Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 895,369 A→G 5.3% A130A (GCA→GCG metQ_2 → Methionine‑binding lipoprotein MetQ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE895,3690AG 5.3% 393.4 / 8.6 131A130A (GCA→GCGmetQ_2Methionine‑binding lipoprotein MetQ
Reads supporting (aligned to +/- strand):  ref base A (73/50);  new base G (4/3);  total (78/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

Only 100 of 137 total aligned reads displayed.

TATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATAT  >  USA300TCH1516_ALE/895229‑895509
                                                                                                                                            |                                                                                                                                            
tATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCa                                                                                                                                              <  2:7093/141‑1 (MQ=255)
tATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCa                                                                                                                                              <  2:356774/141‑1 (MQ=255)
  tttCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAAc                                                                                                                                            <  2:949063/141‑1 (MQ=255)
   ttCCAAAATACACCATATTAAAACACAGAGAAAACGGATAAAGGTTACAAGATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTCTACACTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACa                                                                                                                                           >  2:105020/1‑141 (MQ=255)
     ccAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGt                                                                                                                                         <  1:315940/141‑1 (MQ=255)
     ccAACAGACACCATAGTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGGGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTGAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGt                                                                                                                                         <  1:897905/141‑1 (MQ=255)
      cAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTc                                                                                                                                        >  1:131139/1‑141 (MQ=255)
          aTACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTAtg                                                                                                                                    >  4:22938/1‑141 (MQ=255)
            acacCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTAtgtg                                                                                                                                  >  2:988394/1‑141 (MQ=255)
             cacCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATgtgt                                                                                                                                 >  1:25271/1‑141 (MQ=255)
               ccATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCt                                                                                                                               >  2:139743/1‑141 (MQ=255)
                   atTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCtaata                                                                                                                           <  2:290899/141‑1 (MQ=255)
                     ttAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTaataat                                                                                                                         >  1:1084786/1‑141 (MQ=255)
                     ttAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTaataat                                                                                                                         >  3:12390/1‑141 (MQ=255)
                      tAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATc                                                                                                                        <  1:544759/141‑1 (MQ=255)
                      tAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCGAAAAAGTATAAAAGATTAAAAGAATTACAAAAAGGTGCAACAATATATGTGTCTaataata                                                                                                                        >  4:415506/1‑140 (MQ=255)
                         acacAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAg                                                                                                                     >  1:441827/1‑141 (MQ=255)
                         acacAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAg                                                                                                                     >  2:439336/1‑141 (MQ=255)
                         acacAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAg                                                                                                                     >  3:319387/1‑141 (MQ=255)
                         acacAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCGAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAg                                                                                                                     >  3:317587/1‑141 (MQ=255)
                           acaGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCt                                                                                                                   >  4:351106/1‑141 (MQ=255)
                              gagaAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGaa                                                                                                                >  2:472205/1‑141 (MQ=255)
                              gagaAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGaa                                                                                                                >  2:472189/1‑141 (MQ=255)
                                gaAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACa                                                                                                              >  3:400536/1‑141 (MQ=255)
                                  aaaaGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAg                                                                                                            >  3:126942/1‑141 (MQ=255)
                                  aaaaGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAg                                                                                                            <  3:252140/141‑1 (MQ=255)
                                    aaGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGa                                                                                                          >  3:273355/1‑141 (MQ=255)
                                    aaGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGa                                                                                                          >  4:123073/1‑141 (MQ=255)
                                      ggATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACg                                                                                                        >  2:71672/1‑141 (MQ=255)
                                       gATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGt                                                                                                       <  1:975331/141‑1 (MQ=255)
                                       gATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGt                                                                                                       <  2:150477/141‑1 (MQ=255)
                                        aTAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGtt                                                                                                      >  1:692687/1‑141 (MQ=255)
                                         tAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGttt                                                                                                     <  4:402945/141‑1 (MQ=255)
                                            aGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTCTCtt                                                                                                  >  2:1022750/1‑141 (MQ=255)
                                               ttACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaa                                                                                               <  2:1049500/141‑1 (MQ=255)
                                                tACAAAATCGAAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCCAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTaaaa                                                                                              <  4:409907/141‑1 (MQ=255)
                                                 aCAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAt                                                                                             <  1:348061/141‑1 (MQ=255)
                                                     aaTCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAttctt                                                                                         >  4:250376/1‑141 (MQ=255)
                                                       tCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCg                                                                                       <  3:133473/141‑1 (MQ=255)
                                                             gTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATg                                                                                 <  4:328421/141‑1 (MQ=255)
                                                              tGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGc                                                                                >  2:775388/1‑141 (MQ=255)
                                                                 cGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAgg                                                                             <  3:269020/141‑1 (MQ=255)
                                                                  ggTGCTGTTCAATTAGCACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGGATTTCCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGACCAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGt                                                                            <  2:28181/141‑1 (MQ=255)
                                                                  ggTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGt                                                                            <  1:290776/141‑1 (MQ=255)
                                                                   gTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGtt                                                                           >  2:1041287/1‑141 (MQ=255)
                                                                   gTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGtt                                                                           <  1:375263/141‑1 (MQ=255)
                                                                    tGATGTTCACTTAGAACGTAGTGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGttt                                                                          <  3:24345/141‑1 (MQ=255)
                                                                     gATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTtta                                                                         >  2:46274/1‑141 (MQ=255)
                                                                       tGTTGACGTAGAACCTATGGCTGTATACTCGAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAAGTCTTCGTTGATGCAGGTttaat                                                                       <  2:553772/141‑1 (MQ=255)
                                                                           cACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTaaa                                                                   >  2:260025/1‑141 (MQ=255)
                                                                           cACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTaaa                                                                   <  3:301387/141‑1 (MQ=255)
                                                                            aCTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTttaantaaga                                                                  >  3:161075/1‑139 (MQ=255)
                                                                             cTTAGAACCTATGGCTGTATACTCTACAAAGTATACAAGTTTAAAAGAATTACCAAAAGGCGCCACAGCCTATGTGTCTACTAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGATTACTTAAAAt                                                                 >  4:295830/1‑141 (MQ=255)
                                                                             cTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAAt                                                                 >  2:607189/1‑141 (MQ=255)
                                                                              ttAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATc                                                                <  1:902916/141‑1 (MQ=255)
                                                                              ttAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATc                                                                <  1:653388/141‑1 (MQ=255)
                                                                                 gAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaa                                                             >  3:454473/1‑141 (MQ=255)
                                                                                   aCCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaaaa                                                           >  2:376171/1‑141 (MQ=255)
                                                                                   aCCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCaaaaa                                                           >  3:253703/1‑141 (MQ=255)
                                                                                     cTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAg                                                         >  2:727863/1‑141 (MQ=255)
                                                                                        tGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCg                                                      >  2:826428/1‑141 (MQ=255)
                                                                                        gggCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCg                                                      <  2:553908/140‑1 (MQ=255)
                                                                                         ggCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGt                                                     >  1:523080/1‑141 (MQ=255)
                                                                                          gCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTa                                                    <  1:17805/141‑1 (MQ=255)
                                                                                           cTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTaa                                                   >  1:1050325/1‑141 (MQ=255)
                                                                                              tataCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAAAAAAAAAAAAAAAAGGCGTaaaaa                                                <  4:140599/141‑1 (MQ=255)
                                                                                              tataCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAAAAAAAAAAAAAAAAGGCGTaaaaa                                                <  4:441071/141‑1 (MQ=255)
                                                                                               ataCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACGAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAAt                                               >  1:175522/1‑141 (MQ=255)
                                                                                                taCTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAAtt                                              >  4:72823/1‑141 (MQ=255)
                                                                                                  atctAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAAACAAAAAAGGCGTAAAAATTGa                                            <  2:36008/140‑1 (MQ=255)
                                                                                                  atctAAAAAGTATAAAAGATTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAAAAAAAAAAAAAAAAGGCGTAAAAATTGa                                            <  2:766363/140‑1 (MQ=255)
                                                                                                     tAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGa                                         >  1:659909/1‑141 (MQ=255)
                                                                                                     tAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCGTAAAATTCTTAGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAGGa                                         <  4:21271/141‑1 (MQ=255)
                                                                                                       aaaaGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATg                                       >  1:796222/1‑141 (MQ=255)
                                                                                                         aaGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCt                                     <  4:299649/141‑1 (MQ=255)
                                                                                                          aGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTa                                    <  3:158464/141‑1 (MQ=255)
                                                                                                          aGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTa                                    <  1:868670/141‑1 (MQ=255)
                                                                                                            tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCta                                                                                                                              >  2:57934/1‑49 (MQ=255)
                                                                                                            tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCta                                                                                                                              <  1:57934/49‑1 (MQ=255)
                                                                                                            tataAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAg                                  >  3:197460/1‑141 (MQ=255)
                                                                                                              taAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGtt                                >  1:54271/1‑141 (MQ=255)
                                                                                                              taAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGtt                                >  2:845382/1‑141 (MQ=255)
                                                                                                               aaaaGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGttt                               >  3:20391/1‑141 (MQ=255)
                                                                                                                aaaGTTTAAAAGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTa                              <  4:164807/141‑1 (MQ=255)
                                                                                                                aaaGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTa                              >  2:861380/1‑141 (MQ=255)
                                                                                                                 aaGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAg                             >  4:79915/1‑141 (MQ=255)
                                                                                                                     ttAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGat                         >  1:445987/1‑141 (MQ=255)
                                                                                                                         aaGAATTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTa                     >  1:191442/1‑141 (MQ=255)
                                                                                                                            aaTTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACag                  <  1:337158/141‑1 (MQ=255)
                                                                                                                             aTTACCAAAAGGTGCGACAGTTTATGTGTCTAATAATCCAGCTGAACAAGGACGCTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAGATCAAAAAAGACGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACaga                 >  1:801885/1‑141 (MQ=255)
                                                                                                                             aTTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACaga                 >  1:70056/1‑141 (MQ=255)
                                                                                                                              ttACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                >  3:143763/1‑141 (MQ=255)
                                                                                                                              ttACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTCAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                <  2:1004628/141‑1 (MQ=255)
                                                                                                                              ttACCAAAAGGTGCAACAGTCTATGTGTCTAAGAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACagag                <  1:236569/141‑1 (MQ=255)
                                                                                                                                    aaaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaa          >  2:636033/1‑141 (MQ=255)
                                                                                                                                     aaGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATaaaa         >  1:252289/1‑141 (MQ=255)
                                                                                                                                          gCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGat    >  1:768060/1‑141 (MQ=255)
                                                                                                                                            aaCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGatat  <  3:273210/141‑1 (MQ=255)
                                                                                                                                            aaCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGatat  <  2:438386/141‑1 (MQ=255)
                                                                                                                                            aaCAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGatat  <  2:718702/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TATTTCCAACATACACCATATTTAAACACAGAGAAAAAGGATAAAGGTTACAAAATCGTAAGTGCCGGTGATGTTCACTTAGAACCTATGGCTGTATACTCTAAAAAGTATAAAAGTTTAAAAGAATTACCAAAAGGTGCAACAGTCTATGTGTCTAATAATCCAGCTGAACAAGGACGTTTCTTAAAATTCTTCGTTGATGCAGGTTTAATTAAAATCAAAAAAGGCGTAAAAATTGAAGATGCTAAGTTTAGTGATATTACAGAGAATAAAAAAGATAT  >  USA300TCH1516_ALE/895229‑895509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: