Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 985,066 T→A 5.6% intergenic (‑131/‑164) USA300HOU_RS04870 ← / → fabH hypothetical protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE985,0660TA 5.6% 403.7 / 12.8 125intergenic (‑131/‑164)USA300HOU_RS04870/fabHhypothetical protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase 3
Reads supporting (aligned to +/- strand):  ref base T (61/57);  new base A (4/3);  total (65/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-01

Only 100 of 126 total aligned reads displayed.

GGTGTTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAA  >  USA300TCH1516_ALE/984926‑985205
                                                                                                                                            |                                                                                                                                           
ggTGTTTCCTGTCCTTCTCCCCCCCCTTTAATTATATATATTTTCTCTTCACTGAAAGTTCGACCTTTGTCTAGTAAACTCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCt                                                                                                                                             <  3:224211/141‑1 (MQ=255)
   gtTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTaaa                                                                                                                                          >  2:1074593/1‑141 (MQ=255)
    tttCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAAt                                                                                                                                         >  4:308563/1‑141 (MQ=255)
     ttCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAAtt                                                                                                                                        <  4:244774/141‑1 (MQ=255)
       cATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTaa                                                                                                                                      >  1:69784/1‑141 (MQ=255)
       cATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTaa                                                                                                                                      >  4:123822/1‑141 (MQ=255)
         tGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAgc                                                                                                                                    <  2:96308/141‑1 (MQ=255)
          gTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAgcg                                                                                                                                   <  4:122770/141‑1 (MQ=255)
            cATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCa                                                                                                                                 <  1:185184/141‑1 (MQ=255)
            cATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCa                                                                                                                                 >  2:684279/1‑141 (MQ=255)
             aTTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCAt                                                                                                                                <  1:570883/141‑1 (MQ=255)
                   ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTaaagc                                                                                                                          >  1:391161/1‑139 (MQ=255)
                   ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAc                                                                                                                          >  1:337538/1‑141 (MQ=255)
                   ccTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAc                                                                                                                          >  1:388776/1‑141 (MQ=255)
                    cTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAAcc                                                                                                                         <  2:719926/141‑1 (MQ=255)
                         cTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAAttt                                                                                                                    >  1:966606/1‑141 (MQ=255)
                             aaTTATATATATTTTATATTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCGTATATTTTGACCATATTATACAGTTTATATAAATGATTTTATCTGAATGGCTAGTCTAAATTAAGCGCATTAAAACCAATTTCATa                                                                                                                >  4:342569/1‑141 (MQ=255)
                               ttATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACt                                                                                                              <  1:522745/141‑1 (MQ=255)
                                tatatataTTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTGTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTg                                                                                                             <  3:375187/141‑1 (MQ=255)
                                 atatatatTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGa                                                                                                            >  1:133240/1‑141 (MQ=255)
                                  tttatatTTTATTTGAAGTGAAAGTTTGAAATTTGTATAGTAACATCTCATATATTTTGACCTTATTATTCAGTTTTAATAAATGATTTTATCTTAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGat                                                                                                           <  2:724159/139‑2 (MQ=255)
                                  tatatatTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGaa                                                                                                           <  1:1012059/141‑1 (MQ=255)
                                      tattttatttTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAGATTAAGCGCATTAAAGCCAATTTCATACTGAAAttt                                                                                                       <  2:291195/141‑1 (MQ=255)
                                         tttattttaAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGAc                                                                                                    <  1:36176/141‑1 (MQ=255)
                                          ttattttaAGTGAAAGTTAGAAATTTGTATAGTAACATCTAATATATTTTGACCATATTATAGAGTTTAACTAAATGATTTTATCTGAATGGCTATTATAAATTGCGCGCATTAAAACCACTTTCATACTGAAATTTGACg                                                                                                   <  2:500345/141‑1 (MQ=255)
                                          ttattttaAGTGAAAGTTAGAAAATTGTATAGCAACATCTTATATATTTAGATGATACTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACg                                                                                                   <  1:1024935/141‑1 (MQ=255)
                                                  aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                           >  1:888025/1‑141 (MQ=255)
                                                  aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                           >  2:734452/1‑141 (MQ=255)
                                                  aGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATaaa                                                                                           >  2:236051/1‑141 (MQ=255)
                                                       aaGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCAtt                                                                                      >  1:99610/1‑141 (MQ=255)
                                                       aaGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCAtt                                                                                      <  3:323270/141‑1 (MQ=255)
                                                        aGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTa                                                                                     >  4:134979/1‑141 (MQ=255)
                                                         gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                    >  4:261413/1‑141 (MQ=255)
                                                         gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                    >  1:174001/1‑141 (MQ=255)
                                                         gTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaa                                                                                    <  2:829989/141‑1 (MQ=255)
                                                           tAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaaaa                                                                                  <  4:362996/141‑1 (MQ=255)
                                                           tAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTaaaa                                                                                  <  4:184494/141‑1 (MQ=255)
                                                            aGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAt                                                                                 <  3:401112/141‑1 (MQ=255)
                                                            aGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAt                                                                                 >  2:317137/1‑141 (MQ=255)
                                                             gAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtt                                                                                <  1:127921/141‑1 (MQ=255)
                                                             gAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtt                                                                                >  4:283926/1‑141 (MQ=255)
                                                               aaTTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAAtttt                                                                              >  4:259204/1‑141 (MQ=255)
                                                                aTTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTtta                                                                             >  2:115767/1‑141 (MQ=255)
                                                                aTTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTtta                                                                             >  1:1045392/1‑141 (MQ=255)
                                                                 tttGTNTAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCANACTNAAATTTGACGATAATAAAGCATTAAAATTttat                                                                            <  1:384154/141‑1 (MQ=255)
                                                                 tttGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttat                                                                            <  1:1074593/141‑1 (MQ=255)
                                                                   tGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTttatta                                                                          >  2:172757/1‑141 (MQ=255)
                                                                   tGTATAGTAACATCTCATATATTTTGAACATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATACAGCATTCCCATTttatta                                                                          >  2:643959/1‑141 (MQ=255)
                                                                    gTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTaa                                                                         >  2:907418/1‑141 (MQ=255)
                                                                        aGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGTATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAg                                                                     <  2:286802/141‑1 (MQ=255)
                                                                        aGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAg                                                                     <  2:144709/141‑1 (MQ=255)
                                                                         gTAACATCTCATATATTTTGACCATATTATACAGTTTAACTAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGt                                                                    <  1:799932/141‑1 (MQ=255)
                                                                         gTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGt                                                                    >  2:621054/1‑141 (MQ=255)
                                                                            aCATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCaa                                                                 <  1:172757/141‑1 (MQ=255)
                                                                               tctcATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAatat                                                              >  1:406460/1‑141 (MQ=255)
                                                                                       atTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACct                                                      <  1:184588/141‑1 (MQ=255)
                                                                                        ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACctt                                                     <  1:1030670/141‑2 (MQ=255)
                                                                                        ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACctc                                                     >  2:1018622/1‑141 (MQ=255)
                                                                                        ttttGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCGATTCTAAATTAAGCGCACTAAAACCAATTTCATACTGAAATTTGACGATATTAACGCATTAAATTTTTATTAACTAGTTAATATTcctccctc                                                     <  4:400825/141‑1 (MQ=255)
                                                                                          ttGATCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTg                                                   <  2:866851/141‑1 (MQ=255)
                                                                                               cATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTg                                              >  3:54774/1‑141 (MQ=255)
                                                                                                atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                             <  2:607920/141‑1 (MQ=255)
                                                                                                atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                             <  1:995026/141‑1 (MQ=255)
                                                                                                atatTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGa                                             <  1:968526/141‑1 (MQ=255)
                                                                                                   ttatACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGtt                                          >  1:286453/1‑141 (MQ=255)
                                                                                                    tataCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGttt                                         <  1:960779/141‑1 (MQ=255)
                                                                                                     ataCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTa                                        >  3:416731/1‑141 (MQ=255)
                                                                                                       aCAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTaaa                                      >  4:122649/1‑141 (MQ=255)
                                                                                                           tttAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGt                                  <  4:351131/141‑1 (MQ=255)
                                                                                                            ttAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTa                                 <  4:165497/141‑1 (MQ=255)
                                                                                                               aataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATc                              <  1:940474/141‑1 (MQ=255)
                                                                                                               aataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATc                              >  2:448835/1‑141 (MQ=255)
                                                                                                               aataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATc                              >  3:92615/1‑141 (MQ=255)
                                                                                                                ataaatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCt                             >  1:490071/1‑141 (MQ=255)
                                                                                                                   aatGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATg                          <  3:226512/141‑1 (MQ=255)
                                                                                                                      gATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTTAGTTTAAAAAGTAATCTATGTTa                       >  2:477076/1‑141 (MQ=255)
                                                                                                                      gATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTa                       <  4:180903/141‑1 (MQ=255)
                                                                                                                       aTTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTaa                      <  2:920297/141‑1 (MQ=255)
                                                                                                                         tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTCTGTTAAAt                    >  1:126092/1‑141 (MQ=255)
                                                                                                                         tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                    >  2:295427/1‑141 (MQ=255)
                                                                                                                         tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAt                    >  1:456779/1‑141 (MQ=255)
                                                                                                                         tttATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGATTAAAAAGTAATCTATGTTAAAt                    <  2:451394/141‑1 (MQ=255)
                                                                                                                          ttATCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAAtt                   <  1:907418/141‑1 (MQ=255)
                                                                                                                            aTCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTaa                 >  2:242949/1‑141 (MQ=255)
                                                                                                                             tCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAAt                >  1:357542/1‑141 (MQ=255)
                                                                                                                             tCTGAATGGCTATTCAAATTTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAAt                >  1:576105/1‑141 (MQ=255)
                                                                                                                              cTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATa               >  3:304565/1‑141 (MQ=255)
                                                                                                                               tGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAc              >  1:719684/1‑141 (MQ=255)
                                                                                                                                gAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAcc             >  4:349793/1‑141 (MQ=255)
                                                                                                                                gAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATAcc             <  1:794212/141‑1 (MQ=255)
                                                                                                                                    ggCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGt         >  2:1013773/1‑141 (MQ=255)
                                                                                                                                     gCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTa        <  3:431910/141‑1 (MQ=255)
                                                                                                                                     gCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTa        <  3:369280/141‑1 (MQ=255)
                                                                                                                                      cTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAt       <  1:540064/141‑1 (MQ=255)
                                                                                                                                       tATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAtt      <  4:418810/141‑1 (MQ=255)
                                                                                                                                       tATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAtt      >  4:444431/1‑141 (MQ=255)
                                                                                                                                       tATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTAtt      >  1:634510/1‑141 (MQ=255)
                                                                                                                                         ttCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTaa    >  1:377990/1‑141 (MQ=255)
                                                                                                                                          tCTAAATTAAACGCATTAAAACCAACATCGTACTGAAATTTGACTATAATAATGCATTAGAATTTTATTAACTAGTCAATATTCGTATCTCTGACTTGAGATGAAAAAGTAAACTATGTTAAGTTGACCCCTGGTCTTacg   <  2:381992/141‑3 (MQ=255)
                                                                                                                                           cTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTaaaa  <  1:743552/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GGTGTTTCATGTCATTCTACCTCCACTTTAATTATATATATTTTATTTTAAGTGAAAGTTAGAAATTTGTATAGTAACATCTCATATATTTTGACCATATTATACAGTTTAAATAAATGATTTTATCTGAATGGCTATTCTAAATTAAGCGCATTAAAACCAATTTCATACTGAAATTTGACGATAATAAAGCATTAAAATTTTATTAACTAGTCAATATTCCTACCTCTGACTTGAGTTTAAAAAGTAATCTATGTTAAATTAATACCTGGTATTAAAA  >  USA300TCH1516_ALE/984926‑985205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: