Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 803,528 C→T 5.3% intergenic (‑228/+42) USA300HOU_RS03920 ← / ← dtpT Putative lipid kinase/Di‑/tripeptide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE803,5280CT 5.3% 470.8 / 10.6 151intergenic (‑228/+42)USA300HOU_RS03920/dtpTPutative lipid kinase/Di‑/tripeptide transporter
Reads supporting (aligned to +/- strand):  ref base C (57/86);  new base T (2/6);  total (59/92)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.25e-01

Only 100 of 155 total aligned reads displayed.

AAAATGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATT  >  USA300TCH1516_ALE/803388‑803668
                                                                                                                                            |                                                                                                                                            
aaaaTGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGc                                                                                                                                              >  2:1016816/1‑141 (MQ=255)
aaaaTGGTAATAAATGAAATTGATTAATATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTTATTAATAGAAAAAAAAAAAAAAACAGTAAGc                                                                                                                                              <  2:157315/141‑1 (MQ=255)
    tGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCAtt                                                                                                                                          <  2:634015/141‑1 (MQ=255)
      gTAATAACTGACATTGATGTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAACGAGCATATTAAGCTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTa                                                                                                                                        <  1:674957/141‑1 (MQ=255)
      gTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTa                                                                                                                                        <  2:206474/141‑1 (MQ=255)
      gTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTa                                                                                                                                        >  1:22835/1‑141 (MQ=255)
       taataaATGAAATTGATTTCTATAGATTGGTACGAAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATAATGATATCTTGAATTTAATTTATAAAAACAAATAAAAAAACAGTAAGCCATTTaaa                                                                                                                                      <  2:101726/141‑1 (MQ=255)
          taaATGAAATTGATTTATATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATg                                                                                                                                    <  2:229738/141‑1 (MQ=255)
            aaTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATATATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGAc                                                                                                                                  <  2:1058144/141‑1 (MQ=255)
            aaTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTAGAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGAc                                                                                                                                  <  1:853700/141‑1 (MQ=255)
             aTGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACt                                                                                                                                 <  1:305066/141‑1 (MQ=255)
              tGAAATTGATTTCTATAGAGTGGAACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACtt                                                                                                                                <  2:959757/141‑1 (MQ=255)
               gagaTTGATTTCTATAGAGAGGGAGGAGATAATATAGTTATTGGTGTCTATAATGAGCATATTAAATTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATCAAATTGCAGTAAGCCATTTAAATGACTTa                                                                                                                               <  2:52627/138‑1 (MQ=255)
                aaaTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTAc                                                                                                                              >  1:627851/1‑141 (MQ=255)
                aaaTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTAc                                                                                                                              <  2:625082/141‑1 (MQ=255)
                  aTTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTg                                                                                                                            >  2:791328/1‑141 (MQ=255)
                   ttGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTACTGt                                                                                                                           <  1:31092/141‑1 (MQ=255)
                   ttGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGTCATTTAAATGGCTTACTGt                                                                                                                           <  1:160139/141‑1 (MQ=255)
                   ttGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGt                                                                                                                           <  1:667848/141‑1 (MQ=255)
                      atgtCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAACGAGCATATTAAGCTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtttt                                                                                                                        <  4:114089/138‑1 (MQ=255)
                      aTTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtttt                                                                                                                        <  1:45500/141‑1 (MQ=255)
                       tttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttttt                                                                                                                       <  3:310383/141‑1 (MQ=255)
                       tttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttttt                                                                                                                       <  1:103497/141‑1 (MQ=255)
                       tttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGttttt                                                                                                                       <  1:103496/141‑1 (MQ=255)
                        ttCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGtttttt                                                                                                                      <  4:48174/141‑1 (MQ=255)
                         tCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTg                                                                                                                     >  1:445372/1‑141 (MQ=255)
                            ataGAGTGAGACTAGAAAATATAGTTATAGCATTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAAGTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAAt                                                                                                                  <  3:168512/141‑1 (MQ=255)
                               gagTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAg                                                                                                               <  2:68728/141‑1 (MQ=255)
                                agTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAgg                                                                                                              >  3:457171/1‑141 (MQ=255)
                                   gggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCca                                                                                                           <  1:453135/141‑1 (MQ=255)
                                    ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                          >  1:505835/1‑141 (MQ=255)
                                    ggACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaa                                                                                                          >  3:182260/1‑141 (MQ=255)
                                     gACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCcaac                                                                                                         <  3:135579/141‑1 (MQ=255)
                                      aCGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCAATTTAAATGACTTACTGTTTTTTGAATTAGGCcaaca                                                                                                        <  2:189681/141‑1 (MQ=255)
                                           aaaaTATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAtt                                                                                                   >  4:281830/1‑141 (MQ=255)
                                              atatAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAAc                                                                                                >  1:744355/1‑141 (MQ=255)
                                              atatAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAAc                                                                                                >  1:836730/1‑141 (MQ=255)
                                                   gTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtata                                                                                           >  4:394819/1‑141 (MQ=255)
                                                   gTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtata                                                                                           <  1:951369/141‑1 (MQ=255)
                                                   gTTATAGCTGTCTATAATGAGCATATTAAGTTTTTAGTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGtata                                                                                           <  2:168734/141‑1 (MQ=255)
                                                      ataGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCt                                                                                        >  1:560173/1‑141 (MQ=255)
                                                       taGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtt                                                                                       <  1:1053422/141‑1 (MQ=255)
                                                       taGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtt                                                                                       <  4:192649/141‑1 (MQ=255)
                                                        aGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACATTAAGCCATTTAAATGTCTTACTGTTTTTTGAATTAGGCCAACAACATTAACTAATACCttt                                                                                      >  2:318608/1‑141 (MQ=255)
                                                         gCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTCATAGAAAAATAAACAAAAACCGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCtttg                                                                                     <  1:1028971/141‑2 (MQ=255)
                                                         gCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTc                                                                                     >  1:16208/1‑141 (MQ=255)
                                                          cTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCa                                                                                    <  1:736514/141‑1 (MQ=255)
                                                               tataATGATCATATTAACTTTTTATTTATACTGATATCTTGAATTTAATTTATAGAAACCTATAAAAAAACAGTAACCCATTTAAATGCCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATCCCTTTCATCGCt                                                                               <  4:192937/141‑1 (MQ=255)
                                                               tataATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATcaaa                                                                               <  1:558300/141‑4 (MQ=255)
                                                                   aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtga                                                                           <  1:569004/141‑1 (MQ=255)
                                                                   aTGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTAATcatcaaag                                                                           <  2:236766/141‑8 (MQ=255)
                                                                    tGAGCATAGTAAGTTTTTATTGATACTGATATCTTGACTTTAATTAATAGAAAGCTATAAAAAAACAGTCAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtgat                                                                          <  2:562836/141‑1 (MQ=255)
                                                                     gAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTtgatg                                                                         <  2:917539/141‑1 (MQ=255)
                                                                        cATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGAtt                                                                      >  1:33812/1‑141 (MQ=255)
                                                                         atatTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTa                                                                     >  2:1061718/1‑141 (MQ=255)
                                                                           atTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTaaa                                                                   >  2:438854/1‑141 (MQ=255)
                                                                           atTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTaaa                                                                   >  3:249648/1‑141 (MQ=255)
                                                                            ttAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAg                                                                  <  2:162221/141‑1 (MQ=255)
                                                                             tAAGTTGTTATTTAGACTGATATCGTGAATTTAATTAATAGATACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATAGTAACGTATACCTTTCATCGCTTTGATGATTAAAgg                                                                 <  2:1039301/141‑1 (MQ=255)
                                                                               aGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTg                                                               <  1:323840/141‑1 (MQ=255)
                                                                               aGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTg                                                               >  2:86734/1‑141 (MQ=255)
                                                                                gcttttATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTga                                                              >  2:120290/3‑141 (MQ=255)
                                                                                   tttatttaTACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGAt                                                           <  3:410360/141‑1 (MQ=255)
                                                                                       tttatACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCt                                                       <  2:717704/141‑1 (MQ=255)
                                                                                          ataCTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGTCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAAt                                                    <  4:219955/141‑1 (MQ=255)
                                                                                            aCTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGAATGCTAATAc                                                  <  2:707605/141‑1 (MQ=255)
                                                                                             cTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACa                                                 <  3:292602/141‑1 (MQ=255)
                                                                                              tGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACaa                                                <  2:334195/141‑1 (MQ=255)
                                                                                              tGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACaa                                                <  1:806172/141‑1 (MQ=255)
                                                                                                atatCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAAtt                                              <  3:199924/141‑1 (MQ=255)
                                                                                                  atCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAAttgt                                            >  4:152517/1‑141 (MQ=255)
                                                                                                      tGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                        <  1:120128/141‑1 (MQ=255)
                                                                                                      tGAATCTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTa                                        <  1:110131/141‑1 (MQ=255)
                                                                                                       gAATTTAATTAATAGGAACCTATAAAAAAACAGTAAGCCATTTAGATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTaa                                       <  2:17221/141‑1 (MQ=255)
                                                                                                       gAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTaa                                       <  4:323620/141‑1 (MQ=255)
                                                                                                        aaTTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAAc                                      <  2:419618/141‑1 (MQ=255)
                                                                                                              attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACaataat                                >  1:276384/1‑141 (MQ=255)
                                                                                                              attaatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGGTTTTTGAATGAGGCCAACAAGATGAACGTAGACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTTTAACaataat                                >  1:488366/1‑141 (MQ=255)
                                                                                                                taatAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTATTGTTACAACAATTg                              <  3:39811/141‑1 (MQ=255)
                                                                                                                  atAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGca                            <  4:291043/141‑1 (MQ=255)
                                                                                                                   tAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaa                           <  1:1030250/141‑1 (MQ=255)
                                                                                                                     gAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGcaaca                         <  1:993869/141‑1 (MQ=255)
                                                                                                                             taAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAg                 <  2:656040/141‑1 (MQ=255)
                                                                                                                             taAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAg                 >  1:917248/1‑141 (MQ=255)
                                                                                                                               aaaaaaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGa               >  2:775826/1‑141 (MQ=255)
                                                                                                                               aaaaaaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGa               >  1:299195/1‑141 (MQ=255)
                                                                                                                               aaaaaaCAGTAAGCCATTTAAATGACTTACTGTCTTTAGAATTAGGCCAACAATATTAACGTATACCTTTCATCGATATGATGATAAAAGGTGAGATTGCCAATACAATTGTTGTAACTATAATTGCAACAACCCCTACGa               >  2:951850/1‑141 (MQ=255)
                                                                                                                                 aaaaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGaaa             >  4:260656/1‑141 (MQ=255)
                                                                                                                                   aaCAGTAATCCAGTTAAATGACTTAATGTTTGTGGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt           <  2:111718/141‑1 (MQ=255)
                                                                                                                                   aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCCACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt           <  3:394819/141‑1 (MQ=255)
                                                                                                                                   aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt           <  2:431886/141‑1 (MQ=255)
                                                                                                                                   aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt           <  2:178528/141‑1 (MQ=255)
                                                                                                                                   aaCAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAAt           <  1:995022/141‑1 (MQ=255)
                                                                                                                                       gTAAGTCATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg       >  1:1050222/1‑141 (MQ=255)
                                                                                                                                       gTAAGTCATTTAAATGGCTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg       >  3:135921/1‑141 (MQ=255)
                                                                                                                                       gTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg       <  3:323233/141‑1 (MQ=255)
                                                                                                                                       gTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAg       >  2:748575/1‑141 (MQ=255)
                                                                                                                                         aaGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTa     >  2:409535/1‑141 (MQ=255)
                                                                                                                                          aGCCATTTAAATGACTTACTGTTTTTTGTATTAGGCCAACAAAATTAACGGATACCATTCATCACTTTGATGATTAAAGGTCAGCTTGCTAGTCCAATTATTGCAACAATAATTGCAACAACAACAACAAAAATAAAGTaa    >  2:712195/1‑141 (MQ=255)
                                                                                                                                           gCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATTCCTTTCATCGCTTTTTTTTTTTAATGTGAGATTGCTAATACAATCGTTGTAACAATCATTGCAACAACCCCTCGGAAAATACAGTatt   <  4:127036/141‑3 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAAATGGTAATAAATGAAATTGATTTCTATAGAGTGGGACGAGAAAATATAGTTATAGCTGTCTATAATGAGCATATTAAGTTTTTATTTATACTGATATCTTGAATTTAATTAATAGAAACCTATAAAAAAACAGTAAGCCATTTAAATGACTTACTGTTTTTTGAATTAGGCCAACAATATTAACGTATACCTTTCATCGCTTTGATGATTAAAGGTGAGATTGCTAATACAATTGTTGTAACAATAATTGCAACAACACCTAGGAAAATAAAGTAATT  >  USA300TCH1516_ALE/803388‑803668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: