BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	950,098	T→A	6.1%	intergenic (+92/‑270)	yugI_2 → / → USA300HOU_RS04740	General stress protein 13/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	950,098	0	T	A	6.1%	429.2 / 13.1	132	intergenic (+92/‑270)	yugI_2/USA300HOU_RS04740	General stress protein 13/putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (67/56); new base A (3/5); total (71/61)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 143 total aligned reads displayed.

CTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGTTTTTT  >  USA300TCH1516_ALE/949958‑950238

 ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt                                                                                                                                               >  1:1012550/1‑141 (MQ=255)

 ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt                                                                                                                                               >  3:20115/1‑141 (MQ=255)

     cAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGAt                                                                                                                                           <  4:349218/141‑1 (MQ=255)

        tCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACt                                                                                                                                        <  2:290182/141‑1 (MQ=255)

          tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTaa                                                                                                                                      <  1:418807/141‑1 (MQ=255)

            gATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtc                                                                                                                                    >  3:88767/1‑141 (MQ=255)

             aTAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtct                                                                                                                                   <  1:327005/141‑1 (MQ=255)

              tAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtctc                                                                                                                                  >  2:219861/1‑141 (MQ=255)

               aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                                 <  1:122163/141‑1 (MQ=255)

                aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCtt                                                                                                                                <  2:433058/141‑1 (MQ=255)

                aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTGCTTATACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCtt                                                                                                                                <  2:533323/141‑1 (MQ=38)

                 aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                               >  4:139018/1‑141 (MQ=255)

                 aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                               <  2:34584/141‑1 (MQ=255)

                 aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt                                                                                                                               >  3:86460/1‑141 (MQ=255)

                    attCAAAGCGAGCAATGCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                          <  2:124506/139‑1 (MQ=255)

                    aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g                                                                                                                          <  1:712250/141‑1 (MQ=255)

                        aaaGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGACTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTa                                                                                                                      >  2:443723/1‑141 (MQ=255)

                          aGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAACATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACg                                                                                                                    >  3:376938/1‑141 (MQ=255)

                             gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATa                                                                                                                 >  1:287068/1‑141 (MQ=255)

                               gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                               >  3:404276/1‑141 (MQ=255)

                               gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc                                                                                                               >  1:303422/1‑141 (MQ=255)

                                 aaTTCGAAACGACTAAAGGACCAGATAAATCGTACCGAAAAACATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                             <  1:597274/141‑1 (MQ=255)

                                 aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                             <  1:91140/141‑1 (MQ=255)

                                 aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt                                                                                                             >  2:430869/1‑141 (MQ=255)

                                   ttCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTaa                                                                                                           >  2:238286/1‑141 (MQ=255)

                                    tCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAat                                                                                                          >  2:621314/1‑141 (MQ=255)

                                     cGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAata                                                                                                         >  2:878168/1‑141 (MQ=255)

                                       aaaCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtg                                                                                                       <  1:104037/141‑1 (MQ=255)

                                              tAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATgattgatt                                                                                                <  1:643000/141‑1 (MQ=255)

                                               aaaGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTc                                                                                               <  1:808900/141‑1 (MQ=255)

                                                  ggAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTAt                                                                                            >  1:712956/1‑141 (MQ=255)

                                                     aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGAATGATTCTATTTa                                                                                         >  4:367281/1‑141 (MQ=255)

                                                     aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑G‑ATCTACGATAACGTAATATGATTGATTCTATTTa                                                                                         >  2:602685/1‑141 (MQ=255)

                                                      cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGGAATGAAAGTTTCTTAGGCTATAAAAGAGGTTAGTATTTATTAAATGTTAGTATATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                        >  3:42567/1‑141 (MQ=255)

                                                      cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                        <  3:249557/141‑1 (MQ=255)

                                                      cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac                                                                                        <  1:206173/141‑1 (MQ=255)

                                                         aTAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACg                                                                                     >  1:10211/1‑141 (MQ=255)

                                                                 tACCGAAAATCATACATATTGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg                                                                             <  3:136246/141‑1 (MQ=255)

                                                                 tACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg                                                                             >  2:1030898/1‑141 (MQ=255)

                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTTTTTTAACGTAATATGATTGATTCTATTTACACTTACAAATgg                                                                            >  4:417071/1‑141 (MQ=255)

                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAACTgg                                                                            >  2:807678/1‑141 (MQ=255)

                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg                                                                            >  4:173339/1‑141 (MQ=255)

                                                                  aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg                                                                            >  1:994858/1‑141 (MQ=255)

                                                                   ccGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGt                                                                           <  2:333389/141‑1 (MQ=255)

                                                                    cGAAAATCATACAAAGGGTGTGAAATGAAAGTTTCTTAGACTATAAAAGGGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGtt                                                                          <  2:935344/141‑1 (MQ=255)

                                                                    cGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGtt                                                                          <  3:144825/141‑1 (MQ=255)

                                                                       aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa                                                                       >  2:843095/1‑141 (MQ=255)

                                                                       aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTGT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa                                                                       >  3:18537/1‑141 (MQ=255)

                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                                 <  1:845946/79‑1 (MQ=255)

                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct                                                                                                                                 >  2:845946/1‑79 (MQ=255)

                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                                 >  2:1054256/1‑141 (MQ=255)

                                                                             tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc                                                                 >  2:774569/1‑141 (MQ=255)

                                                                              aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACa                                                                >  2:63508/1‑141 (MQ=255)

                                                                              aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATCTACACGTACAAATGGTTTAAGGTGACa                                                                >  2:1011674/1‑141 (MQ=255)

                                                                               cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                               <  2:1041038/141‑1 (MQ=255)

                                                                               cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat                                                               >  2:560165/1‑141 (MQ=255)

                                                                                  aGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATc                                                            <  2:261969/141‑1 (MQ=255)

                                                                                   gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                           <  1:519489/141‑1 (MQ=255)

                                                                                   gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                           >  1:389866/1‑141 (MQ=255)

                                                                                   gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc                                                           <  2:484411/141‑1 (MQ=255)

                                                                                     gTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAt                                                         <  4:71101/141‑1 (MQ=255)

                                                                                      tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTGGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                        >  1:655431/1‑141 (MQ=255)

                                                                                      tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt                                                        >  1:571585/1‑141 (MQ=255)

                                                                                       cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa                                                       >  1:249847/1‑141 (MQ=255)

                                                                                         gaaatgaaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATc                                                     >  2:958084/1‑141 (MQ=255)

                                                                                               aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt                                               <  1:624837/141‑1 (MQ=255)

                                                                                               aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt                                               <  1:634127/141‑1 (MQ=255)

                                                                                                  gTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTGTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtaga                                            <  1:427531/141‑1 (MQ=255)

                                                                                                   tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat                                           >  2:951778/1‑141 (MQ=255)

                                                                                                    ttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagata                                          >  2:90030/1‑141 (MQ=255)

                                                                                                        tAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAAt                                      >  3:356217/1‑141 (MQ=255)

                                                                                                         aGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATc                                     <  1:678644/141‑1 (MQ=255)

                                                                                                          gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGGCATATCCATTATCTTTGTTAGATAGAATCg                                    <  1:432801/141‑1 (MQ=255)

                                                                                                          gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCg                                    <  4:189670/141‑1 (MQ=255)

                                                                                                            cTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATTCATTATCTTTGTTAGATAGAATCGtt                                  >  2:278320/1‑141 (MQ=255)

                                                                                                             acaaaaaGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCTTTTATAGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGt                                   >  1:81670/4‑141 (MQ=25)

                                                                                                              ataAAAGCGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTCAAACAGGTTTAACGTGATATCTCTATTCTCTTTGTTAGATAGAATCGTTGa                                <  4:42567/141‑1 (MQ=255)

                                                                                                                aaaaGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGAtt                              >  3:247641/1‑141 (MQ=255)

                                                                                                                 aaaGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGAttt                             <  3:334081/141‑1 (MQ=255)

                                                                                                                   agagATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGc                           <  2:217245/141‑1 (MQ=255)

                                                                                                                      gaTTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAat                        <  2:143668/141‑1 (MQ=255)

                                                                                                                        ttAGTATCTAATAAAATTTAATAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAatat                      >  2:490241/1‑141 (MQ=255)

                                                                                                                         tAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATAtt                     >  3:420955/1‑141 (MQ=255)

                                                                                                                          aGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTg                    >  4:76049/1‑141 (MQ=255)

                                                                                                                          aGTATCTAATAAAATTTAATAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTg                    >  1:289595/1‑141 (MQ=255)

                                                                                                                           gTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt                   >  1:192089/1‑141 (MQ=255)

                                                                                                                           gTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt                   <  4:297315/141‑1 (MQ=255)

                                                                                                                               cTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTAtgt               <  2:598500/141‑1 (MQ=255)

                                                                                                                                 aTTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTgg             <  1:33427/141‑1 (MQ=255)

                                                                                                                                  ttAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGa            <  2:684360/141‑1 (MQ=255)

                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTT‑T‑TTTTTTTTTTTTATAATATGATTGATTTTATTTATAAGTACAATTGGTTTAGGGTAACATATCTATTATTTTGGTTAGATAGAATCTTTGATTTTCATTATTGAATGTGTAtt          >  2:837673/1‑141 (MQ=255)

                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt          >  2:189212/1‑141 (MQ=255)

                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt          >  4:229379/1‑141 (MQ=255)

                                                                                                                                    aaaTTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGAtt          <  4:29342/141‑1 (MQ=255)

                                                                                                                                       ttttATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGt       >  1:661310/1‑141 (MQ=255)

                                                                                                                                         ttattaGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGttt     <  1:914583/141‑1 (MQ=255)

                                                                                                                                           attaGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGttttt   >  4:172518/1‑141 (MQ=255)

                                                                                                                                           attaGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGttttt   >  3:33354/1‑141 (MQ=255)

                                                                                                                                           attaGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGACAGAATCGTTGATTTGCAATATTGTATGTGGATTTgttgtt   >  1:15026/1‑141 (MQ=255)

                                                                                                                                            ttaGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGtttttt  <  4:197270/141‑1 (MQ=255)

CTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGATTTGTTTTTT  >  USA300TCH1516_ALE/949958‑950238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`