Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 942,221 T→A 5.1% intergenic (‑192/+57) ptlE ← / ← mnhG1 Neopentalenolactone D synthase/Na(+)/H(+) antiporter subunit G1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE942,2210TA 5.1% 445.1 / 10.3 138intergenic (‑192/+57)ptlE/mnhG1Neopentalenolactone D synthase/Na(+)/H(+) antiporter subunit G1
Reads supporting (aligned to +/- strand):  ref base T (62/69);  new base A (3/4);  total (65/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 141 total aligned reads displayed.

TTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGC  >  USA300TCH1516_ALE/942081‑942361
                                                                                                                                            |                                                                                                                                             
ttatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAAttt                                                                                                                                               <  3:345500/141‑1 (MQ=255)
 tatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTg                                                                                                                                              <  1:491773/141‑1 (MQ=255)
   tCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACATCAGTAAAATCATTCCTAATTTGGa                                                                                                                                            >  2:432303/1‑141 (MQ=255)
   tCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGa                                                                                                                                            >  2:889310/1‑141 (MQ=255)
   tCATAATACATAACGAAATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGa                                                                                                                                            <  2:727559/141‑1 (MQ=255)
      taataaATAACGACATGAAAGGAAATTAAATTAAAGAAATATATGTAGATAGGGCGAATATAAAAAAAAAAAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc                                                                                                                                         <  4:220694/141‑1 (MQ=255)
      taataCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc                                                                                                                                         <  2:339109/141‑1 (MQ=255)
      taataCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc                                                                                                                                         <  2:224226/141‑1 (MQ=255)
      taataCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAAAAAAAAAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc                                                                                                                                         <  2:1057449/141‑1 (MQ=255)
            aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa                                                                                                                                   >  1:514018/1‑141 (MQ=255)
            aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa                                                                                                                                   <  3:215601/141‑1 (MQ=255)
            aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa                                                                                                                                   <  1:1082402/141‑1 (MQ=255)
             tAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc                                                                                                                                  <  1:984231/141‑1 (MQ=255)
             tAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc                                                                                                                                  <  1:1031661/141‑1 (MQ=255)
               aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTActg                                                                                                                               <  1:843732/141‑1 (MQ=38)
               aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg                                                                                                                               <  3:204046/141‑1 (MQ=255)
               aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActg                                                                                                                               <  3:106191/141‑1 (MQ=255)
               aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAACTTGGAATCATCTTActg                                                                                                                               >  2:712769/1‑141 (MQ=255)
                cGACATGAAAGGCAATTAAATTAAAGAGATATACGTAGATAGGGCGAAGATGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTActgc                                                                                                                              <  2:240075/141‑1 (MQ=25)
                 gACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAAGAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActgct                                                                                                                             <  4:447180/141‑1 (MQ=255)
                  aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCAATGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTActgctg                                                                                                                            <  2:978662/141‑1 (MQ=255)
                   cATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgt                                                                                                                           >  2:789001/1‑141 (MQ=255)
                    aTGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtt                                                                                                                          <  1:676320/141‑1 (MQ=255)
                     tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGTATCATCTTACTGCtgttt                                                                                                                         <  3:171914/141‑1 (MQ=255)
                     tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgttt                                                                                                                         <  4:17175/141‑1 (MQ=255)
                       aaaGGCAATTAAATTAATGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttgt                                                                                                                       <  1:875016/141‑1 (MQ=255)
                       aaaGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttgt                                                                                                                       <  3:445091/141‑1 (MQ=255)
                        aaGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCtgtttgtt                                                                                                                      >  1:713326/1‑141 (MQ=255)
                          ggCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgt                                                                                                                    >  1:316594/1‑141 (MQ=255)
                            cAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgttg                                                                                                                  >  1:421632/1‑141 (MQ=255)
                            cAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTttgttgttg                                                                                                                  <  1:1057559/141‑1 (MQ=255)
                              attaaattaaAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGAt                                                                                                                <  1:438344/141‑1 (MQ=255)
                                 aaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTta                                                                                                             >  1:728435/1‑141 (MQ=255)
                                  aattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTtat                                                                                                            >  2:577565/1‑141 (MQ=255)
                                      aaaGAGATATATGTAGATAGGGCGGATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTc                                                                                                        >  3:362076/1‑141 (MQ=255)
                                      aaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTCGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTc                                                                                                        <  1:783624/141‑1 (MQ=255)
                                        agagATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCAt                                                                                                      <  3:232366/141‑1 (MQ=255)
                                         gagaTATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATg                                                                                                     <  1:834337/141‑1 (MQ=255)
                                          agaTATATGTAAATAGGGAGAATCTGTAGTCAAAGAAAAAAAAAAAAAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGa                                                                                                    <  2:494589/141‑1 (MQ=255)
                                           gaTATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAt                                                                                                   >  1:386557/1‑141 (MQ=255)
                                           gaTATATGTAGATAAGGACAATCAGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAt                                                                                                   <  4:18749/141‑1 (MQ=255)
                                              atatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGAtttt                                                                                                >  1:780673/1‑141 (MQ=255)
                                              atatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTTCAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTg                                                                                               <  1:710419/141‑1 (MQ=255)
                                                 tGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCGTTAATACTTTGGCATGCAATAACATACTTTTATCGTGGATTGATATTGATGATTTTGtt                                                                                             >  1:1024620/1‑141 (MQ=255)
                                                         gggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc                                                                                     <  1:400991/141‑1 (MQ=255)
                                                         gggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc                                                                                     <  1:38745/141‑1 (MQ=255)
                                                          ggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCt                                                                                    <  1:402704/141‑1 (MQ=255)
                                                           gcgaagatGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa                                                                                   >  2:908027/8‑141 (MQ=255)
                                                           gCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa                                                                                   >  2:160187/1‑141 (MQ=255)
                                                             gAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACa                                                                                 >  1:976796/1‑141 (MQ=255)
                                                              aaTCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACaa                                                                                >  1:190458/1‑141 (MQ=255)
                                                               aTCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAt                                                                               >  1:115486/1‑141 (MQ=255)
                                                                tCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAtt                                                                              <  1:640092/141‑1 (MQ=255)
                                                                  tGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAtttt                                                                            <  1:1017159/141‑1 (MQ=255)
                                                                   gTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtg                                                                           <  2:265412/141‑1 (MQ=255)
                                                                   gTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtg                                                                           >  3:392516/1‑141 (MQ=255)
                                                                    tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                          <  1:909599/141‑1 (MQ=255)
                                                                    tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt                                                                          <  3:410490/141‑1 (MQ=255)
                                                                      gTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTgtgt                                                                        >  3:452388/1‑141 (MQ=255)
                                                                      gTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTgtgt                                                                        >  3:312289/1‑141 (MQ=255)
                                                                         aaaGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtt                                                                     <  2:1043715/141‑1 (MQ=255)
                                                                           aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt                                                                   >  4:359037/1‑141 (MQ=255)
                                                                            gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAatgat                                                                                                                                       <  4:162191/73‑4 (MQ=18)
                                                                            gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAatgat                                                                                                                                       >  3:162191/1‑70 (MQ=18)
                                                                            gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTa                                                                  >  1:253756/1‑141 (MQ=255)
                                                                                aaaTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTc                                                              >  1:743692/1‑141 (MQ=255)
                                                                                   tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc                                                           >  2:495792/1‑141 (MQ=255)
                                                                                   tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc                                                           <  4:21859/141‑1 (MQ=255)
                                                                                        gAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGaaa                                                      <  2:316328/141‑1 (MQ=255)
                                                                                          aaaaGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAAtt                                                    <  2:409371/141‑1 (MQ=255)
                                                                                            aaGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTc                                                  <  1:732965/141‑1 (MQ=255)
                                                                                             agagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCa                                                 <  4:58002/141‑1 (MQ=255)
                                                                                              gagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCAt                                                >  2:138730/1‑141 (MQ=255)
                                                                                              gagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCAt                                                >  2:138613/1‑141 (MQ=255)
                                                                                               agGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATc                                               >  2:392218/1‑141 (MQ=255)
                                                                                               agGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATc                                               >  2:84953/1‑141 (MQ=255)
                                                                                                 gTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGCATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGa                                             >  3:374343/1‑141 (MQ=255)
                                                                                                  tAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGAc                                            <  1:106482/141‑1 (MQ=255)
                                                                                                  tAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGAc                                            <  1:138433/141‑1 (MQ=255)
                                                                                                    aCAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACtt                                          >  2:192053/1‑141 (MQ=255)
                                                                                                      aaTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTACGAAATTTCATCGACTTTa                                        >  4:53259/1‑141 (MQ=255)
                                                                                                        tGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGt                                      <  1:577565/141‑1 (MQ=255)
                                                                                                            aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt                                  >  4:108889/1‑141 (MQ=255)
                                                                                                             aatgAAAACAGCAGTAAAATCACTCCTAATTTGGAATCAACTTAAAGCAGTTTGATGTTGATTTATATTCATTATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGATATTTCATCTACTTTAGTCtttt                                 >  2:945470/4‑141 (MQ=255)
                                                                                                               aGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTa                               >  1:986386/1‑141 (MQ=255)
                                                                                                                 aaaaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATCACTGATTTAGTTATATAATCTACACCTTTGTGCCTTTTAAGTCTTCCGAAATTTCATAGCCTTTAGTCTTTTTAGt                             >  3:223167/1‑141 (MQ=255)
                                                                                                                   aaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGtat                           <  1:557039/141‑1 (MQ=255)
                                                                                                                      agcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATATTCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAg                        >  4:109974/1‑141 (MQ=255)
                                                                                                                      agcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAg                        <  1:33092/141‑1 (MQ=255)
                                                                                                                       gcagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTCTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAgg                       <  1:800559/141‑1 (MQ=255)
                                                                                                                        cagTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGACATTTCATCGACTTTAGTCTTTTTAGTATAAGGc                      <  4:273109/141‑1 (MQ=255)
                                                                                                                           tAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtt                   <  2:70175/141‑1 (MQ=255)
                                                                                                                            aagatgaTTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGttt                  >  2:363288/7‑141 (MQ=38)
                                                                                                                            aaaaTCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGttt                  <  2:976796/141‑1 (MQ=255)
                                                                                                                             agatgaTTCCAAATTAGGAATGATTTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt                 >  4:266396/6‑141 (MQ=38)
                                                                                                                             aaaTCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt                 <  2:853860/141‑1 (MQ=255)
                                                                                                                                  aTTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAatat            >  2:823307/1‑141 (MQ=255)
                                                                                                                                   ttCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAAtatt           >  1:295269/1‑141 (MQ=255)
                                                                                                                                   ttCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAAtatt           <  2:583352/141‑1 (MQ=255)
                                                                                                                                           ttGGAATCATCTTACTGCTGTTTGTTGTTGATTTATCTTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGAGTTTTAATATTATAtgctg   >  4:320415/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTACTGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGC  >  USA300TCH1516_ALE/942081‑942361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: