BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	473,952	T→C	5.2%	D83D (GAT→GAC)	USA300HOU_RS02255 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	473,952	0	T	C	5.2%	309.5 / 3.5	111	D83D (GAT→GAC)	USA300HOU_RS02255	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (55/49); new base C (2/4); total (58/53)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

ACAGTTATTGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGCTTCTTTT  >  USA300TCH1516_ALE/473812‑474091

acaGTTATTGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGAt                                                                                                                                             <  1:715063/141‑1 (MQ=255)

     tATTGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGt                                                                                                                                        >  1:1076950/1‑141 (MQ=255)

        tGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTaa                                                                                                                                     <  1:868323/141‑1 (MQ=255)

        tGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTaa                                                                                                                                     >  1:729732/1‑141 (MQ=255)

        tGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTaa                                                                                                                                     <  1:566904/141‑1 (MQ=255)

        tGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTaa                                                                                                                                     >  1:400779/1‑141 (MQ=255)

              tGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTGAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGc                                                                                                                               <  3:377746/141‑1 (MQ=255)

              tGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGc                                                                                                                               <  1:267925/141‑1 (MQ=255)

                aaCATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCa                                                                                                                             <  2:124970/141‑1 (MQ=255)

                    tAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTc                                                                                                                         <  1:879595/141‑1 (MQ=255)

                    tAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTc                                                                                                                         <  2:344073/141‑1 (MQ=255)

                     aaGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGGACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCt                                                                                                                        >  4:455340/1‑141 (MQ=255)

                      aGCATNAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGNGNANCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTg                                                                                                                       <  1:354950/141‑1 (MQ=255)

                      aGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTg                                                                                                                       <  3:80543/141‑1 (MQ=255)

                      aGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTg                                                                                                                       >  1:42966/1‑141 (MQ=255)

                      aGCACAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTg                                                                                                                       <  1:1043632/141‑1 (MQ=255)

                         aTAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTgaga                                                                                                                    <  3:133475/141‑1 (MQ=255)

                         aTAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTgaga                                                                                                                    <  1:523764/141‑1 (MQ=255)

                             aTACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGa                                                                                                                <  3:402873/141‑1 (MQ=255)

                             aTACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGa                                                                                                                >  4:405116/1‑141 (MQ=255)

                              tACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGAc                                                                                                               >  1:211815/1‑141 (MQ=255)

                               ataaaGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTGATTGAACAAATGGCGCAGCATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGACTGGGTTAAAAGTGCAATGTCTGAGATAGACa                                                                                                              <  2:420178/139‑1 (MQ=255)

                                 aaaGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGc                                                                                                            <  1:995857/141‑1 (MQ=255)

                                  aaGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCa                                                                                                           >  1:1043941/1‑141 (MQ=255)

                                    gATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCAtt                                                                                                         <  1:78152/141‑1 (MQ=255)

                                    gATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGAGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCAtt                                                                                                         <  2:573249/141‑1 (MQ=255)

                                       atTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAg                                                                                                      >  2:714796/1‑141 (MQ=255)

                                        ttGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAgg                                                                                                     <  1:591670/141‑1 (MQ=255)

                                         tGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGCGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGa                                                                                                    <  3:300430/141‑1 (MQ=255)

                                          gAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGAt                                                                                                   <  2:42299/141‑1 (MQ=255)

                                          gAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGAt                                                                                                   >  1:983322/1‑141 (MQ=255)

                                          gAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGAt                                                                                                   >  2:346272/1‑141 (MQ=255)

                                              aCGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGAcc                                                                                               <  1:1015380/141‑1 (MQ=255)

                                               cGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGACATGTATGAGATAGACAGCATTAAGGATGACCt                                                                                              <  4:246658/141‑1 (MQ=255)

                                                  aTTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGaa                                                                                           >  3:293977/1‑141 (MQ=255)

                                                   ttAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGaaa                                                                                          >  2:430284/1‑141 (MQ=255)

                                                    tAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGaaaa                                                                                         >  1:867766/1‑141 (MQ=255)

                                                     aaaaGGTGAAGAAAAAGTATTGATTGAACAAATGGCGCAGCATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGACTGGGTTAAAAGTGCAATGTCTGAGATAGACAGTATTAAAGATGATTTGaaaaa                                                                                        >  4:435195/1‑141 (MQ=255)

                                                     aaaaGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGAGTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACGTGaaaaa                                                                                        >  4:305343/1‑141 (MQ=255)

                                                      aaaGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGaaaaaa                                                                                       >  1:838336/1‑141 (MQ=255)

                                                       aaGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGaaaaaaa                                                                                      <  1:913607/141‑1 (MQ=255)

                                                        aGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAAt                                                                                     <  2:836312/141‑1 (MQ=255)

                                                         ggTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAAtt                                                                                    >  2:188720/1‑141 (MQ=255)

                                                          gTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTa                                                                                   <  1:906372/141‑1 (MQ=255)

                                                            gaagaaAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGACGAAAAAGGAGCTCGGGTTCAGCATAACCAGCCTGGAATAGAAAGCATTAAGGCTGACCTGAAAAAAATTAAt                                                                                 >  1:431877/1‑141 (MQ=255)

                                                              agaaAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAg                                                                               <  2:355692/141‑1 (MQ=255)

                                                               gaaAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGc                                                                              >  1:407554/1‑141 (MQ=255)

                                                                  aaaGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTaa                                                                           <  1:986997/141‑1 (MQ=255)

                                                                   aaGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTaaa                                                                          >  4:113595/1‑141 (MQ=255)

                                                                     gTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACt                                                                        >  3:135497/1‑141 (MQ=255)

                                                                        ttAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACa                                                                     >  4:218249/1‑141 (MQ=255)

                                                                           aTTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACatat                                                                  >  1:418767/1‑141 (MQ=255)

                                                                             tGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCa                                                                <  1:436770/141‑1 (MQ=255)

                                                                             tGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCa                                                                <  4:293977/141‑1 (MQ=255)

                                                                              gAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCAt                                                               <  2:89533/141‑1 (MQ=255)

                                                                              gAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCACAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCAt                                                               >  1:551339/1‑141 (MQ=255)

                                                                                 cAAATGGTGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCAtact                                                            >  2:308278/1‑141 (MQ=255)

                                                                                 cAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCAtact                                                            <  3:332768/141‑1 (MQ=255)

                                                                                   aaTGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCAtactac                                                          <  1:629520/141‑1 (MQ=255)

                                                                                     tGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGTTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATAcaacta                                                        >  1:338609/1‑141 (MQ=255)

                                                                                     tGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATActacta                                                        >  2:531075/1‑141 (MQ=255)

                                                                                     tGGCGCAACATTGCGAAGCTTTTAAAGCTAATCTTAAAGCTGCAGCTCAAGGAGTTTGGGTTAAAAGTGTCATGTCTTAGATAGGCAGCATTAACGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATActacta                                                        >  4:271364/1‑141 (MQ=255)

                                                                                      ggCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACtaa                                                       <  2:870076/141‑1 (MQ=255)

                                                                                      ggCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACtaa                                                       >  2:282428/1‑141 (MQ=255)

                                                                                          cAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACtaaataa                                                   <  3:270721/141‑1 (MQ=255)

                                                                                                tGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGAc                                             <  1:947710/141‑1 (MQ=255)

                                                                                                 gCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGAcc                                            <  3:354810/141‑1 (MQ=255)

                                                                                                  cGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCCTGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCa                                           >  1:413005/1‑141 (MQ=255)

                                                                                                  cGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCa                                           >  2:257560/1‑141 (MQ=255)

                                                                                                  cGAAGCTTGTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATGGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACGACAGATGATACTACTAAATAAAGCGACCa                                           >  2:919543/1‑141 (MQ=255)

                                                                                                  cGAAGCTTGTAAAGACAATTTTAAAGGTGCAGCTGAATGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAACCTACATATCATACTACTAAATCAAGCGACCa                                           <  4:327883/141‑1 (MQ=255)

                                                                                                    aaGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAAt                                         >  1:926937/1‑141 (MQ=255)

                                                                                                    aaGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAAt                                         >  4:347180/1‑141 (MQ=255)

                                                                                                      gCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATTACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGt                                       <  4:318671/141‑1 (MQ=255)

                                                                                                         tttAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCa                                    <  1:365927/141‑1 (MQ=255)

                                                                                                          ttAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGACTGGGTTAAAAGTGCAATGTCTGAGATAGACAGTATTAAAGATGATTTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAg                                   <  4:290770/141‑1 (MQ=255)

                                                                                                              aGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGtata                               <  2:510542/141‑1 (MQ=255)

                                                                                                              aGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGtata                               <  3:89582/141‑1 (MQ=255)

                                                                                                               gCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTatat                              <  1:667454/141‑1 (MQ=255)

                                                                                                                cTAATTTTAAAGGTGCAGCTCAAGGAGACTGGGTTAAAAATGCAATGTCTGAGATAGACAGTATTAAAGATGATTTGAAAAAAATTAATAGCTACACTACATATCATACTACTAACTAAAGCGACCAATGTTCAGTATAtt                             <  2:1039506/141‑1 (MQ=255)

                                                                                                                 tAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTc                            >  1:207927/1‑141 (MQ=255)

                                                                                                                  aaTTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTca                           >  2:672663/1‑141 (MQ=255)

                                                                                                                      ttAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAAc                       >  1:474291/1‑141 (MQ=255)

                                                                                                                         aaGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGa                    >  3:336905/1‑141 (MQ=255)

                                                                                                                         aaGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGa                    >  2:912712/1‑141 (MQ=255)

                                                                                                                         aaGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGa                    >  2:912711/1‑141 (MQ=255)

                                                                                                                           ggTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGaca                  >  2:602211/1‑141 (MQ=255)

                                                                                                                            gTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGacac                 >  2:810536/1‑141 (MQ=255)

                                                                                                                              gCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTa               >  2:858237/1‑141 (MQ=255)

                                                                                                                                 gCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGAAAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGc            <  4:387469/141‑1 (MQ=255)

                                                                                                                                  cTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGcc           >  2:717382/1‑141 (MQ=255)

                                                                                                                                    cAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGc         <  1:1059015/141‑1 (MQ=255)

                                                                                                                                     aaGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGct        >  1:113768/1‑141 (MQ=255)

                                                                                                                                      aGGAGACTGGGTTAAAAGTGCAATGTCTGAGACAGACAGTATTAAAGATGACTTGAAAAAACTTAACAGATAAACTACCCATCATACTACTAAATAAAGCGACCAATGTTCCGTATATTAACAACTGCCACTAcgccgcct       >  2:988684/1‑139 (MQ=255)

                                                                                                                                       ggAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGcttc      >  2:708146/1‑141 (MQ=255)

                                                                                                                                       ggAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGcttc      <  1:1019956/141‑1 (MQ=255)

                                                                                                                                        gagaTTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGcttct     <  2:1043941/141‑1 (MQ=255)

                                                                                                                                          gaTTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGCTTCttt   >  1:243318/1‑141 (MQ=255)

                                                                                                                                          gaTTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGCTTCttt   >  3:316252/1‑141 (MQ=255)

                                                                                                                                           aTTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGAACAAAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGCTTCtttt  <  2:988588/141‑1 (MQ=255)

ACAGTTATTGCACCTGAACATAAGCATAAATACAAAGATATTGAAAACGGATTAAAAGGTGAAGAAAAAGTATTAATTGAACAAATGGCGCAACATTGCGAAGCTTTTAAAGCTAATTTTAAAGGTGCAGCTCAAGGAGATTGGGTTAAAAGTGCCATGTCTGAGATAGACAGCATTAAGGATGACCTGAAAAAAATTAATAGCTAAACTACATATCATACTACTAAATAAAGCGACCAATGTTCAGTATATTCACAACTGACACTAGGCCGCTTCTTTT  >  USA300TCH1516_ALE/473812‑474091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`