| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 632,475 | C→T | 6.0% | L200L (CTC→CTT) | proP → | Proline/betaine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 632,475 | 0 | C | T | 6.0% | 410.2 / 11.0 | 132 | L200L (CTC→CTT) | proP | Proline/betaine transporter |
| Reads supporting (aligned to +/- strand): ref base C (71/53); new base T (2/6); total (73/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGATTTTATTACAAAGATA > USA300TCH1516_ALE/632335‑632614 | cgtcgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTc < 2:300476/141‑1 (MQ=255) gtcgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCg > 1:73399/1‑141 (MQ=255) cgtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGt < 2:1022231/141‑1 (MQ=255) gtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGtt < 1:895519/141‑1 (MQ=255) gtAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCAGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTTGGtt < 1:213106/141‑1 (MQ=255) gtAACTCATTAGGTAGTGGACTAGAAATTGGGACAGTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGtt > 2:36350/1‑141 (MQ=255) aaCTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTa > 2:670977/1‑141 (MQ=255) aCTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATGCTTACTCGGTTTAt > 2:269382/1‑141 (MQ=255) gTAGTGGACTAGAAATTGGGGCATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGat < 4:456758/141‑1 (MQ=255) tAGTGGACTAGAAATTGGGACATTATCATGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGatt < 2:8586/141‑1 (MQ=255) tAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGatt < 2:710832/141‑1 (MQ=255) aGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGatta > 2:299755/1‑141 (MQ=255) gTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGattat < 2:632029/141‑1 (MQ=255) tGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGNGAATCCCATTCTTACTCGGNTTATTCCTAGGattatt < 3:276931/141‑1 (MQ=255) tGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGattatt < 1:412272/141‑1 (MQ=255) tGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGATTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGattatt < 2:290468/141‑1 (MQ=255) ggACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTc > 1:667859/1‑141 (MQ=255) aCTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATAGCATCATTTGGTTAGAGAACCCCATTCTTACTCGGTTTATTCCTAGGATTATTCgg > 2:852037/1‑141 (MQ=255) tAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCt < 1:220122/141‑1 (MQ=255) aaaTTGGGGCATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCAGTCTTACTCGGTTTATTCCTAGGATTATTCGGCTtat < 2:794225/141‑1 (MQ=255) aTTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCAGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTTGGTTTATTCCTAGGATTATTCGGCTTatat < 2:643144/141‑1 (MQ=255) tGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATAttt < 1:401504/141‑1 (MQ=255) ggACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAc > 1:27564/1‑141 (MQ=255) ggACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAc > 4:228199/1‑141 (MQ=255) aCATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgt > 3:364388/1‑141 (MQ=255) cATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtc < 3:364694/141‑1 (MQ=255) cATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtc < 1:1021573/141‑1 (MQ=255) aTTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtcg < 2:391884/141‑1 (MQ=255) aTTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtcg < 2:705897/141‑1 (MQ=255) ttATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtcgt < 1:59512/141‑1 (MQ=255) ttATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTAcgtcgt < 1:399392/141‑1 (MQ=255) tCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAg < 2:883969/141‑1 (MQ=255) cAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGc > 1:532704/1‑141 (MQ=255) cAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGc < 4:123945/141‑1 (MQ=255) aGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCt > 2:762178/1‑141 (MQ=255) aGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCt > 1:636595/1‑141 (MQ=255) aGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCt > 1:456622/1‑141 (MQ=255) ggTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTg < 2:576885/141‑1 (MQ=255) ggTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTg > 1:281959/1‑141 (MQ=255) tACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGgaa < 4:278530/141‑1 (MQ=255) tAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAAt > 2:625065/1‑141 (MQ=255) tAGCTGCTTCAATTATGATTGCTGTATTAACATTCGTTTGTACAGATGAACAAATGGCATCATTTGGTTGGAGATTCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAAt > 3:178071/1‑141 (MQ=255) gcttctTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTTGTAAGCTGGAAGAATCa < 4:282255/141‑1 (MQ=255) ctTAAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAAAAAATAAAAAAATTTGATTGGAGAATCAAATTCTTACTCGGTTTATTCATAGAATTAATCGGCTTATATTTACGTCGTAAGCTGgaaaaaaaaaaaa < 4:333306/141‑8 (MQ=255) ttCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCACCATTTGGTTGGAGCATCCACTTCTAACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGt < 1:919465/141‑1 (MQ=255) aaTTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTaa > 2:438910/1‑120 (MQ=255) tATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCAGTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCGGTTTTCGa > 1:933934/1‑141 (MQ=255) tGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGaaa > 1:259634/1‑141 (MQ=255) tGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGaaa < 1:152357/141‑1 (MQ=255) tGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGaaa < 3:132364/141‑1 (MQ=255) gATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGaaaa < 1:123142/141‑1 (MQ=255) tGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAatga > 1:1045699/1‑141 (MQ=255) gCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAatgat > 2:295287/1‑141 (MQ=255) gCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAatgat > 1:654317/1‑141 (MQ=255) ttAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCa > 1:291416/1‑141 (MQ=255) tAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCaa > 3:62004/1‑141 (MQ=255) cATTCTTTTTAACAGTTGAACATCTGGCATCATTTGGTTGGAGAATCCCATTCTTACTTGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTTGAAGAATCACCAGTTTTCGAAAATGATGTTGCAacac < 1:662643/141‑1 (MQ=255) cATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAacac > 1:310206/1‑141 (MQ=255) ttttttAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAAcc < 1:808254/140‑1 (MQ=255) cTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAAcc < 1:296384/141‑1 (MQ=255) cTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAAcc < 1:87101/141‑1 (MQ=255) tttttAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCa > 4:330833/1‑141 (MQ=255) ttttAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAg < 1:893016/141‑1 (MQ=255) tttAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGa < 2:175645/141‑1 (MQ=255) tttAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGa > 3:332519/1‑141 (MQ=255) aCAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTTGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTTGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGaacga > 3:216340/1‑138 (MQ=255) aCAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGCTTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGCTGTTGCAACACAACCAGAAaga > 1:550491/1‑141 (MQ=255) aGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAgaga < 2:504518/141‑1 (MQ=255) aGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAgaga > 3:120314/1‑141 (MQ=255) gATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGAt > 1:543540/1‑141 (MQ=255) gATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGAt > 1:910061/1‑141 (MQ=255) aTGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATa > 1:186456/1‑141 (MQ=255) tGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATaa > 2:777682/1‑141 (MQ=255) gAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAAc < 2:369642/141‑1 (MQ=255) aCAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACAt > 1:954332/1‑141 (MQ=255) cAAAGGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACAtt < 2:428519/141‑1 (MQ=255) aaTGGCATCATTTGGTTGGAGAATCCCATTCTTACTTGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTTGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAACGAGACAACATTaa < 2:421293/141‑1 (MQ=255) aaTGGCATCATTTGGATGGAGAATCCCATTCTTACTCGGTTTATTACTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGAAACACAACAAGAAAGAGATAAAATTaa > 2:702495/1‑141 (MQ=255) aTGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAAc < 2:463202/141‑1 (MQ=255) ggCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACtt < 1:490818/141‑1 (MQ=255) aacatcatTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCATTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACtt > 4:262114/3‑141 (MQ=255) gCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACttt > 1:677894/1‑141 (MQ=255) gCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACttt > 1:465963/1‑141 (MQ=255) catcatTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACtttt > 1:823786/1‑141 (MQ=255) catcatTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACtttt > 4:231728/1‑141 (MQ=255) catTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTAc > 1:1027531/1‑141 (MQ=255) tttGGTTGGAGAATCCCATTCTTACTTGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTTGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAACGAGACAACATTAACTTTTTACaa < 3:457811/141‑1 (MQ=255) tttGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACaa > 1:72198/1‑141 (MQ=255) ggttggAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAatc > 1:1068636/1‑141 (MQ=255) gttggAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAatca < 4:91621/141‑1 (MQ=255) gttggAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAatca > 4:83157/1‑141 (MQ=255) gagaATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAg < 2:227267/141‑1 (MQ=255) agaATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGa > 3:230802/1‑141 (MQ=255) aTCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGAttt > 1:1021516/1‑141 (MQ=255) cccATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGATTtta > 4:329977/1‑141 (MQ=255) aTTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGATTttatta > 2:566996/1‑141 (MQ=255) tCTTACTTGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTTGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAACGAGACAACATTAACTTTTTACAAATCATCAGATTTTATTACa < 4:182432/141‑1 (MQ=255) tACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGATTTTATTACAAAg > 2:469163/1‑141 (MQ=255) aCTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGATTTTATTACAAAGa > 2:141141/1‑141 (MQ=255) tCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGATTTTATTACAAAGata > 1:731559/1‑141 (MQ=255) | CGTCGTAACTCATTAGGTAGTGGACTAGAAATTGGGACATTATCAGGTTACATAGCTGCTTCAATTATGATTGCTGTATTAACATTCTTTTTAACAGATGAACAAATGGCATCATTTGGTTGGAGAATCCCATTCTTACTCGGTTTATTCCTAGGATTATTCGGCTTATATTTACGTCGTAAGCTGGAAGAATCACCAGTTTTCGAAAATGATGTTGCAACACAACCAGAAAGAGATAACATTAACTTTTTACAAATCATCAGATTTTATTACAAAGATA > USA300TCH1516_ALE/632335‑632614 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |