATGGTCTAtt                                                     >  1:288911/1‑141 (MQ=255)
                                                                                          aaaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATg                                                 <  3:190337/141‑1 (MQ=255)
                                                                                           aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt                                                >  2:38223/1‑141 (MQ=255)
                                                                                           aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt                                                >  3:323754/1‑141 (MQ=255)
                                                                                           aaTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGt                                                >  1:698520/1‑141 (MQ=255)
                                                                                            aTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATTGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGta                                               <  2:197666/141‑1 (MQ=255)
                                                                                            aTTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGta                                               <  1:141189/141‑1 (MQ=255)
                                                                                               gCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATGTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaa                                            <  4:31810/141‑1 (MQ=255)
                                                                                                ccAGTATCATGATAAGCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaat                                           <  1:265197/141‑1 (MQ=255)
                                                                                                ccAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGtaaaat                                           <  1:265238/141‑1 (MQ=255)
                                                                                                     aTCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATaaaaa                                      <  2:893459/141‑1 (MQ=255)
                                                                                                      tCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAg                                     >  2:374566/1‑141 (MQ=255)
                                                                                                       cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt                                    <  4:408751/141‑1 (MQ=255)
                                                                                                       cATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGt                                    <  4:26581/141‑1 (MQ=255)
                                                                                                          gATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAAc                                 >  2:340035/1‑141 (MQ=255)
                                                                                                          gATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAAc                                 >  1:669101/1‑141 (MQ=255)
                                                                                                           aTAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACt                                <  1:337950/141‑1 (MQ=255)
                                                                                                           aTAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGATGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACt                                <  3:64665/141‑1 (MQ=255)
                                                                                                             aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                              <  4:438448/141‑1 (MQ=255)
                                                                                                             aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                              >  4:80803/1‑141 (MQ=255)
                                                                                                             aaaCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                              >  2:614821/1‑141 (MQ=255)
                                                                                                             aaaCAGCGCCACCAACAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACttt                              <  4:438446/141‑1 (MQ=255)
                                                                                                               aCAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTttct                            >  2:853115/1‑141 (MQ=255)
                                                                                                                cAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTttctt                           <  3:290547/141‑1 (MQ=255)
                                                                                                                 aGCGCCACCACCAGAAATTCTTCTTACTACATCGAGGTCGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGAATATGTATGTAAAATAAAAAGTAANGtccttc                          >  2:813005/1‑141 (MQ=255)
                                                                                                                  gcgcCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTtcttct                         >  3:61616/1‑141 (MQ=255)
                                                                                                                   cgcCACCACCAGACATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTctg                        >  2:98404/1‑141 (MQ=255)
                                                                                                                     ccaccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTctgct                      <  1:255808/141‑1 (MQ=255)
                                                                                                                      caccaccaGAAATGCTTCTAACTACATCGATGTTATGAACATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAGAAAGTAACTTTCTTctgctt                     >  4:455982/1‑140 (MQ=255)
                                                                                                                       accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg                    <  3:231155/141‑1 (MQ=255)
                                                                                                                       accaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTgg                    <  1:486156/141‑1 (MQ=255)
                                                                                                                        ccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGt                   >  3:447527/1‑141 (MQ=255)
                                                                                                                        ccaccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGt                   >  1:231895/1‑141 (MQ=255)
                                                                                                                           ccaGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTAATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTaaa                <  2:786267/141‑1 (MQ=255)
                                                                                                                            caGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAt               >  3:87225/1‑141 (MQ=255)
                                                                                                                             aGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtt              >  1:913/1‑141 (MQ=255)
                                                                                                                              gAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAttt             <  1:729597/141‑1 (MQ=255)
                                                                                                                               aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt            <  2:596598/141‑1 (MQ=255)
                                                                                                                               aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt            >  2:284171/1‑141 (MQ=255)
                                                                                                                               aaaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAtttt            >  4:133966/1‑141 (MQ=255)
                                                                                                                                aaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAAttttt           <  2:122772/141‑1 (MQ=255)
                                                                                                                                aaTTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTAGTAAAttttt           <  3:202757/141‑1 (MQ=255)
                                                                                                                                  ttcttcTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTa         >  1:834695/1‑141 (MQ=255)
                                                                                                                                   tcttctTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaa        >  2:925266/1‑141 (MQ=255)
                                                                                                                                    cttcttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaaa       >  4:221275/1‑141 (MQ=255)
                                                                                                                                    cttcttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTaaa       >  1:777575/1‑141 (MQ=255)
                                                                                                                                    cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca                                                                         <  4:104032/75‑1 (MQ=255)
                                                                                                                                    cttctaACTACATCGATGTTATGAGCATCTATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCca                                                                         >  3:104032/1‑75 (MQ=255)
                                                                                                                                        ttACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACat   <  1:994309/141‑1 (MQ=255)
                                                                                                                                         tactacATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACata  <  2:698520/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
AAAGATTGTAATGCTTGAACAATTGGTTCAGTGAATTTTTGGAAATTGTGGAAACTGTTACCATCATCATCTGTTATAAAACTAAAGTTTAAATTGCCAGTATCATGATAAACAGCGCCACCACCAGAAATTCTTCTTACTACATCGATGTTGTGAGCATCGATATATGTCTGATTTACTTCCTCTATCGTATTTTGATTCTTTCCAACAATGATAGATGGTCTATTTATGTAAAATAAAAAGTAACTTTCTTCTGCTGGTAAATTTTTTAAAACATA  >  USA300TCH1516_ALE/1034741‑1035018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: