Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 853,045 | A→G | 5.1% | intergenic (+52/+191) | clpP_1 → / ← USA300HOU_RS04170 | ATP‑dependent Clp protease proteolytic subunit/Epimerase family protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 853,045 | 0 | A | G | 5.1% | 456.8 / 9.3 | 151 | intergenic (+52/+191) | clpP_1/USA300HOU_RS04170 | ATP‑dependent Clp protease proteolytic subunit/Epimerase family protein |
Reads supporting (aligned to +/- strand): ref base A (69/74); new base G (4/4); total (73/78) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 155 total aligned reads displayed.
ACACAGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAG > USA300TCH1516_ALE/852908‑853185 | caGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCGTTTTGTATGAGTAAACCAAGgtgt < 1:261554/141‑1 (MQ=255) caGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCATAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTACAGAGCCAACTGAGCTGTCTGCTATTTTTGTATGAGTAAACGAAGgtgt > 1:10261/1‑141 (MQ=255) aGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTc < 2:330764/141‑1 (MQ=255) aGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTc > 2:753912/1‑141 (MQ=255) gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa > 2:1003195/1‑141 (MQ=255) gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa < 1:820871/141‑1 (MQ=255) gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa < 3:387141/141‑1 (MQ=255) gATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCa < 4:110569/141‑1 (MQ=255) gATCGTGATAACTTCTTAACTGCAGAAGAAACTAAAGAATATGGCTTAATTGACGAAGTGATGGTACTTGAAACAAAATAATTAAAAGTAAAGAGTAGACTACGCTGTCTGCACTTTTTGTATGAGTAAACGAAGGTGTCa > 4:234765/1‑141 (MQ=255) cGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCaata > 1:641070/1‑141 (MQ=255) tGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCaataat < 3:114274/141‑1 (MQ=255) gATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAAtt > 3:17290/1‑141 (MQ=255) tcttAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTAc > 1:128832/1‑141 (MQ=255) cttAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACt < 2:504305/141‑1 (MQ=255) cttAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACt > 2:724271/1‑141 (MQ=255) cttAACTGCAGAAGAAGCTAAAGAATATGGCCTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCGTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACt > 3:111614/1‑141 (MQ=255) gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt > 4:220757/1‑141 (MQ=255) gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt > 3:50601/1‑141 (MQ=255) gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt > 1:80504/1‑141 (MQ=255) gCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACttt > 3:246608/1‑141 (MQ=255) cAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTg < 4:304632/141‑1 (MQ=255) agaagaAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGa > 1:601307/1‑141 (MQ=255) aagaagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGc > 4:368727/1‑141 (MQ=255) aagaagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGc > 1:977386/1‑141 (MQ=255) aagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGa < 2:508083/141‑1 (MQ=255) aagCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCAGTCTGCTCTTTTTGTATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTCGAGCGGa < 3:402135/141‑1 (MQ=255) cTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAat < 2:79046/141‑1 (MQ=255) tAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAata < 2:152297/141‑1 (MQ=255) gaaaGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAata < 2:968887/140‑1 (MQ=255) gAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGAt < 2:512367/141‑1 (MQ=255) aaTATGGCTTAATTGATGAAGTGATGGTACCCGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGAtt > 1:271387/1‑141 (MQ=255) atGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaa > 1:413432/1‑141 (MQ=255) tGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaac < 3:273712/141‑2 (MQ=255) ggCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatg > 1:552331/1‑141 (MQ=255) gCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatga > 1:560565/1‑141 (MQ=255) tAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGc < 2:1051452/141‑1 (MQ=255) gatgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGtt < 1:701115/141‑1 (MQ=255) atgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTg > 1:475706/1‑141 (MQ=255) atgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTg > 1:98013/1‑141 (MQ=255) tgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGa > 1:1047094/1‑141 (MQ=255) tgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGa < 1:510088/141‑1 (MQ=255) tgaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGa > 2:556774/1‑141 (MQ=255) gaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGaa > 2:606212/1‑141 (MQ=255) gaAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGaa > 1:898699/1‑141 (MQ=255) tGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAAc < 2:4590/141‑1 (MQ=255) tGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAAc < 1:279689/141‑1 (MQ=255) tACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACtat > 2:580581/1‑141 (MQ=255) tACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACtat < 1:614303/141‑1 (MQ=255) aCCTGAAATAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACtata > 2:525578/1‑141 (MQ=255) tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa < 2:315545/141‑1 (MQ=255) tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa < 1:358846/141‑1 (MQ=255) tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa < 3:191216/141‑1 (MQ=255) tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa < 4:113390/141‑1 (MQ=255) tGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGa < 3:369344/141‑1 (MQ=255) gAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGaa < 1:755445/141‑1 (MQ=255) aaaCAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGaaa < 1:199934/141‑1 (MQ=255) aaCAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatg > 1:590401/1‑112 (MQ=255) aaCAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATtgaatg < 2:590401/112‑1 (MQ=255) aCAAAATAATTCAAAGTAAAGAGTAGACTAAGCAGTCTGCTCTTTTTGTATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATg > 4:425015/1‑141 (MQ=255) cAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGt > 1:750066/1‑141 (MQ=255) aaaTAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTCACTATATGAAATGTTc > 3:324783/1‑141 (MQ=255) cAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCaaa < 2:833388/141‑1 (MQ=255) gTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAg < 1:343663/141‑1 (MQ=255) gTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAg < 4:276109/141‑1 (MQ=255) tAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGa < 1:183230/141‑1 (MQ=255) aaGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACa > 1:512847/1‑141 (MQ=255) agagTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCGAGGTGTTAATAATTTGTTTACTATATTTTGAGCGGCAATATGATTGAATGAAGCTAGTTGAACCCTAACTGTTTTAGATGTTACCTTCTAAGTAGCCAt > 2:286980/1‑141 (MQ=255) agagTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACAt < 2:437810/141‑1 (MQ=255) gagTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAAGTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTATACCtt > 1:141612/1‑141 (MQ=255) agTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTg < 2:244567/141‑1 (MQ=255) aCTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAgg < 1:455020/141‑1 (MQ=255) cTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGa > 1:489920/1‑141 (MQ=255) gCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGaaatgttcct < 1:1040050/141‑8 (MQ=255) gTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTc < 2:315847/141‑1 (MQ=255) tCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCa < 3:226409/141‑1 (MQ=255) gCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATc < 2:1010970/141‑1 (MQ=255) actTTTTTTATTAGTAAACTAAGTAGTCAATAATATATTTATTATACTTTGTGCGGAAAGATGAAGTAATGCATCTAGTTGAACCGTAACTATTTGAAATATTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCt > 2:687005/2‑141 (MQ=255) ctTTTTGTATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtt < 1:216284/141‑1 (MQ=255) ttttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttg < 4:61005/141‑1 (MQ=255) ttttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttg < 2:1064669/141‑1 (MQ=255) gtttGTGTGAGTACACCAAGGTAACAATCCTTCGTTTACTCTACTTTGAGCGGGAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGACGGTAACATTTCAATCCtttg > 2:517643/2‑141 (MQ=255) tttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttgt > 1:944682/1‑141 (MQ=255) ttGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttgtt < 1:134010/141‑1 (MQ=255) tGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCTTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCtttgttt < 2:267691/141‑1 (MQ=255) tATGAGTAAACCGAGGTGTCAATAAATTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTttgtttgt < 2:958606/141‑1 (MQ=255) tATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTttgtttgt > 2:172426/1‑141 (MQ=255) tATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTttgtttgt > 1:1052244/1‑141 (MQ=255) aTGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTa > 3:299048/1‑141 (MQ=255) aTGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTa > 1:512837/1‑141 (MQ=255) tGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTaa < 1:263047/141‑1 (MQ=255) tGAGTAAACCAAGCTGTCAATAATTTGTTTTCTCTACTTTTAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGACATGTTCCCTTCAAAGTATACATTGAAAGGAACATTTCAATCCTTTGTTTGCaa > 2:864857/1‑141 (MQ=255) gAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAg < 2:896163/141‑1 (MQ=255) gAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAg > 2:415250/1‑141 (MQ=255) tAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCg > 1:1011462/1‑141 (MQ=255) tAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCg > 2:339760/1‑141 (MQ=255) tAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTGGTTTGTAAGTCg > 1:147668/1‑141 (MQ=255) aaaCCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGc < 2:977386/141‑1 (MQ=255) cAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTa > 3:248581/1‑141 (MQ=255) aaGGTGTCAATAATTTGTTTACTATACTTTGAGCGGCACTAGGGTTTAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAg > 1:1062792/1‑141 (MQ=255) aaGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAg > 1:806391/1‑141 (MQ=255) | ACACAGATCGTGATAACTTCTTAACTGCAGAAGAAGCTAAAGAATATGGCTTAATTGATGAAGTGATGGTACCTGAAACAAAATAATTCAAAGTAAAGAGTAGACTAAGCTGTCTGCTCTTTTTGTATGAGTAAACCAAGGTGTCAATAATTTGTTTACTATACTTTGAGCGGAAATATGATTGAATGAAGCTAGTTGAACCGTAACTATATGAAATGTTCCCTTCAAAGTAGACATTGAAAGGAACATTTCAATCCTTTGTTTGTAAGTCGCTCTAG > USA300TCH1516_ALE/852908‑853185 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |