Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | USA300TCH1516_ALE | 211,512 | T→A | 5.2% | intergenic (+70/+258) | sfp → / ← yagU | 4'‑phosphopantetheinyl transferase sfp/Inner membrane protein YagU |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | USA300TCH1516_ALE | 211,512 | 0 | T | A | 5.2% | 397.5 / 9.5 | 126 | intergenic (+70/+258) | sfp/yagU | 4'‑phosphopantetheinyl transferase sfp/Inner membrane protein YagU |
Reads supporting (aligned to +/- strand): ref base T (61/57); new base A (3/4); total (65/61) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.13e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01 |
Only 100 of 136 total aligned reads displayed.
GGTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTTGAG > USA300TCH1516_ALE/211373‑211651 | ggTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttt > 1:491675/1‑141 (MQ=255) gTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGtttt < 3:421653/141‑1 (MQ=255) tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt > 3:384657/1‑141 (MQ=255) tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt > 2:12389/1‑141 (MQ=255) tATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGttttt < 1:995148/141‑1 (MQ=255) aTGTTTCTTAGGACAGGCACCCTGGGTTTATAAAAAAGTGTCCGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGtttttt > 1:253561/1‑141 (MQ=255) gTTTCTTAGGACATGCACACTGGGGTCATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTa < 4:211775/141‑1 (MQ=255) tCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTAttt > 2:290173/1‑140 (MQ=255) cTTAAGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGGCGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTgg < 3:329954/141‑1 (MQ=255) ttAGGACAGGCACCCTGTGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATGGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTCCTGTTAGATGTTTTTTTATTGGa < 4:15436/141‑1 (MQ=255) ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa < 3:247768/141‑1 (MQ=255) ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa < 1:167336/141‑1 (MQ=255) ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa < 1:504852/141‑1 (MQ=255) ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGa < 4:334078/141‑1 (MQ=255) ttAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGACTAAATGTTTACTGTTAGATGTTTTTTTATTGGa > 1:1005696/1‑141 (MQ=255) tAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGaa < 1:906213/141‑1 (MQ=255) aGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTGGaaa > 2:977820/1‑141 (MQ=255) ggACAGGCAACCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGaaaa < 4:247017/141‑1 (MQ=255) gaGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTATTTTTATTGGAAAAATg > 4:349660/2‑141 (MQ=255) cAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATg < 1:677946/141‑1 (MQ=255) ggCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGaa > 1:445470/1‑141 (MQ=255) gCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGaaa > 1:992495/1‑141 (MQ=255) tGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGaa > 1:580792/1‑141 (MQ=255) tGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGaa < 3:227685/141‑1 (MQ=255) gggTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATa < 1:290132/141‑1 (MQ=255) ttATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATacac > 4:258778/1‑141 (MQ=255) tataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACg > 3:170649/1‑141 (MQ=255) ataAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGa < 3:70226/141‑1 (MQ=255) taAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGAt > 2:889478/1‑141 (MQ=255) aaaaaaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATg > 4:297488/1‑141 (MQ=255) aaaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGAGCTCGTAGTTTAAACGATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGca < 1:19467/141‑1 (MQ=255) aaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGcat < 3:5027/141‑1 (MQ=255) aaGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTACTGGAAAAATGAAAGGTGAATACACGATGGcat < 4:36086/141‑1 (MQ=255) aGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGcatc > 1:694643/1‑141 (MQ=255) gtCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCAtt > 3:378647/1‑141 (MQ=255) tCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTc > 1:160454/1‑141 (MQ=255) cTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCa > 2:665581/1‑141 (MQ=255) tATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCaaaa < 4:51388/141‑1 (MQ=255) tttCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATc < 3:302401/141‑1 (MQ=255) tCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAATCTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAt > 2:635252/1‑141 (MQ=255) tCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAt < 2:34476/141‑1 (MQ=255) gTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTGGATATATGAAAGGTGAATACACGAAGGCATCATTCAAAATTATgtc > 2:29732/1‑139 (MQ=255) gTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAAGGAAAGGTGAATACACGATGGCATCATTCAAAATCATGAc > 2:4810/1‑141 (MQ=255) ttGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACgaga > 1:114237/1‑141 (MQ=255) ttGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACgaga > 2:239549/1‑141 (MQ=255) tGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTATTTTTATTTGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGAt < 3:349660/141‑1 (MQ=255) gAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGAtt > 1:968945/1‑141 (MQ=255) gtTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagt < 3:88200/141‑1 (MQ=255) ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta < 1:223769/141‑1 (MQ=255) ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta < 1:309267/141‑1 (MQ=255) ttAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTATAAAAATAGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAaagta < 2:965626/141‑1 (MQ=255) atGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGa > 4:304075/1‑141 (MQ=255) cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg > 3:229236/1‑141 (MQ=255) cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg > 1:816030/1‑141 (MQ=255) cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg > 1:425209/1‑141 (MQ=255) cGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGATTACTGGTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAg > 1:170285/1‑141 (MQ=255) gTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTTTTTTTTTTATGAAAGGTGAATACACGATGGCATCATTCAAAATAATGAGGAGCTTAAAGTAAGTGATAAgg > 4:250753/1‑141 (MQ=255) tCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATCAAAGTAAGTGATAAGGt > 3:24160/1‑141 (MQ=255) cttaCAGTATCATGGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATTTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTa < 1:61687/138‑1 (MQ=255) cTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTa > 2:788859/1‑141 (MQ=255) gACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaa > 1:1080643/1‑141 (MQ=255) aCAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaaa < 2:107404/141‑1 (MQ=255) cAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTaaaaa < 1:363270/141‑1 (MQ=255) ttGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGa > 4:180234/1‑141 (MQ=255) ttGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGa < 1:931249/141‑1 (MQ=255) gcgcAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGc > 4:167485/1‑141 (MQ=255) gTGATTTTAAACTATACTGCCTCAATGTTTTCTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCAGGACGAGATTAAAGTAAGTGTTAAGGTAAAAATATACTGAGCAatat < 3:379475/141‑1 (MQ=255) gTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAatat < 1:600461/141‑1 (MQ=255) tGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATAtt < 2:9040/141‑1 (MQ=255) ttAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTGTTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAAt > 1:894670/1‑141 (MQ=255) ttAAACTATACTGCATAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGAATCATTCAAAAACATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATAATGgaaa > 2:526498/1‑140 (MQ=255) tAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAAtt < 3:418046/141‑1 (MQ=255) aaCTATACTGCCTAAATGTTTACTGTTAGATG‑TTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTAcc < 4:161029/141‑1 (MQ=255) tttttcTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa < 2:40122/136‑1 (MQ=255) aCTGGCTAAATATTTACTGTTAGATGTTTTTATATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa < 2:178701/141‑1 (MQ=255) aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa < 2:142109/141‑1 (MQ=255) aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGaaaa < 2:375055/141‑1 (MQ=255) aCTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGAGTAAAGTAAGGGATAAGGTAAAAATATACTGAGCAATAATGAAATTACCGaaaa > 3:180073/1‑141 (MQ=255) cTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaat < 2:939609/141‑1 (MQ=255) tGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaata < 3:219858/141‑1 (MQ=255) gCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaataa < 2:1010476/141‑1 (MQ=255) ccTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAaataat > 1:295492/1‑141 (MQ=255) aaaTGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGt > 3:283085/1‑141 (MQ=255) aaTGTTTTCTGTAAGAAGATTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACAGAGAAATAAACAAAAAAACAAATATAATAGtt < 2:766875/141‑1 (MQ=255) tGTTTACTGTTAGATGTTTTTTTTTTTTTTTTTTTAAAGGAGAATACACGCTGGCTTTATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTAAGCAATATTGAAATTACCGATTTTATTAGtttt > 2:703580/1‑140 (MQ=255) tGTTTACTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAataatatcttg < 2:696612/141‑6 (MQ=255) tGTTCACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGGAGACACGATGGCATCATTCAAAAGCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGATCAATATTGAAATTACCGAAAATAATAGTTTg > 4:438554/1‑141 (MQ=255) gTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGGGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGt > 3:154542/1‑141 (MQ=255) tttACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGtt > 1:939728/1‑141 (MQ=255) ttACTGTTAGATGTTTTTTTTTTTTTTTTATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATGAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTa > 2:309878/1‑141 (MQ=255) tACTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACATGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTac < 1:415987/141‑1 (MQ=255) aCTGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTacg > 1:352992/1‑141 (MQ=255) cTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacga < 1:711128/141‑1 (MQ=255) tGTTAGATGTATTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAATGTAAGTGATAAGGTGAAAATATACTGAGCAATATTGAAATTGCCAAAAATAATATCTTGTTacgac > 1:485488/1‑141 (MQ=255) gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATCTACTGCGCAATAGTGAAATTACCGAAAATAATAGTTTGTTacgacg > 2:555386/1‑141 (MQ=255) gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacgacg < 1:75742/141‑1 (MQ=255) gTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGAAAAAAAAAAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTacgacg < 2:445470/141‑1 (MQ=255) aTGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCAttt > 1:682973/1‑141 (MQ=255) aTGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCAttt > 2:875502/1‑141 (MQ=255) tGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTTg < 4:261808/141‑1 (MQ=255) | GGTATGTTTCTTAGGACAGGCACCCTGGGGTTATAAAAAAGTGTCTGTATTTCAGTTATTGAGTAGTTAATATGACGTCTGACAGTATCATTGCGCAGTGATTTTAAACTATACTGCCTAAATGTTTACTGTTAGATGTTTTTTTATTGGAAAAATGAAAGGTGAATACACGATGGCATCATTCAAAATCATGACGAGATTAAAGTAAGTGATAAGGTAAAAATATACTGAGCAATATTGAAATTACCGAAAATAATAGTTTGTTACGACGCATTTGAG > USA300TCH1516_ALE/211373‑211651 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |