Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,558 T→C 8.0% S1220S (AGT→AGC)  sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5580TC 8.0% 378.8 / 8.4 145S1220S (AGT→AGC) sdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (54/75);  new base C (8/5);  total (64/81)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

AGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAG  >  USA300TCH1516_ALE/618420‑618697
                                                                                                                                          |                                                                                                                                           
aGACTCAGATAGCGACTCAGATTCAGACTGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGAGATGCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGa                                                                                                                                           <  2:28995/141‑1 (MQ=255)
 gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGAc                                                                                                                                          <  1:326099/141‑1 (MQ=255)
   cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCCGACAGCGACTCAGAATCCGCCAGCGACTAAGACTCAGATAGTGACtc                                                                                                                                        >  1:864195/1‑141 (MQ=17)
   cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtc                                                                                                                                        <  2:746199/141‑1 (MQ=255)
     cAGATCGCGACACAGATTCAGACCGCGACGCAGATTCAGAAAGAGACTCAGACTCAGACAATGACGCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcag                                                                                                                                      <  4:223585/141‑1 (MQ=14)
      aGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcaga                                                                                                                                     >  1:519819/1‑141 (MQ=255)
         tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGTCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                                                                                                  <  1:483336/141‑1 (MQ=255)
         tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                                                                                                  >  3:299687/1‑141 (MQ=255)
         tAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                                                                                                  >  1:690434/1‑141 (MQ=255)
         tAGCGACGTAGATTCAGACAACGACTCCGATTCAGACAGAGACACAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattc                                                                                                                                  <  2:184080/141‑1 (MQ=17)
          agcgactcagattcagacagcgactcagattcagacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattca                                                                                                                                 <  2:241889/141‑1 (MQ=255)
           gcgactcagattcagacagcgactcagattcagacaGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattcag                                                                                                                                <  1:346197/141‑1 (MQ=255)
           gcgactcagattcagacagcgactcagattcagacaGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGactcagactcag                                                                                                                                >  2:325629/1‑141 (MQ=255)
               ctcagattcagacagcgactcagattcagacagcgaCTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAg                                                                                                                            >  1:447309/1‑141 (MQ=255)
                   gattcagacagtgattcagattcagacggcgacccaGACTCAGACGGTGAGTAAGACTCAGATAGCGACTCAGATTCAGACGGCGACTCAGACTCAGACTGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAc                                                                                                                        <  2:90517/141‑1 (MQ=17)
                     ttcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTAAGATAGTGACTTAGATTCAGATAGCGACTc                                                                                                                      >  1:833583/1‑141 (MQ=255)
                      tcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCa                                                                                                                     >  3:280409/1‑141 (MQ=255)
                      tcagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACGCAGACTCAGATAGTGACTCAGACTCAGATAGCggcaca                                                                                                                     >  2:502841/1‑136 (MQ=255)
                       cagacagcgactcagattcaaacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAg                                                                                                                    >  4:336992/1‑141 (MQ=255)
                        agacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGa                                                                                                                   <  2:988933/141‑1 (MQ=255)
                           cagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTc                                                                                                                >  1:574076/1‑141 (MQ=255)
                               gattcagactcagacagcgactcagaTTCAGACAGTGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                                                                                                            >  1:923375/4‑141 (MQ=1)
                                   cagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGTCTCAGATTCAGATAGCGACTCAGATTCGGACAGCg                                                                                                        <  1:948684/141‑1 (MQ=255)
                                    agactcagacagcgactcagaTTCAGACAGTGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGa                                                                                                       <  1:174937/137‑1 (MQ=11)
                                     gattcagacagtgattcagaCTCAGACAGTGATTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                                                                                                      >  3:74896/1‑141 (MQ=11)
                                     gattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                                                                                                      >  3:364280/1‑141 (MQ=255)
                                     gattcagacagcgactcagaCTCAGATAGTGATTCAGACGCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGAGAGCGACTCAGACTCAGACAGCGACTCCGACACAGATAGCGACTCAGACTCCGACAGCGag                                                                                                      >  2:955888/1‑140 (MQ=14)
                                     gattcagacagagattaagaCTAAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAt                                                                                                      <  2:956975/141‑1 (MQ=255)
                                      attcagacagagactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAtt                                                                                                     <  4:427375/141‑1 (MQ=255)
                                       ttcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGAATCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTc                                                                                                    >  4:31361/1‑141 (MQ=255)
                                         cagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGAtacgg                                                                                                  >  2:178909/1‑137 (MQ=14)
                                          agacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                 <  3:457203/141‑1 (MQ=255)
                                           gacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGAc                                                                                                <  2:155484/141‑1 (MQ=255)
                                              agcgactcagaCTCAGATAGTGATTCAGACCCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCa                                                                                             >  2:1054550/1‑141 (MQ=255)
                                                      agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                                                                     <  1:476730/141‑1 (MQ=255)
                                                      agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                                                                     <  3:437340/141‑1 (MQ=255)
                                                      agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGa                                                                                     >  1:4001/1‑141 (MQ=255)
                                                      agactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACACAGATAGCGa                                                                                     >  2:231138/1‑141 (MQ=255)
                                                       gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                    <  1:672749/141‑1 (MQ=255)
                                                         ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCCGATAGCGACGCGGACTCGGAAACCGATTCATATGCTGATAGCGACtc                                                                                  >  2:430144/1‑141 (MQ=14)
                                                         ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                                                                  >  1:643348/1‑141 (MQ=255)
                                                         ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                                                                  <  1:508017/141‑1 (MQ=255)
                                                           cagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACCGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcag                                                                                <  4:262341/141‑1 (MQ=255)
                                                            agaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACCGCGACTCAGACTCAGCCAGCGACTCAGACTCAGATAGTGAATCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                               <  3:130460/141‑1 (MQ=255)
                                                               tAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattc                                                                            <  2:739173/141‑1 (MQ=255)
                                                                 gTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATGCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcag                                                                          >  1:758265/1‑141 (MQ=255)
                                                                  tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                         <  3:79597/141‑1 (MQ=255)
                                                                  cgATTCAGCCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                         <  2:622141/140‑1 (MQ=255)
                                                                     ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                      <  1:755263/141‑1 (MQ=255)
                                                                       cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                    >  3:184436/1‑141 (MQ=255)
                                                                       cAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                    >  1:122267/1‑141 (MQ=255)
                                                                         gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                  <  1:493575/141‑1 (MQ=255)
                                                                         gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                  >  1:1027116/1‑141 (MQ=255)
                                                                           cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTc                                                                >  1:433072/1‑141 (MQ=255)
                                                                           cTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACCCAGATTCAGAGAGCGAGTCAGACACAGATAGCGACTCAGATTCAGAGAGCGATTc                                                                >  2:665396/1‑141 (MQ=14)
                                                                             cAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCgg                                                              >  1:403140/1‑141 (MQ=255)
                                                                             cAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCgg                                                              >  2:152002/1‑141 (MQ=255)
                                                                              aGATAGCGACTCAGATTCCGACAGCGACTCAGACTCAGCCAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGCTTCGGACAGCGATTCCGACTCAGATAGCGACTCAGATTCAGCTAGCGATTCGGa                                                             <  2:500389/141‑1 (MQ=14)
                                                                              aGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGAATCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACCCAGATTCAGATAGCGATTCGGa                                                             <  4:360173/141‑1 (MQ=255)
                                                                               taTAGCGACACAGATTCAGACAGTGACGCAGACTCAGACAGCGACGCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                            <  4:140528/140‑1 (MQ=255)
                                                                               gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                            <  2:385068/141‑1 (MQ=255)
                                                                                    cgactcagattcagacagcgactcagattcagacagCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                       <  3:399434/141‑1 (MQ=255)
                                                                                       cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                    <  3:326624/141‑1 (MQ=255)
                                                                                       cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                    >  3:265096/1‑141 (MQ=255)
                                                                                        tCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATTGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                   <  1:352798/141‑1 (MQ=255)
                                                                                          aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                 <  1:565019/141‑1 (MQ=255)
                                                                                          aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                 <  1:14814/141‑1 (MQ=255)
                                                                                          aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                 <  2:391757/141‑1 (MQ=255)
                                                                                           gATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGTGATTCAGAATCAGATAGCGACTCAGATTCGGACAGCGAATCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGAc                                                >  2:251454/1‑141 (MQ=11)
                                                                                           gATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGATAGCGAc                                                >  2:561958/1‑141 (MQ=255)
                                                                                             ttgagacagcgactcagacacagacagcgactcagacagAGATAGTGACTCAGATTCAGACAATCACTCAGACTAGGAAAGAGAATCAGACCAAGAGAGCGACTCAGACTCAGATAGAGATACAGACCCAGATAGCAAcac                                              >  4:81059/4‑139 (MQ=255)
                                                                                                   cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                        >  1:217488/1‑141 (MQ=255)
                                                                                                     gcgactcagattcagacagcgattcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                      <  4:74896/141‑1 (MQ=255)
                                                                                                     gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                      >  1:1033496/1‑141 (MQ=255)
                                                                                                     gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                      <  1:668869/141‑1 (MQ=255)
                                                                                                     gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                      <  2:1053140/141‑1 (MQ=255)
                                                                                                      cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                     <  1:592580/141‑1 (MQ=255)
                                                                                                      caactcagactcacacggcaactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                     <  2:589198/139‑1 (MQ=255)
                                                                                                       gactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcagat                                    <  1:978080/141‑1 (MQ=255)
                                                                                                       gactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcagat                                    <  1:75178/141‑1 (MQ=255)
                                                                                                           cagattcagacagagactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGATAGCGATTCAGACTCAGATAGCGAGTCAGATTCAGATAGAGAGTCAGAGTCTGATATAGACTCAGATTCCGACAggg                                >  4:143442/1‑139 (MQ=11)
                                                                                                            agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                               <  1:986128/141‑1 (MQ=255)
                                                                                                            agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                               <  2:530985/141‑1 (MQ=255)
                                                                                                             gactcagaccgcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                              <  3:207216/141‑1 (MQ=255)
                                                                                                                   gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc                        <  1:1048931/141‑1 (MQ=255)
                                                                                                                       gcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGAATCAGACTCaa                    >  2:34816/1‑140 (MQ=255)
                                                                                                                        cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCATATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                   <  1:295112/141‑1 (MQ=255)
                                                                                                                           ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                <  1:336381/141‑1 (MQ=255)
                                                                                                                            tcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGc               <  3:383667/141‑1 (MQ=255)
                                                                                                                             tcgactcagaTTGCGCCACAGAATCAGATTCCGATTCTGAATCCGACGGAGAGTCAGAATCAGATAGCGACTCAGATTCAGATAGCGATTCAGATTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg              <  2:853335/139‑1 (MQ=11)
                                                                                                                             cagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCg              >  2:1000214/1‑141 (MQ=255)
                                                                                                                               gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc            >  1:449225/1‑141 (MQ=255)
                                                                                                                               gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc            >  1:929905/1‑141 (MQ=255)
                                                                                                                                 ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc          <  1:152002/141‑1 (MQ=255)
                                                                                                                                 ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc          <  3:391663/141‑1 (MQ=255)
                                                                                                                                 ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACCCAGATAGCGACTc          >  2:423173/1‑141 (MQ=255)
                                                                                                                                 ctcagaTACTGACTCAGATTCAGACAGCGACTCAGATTCAGACAGTGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc          <  2:356082/141‑1 (MQ=255)
                                                                                                                                    agaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGa       >  1:248779/1‑141 (MQ=255)
                                                                                                                                     gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAt      >  3:50500/1‑141 (MQ=255)
                                                                                                                                         gTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAg  >  1:1059236/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAG  >  USA300TCH1516_ALE/618420‑618697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑