Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 613,998 G→A 5.1% S770S (TCG→TCA)  sdrC → Serine‑aspartate repeat‑containing protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE613,9980GA 5.1% 403.6 / 2.7 136S770S (TCG→TCA) sdrCSerine‑aspartate repeat‑containing protein C
Reads supporting (aligned to +/- strand):  ref base G (59/69);  new base A (5/3);  total (64/72)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 140 total aligned reads displayed.

TCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGA  >  USA300TCH1516_ALE/613858‑614138
                                                                                                                                            |                                                                                                                                            
tCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCg                                                                                                                                              <  2:763519/141‑1 (MQ=255)
   gACAGCGATTCAGACTCAGATATCGACTTAGATTCAGATAGCGACTCAGACTCAGACACCGATTCAGACAGCGATGCAGACTCAGATAGCGAATCAGATTCTGACAGCGACTCAAACTCAGACAGCGAGACAGACTCggct                                                                                                                                           >  3:190488/1‑139 (MQ=18)
   gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGAt                                                                                                                                           >  1:69052/1‑141 (MQ=255)
     cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAg                                                                                                                                         <  1:949997/141‑1 (MQ=255)
     cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAg                                                                                                                                         <  1:622813/141‑1 (MQ=255)
        cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGGCAGCGATTCAGACTCGGATAGCga                                                                                                                                      >  1:765087/1‑141 (MQ=255)
        cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCga                                                                                                                                      <  1:840615/141‑1 (MQ=255)
        cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCga                                                                                                                                      <  3:100630/141‑1 (MQ=255)
         gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgac                                                                                                                                     <  1:1011077/141‑1 (MQ=255)
             cAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcag                                                                                                                                 <  1:762451/141‑1 (MQ=255)
             cAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGAAAGCGATTCAGACAGGGACGCAGACTCAGATAGCGGTAGAGATTCAGACAGGGACTCAGACTCAGACAGCAATTCAGATTCGGATAGCgacagag                                                                                                                                 >  4:393992/1‑137 (MQ=18)
              aGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcaga                                                                                                                                <  2:185361/141‑1 (MQ=255)
               gACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcagac                                                                                                                               >  1:508039/1‑141 (MQ=255)
               gACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACACCGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCGGACAGCgactcagac                                                                                                                               >  2:579353/1‑141 (MQ=18)
                 cTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcagactc                                                                                                                             <  2:1078860/141‑1 (MQ=255)
                    agatagcgactcagattcagatagcgactcagattcAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGActcagactcaga                                                                                                                          <  2:65528/141‑1 (MQ=255)
                       tagcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAg                                                                                                                       <  1:955603/141‑1 (MQ=255)
                       tagcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAg                                                                                                                       >  3:228958/1‑141 (MQ=255)
                         gcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGAGACTGAGACTCAGATAGCGATTCAGATTCAGACGGCGACTCAGACTCGGATGGCGATTGAGAAGCGGATAGCGACACAGACTCAGATAGCg                                                                                                                     >  2:944327/1‑141 (MQ=18)
                          cgcctcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACACAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGa                                                                                                                    <  1:225967/138‑1 (MQ=255)
                          cgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGa                                                                                                                    <  1:147422/141‑1 (MQ=255)
                          cgactcagagtcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGa                                                                                                                    <  1:819685/141‑1 (MQ=255)
                           gactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGAGAGCGATTGAGACTCAGATAGCGACTCAGACTCAGATAGCGAc                                                                                                                   >  2:1086395/1‑141 (MQ=255)
                           gactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGAc                                                                                                                   <  2:459196/141‑1 (MQ=255)
                           gactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGAc                                                                                                                   >  1:274858/1‑141 (MQ=255)
                           gactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGAc                                                                                                                   <  3:38021/141‑1 (MQ=255)
                             ctcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTc                                                                                                                 >  1:806434/1‑141 (MQ=255)
                               cggattcagatagcggctcagattcagaCAGAGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAg                                                                                                               <  4:355669/139‑1 (MQ=255)
                               cagattcagatagcgcctcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAg                                                                                                               <  1:59492/141‑1 (MQ=255)
                               cagattcagatagcgactcagattcagaCAGCGATTCAGACTCAGACTCAGACTCAGATACCGATCCAGATTCAGACACCGACCCAGACTCAGACAGAGACTCAGACTCGGAGCCGGACTCCGACGCAGCTAGCGatcccg                                                                                                               >  2:204587/1‑136 (MQ=17)
                               cagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAg                                                                                                               <  1:793382/141‑1 (MQ=255)
                                   ttcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCATATTc                                                                                                           >  1:1042431/1‑141 (MQ=255)
                                    tcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCg                                                                                                          <  1:735211/141‑1 (MQ=255)
                                     cagatagtgactcagattcagaTAGCTATTCGGCGAGCGACTCAGACTCAGATAGCGATTCAGATTCATACAGCGACTCAGACTCAGACAGCGATTCAGATTCGGATAGCGATTCAGACTCAGATGGCGGCTCAGATTCgg                                                                                                         <  4:204648/141‑1 (MQ=18)
                                      agatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                                                                                                        <  3:219504/141‑1 (MQ=255)
                                       gatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAt                                                                                                       <  2:516578/141‑1 (MQ=255)
                                         tagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAg                                                                                                     <  1:531136/141‑1 (MQ=255)
                                         tagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAg                                                                                                     >  2:162073/1‑141 (MQ=255)
                                            cgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGCTTAAGAGTCGGCTAGCGACTCAGCGTCAGATAGCACCTCAGATTCTGATACCga                                                                                                  >  3:198990/1‑141 (MQ=18)
                                             ttctcatattgagaAATCGATTATAACAGAGATGCAGACTCGGATAGCGATTCAGATTCAGACATCAACTCAGACTCAGACAGCGATTCAGCCTCGGATAGCGACTCAGAGTCAGATAGCGACTCAGATTCGGATAGCgac                                                                                                 <  2:322728/139‑1 (MQ=14)
                                             gactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATGGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgac                                                                                                 <  2:342025/141‑1 (MQ=255)
                                             gactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGAGACTCAGATTCGGATAGCgac                                                                                                 <  3:238899/141‑1 (MQ=255)
                                             gactcagattcagaCAGCGATTCAGACAGCGACACAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGAGAGCGATTCGGACTCGGATAGCGACTCAGACTCAGATAGCGACACAGACTCAGATAGCgac                                                                                                 >  2:181920/1‑141 (MQ=18)
                                                tcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTGAGACTCGGAGAGCGACTCAGACTCAGAGAGCGACTCAGACTCAGATAGCgactca                                                                                              >  2:778332/1‑141 (MQ=17)
                                                     gtcagacagggattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgactcagactc                                                                                         <  2:1060882/140‑1 (MQ=255)
                                                      tcagacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCgactcagactca                                                                                        >  4:433555/1‑141 (MQ=255)
                                                      acagacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgactcagactca                                                                                        <  2:1086571/140‑1 (MQ=255)
                                                       cagatagagattcagacagcgaCGCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGactcagactcag                                                                                       <  4:360385/141‑1 (MQ=255)
                                                       cagacagcgattcagacagcgaCTCAGACTCAGACAGAGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGactcagactcag                                                                                       <  2:187298/141‑1 (MQ=255)
                                                             gcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCg                                                                                 >  2:737040/1‑141 (MQ=255)
                                                             gcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCg                                                                                 <  2:62105/141‑1 (MQ=255)
                                                               gaatcagatagcgaCTCAGAATCAGATAGCGAGTCAGATTCAGACAGAGACTCAGACTCAGACAGTGATTCAGACTCAGATAGCGATTCAGACTCAGATAGCGATTCAGATTCGGATAGCGACTCAGACTCAGATAGCgat                                                                               <  2:492649/138‑1 (MQ=11)
                                                                    agacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGTCTCAGACAGCgattcaga                                                                          <  3:216028/141‑1 (MQ=11)
                                                                     gacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCgattcagac                                                                         >  3:245646/1‑140 (MQ=11)
                                                                     gacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGCTTCAGACTCGGAGAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGACTCAGACTCAGATAGCgattcagat                                                                         >  3:457151/1‑141 (MQ=255)
                                                                     gacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattgagac                                                                         >  2:884320/1‑140 (MQ=255)
                                                                       cagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattcagattc                                                                       >  1:459618/1‑141 (MQ=255)
                                                                         gcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGattcagattcag                                                                     >  4:105270/1‑141 (MQ=255)
                                                                          cgaCTCAGATTCAGATAGCGATTCAGATTCTGACAGCGTTTCAGACTCAGACAGCGATTCAGACTCGGATAGCGATTCAGATTCAGATAGCGACTCATATTTGGATAGCGATGCAGATTCAGATTAGGAttcataatcaga                                                                    >  2:549146/1‑141 (MQ=14)
                                                                          cgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGAttcagattcaga                                                                    <  2:596798/141‑1 (MQ=255)
                                                                             ctcagactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                                                 >  1:106052/1‑141 (MQ=255)
                                                                              tcagactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAgg                                                                >  2:299020/1‑140 (MQ=255)
                                                                                agactcagaTAGCGATTCAGAATCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGa                                                              <  2:1043116/141‑1 (MQ=255)
                                                                                 gactcaggTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGACTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGAt                                                             >  3:352457/1‑141 (MQ=255)
                                                                                    tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATATCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCg                                                          >  1:703019/1‑141 (MQ=255)
                                                                                    tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGAGAACGACTcc                                                          >  2:410496/1‑140 (MQ=255)
                                                                                    tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAtaca                                                          >  2:13486/1‑138 (MQ=255)
                                                                                       gaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGAc                                                       >  1:105116/1‑141 (MQ=255)
                                                                                           gCGATTCAGATGCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAg                                                   <  2:940704/141‑1 (MQ=255)
                                                                                               ttcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAg                                               <  3:122270/141‑1 (MQ=255)
                                                                                                     ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                         <  3:53743/141‑1 (MQ=255)
                                                                                                     ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                         <  3:225464/141‑1 (MQ=255)
                                                                                                     ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGATTCAGACTCAGACAGAgattc                                         >  2:81181/1‑141 (MQ=255)
                                                                                                     ttcagaCACCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                         <  2:69052/141‑1 (MQ=255)
                                                                                                      tcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattca                                        >  1:245543/1‑141 (MQ=255)
                                                                                                         gaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagat                                     >  1:21833/1‑141 (MQ=255)
                                                                                                           cAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                                   >  3:258053/1‑141 (MQ=255)
                                                                                                             gCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGattcagnttcag                                 >  1:305175/1‑141 (MQ=255)
                                                                                                             gCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGattcagattcag                                 >  2:520536/1‑141 (MQ=14)
                                                                                                              cGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAttcagattcaga                                <  1:594627/141‑1 (MQ=255)
                                                                                                                 ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTc                             >  1:357593/1‑141 (MQ=255)
                                                                                                                  tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCa                            <  4:217394/141‑1 (MQ=255)
                                                                                                                    agactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGa                          <  1:533264/141‑1 (MQ=255)
                                                                                                                    agactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTCATTCAGCTTCAGACTCAGa                          <  2:222884/141‑1 (MQ=255)
                                                                                                                     gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGAt                         <  1:199903/141‑1 (MQ=255)
                                                                                                                     gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACTCAGAt                         >  2:937328/1‑141 (MQ=255)
                                                                                                                     gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACACAGAt                         >  2:685980/1‑141 (MQ=255)
                                                                                                                        tcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGc                      <  1:192038/141‑1 (MQ=255)
                                                                                                                           gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                   <  1:405584/141‑1 (MQ=255)
                                                                                                                           gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                   <  4:354458/141‑1 (MQ=255)
                                                                                                                           gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTCAGACACAGATACCGAc                   >  2:96932/1‑141 (MQ=255)
                                                                                                                               gCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAg               <  1:862036/141‑1 (MQ=255)
                                                                                                                                cGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATCGCGCTTCGGACTCAGACAGTGATTCAGATTCAGACGCAGCTAGCGACTCAGa              >  1:980228/1‑141 (MQ=255)
                                                                                                                                cGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGa              <  2:995693/141‑1 (MQ=255)
                                                                                                                                     cAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCga                                                                                                  >  1:367888/1‑52 (MQ=255)
                                                                                                                                      aGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGa        <  3:314051/141‑1 (MQ=255)
                                                                                                                                       gACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGAc       >  1:65276/1‑141 (MQ=255)
                                                                                                                                         cTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAg     >  1:640141/1‑141 (MQ=255)
                                                                                                                                          tCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGc    <  3:393489/141‑1 (MQ=255)
                                                                                                                                            ggATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGa  <  1:189155/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGA  >  USA300TCH1516_ALE/613858‑614138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑