Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,833,850 A→T 6.0% L179L (CTT→CTA hisI ← Phosphoribosyl‑AMP cyclohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,833,8500AT 6.0% 474.9 / 16.3 150L179L (CTT→CTAhisIPhosphoribosyl‑AMP cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (73/67);  new base T (5/4);  total (79/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

Only 100 of 152 total aligned reads displayed.

TATAAGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCTATACCTTC  >  USA300TCH1516_ALE/2833710‑2833990
                                                                                                                                            |                                                                                                                                            
tataAGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCa                                                                                                                                              <  1:1014655/141‑1 (MQ=255)
  taAGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAg                                                                                                                                            >  1:692221/1‑141 (MQ=255)
   aaGCCTTGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAACGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAgc                                                                                                                                           <  1:591332/141‑1 (MQ=255)
   aaGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAgc                                                                                                                                           >  3:210162/1‑141 (MQ=255)
    aGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAgcg                                                                                                                                          >  1:1046255/1‑141 (MQ=255)
    aGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAgcg                                                                                                                                          >  1:504170/1‑141 (MQ=255)
     gCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAgcgc                                                                                                                                         >  3:395641/1‑141 (MQ=255)
     gCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAgcgc                                                                                                                                         <  1:204702/141‑1 (MQ=255)
      ccATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgca                                                                                                                                        <  4:18244/141‑1 (MQ=255)
        aTGATCCTTTTCCTTATATCTTAGTCCATCCTTGCTCTACCACTGTTCGATACTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatg                                                                                                                                      <  4:29672/141‑1 (MQ=255)
         tGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatgc                                                                                                                                     >  4:78365/1‑141 (MQ=255)
         tGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatgc                                                                                                                                     >  2:888050/1‑141 (MQ=255)
         tGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatgc                                                                                                                                     <  1:480871/141‑1 (MQ=255)
          gATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTGTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatgca                                                                                                                                    >  2:580428/1‑141 (MQ=255)
           aTCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCgcatgcat                                                                                                                                   <  2:1043241/141‑1 (MQ=255)
            tCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATc                                                                                                                                  <  1:1047948/141‑1 (MQ=255)
            tCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATc                                                                                                                                  <  4:29284/141‑1 (MQ=255)
             ccTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGGCGTTCTCCTTTAGATTGATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCa                                                                                                                                 >  3:44413/1‑141 (MQ=255)
              cTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCaa                                                                                                                                >  1:35842/1‑141 (MQ=255)
              cTTTTCCTTATAAATAAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCaa                                                                                                                                >  3:451164/1‑141 (MQ=255)
               ttttCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAg                                                                                                                               <  3:5784/141‑1 (MQ=255)
                tttCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGa                                                                                                                              <  2:434146/141‑1 (MQ=255)
                      tataTCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACaaata                                                                                                                        >  3:295986/1‑141 (MQ=255)
                      tataTCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCAAAAAAAAAGAAAAATCGACGCCAAGCGCATGCATCAAGACaaata                                                                                                                        <  2:1080171/141‑1 (MQ=255)
                        tatCTTAGTCCATACTTGATTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGAAAAAAAAAAAAAAAAATCGACGCCAAGCGCATGCATCAAGACaaataaa                                                                                                                      <  2:471916/141‑1 (MQ=255)
                          tCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATg                                                                                                                    >  2:494369/1‑141 (MQ=255)
                            ttAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGAt                                                                                                                  <  1:711004/141‑1 (MQ=255)
                              aGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATaa                                                                                                                >  2:121279/1‑141 (MQ=255)
                              aGTCCATACTTGCTTTACAGCTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATaa                                                                                                                <  3:229991/141‑1 (MQ=255)
                               gTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATaaa                                                                                                               <  1:912550/141‑1 (MQ=255)
                                tCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAg                                                                                                              <  1:699143/141‑1 (MQ=255)
                                    tACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCa                                                                                                          <  4:457102/141‑1 (MQ=255)
                                      cTTGCTTTACCACTGTTCGATATTTTGTCGTTCACATTATAAGTTCTTGCTAATATGATGTCTTTGTCCTAATCCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATc                                                                                                        <  1:757357/141‑1 (MQ=255)
                                      cTTGCTTTACCACTGTTCGATATTTCGGCGTTCACCTGTAAAGATATCGCGCTTATGATGTCTACGCGCTAATTCCCCCTCAATTTCTGAAAAATCGACGCCAAACGCATGCATCAAGACACATAAATGATaaaatgcagg                                                                                                        >  2:1030527/1‑134 (MQ=255)
                                         gCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGc                                                                                                     >  3:249810/1‑141 (MQ=255)
                                            ttACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTAc                                                                                                  >  2:208116/1‑141 (MQ=255)
                                              aCCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACtt                                                                                                <  1:159963/141‑1 (MQ=255)
                                              aCCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACtt                                                                                                >  1:521389/1‑141 (MQ=255)
                                               ccACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTc                                                                                               >  4:368953/1‑141 (MQ=255)
                                               ccACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTc                                                                                               >  4:368963/1‑141 (MQ=255)
                                                cACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTcc                                                                                              >  2:586879/1‑140 (MQ=255)
                                                cACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCa                                                                                              <  1:1003320/141‑1 (MQ=255)
                                                cACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCa                                                                                              >  1:591316/1‑141 (MQ=255)
                                                       cGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaa                                                                                       <  3:368953/141‑1 (MQ=255)
                                                       cGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaa                                                                                       <  3:368963/141‑1 (MQ=255)
                                                       cGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaa                                                                                       >  1:449912/1‑141 (MQ=255)
                                                       cGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaa                                                                                       >  2:451826/1‑141 (MQ=255)
                                                       cGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaa                                                                                       >  2:137107/1‑141 (MQ=255)
                                                        gATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaaa                                                                                      <  1:905145/141‑1 (MQ=255)
                                                        gATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACaaa                                                                                      <  2:412434/141‑1 (MQ=255)
                                                           atTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGc                                                                                   <  1:472258/141‑1 (MQ=255)
                                                                gTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCttttt                                                                              >  1:984277/1‑141 (MQ=255)
                                                                      cACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCAc                                                                        >  1:403776/1‑141 (MQ=255)
                                                                        ccTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCt                                                                      <  3:110413/141‑1 (MQ=255)
                                                                            tAAAGTTATTGCGCTTATGAGGTCTACGCGCTAATTCCGCCTCAATGTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTaa                                                                  <  1:1032607/141‑1 (MQ=255)
                                                                              aaGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAAtt                                                                <  4:320045/141‑1 (MQ=255)
                                                                               tgTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTg                                                               <  2:466122/140‑1 (MQ=255)
                                                                               aGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTg                                                               <  1:869868/141‑1 (MQ=255)
                                                                               aGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTg                                                               <  1:45669/141‑1 (MQ=255)
                                                                                    ttGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCa                                                          <  2:301460/141‑1 (MQ=255)
                                                                                       cgcTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAAtt                                                       >  2:72327/1‑141 (MQ=255)
                                                                                        gcTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCTAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTa                                                      >  2:415695/1‑141 (MQ=255)
                                                                                          ttATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGAATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTatg                                                    >  3:314517/1‑139 (MQ=255)
                                                                                                tgTCTACGCGCGAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATTCATCTAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTGAATTACGACTTCa                                              >  2:918537/1‑141 (MQ=255)
                                                                                                 gTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCaa                                             <  1:730402/141‑1 (MQ=255)
                                                                                                 gTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCaa                                             >  2:959272/1‑141 (MQ=255)
                                                                                                    tACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAg                                          <  3:320251/141‑1 (MQ=255)
                                                                                                     aCGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGc                                         <  2:1050545/141‑1 (MQ=255)
                                                                                                      cgcgCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGct                                        >  2:931968/1‑141 (MQ=255)
                                                                                                         gcTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTCATTGCTTCAATTACGACTTCAAAAGcttct                                     >  3:318734/1‑141 (MQ=255)
                                                                                                         gcTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGcttct                                     >  2:296822/1‑141 (MQ=255)
                                                                                                          cTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGcttctt                                    >  1:765447/1‑141 (MQ=255)
                                                                                                                ccGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGt                              >  1:229263/1‑141 (MQ=255)
                                                                                                                ccGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGt                              >  2:62935/1‑141 (MQ=255)
                                                                                                                  gCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGCCAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAt                            >  1:933735/1‑141 (MQ=255)
                                                                                                                  gCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTTTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAt                            >  2:187006/1‑141 (MQ=255)
                                                                                                                  gCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAt                            >  3:247346/1‑141 (MQ=255)
                                                                                                                  gCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAt                            >  4:193695/1‑141 (MQ=255)
                                                                                                                  gCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAt                            >  2:922112/1‑141 (MQ=255)
                                                                                                                      cAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAttttt                        >  3:431889/1‑141 (MQ=255)
                                                                                                                       aaTTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAtttttt                       >  3:278728/1‑141 (MQ=255)
                                                                                                                        aTTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAttttttg                      <  2:968337/141‑2 (MQ=255)
                                                                                                                         tttCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGANTTCAAAAGCTTCNTCACCGTATTTTTTTg                     <  1:912938/141‑1 (MQ=255)
                                                                                                                         tttCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTg                     <  4:117878/141‑1 (MQ=255)
                                                                                                                          ttCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTAttttttttt                    >  4:193865/1‑139 (MQ=255)
                                                                                                                          ttCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGt                    <  2:142493/141‑1 (MQ=255)
                                                                                                                            cTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTaa                  >  1:82095/1‑141 (MQ=255)
                                                                                                                              gAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATc                <  4:325750/141‑1 (MQ=255)
                                                                                                                              gAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATc                <  2:549195/141‑1 (MQ=255)
                                                                                                                               aaaaaTCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCCCCTTTAAGTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCt               <  1:1030527/141‑1 (MQ=255)
                                                                                                                               aaaaaTCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCt               >  2:71566/1‑141 (MQ=255)
                                                                                                                                aaaaTCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTGCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCtt              <  1:157990/141‑1 (MQ=255)
                                                                                                                                   aTCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTc           >  2:193355/1‑141 (MQ=255)
                                                                                                                                    tCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCt          >  2:61978/1‑141 (MQ=255)
                                                                                                                                     cGACGCCAAGCGCACGCATCAAGACAAATAAACAATAAAGTTCAACTGCTACTACACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCtc         >  2:763467/1‑140 (MQ=255)
                                                                                                                                      gACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCtat        >  2:444602/1‑141 (MQ=255)
                                                                                                                                      gACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCtat        <  2:145372/141‑1 (MQ=255)
                                                                                                                                      gACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCtat        <  3:410565/141‑1 (MQ=255)
                                                                                                                                       aCGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTTTTTTTTTTTTTTGCTTCAATTACGAGTTCAAAAGTTTCTTCACCGTATTTTTTTGTAATCTTTTCtatt       >  4:425488/1‑140 (MQ=255)
                                                                                                                                        cGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCTATAc      >  2:291113/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TATAAGCCATGATCCTTTTCCTTATATCTTAGTCCATACTTGCTTTACCACTGTTCGATATTTTGTCGTTCACCTTTAAAGTTATTGCGCTTATGATGTCTACGCGCTAATTCCGCCTCAATTTCTGAAAAATCGACGCCAAGCGCATGCATCAAGACAAATAAATGATAAAGTTCATCTGCTACTTCACTTACAAATGCTTTTTTGTCACCTTTAATTGCTTCAATTACGACTTCAAAAGCTTCTTCACCGTATTTTTTTGTAATCTTTTCTATACCTTC  >  USA300TCH1516_ALE/2833710‑2833990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: