Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 913,454 C→A 8.6% G293G (GGC→GGA)  sufB_1 → FeS cluster assembly protein SufB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE913,4540CA 8.6% 387.7 / 7.9 124G293G (GGC→GGA) sufB_1FeS cluster assembly protein SufB
Reads supporting (aligned to +/- strand):  ref base C (67/44);  new base A (2/11);  total (69/55)
Fisher's exact test for biased strand distribution p-value = 2.56e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GGTATTACTGAAGCGGATGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCATGGTGGAACTAA  >  USA300TCH1516_ALE/913317‑913594
                                                                                                                                         |                                                                                                                                            
ggTATTACTGAAGCGGATGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGaa                                                                                                                                           >  1:208006/1‑141 (MQ=255)
 gTATTACTGAAGCGGATGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAAc                                                                                                                                          <  3:444461/141‑1 (MQ=255)
        tGAAGCGGATGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAAt                                                                                                                                   >  4:368434/1‑141 (MQ=255)
            gCGGATGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAAt                                                                                                                               <  2:320237/141‑1 (MQ=255)
             cGGATGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATc                                                                                                                              >  3:437835/1‑141 (MQ=255)
                 tGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGACAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAAc                                                                                                                          >  1:818754/1‑141 (MQ=255)
                 agCCTCAATTAATTGGACACAAGGTATAATGAATGAAGCTATGCAAATTATTGATAAGACAACAAATTTATTTAGTGATCGTTAAACAAGTACACTTAAATCAGTAGTTGTAGTGAAAAAAAAAAAAAAAATTAATCTAAc                                                                                                                          <  4:340594/140‑1 (MQ=255)
                  gCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACa                                                                                                                         >  1:352349/1‑141 (MQ=255)
                   ccTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCCGTAGTTGTAGGTACAGGCGAACAAAAAATTAATATAACAt                                                                                                                        >  3:428268/1‑141 (MQ=255)
                   ccTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACAt                                                                                                                        >  2:438540/1‑141 (MQ=255)
                   ccTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGATGTAGGTACAGGCGAACAAAAAATTAATCTAACAt                                                                                                                        >  1:947547/1‑141 (MQ=255)
                   ccTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATATATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACAt                                                                                                                        >  2:520745/1‑141 (MQ=255)
                     tCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCt                                                                                                                      <  1:519833/141‑1 (MQ=255)
                     tCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCt                                                                                                                      <  2:744418/141‑1 (MQ=255)
                     tCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCt                                                                                                                      <  2:308908/141‑1 (MQ=255)
                     tCAATTAATTGAGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCAAACAAAAAATTAATCTAACATCt                                                                                                                      <  2:325998/141‑1 (MQ=255)
                           aattGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATc                                                                                                                <  2:835548/141‑1 (MQ=255)
                            attGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCg                                                                                                               <  4:272195/141‑1 (MQ=255)
                            aaagggCACTAGGTTTAATGAATAAGGGTAGAAAAAGTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGAAAAAAAAAAAAAAAAATTAATCTAACATCTAAAATCg                                                                                                               <  2:472427/138‑1 (MQ=255)
                              tgagCACTAGGTTTAATGAATGAGGGAAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTAAAAAAAAAAAAAAAATTAATCTAACATCTAAAATCGTa                                                                                                             <  2:132268/138‑1 (MQ=255)
                              tGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACACATTTATTGGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGCCGAACAAAAAATTAATCTAACATCTAAAATCGTa                                                                                                             >  2:243432/1‑141 (MQ=255)
                              tGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTa                                                                                                             >  2:598265/1‑141 (MQ=255)
                                ggCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACa                                                                                                           >  1:663201/1‑141 (MQ=255)
                                ggCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACa                                                                                                           <  1:331650/141‑1 (MQ=255)
                                ggCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACa                                                                                                           >  2:708887/1‑141 (MQ=255)
                                   aCTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAata                                                                                                        <  3:105139/141‑1 (MQ=255)
                                      aGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATgg                                                                                                     >  1:931007/1‑141 (MQ=255)
                                      aGGTTTAATGAATGAGGGTAGCCAAATTATGGAGAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGAAAAAAAAAAAAAAAAATTAATCTAACATCTAAAATCGTACAATATgg                                                                                                     <  2:321468/141‑1 (MQ=255)
                                           tAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAg                                                                                                >  1:606318/1‑141 (MQ=255)
                                            aatgaatgGGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGa                                                                                               <  2:556565/141‑1 (MQ=255)
                                             atgaatgaGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGaa                                                                                              >  2:798027/1‑141 (MQ=255)
                                             atgaatgaGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGaa                                                                                              >  1:180628/1‑141 (MQ=255)
                                              tgaatgaGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGaaa                                                                                             <  2:871491/141‑1 (MQ=255)
                                              tgaatgaGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGaaa                                                                                             <  2:886730/141‑1 (MQ=255)
                                              tgaatgaGAGTAGCAAAATTATTGATAATAAAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGTCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGaaa                                                                                             <  2:803009/141‑1 (MQ=255)
                                                 atgaGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAg                                                                                          >  1:331893/1‑141 (MQ=255)
                                                    aGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCTAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATg                                                                                       <  1:262035/141‑1 (MQ=255)
                                                    aGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATg                                                                                       <  3:151446/141‑1 (MQ=255)
                                                     gggTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGAGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATgg                                                                                      >  2:799685/1‑141 (MQ=255)
                                                      ggTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAATAGCGTACAATATGGTAAAGAAACAGATGGt                                                                                     <  2:692446/141‑1 (MQ=255)
                                                      ggTAACCAAATTATAGATAAAACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGAAAAAAAAAAAAAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGt                                                                                     <  2:1064339/141‑1 (MQ=255)
                                                       gTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGtt                                                                                    >  4:244987/1‑141 (MQ=255)
                                                         aGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTtat                                                                                  >  1:812771/1‑141 (MQ=255)
                                                          gCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTtata                                                                                 <  1:475342/141‑1 (MQ=255)
                                                          gCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGAAAAAAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTtata                                                                                 <  2:719370/141‑1 (MQ=255)
                                                             aaaTTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATcc                                                                              <  1:569685/141‑1 (MQ=255)
                                                             aaaTTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGGAAAAAAAAAAAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATcc                                                                              <  2:578394/141‑1 (MQ=255)
                                                              aaTTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCt                                                                             >  3:295925/1‑141 (MQ=255)
                                                              aaTTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTAAAAAAAAAAAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCt                                                                             <  2:399288/141‑1 (MQ=255)
                                                                   ttGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAAACAAAAAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAAc                                                                        <  2:1066452/141‑1 (MQ=255)
                                                                    tGATAATACAACAAATTTATTTGGTGCGGGTTCAGCAGGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACa                                                                       >  4:363413/1‑141 (MQ=255)
                                                                    tGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTACAATCGTACAATATGGTAAAGAAACAGATAGTTATATCCTTAAACa                                                                       >  2:1004131/1‑141 (MQ=255)
                                                                    tGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACa                                                                       >  2:871638/1‑141 (MQ=255)
                                                                    tGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACa                                                                       >  3:344801/1‑141 (MQ=255)
                                                                     gATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACAt                                                                      <  3:368434/141‑1 (MQ=255)
                                                                     gATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACAt                                                                      >  1:49962/1‑141 (MQ=255)
                                                                       taataCAGCAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGATACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATgg                                                                    <  2:1002179/141‑1 (MQ=255)
                                                                        aataCAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGgt                                                                   <  1:481206/141‑1 (MQ=255)
                                                                            caacaaATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTa                                                               >  2:889455/1‑141 (MQ=255)
                                                                               caaATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGa                                                            >  2:511001/1‑141 (MQ=255)
                                                                                   tttatttGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGa                                                        <  2:528528/141‑1 (MQ=255)
                                                                                   tttatttGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGa                                                        <  2:818147/141‑1 (MQ=255)
                                                                                   tttatttGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGAGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGa                                                        >  3:159451/1‑141 (MQ=255)
                                                                                      atttGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACa                                                     >  2:96199/1‑141 (MQ=255)
                                                                                      atctGGGGATCGTCCCACCAGTTCACCGAAAGCAGTAGTCGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTACATCCTTAAACAGGGTGTTATGAAAGACCa                                                     >  2:526533/4‑141 (MQ=255)
                                                                                       tttGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTAAAAAAAAAAAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACAt                                                    <  2:230831/141‑1 (MQ=255)
                                                                                       tttGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAAGTAAAGGAGAACAAAAAATTAATATAACGTCTAAAATCGTAAAATATGGTAAAGAAACATATGGTTATTTCCTTATACATTGTGTTATGAAAGAACAt                                                    <  4:40716/141‑1 (MQ=255)
                                                                                         tGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATACAAAGGCTAACAATATGGTAAAAAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGc                                                  >  3:317026/1‑141 (MQ=255)
                                                                                           gTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCAt                                                >  1:218331/1‑141 (MQ=255)
                                                                                               tCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTc                                            <  2:616167/141‑1 (MQ=255)
                                                                                               tCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTc                                            <  1:650889/141‑1 (MQ=255)
                                                                                                cGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCt                                           <  4:229687/141‑1 (MQ=255)
                                                                                                 gTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTg                                          <  1:645661/141‑1 (MQ=255)
                                                                                                     aacaaGTTCACTTAAATCAGTAGTTGTAGGTACAGGCTAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTAtt                                      >  1:1009661/1‑141 (MQ=255)
                                                                                                           ttCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATgg                                >  2:425416/1‑141 (MQ=255)
                                                                                                              aCTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTAt                             >  1:265286/1‑141 (MQ=255)
                                                                                                              aCTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTAt                             >  2:281230/1‑141 (MQ=255)
                                                                                                                ttAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCg                           >  1:566377/1‑141 (MQ=255)
                                                                                                                ttAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCg                           >  4:401302/1‑141 (MQ=255)
                                                                                                                 tAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCgg                          <  4:336651/141‑1 (MQ=255)
                                                                                                                  aaaTCAGTAGTTGTAGGTACAGGAGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCATCTGTATTTAATGGTATCGGc                         <  1:799685/141‑1 (MQ=255)
                                                                                                                   aaTCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATGTAACGTGTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGGATTTAACGGTTTCGGCt                        >  2:163323/1‑141 (MQ=255)
                                                                                                                   aaTCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCt                        >  1:445639/1‑141 (MQ=255)
                                                                                                                    aTCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTa                       >  2:678464/1‑141 (MQ=255)
                                                                                                                    aTCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTa                       >  1:30892/1‑141 (MQ=255)
                                                                                                                       agtagtTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACAt                    >  2:463888/1‑141 (MQ=255)
                                                                                                                       agtagtTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACAt                    <  2:667397/141‑1 (MQ=255)
                                                                                                                        gtagtTGTAGGTACAGGCGCACAAAAAACTAATCTAATATCCAAAAACTTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACAtt                   <  3:199188/141‑1 (MQ=255)
                                                                                                                        gtagtTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACAtt                   <  2:802819/141‑1 (MQ=255)
                                                                                                                         tagtTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTa                  <  2:723699/141‑1 (MQ=255)
                                                                                                                           gtcgTAGGTACAGGCGAACAAAAAATTACTCTAACATACAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAg                >  2:247532/4‑141 (MQ=255)
                                                                                                                             tGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCa              >  1:310415/1‑141 (MQ=255)
                                                                                                                                 ggTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCAtggt          <  1:116527/141‑1 (MQ=255)
                                                                                                                                 ggTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCAtggt          >  3:170151/1‑141 (MQ=255)
                                                                                                                                  gTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCAtggtg         >  4:100112/1‑141 (MQ=255)
                                                                                                                                  gTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCAtggtg         >  2:479294/1‑141 (MQ=255)
                                                                                                                                  gTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCAtggtg         >  1:143850/1‑141 (MQ=255)
                                                                                                                                     cAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCATGGTGGaa      >  2:44129/1‑141 (MQ=255)
                                                                                                                                       ggCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCATGGTGGAACt    >  4:453066/1‑141 (MQ=255)
                                                                                                                                         cGAACAAAAAATTAATCTAACATCTAAAGTCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCATGGTGGAACTaa  <  4:236210/141‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
GGTATTACTGAAGCGGATGCCTCAATTAATTGGGCACTAGGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGTTCAACAAGTTCACTTAAATCAGTAGTTGTAGGTACAGGCGAACAAAAAATTAATCTAACATCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATGAAAGAACATGCATCGTCTGTATTTAATGGTATCGGCTACATTAAGCATGGTGGAACTAA  >  USA300TCH1516_ALE/913317‑913594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑