| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 950,088 | T→A | 5.4% | intergenic (+82/‑280) | yugI_2 → / → USA300HOU_RS04740 | General stress protein 13/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 950,088 | 0 | T | A | 5.4% | 482.7 / 12.7 | 148 | intergenic (+82/‑280) | yugI_2/USA300HOU_RS04740 | General stress protein 13/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (80/60); new base A (3/5); total (83/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
Only 100 of 149 total aligned reads displayed.
AAAGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGA > USA300TCH1516_ALE/949950‑950228 | aaaGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAATGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTa < 1:745329/141‑1 (MQ=255) aaGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTaa > 3:197270/1‑141 (MQ=255) aaGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTaa > 2:477280/1‑141 (MQ=255) aaGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTaa > 3:208770/1‑141 (MQ=255) aaGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTaa > 2:133633/1‑141 (MQ=255) aaGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTaa > 3:342021/1‑141 (MQ=255) aGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTaaa > 2:871420/1‑141 (MQ=255) aGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTaaa > 1:942689/1‑141 (MQ=255) gAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAt < 2:770514/141‑1 (MQ=255) gAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAt < 4:264666/141‑1 (MQ=255) ccGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttt > 2:520309/2‑141 (MQ=255) aCGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAGACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttt < 2:745321/141‑1 (MQ=255) aCGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttt > 3:384373/1‑141 (MQ=255) gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAAttatt > 3:157783/1‑138 (MQ=255) gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta > 2:91140/1‑141 (MQ=255) gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta > 2:21981/1‑141 (MQ=255) gCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTtta > 1:143668/1‑141 (MQ=255) ttACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatt > 1:1012550/1‑141 (MQ=255) tACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAGAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTttatta > 1:692197/1‑141 (MQ=255) cAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGAt < 4:349218/141‑1 (MQ=255) tCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACt < 2:290182/141‑1 (MQ=255) tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTaa < 1:439062/141‑1 (MQ=255) tGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTaa < 1:418807/141‑1 (MQ=255) tAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAAtctc > 2:219861/1‑141 (MQ=255) aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct < 2:390146/141‑1 (MQ=255) aaaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct < 1:122163/141‑1 (MQ=255) aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCtt < 2:433058/141‑1 (MQ=255) aaaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTGCTTATACTATAAAAGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCtt < 2:533323/141‑1 (MQ=38) aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt < 2:34584/141‑1 (MQ=255) aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt > 3:86460/1‑141 (MQ=255) aaCAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCttt > 4:139018/1‑141 (MQ=255) attCAAAGCGAGCAATGCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g < 2:124506/139‑1 (MQ=255) aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g > 1:656900/1‑141 (MQ=255) aGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑g < 1:712250/141‑1 (MQ=255) gTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑Gt > 2:594774/1‑141 (MQ=255) tCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTc < 1:33392/141‑1 (MQ=255) aaaGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGACTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTa > 2:443723/1‑141 (MQ=255) aGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAACATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACg > 3:376938/1‑141 (MQ=255) gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATa > 1:287068/1‑141 (MQ=255) gAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATa < 2:870245/141‑1 (MQ=255) gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc > 4:144825/1‑141 (MQ=255) gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc > 1:303422/1‑141 (MQ=255) gCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAAc > 3:404285/1‑141 (MQ=255) aaTTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGt < 1:91140/141‑1 (MQ=255) tCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAat > 2:621314/1‑141 (MQ=255) aaaCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtg < 1:104037/141‑1 (MQ=255) aCGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATAtgat > 3:285613/1‑141 (MQ=255) aCAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGAATGATTCTATTTa > 4:367281/1‑141 (MQ=255) cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac < 1:206173/141‑1 (MQ=255) cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac < 3:249557/141‑1 (MQ=255) cAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTac < 1:411100/141‑1 (MQ=255) aTAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACg > 1:10211/1‑141 (MQ=255) tACCGAAAATCATACATATTGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg < 3:136246/141‑1 (MQ=255) tACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATg > 2:1030898/1‑141 (MQ=255) aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTTTTTTAACGTAATATGATTGATTCTATTTACACTTACAAATgg > 4:417071/1‑141 (MQ=255) aCCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATgg > 1:994858/1‑141 (MQ=255) cGAAAATCATACAAAGGGTGTGAAATGAAAGTTTCTTAGACTATAAAAGGGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGtt < 2:935344/141‑1 (MQ=255) aaaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTa > 1:301794/1‑141 (MQ=255) aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa > 2:843095/1‑141 (MQ=255) aaaTCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTGT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTaa > 3:18537/1‑141 (MQ=255) tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATctct < 1:845946/79‑1 (MQ=255) tACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGAc > 2:774569/1‑141 (MQ=255) aCAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATCTACACGTACAAATGGTTTAAGGTGACa > 2:1011674/1‑141 (MQ=255) cAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACat < 2:1041038/141‑1 (MQ=255) aGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATc < 2:261969/141‑1 (MQ=255) gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc < 3:211416/141‑1 (MQ=255) gggTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATcc < 2:484411/141‑1 (MQ=255) gTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAt < 4:71101/141‑1 (MQ=255) tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTGGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt > 1:655431/1‑141 (MQ=255) tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt > 1:571585/1‑141 (MQ=255) tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt < 3:161475/141‑1 (MQ=255) tCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCAtt > 2:290685/1‑141 (MQ=255) cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa > 1:249847/1‑141 (MQ=255) cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa > 2:626708/1‑141 (MQ=255) cTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTa < 3:52377/141‑1 (MQ=255) gaaatgaaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATc > 2:958084/1‑141 (MQ=255) aaatgaaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCt > 2:824612/1‑141 (MQ=255) aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt < 1:634127/141‑1 (MQ=255) aaaGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGtt < 1:624837/141‑1 (MQ=255) tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat > 2:951778/1‑141 (MQ=255) tttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagat < 1:594878/141‑1 (MQ=255) ttCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTtagata > 2:90030/1‑141 (MQ=255) tAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAAt > 3:356217/1‑141 (MQ=255) gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGGCATATCCATTATCTTTGTTAGATAGAATCg < 1:432801/141‑1 (MQ=255) gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCg < 4:189670/141‑1 (MQ=255) gACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCg < 4:180139/141‑1 (MQ=255) cTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGtt < 2:272938/141‑1 (MQ=255) acaaaaaGAGATTAGTATCTAATAAAATTTAATAGATACTAATCTCTTTTATAGTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGt > 1:81670/4‑141 (MQ=25) ataAAAGCGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTCAAACAGGTTTAACGTGATATCTCTATTCTCTTTGTTAGATAGAATCGTTGa < 4:42567/141‑1 (MQ=255) aaaaGAGATTAGTAACTTTTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTCGTTAGATAGAATCGTTGAtt > 1:800992/1‑141 (MQ=255) agagATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGc < 2:217245/141‑1 (MQ=255) ttAGTATCTAATAAAATTTAATAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAatat > 2:490241/1‑141 (MQ=255) tAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTTTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAATGTGACATATCCATTATCTTTGTTAGTTAGAATCGTTGAGTTGCAATAtt > 2:158456/1‑141 (MQ=255) tAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATAtt > 3:420955/1‑141 (MQ=255) aGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTg > 4:76049/1‑141 (MQ=255) gTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt < 4:297315/141‑1 (MQ=255) gTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGt > 1:192089/1‑141 (MQ=255) cTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTAtgt < 2:598500/141‑1 (MQ=255) aTTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTgg < 1:33427/141‑1 (MQ=255) ttAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGa < 2:684360/141‑1 (MQ=255) | AAAGAACGCTTACCAATCTGGATAAAACAGTCAAAGCGAGCAATTCGAAACGACTAAAGGAACAGATAAATCGTACCGAAAATCATACAAAGGGTCTGAAATGAAAGTTTCTTAGACTATAAAAGAGATTAGTATCTATTAAATTTTATTAGATACTAATCTCTTTT‑T‑GTCTACGATAACGTAATATGATTGATTCTATTTACACGTACAAATGGTTTAAGGTGACATATCCATTATCTTTGTTAGATAGAATCGTTGATTTGCAATATTGTATGTGGA > USA300TCH1516_ALE/949950‑950228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |