Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 942,221 | T→A | 5.1% | intergenic (‑192/+57) | ptlE ← / ← mnhG1 | Neopentalenolactone D synthase/Na(+)/H(+) antiporter subunit G1 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 942,221 | 0 | T | A | 5.1% | 445.1 / 10.3 | 138 | intergenic (‑192/+57) | ptlE/mnhG1 | Neopentalenolactone D synthase/Na(+)/H(+) antiporter subunit G1 |
Reads supporting (aligned to +/- strand): ref base T (62/69); new base A (3/4); total (65/73) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 |
Only 100 of 141 total aligned reads displayed.
TTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGC > USA300TCH1516_ALE/942081‑942361 | ttatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAAttt < 3:345500/141‑1 (MQ=255) tatCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTg < 1:491773/141‑1 (MQ=255) tCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACATCAGTAAAATCATTCCTAATTTGGa > 2:432303/1‑141 (MQ=255) tCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGa > 2:889310/1‑141 (MQ=255) tCATAATACATAACGAAATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGa < 2:727559/141‑1 (MQ=255) taataaATAACGACATGAAAGGAAATTAAATTAAAGAAATATATGTAGATAGGGCGAATATAAAAAAAAAAAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc < 4:220694/141‑1 (MQ=255) taataCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc < 2:339109/141‑1 (MQ=255) taataCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc < 2:224226/141‑1 (MQ=255) taataCATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAAAAAAAAAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAatc < 2:1057449/141‑1 (MQ=255) aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa > 1:514018/1‑141 (MQ=255) aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa < 3:215601/141‑1 (MQ=255) aTAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTa < 1:1082402/141‑1 (MQ=255) tAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc < 1:984231/141‑1 (MQ=255) tAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc < 1:1031661/141‑1 (MQ=255) aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTAc‑tg < 1:843732/141‑1 (MQ=38) aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc‑tg < 3:204046/141‑1 (MQ=255) aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc‑tg < 3:106191/141‑1 (MQ=255) aCGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAACTTGGAATCATCTTAc‑tg > 2:712769/1‑141 (MQ=255) cGACATGAAAGGCAATTAAATTAAAGAGATATACGTAGATAGGGCGAAGATGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAATGATTTTAc‑tgc < 2:240075/141‑1 (MQ=25) gACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAAGAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc‑tgct < 4:447180/141‑1 (MQ=255) aCATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCAATGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAc‑tgctg < 2:978662/141‑1 (MQ=255) cATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCtgt > 2:789001/1‑141 (MQ=255) aTGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCtgtt < 1:676320/141‑1 (MQ=255) tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGTATCATCTTAC‑TGCtgttt < 3:171914/141‑1 (MQ=255) tGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCtgttt < 4:17175/141‑1 (MQ=255) aaaGGCAATTAAATTAATGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCtgtttgt < 1:875016/141‑1 (MQ=255) aaaGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCtgtttgt < 3:445091/141‑1 (MQ=255) aaGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCtgtttgtt > 1:713326/1‑141 (MQ=255) ggCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTttgttgt > 1:316594/1‑141 (MQ=255) cAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTttgttgttg > 1:421632/1‑141 (MQ=255) cAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTttgttgttg < 1:1057559/141‑1 (MQ=255) attaaattaaAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGAt < 1:438344/141‑1 (MQ=255) aaattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTta > 1:728435/1‑141 (MQ=255) aattaaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTtat > 2:577565/1‑141 (MQ=255) aaaGAGATATATGTAGATAGGGCGGATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTc > 3:362076/1‑141 (MQ=255) aaaGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTCGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTc < 1:783624/141‑1 (MQ=255) agagATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCAt < 3:232366/141‑1 (MQ=255) gagaTATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATg < 1:834337/141‑1 (MQ=255) agaTATATGTAAATAGGGAGAATCTGTAGTCAAAGAAAAAAAAAAAAAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGa < 2:494589/141‑1 (MQ=255) gaTATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGAt > 1:386557/1‑141 (MQ=255) gaTATATGTAGATAAGGACAATCAGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGAt < 4:18749/141‑1 (MQ=255) atatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGAtttt > 1:780673/1‑141 (MQ=255) atatGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGT‑TCAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTg < 1:710419/141‑1 (MQ=255) tGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCGTTAATACTTTGGCATGCAATAACATACT‑TTTATCGTGGATTGATATTGATGATTTTGtt > 1:1024620/1‑141 (MQ=255) gggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc < 1:400991/141‑1 (MQ=255) gggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATc < 1:38745/141‑1 (MQ=255) ggCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCt < 1:402704/141‑1 (MQ=255) gcgaagatGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa > 2:908027/8‑141 (MQ=255) gCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTa > 2:160187/1‑141 (MQ=255) gAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACa > 1:976796/1‑141 (MQ=255) aaTCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACaa > 1:190458/1‑141 (MQ=255) aTCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAt > 1:115486/1‑141 (MQ=255) tCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAtt < 1:640092/141‑1 (MQ=255) tGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAAtttt < 1:1017159/141‑1 (MQ=255) gTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtg < 2:265412/141‑1 (MQ=255) gTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtg > 3:392516/1‑141 (MQ=255) tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt < 1:909599/141‑1 (MQ=255) tAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTtgt < 3:410490/141‑1 (MQ=255) gTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTgtgt > 3:452388/1‑141 (MQ=255) gTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTgtgt > 3:312289/1‑141 (MQ=255) aaaGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtt < 2:1043715/141‑1 (MQ=255) aGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCtttt > 4:359037/1‑141 (MQ=255) gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAatgat < 4:162191/73‑4 (MQ=18) gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAGATGATTCCAAATTAGGAatgat > 3:162191/1‑70 (MQ=18) gAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTa > 1:253756/1‑141 (MQ=255) aaaTCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTc > 1:743692/1‑141 (MQ=255) tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc > 2:495792/1‑141 (MQ=255) tCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTc < 4:21859/141‑1 (MQ=255) gAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGaaa < 2:316328/141‑1 (MQ=255) aaaaGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAAtt < 2:409371/141‑1 (MQ=255) aaGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTc < 1:732965/141‑1 (MQ=255) agagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCa < 4:58002/141‑1 (MQ=255) gagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCAt > 2:138730/1‑141 (MQ=255) gagGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCAt > 2:138613/1‑141 (MQ=255) agGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATc > 2:392218/1‑141 (MQ=255) agGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATc > 2:84953/1‑141 (MQ=255) gTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGCATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGa > 3:374343/1‑141 (MQ=255) tAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGAc < 1:106482/141‑1 (MQ=255) tAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGAc < 1:138433/141‑1 (MQ=255) aCAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACtt > 2:192053/1‑141 (MQ=255) aaTGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTACGAAATTTCATCGACTTTa > 4:53259/1‑141 (MQ=255) tGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGt < 1:577565/141‑1 (MQ=255) aaaaGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCttt > 4:108889/1‑141 (MQ=255) aatgAAAACAGCAGTAAAATCACTCCTAATTTGGAATCAACTTAA‑AGCAGTTTGATGTTGATTTATATTCATTATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGATATTTCATCTACTTTAGTCtttt > 2:945470/4‑141 (MQ=255) aGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTa > 1:986386/1‑141 (MQ=255) aaaaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATCACTGATTTAGTTATATAATCTACACCTTTGTGCCTTTTAAGTCTTCCGAAATTTCATAGCCTTTAGTCTTTTTAGt > 3:223167/1‑141 (MQ=255) aaCAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGtat < 1:557039/141‑1 (MQ=255) agcagTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATATTCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAg > 4:109974/1‑141 (MQ=255) agcagTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAg < 1:33092/141‑1 (MQ=255) gcagTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTCTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAgg < 1:800559/141‑1 (MQ=255) cagTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGACATTTCATCGACTTTAGTCTTTTTAGTATAAGGc < 4:273109/141‑1 (MQ=255) tAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtt < 2:70175/141‑1 (MQ=255) aagatgaTTCCAAATTAGGAATGATTTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGttt > 2:363288/7‑141 (MQ=38) aaaaTCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGttt < 2:976796/141‑1 (MQ=255) agatgaTTCCAAATTAGGAATGATTTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt > 4:266396/6‑141 (MQ=38) aaaTCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGtttt < 2:853860/141‑1 (MQ=255) aTTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAatat > 2:823307/1‑141 (MQ=255) ttCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAAtatt > 1:295269/1‑141 (MQ=255) ttCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAAtatt < 2:583352/141‑1 (MQ=255) ttGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATCTTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGAGTTTTAATATTATAtgctg > 4:320415/1‑141 (MQ=255) | TTATCATAATACATAACGACATGAAAGGCAATTAAATTAAAGAGATATATGTAGATAGGGCGAATCTGTAGTCAAAGAAAAAATCATTGAAAAAGAGGTAACAATGTCAAAAGAAAACAGCAGTAAAATCATTCCTAATTTGGAATCATCTTAC‑TGCTGTTTGTTGTTGATTTATATTCATGATTTTGTTATATAATCTACAATTTTGTGTCTTTTAAGTCTTCCGAAATTTCATCGACTTTAGTCTTTTTAGTATAAGGCGTTTTAATATTATATGCTGC > USA300TCH1516_ALE/942081‑942361 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |