Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 475,163 C→T 5.5% D159D (GAC→GAT yciC_1 → Putative metal chaperone YciC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE475,1630CT 5.5% 471.5 / 10.4 146D159D (GAC→GATyciC_1Putative metal chaperone YciC
Reads supporting (aligned to +/- strand):  ref base C (72/65);  new base T (3/5);  total (75/71)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 147 total aligned reads displayed.

TCATATATTGATGATGAACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGCAACCGA  >  USA300TCH1516_ALE/475023‑475302
                                                                                                                                            |                                                                                                                                           
 cATATATTGATGATGAACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACg                                                                                                                                            <  3:37915/141‑1 (MQ=255)
     tatTGATGATGAACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAAc                                                                                                                                        >  1:985041/1‑141 (MQ=255)
     tatTGATGATGAACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAAc                                                                                                                                        <  3:132522/141‑1 (MQ=255)
         gatgatgaACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGAt                                                                                                                                    >  1:732276/1‑141 (MQ=255)
         gatgatgaACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGAt                                                                                                                                    >  4:283006/1‑141 (MQ=255)
         gatgatgaACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGAt                                                                                                                                    <  4:191479/141‑1 (MQ=255)
           tgatgaACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGa                                                                                                                                  >  1:202814/1‑141 (MQ=255)
           tgatgaACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGa                                                                                                                                  >  4:218196/1‑141 (MQ=255)
               gaACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGt                                                                                                                              >  1:1006240/1‑141 (MQ=255)
                   ttGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAATAGATGAGCGTTCGa                                                                                                                          >  1:703947/1‑141 (MQ=255)
                   ttGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGa                                                                                                                          <  3:123679/141‑1 (MQ=255)
                    tGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACACATGAGCGTTCGAt                                                                                                                         >  4:380746/1‑141 (MQ=255)
                     ggCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGAtt                                                                                                                        >  3:235322/1‑141 (MQ=255)
                      gCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTg                                                                                                                       <  1:1026593/141‑1 (MQ=255)
                      gCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCCTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGGGTTGACGAAACAGATGAGCGTTCGATTg                                                                                                                       <  4:435527/141‑1 (MQ=255)
                        aTTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTTGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGATGAAACAGATGAACGTTCGATTGCt                                                                                                                     >  2:988133/1‑141 (MQ=255)
                         ttGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTg                                                                                                                    >  1:1033613/1‑141 (MQ=255)
                          tGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGa                                                                                                                   <  1:991351/141‑1 (MQ=255)
                           gATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTTGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGATGAAACAGATGAACGTTCGATTGCTGAt                                                                                                                  >  3:222538/1‑141 (MQ=255)
                            aTCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTTGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGATGAAACAGATGAACGTTCGATTGCTGAtt                                                                                                                 <  3:162488/141‑1 (MQ=255)
                                   aGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAAt                                                                                                          <  1:22844/141‑1 (MQ=255)
                                         ttGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCa                                                                                                    <  2:574988/141‑1 (MQ=255)
                                            ccGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGCTTTATTAATTGCCCACGt                                                                                                 <  4:63261/141‑1 (MQ=255)
                                            ccGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGt                                                                                                 <  3:409434/141‑1 (MQ=255)
                                            ccGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGt                                                                                                 >  2:380828/1‑141 (MQ=255)
                                              gTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTg                                                                                               <  2:287547/141‑1 (MQ=255)
                                              gTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTg                                                                                               >  1:813994/1‑141 (MQ=255)
                                               tttAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGa                                                                                              <  1:116315/141‑1 (MQ=255)
                                                ttAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGaa                                                                                             <  2:515248/141‑1 (MQ=255)
                                                 tAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAAt                                                                                            >  1:305863/1‑141 (MQ=255)
                                                   gATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAAttt                                                                                          <  2:326945/141‑1 (MQ=255)
                                                     tACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTCCGATTGCTGATTTATTAATTGACCAAGTTGAATTTtg                                                                                        <  1:786224/141‑1 (MQ=255)
                                                       cAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTtgtg                                                                                      >  2:465619/1‑141 (MQ=255)
                                                        aaTGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGa                                                                                     >  2:1005542/1‑141 (MQ=255)
                                                          tGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATg                                                                                   <  4:238868/141‑1 (MQ=255)
                                                             ttACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAGCTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTAt                                                                                <  3:387456/141‑1 (MQ=255)
                                                               aCAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTg                                                                              <  1:614750/141‑1 (MQ=255)
                                                                 aGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGat                                                                            >  3:167226/1‑141 (MQ=255)
                                                                   ttGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGatta                                                                          <  1:196033/141‑1 (MQ=255)
                                                                       ggATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATtaa                                                                      <  2:732276/141‑1 (MQ=255)
                                                                        gATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATtaat                                                                     <  1:172165/141‑1 (MQ=255)
                                                                        gATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATtaat                                                                     <  1:838074/141‑1 (MQ=255)
                                                                        gATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATtaat                                                                     <  2:428987/141‑1 (MQ=255)
                                                                          tGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATtaataa                                                                   >  1:51773/1‑141 (MQ=255)
                                                                          tGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATtaataa                                                                   >  3:324743/1‑141 (MQ=255)
                                                                          tGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGATCGATTGCTGATTTATTAATTGACAAAGGTGAATATTGTGATTTATTGATTATtaataa                                                                   >  2:503848/1‑141 (MQ=255)
                                                                             tAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAAt                                                                <  1:245549/141‑1 (MQ=255)
                                                                              aaCCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGt                                                                                  <  2:21929/122‑1 (MQ=255)
                                                                              aaCCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGt                                                                                  >  1:21929/1‑122 (MQ=255)
                                                                              aaCCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAAtt                                                               >  2:198448/1‑141 (MQ=255)
                                                                                ccGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGa                                                             >  1:877518/1‑141 (MQ=255)
                                                                                 cGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGAt                                                            >  2:90751/1‑141 (MQ=255)
                                                                                     tcatACATGACTTCAGCTCAGAAGATTTAGTGATGAATCGTGATAAAAGGGTTGAAGAAACAGGTGAGTGTTCGATTGCTGTTGTATTAATTGACCAAGTTGAATTTTGTAGGGTAGTGATTATTAATAAGATTGATGTaa                                                        <  2:78857/138‑1 (MQ=255)
                                                                                      cGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAAt                                                       >  1:537222/1‑141 (MQ=255)
                                                                                      cGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAAt                                                       >  3:99038/1‑141 (MQ=255)
                                                                                        tACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTa                                                     >  1:830117/1‑141 (MQ=255)
                                                                                          cATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAgg                                                   <  3:44388/141‑2 (MQ=255)
                                                                                              aCATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTgaag                                               <  1:214698/141‑1 (MQ=255)
                                                                                               cATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTgaaga                                              >  3:313896/1‑141 (MQ=255)
                                                                                               cATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTgaaga                                              <  3:296447/141‑1 (MQ=255)
                                                                                               cATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTgaaga                                              <  3:11571/141‑1 (MQ=255)
                                                                                                  cAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTgaagaaga                                           <  4:136682/141‑1 (MQ=255)
                                                                                                  cAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTgaagaaga                                           <  3:106731/141‑1 (MQ=255)
                                                                                                   aaCTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGATGAAACAGATGAACGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTgaagaagaa                                          <  2:1068703/141‑1 (MQ=255)
                                                                                                     cTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACt                                        <  2:996966/141‑1 (MQ=255)
                                                                                                      tCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTa                                       <  2:972965/141‑1 (MQ=255)
                                                                                                      tCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTa                                       >  1:423780/1‑141 (MQ=255)
                                                                                                       cAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAg                                      <  4:161939/141‑1 (MQ=255)
                                                                                                       cAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAg                                      <  2:47755/141‑1 (MQ=255)
                                                                                                        agaagaTTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGc                                     <  2:953987/141‑1 (MQ=255)
                                                                                                        agaagaTTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGc                                     >  3:37894/1‑141 (MQ=255)
                                                                                                        agaagaTTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGc                                     >  2:660222/1‑141 (MQ=255)
                                                                                                         gaagaTTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCg                                    >  1:1081537/1‑141 (MQ=255)
                                                                                                         gaagaTTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCg                                    >  1:406646/1‑141 (MQ=255)
                                                                                                          aagaTTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGa                                   <  3:370377/141‑1 (MQ=255)
                                                                                                               ttATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTa                              >  2:1015191/1‑141 (MQ=255)
                                                                                                                 aTTGATGGATCGTGATCAAAGCGTTGATGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGACTTAATAAGTGAAGAAGAACTAGCGAAGTTAGa                            >  1:573947/1‑141 (MQ=255)
                                                                                                                  ttGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGaa                           >  1:665290/1‑141 (MQ=255)
                                                                                                                   tGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGaaa                          <  2:580793/141‑1 (MQ=255)
                                                                                                                     aTGGATCGTGATCAAAGCGTTGATGAAACAGATGAACGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTTGCAAAGTTAGaaaaa                        <  1:988133/141‑1 (MQ=255)
                                                                                                                       ggATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAAt                      <  1:380828/141‑1 (MQ=255)
                                                                                                                        gATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATg                     >  4:72284/1‑141 (MQ=255)
                                                                                                                        gATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAAGTGATTTAATTAGTGTAGAAAATATAGCGAAGGTAGAAAAAATg                     >  1:855715/1‑141 (MQ=255)
                                                                                                                         aTCGTGATCAAAGCGTTGATGAAACAGATGAACGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTTGCAAAGTTAGAAAAAgtgt                    <  1:925697/141‑1 (MQ=255)
                                                                                                                          tCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGtt                   <  2:656789/141‑1 (MQ=255)
                                                                                                                            gTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTaa                 >  1:742853/1‑141 (MQ=255)
                                                                                                                               aTCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAgcg              >  3:147368/1‑141 (MQ=255)
                                                                                                                               aTCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAgcg              <  1:931003/141‑1 (MQ=255)
                                                                                                                                tCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAgcgc             <  1:1066132/141‑1 (MQ=255)
                                                                                                                                 cAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCa            >  4:280683/1‑141 (MQ=255)
                                                                                                                                  aaaGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCAt           <  3:230350/141‑1 (MQ=255)
                                                                                                                                  aaaGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCAt           >  1:455482/1‑141 (MQ=255)
                                                                                                                                   aaGCGTTGACGAAATAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCAtt          <  2:703947/141‑1 (MQ=255)
                                                                                                                                     gCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGc        <  1:794467/141‑1 (MQ=255)
                                                                                                                                     gCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGc        >  2:386558/1‑141 (MQ=255)
                                                                                                                                      cGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGCa       >  3:249183/1‑141 (MQ=255)
                                                                                                                                        ttGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGCAAc     <  1:293145/141‑1 (MQ=255)
                                                                                                                                           aCGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGCAACCGa  >  1:621253/1‑141 (MQ=255)
                                                                                                                                           aCGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGCAACCGa  >  2:9285/1‑141 (MQ=255)
                                                                                                                                           aCGAAACAGATGAGCGTTCGATTGCTGATATATTAATTGACCAAGTTGAATTTTGAGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATATTAAGCGCATTGCAACCGa  >  2:702094/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TCATATATTGATGATGAACTTGGCATTGATCTTACAGCGATTTGCCGTTTAGATACAATGGTTACAGTTGTGGATGCTAACCGCTTCGTACATGACATCAACTCAGAAGATTTATTGATGGATCGTGATCAAAGCGTTGACGAAACAGATGAGCGTTCGATTGCTGATTTATTAATTGACCAAGTTGAATTTTGTGATGTATTGATTATTAATAAAATTGATTTAATTAGTGAAGAAGAACTAGCGAAGTTAGAAAAAATGTTAAGCGCATTGCAACCGA  >  USA300TCH1516_ALE/475023‑475302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: