BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	770,855	A→G	6.8%	intergenic (+41/‑209)	ybaK → / → glcR	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK/HTH‑type transcriptional repressor GlcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	770,855	0	A	G	6.8%	389.8 / 14.3	132	intergenic (+41/‑209)	ybaK/glcR	Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK/HTH‑type transcriptional repressor GlcR
Reads supporting (aligned to +/- strand): ref base A (78/45); new base G (2/7); total (80/52)
Fisher's exact test for biased strand distribution p-value = 2.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.04e-01

Only 100 of 133 total aligned reads displayed.

CAGTGTGAGTGGTGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTT  >  USA300TCH1516_ALE/770715‑770991

cAGTGTGAGTGGTGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGa                                                                                                                                          <  2:928554/141‑1 (MQ=255)

 aGTGTGAGTGGTGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGAc                                                                                                                                         >  1:1026902/1‑141 (MQ=255)

   tgtgAGTGGTGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCag                                                                                                                                       <  1:640761/141‑1 (MQ=255)

       aGTGGTGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGAt                                                                                                                                   <  1:598322/141‑1 (MQ=255)

        gtggtgGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATg                                                                                                                                  >  2:457274/1‑141 (MQ=255)

           gtggGCTTCGAACAATGCAAATCACAATAGCTGTTGAGTATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGaaa                                                                                                                               >  2:132683/1‑141 (MQ=255)

            tggGCTTCGAACAATGCAAATCTCAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAAt                                                                                                                              >  4:319694/1‑141 (MQ=255)

            tggGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAAt                                                                                                                              >  2:659875/1‑141 (MQ=255)

             gggCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAAtt                                                                                                                             >  3:154106/1‑141 (MQ=255)

               gcatCGAACAATGCAAATCACAATAGCTGTTGAGGATCTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGCTTAGGAGAGTGACAGAGAGTAAAtttt                                                                                                                           >  2:374100/4‑141 (MQ=255)

               gCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAAtttt                                                                                                                           >  2:439952/1‑141 (MQ=255)

                 ttCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTta                                                                                                                         <  1:715872/141‑1 (MQ=255)

                  tCGAACAATGCAAATCACAAGAGCGGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTtat                                                                                                                        >  2:767303/1‑141 (MQ=255)

                          tGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCa                                                                                                                <  2:185657/141‑1 (MQ=255)

                          tGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCa                                                                                                                >  2:981408/1‑141 (MQ=255)

                             aaaTCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAGGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCAttt                                                                                                             >  1:852763/1‑141 (MQ=255)

                             aaaTCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTAAtct                                                                                                             >  4:452011/1‑139 (MQ=255)

                                   caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAATTTCATCTCTgtca                                                                                                       <  1:513468/141‑4 (MQ=37)

                                   caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAATTTCATCTCTGTca                                                                                                       <  4:448427/141‑1 (MQ=37)

                                   caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCca                                                                                                       <  1:343746/141‑1 (MQ=255)

                                   caATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATCTCTGCca                                                                                                       >  2:50117/1‑141 (MQ=255)

                                    aaTAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCcac                                                                                                      >  2:565472/1‑141 (MQ=255)

                                     aTAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAATTTCATCTCTGTcaca                                                                                                     <  1:89941/141‑1 (MQ=25)

                                      tAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAATTTCATCTCTGTCacac                                                                                                    <  2:747753/141‑1 (MQ=25)

                                      tAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCacac                                                                                                    <  1:196588/141‑1 (MQ=255)

                                      tAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATACAAGTAGGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCacac                                                                                                    <  1:510434/141‑1 (MQ=255)

                                       aGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAAATGAATTTTATAAAAATTTCATCTCTGTCACACt                                                                                                   <  3:247372/141‑1 (MQ=25)

                                        gCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTc                                                                                                  >  1:1012512/1‑141 (MQ=255)

                                          tGTTGAGGATTTGATTACAATACCTAAAGGCATAATTGGAGCAGTTATTCATGAATGATTAATACCTACAAAAAGTATGGGGCAAAATTAGGGGTGTGACGTAGATGAAATTTTTATAAAATTCATTTCTGCCACTCTCCt                                                                                                <  4:4047/141‑1 (MQ=255)

                                             tGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCtttt                                                                                             <  2:320156/141‑1 (MQ=255)

                                             tGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCtttt                                                                                             <  1:421627/141‑1 (MQ=255)

                                               aGGATTTGATTACAATAACTAAAGGCCAAAATTTAACTATTTTCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGCGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTttg                                                                                           >  2:322590/1‑141 (MQ=255)

                                               aGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTttg                                                                                           >  3:258730/1‑141 (MQ=255)

                                                ggATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTttga                                                                                          <  1:106296/141‑1 (MQ=255)

                                                 gATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGCGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTttgat                                                                                         <  2:304992/141‑1 (MQ=255)

                                                     tGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGaa                                                                                     >  3:127292/1‑141 (MQ=255)

                                                     tGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGaa                                                                                     >  1:668122/1‑141 (MQ=255)

                                                          aCAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGc                                                                                >  1:55545/1‑141 (MQ=255)

                                                          aCAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGc                                                                                >  2:623384/1‑141 (MQ=255)

                                                          aCAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGc                                                                                <  2:656335/141‑1 (MQ=255)

                                                               aaCTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCAtttt                                                                           >  4:221989/1‑141 (MQ=255)

                                                                aCTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTa                                                                          >  2:82466/1‑141 (MQ=255)

                                                                 cTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTAc                                                                         >  2:295342/1‑141 (MQ=255)

                                                                  tAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACg                                                                        >  1:1074941/1‑141 (MQ=255)

                                                                    aaGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGAt                                                                      <  2:794501/141‑1 (MQ=255)

                                                                    aaGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGAt                                                                      >  2:1042360/1‑141 (MQ=255)

                                                                     aGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATc                                                                     >  3:25364/1‑141 (MQ=255)

                                                                      ggCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCa                                                                    >  2:361457/1‑141 (MQ=255)

                                                                          aaaTTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATaaa                                                                >  3:309633/1‑141 (MQ=255)

                                                                             ttGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAg                                                             <  3:180752/141‑1 (MQ=255)

                                                                              tGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGt                                                            <  3:107083/141‑1 (MQ=255)

                                                                              tGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGAAAGATTAGGAGTGTGGCAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGt                                                            <  4:114826/141‑1 (MQ=255)

                                                                               gtaGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTc                                                           <  1:596126/139‑1 (MQ=255)

                                                                                gAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCa                                                          >  4:13268/1‑141 (MQ=255)

                                                                                  gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                        >  2:550963/1‑141 (MQ=255)

                                                                                  gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                        >  1:878744/1‑141 (MQ=255)

                                                                                  gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                        >  2:95295/1‑141 (MQ=255)

                                                                                  gCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCAtt                                                        >  3:232201/1‑141 (MQ=255)

                                                                                     gTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATtata                                                     <  2:229356/141‑1 (MQ=255)

                                                                                       tATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAAt                                                   <  2:500683/141‑1 (MQ=255)

                                                                                         tCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTg                                                 >  3:138371/1‑141 (MQ=255)

                                                                                            attaataatTAATAAAAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCAGAAACAGTCATTATAATTGAGt                                              <  4:23277/138‑1 (MQ=255)

                                                                                             tgaatgaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGGCAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTa                                             >  3:51598/1‑141 (MQ=255)

                                                                                               aatgaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTAtt                                           >  1:375621/1‑141 (MQ=255)

                                                                                                 tgaTTAATAACAACAAAGAGTATGGGGCGAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTg                                         <  1:698575/141‑1 (MQ=255)

                                                                                                 tgaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTg                                         >  1:408867/1‑141 (MQ=255)

                                                                                                  gaTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGa                                        >  3:303324/1‑141 (MQ=255)

                                                                                                   aTTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGaa                                       >  1:1063425/1‑141 (MQ=255)

                                                                                                    ttAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAAc                                      <  1:659875/141‑1 (MQ=255)

                                                                                                    ttAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAAc                                      <  2:841748/141‑1 (MQ=255)

                                                                                                      aataaCAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACAt                                    >  4:309984/1‑141 (MQ=255)

                                                                                                       ataaCAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATa                                   >  1:914219/1‑141 (MQ=255)

                                                                                                         aacaacAAAAAGTATGGGACAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATaaa                                 <  2:806168/141‑1 (MQ=255)

                                                                                                            aacaaAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATaaaaag                              <  1:1031100/141‑2 (MQ=255)

                                                                                                            aacaaAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAAt                              >  1:90338/1‑141 (MQ=255)

                                                                                                              caaAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGt                            <  1:1042360/141‑1 (MQ=255)

                                                                                                               aaaaaGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTa                           >  3:69001/1‑141 (MQ=255)

                                                                                                                  aagaaaggggCAAGCTTAGGAGTGTGACAGAGACCAACATTTCATAAAACTCATATCCGCCACACTCCTTATAGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAATATTAGAAAAAAACAATGTAAta                        >  4:153322/7‑140 (MQ=255)

                                                                                                                   aGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAttt                       >  4:133190/1‑141 (MQ=255)

                                                                                                                    gTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAtttt                      >  1:983944/1‑141 (MQ=255)

                                                                                                                    gTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAtttt                      >  1:488047/1‑141 (MQ=255)

                                                                                                                    gTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAAtttt                      >  2:171733/1‑141 (MQ=255)

                                                                                                                     tATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTa                     >  2:398380/1‑141 (MQ=255)

                                                                                                                     tATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTa                     >  2:398458/1‑141 (MQ=255)

                                                                                                                     tATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTa                     >  2:465880/1‑141 (MQ=255)

                                                                                                                       tGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTAtt                   >  1:584683/1‑140 (MQ=255)

                                                                                                                        ggggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCGTTTTTTTTTTTTTTGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATct                  >  2:1023401/1‑140 (MQ=255)

                                                                                                                         gggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGt                 >  2:800086/1‑141 (MQ=255)

                                                                                                                          ggCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTACTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTc                <  4:130858/141‑1 (MQ=255)

                                                                                                                            cAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTcac              <  4:56919/141‑1 (MQ=255)

                                                                                                                              aGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACaa            >  2:650550/1‑141 (MQ=255)

                                                                                                                                aTTAGGAGTGTGACAGAGATGAAATTTTTATAAAGTTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTGTACGATCATAAACAGTCATTATGATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAAtt          <  2:965030/141‑1 (MQ=255)

                                                                                                                                   aGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTTTTTTTTTTTTTATTTTAAGATCATAAACAGTCATTATAATTGAGTATTTGAACATAACAATGTACTATTAACGTTTCAATTTGa       >  2:490981/1‑141 (MQ=255)

                                                                                                                                   aGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGa       <  2:803734/141‑1 (MQ=255)

                                                                                                                                   aGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGa       >  1:598211/1‑141 (MQ=255)

                                                                                                                                   aGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGa       >  1:303094/1‑141 (MQ=255)

                                                                                                                                    ggAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAg      >  3:368982/1‑141 (MQ=255)

                                                                                                                                     gAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAgt     >  2:1045307/1‑141 (MQ=255)

                                                                                                                                      aGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAgtg    >  2:309659/1‑141 (MQ=255)

                                                                                                                                        tgtgACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAATCTGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGtt  <  2:1022349/141‑1 (MQ=255)

CAGTGTGAGTGGTGGGCTTCGAACAATGCAAATCACAATAGCTGTTGAGGATTTGATTACAATAACTAAAGGCAAAATTGGAGCAGTTATCCATGAATGATTAATAACAACAAAAAGTATGGGGCAAGATTAGGAGTGTGACAGAGATGAAATTTTTATAAAATTCATTTCTGCCACACTCCTTTTTGATTGAATTAGCATTTTACGATCATAAACAGTCATTATAATTGAGTATTTGAACATAAAAATGTAATTTTATCGTCACAATTTGAGTGTT  >  USA300TCH1516_ALE/770715‑770991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`