BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,831,977	G→T	8.6%	Q230K (CAA→AAA)	lipA_2 ←	Lipase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,831,977	0	G	T	8.6%	477.6 / 6.5	156	Q230K (CAA→AAA)	lipA_2	Lipase 1
Reads supporting (aligned to +/- strand): ref base G (53/86); new base T (15/2); total (68/88)
Fisher's exact test for biased strand distribution p-value = 1.12e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

CCCTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACTTATTTAATG  >  USA300TCH1516_ALE/2831837‑2832117

cccTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTg                                                                                                                                              >  1:1028775/1‑141 (MQ=255)

cccTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTg                                                                                                                                              >  1:428779/1‑141 (MQ=255)

cccTTGGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTg                                                                                                                                              >  2:813660/1‑141 (MQ=255)

  cTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAg                                                                                                                                            >  1:655449/1‑141 (MQ=255)

   tttgtttgGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAgg                                                                                                                                           >  1:470916/1‑141 (MQ=255)

     tgtttgGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGtt                                                                                                                                         <  3:175079/141‑1 (MQ=255)

      gtttgGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGttt                                                                                                                                        <  2:412206/141‑1 (MQ=255)

        ttgGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCa                                                                                                                                      <  3:110383/141‑1 (MQ=255)

          ggTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCAct                                                                                                                                    >  2:977417/1‑141 (MQ=255)

          ggTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCAct                                                                                                                                    >  4:117047/1‑141 (MQ=255)

          ggTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCAct                                                                                                                                    <  2:133993/141‑1 (MQ=255)

           gaaaCGTTGCTACTGCAATACTTTTTATTAATTTTAATCCATTTTTCTTTTCATTGTCATTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAATTTTGATAACAATAGATTTTTTTTGTTTTTTTGAGGTTTCTctt                                                                                                                                   >  4:179106/3‑141 (MQ=255)

           gTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTAGGTTTCActt                                                                                                                                   >  2:672577/1‑141 (MQ=255)

           gTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCActt                                                                                                                                   <  3:413645/141‑1 (MQ=255)

           gTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGATGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTTATGTTTCActt                                                                                                                                   >  4:193519/1‑141 (MQ=255)

            tAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTTTCATTGTCCTTTGTTGTTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCActtc                                                                                                                                  <  1:491105/141‑1 (MQ=255)

                 ttGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGtt                                                                                                                             <  1:517710/141‑1 (MQ=255)

                    cTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTGGGTTCTACTGGTGAATTTTGATTTTTTTTTAAATCTGAAACTGATAGTGCATTGTCATCTTTTTGATGTTTCActtcttcatctt                                                                                                                          >  2:590749/1‑135 (MQ=255)

                       cTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGt                                                                                                                       >  1:307673/1‑141 (MQ=255)

                        tGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGtt                                                                                                                      <  2:398629/141‑1 (MQ=255)

                        tGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGtt                                                                                                                      <  1:532691/141‑1 (MQ=255)

                        tGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGtt                                                                                                                      <  1:902105/141‑1 (MQ=255)

                          cACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTaa                                                                                                                    <  2:19408/141‑1 (MQ=255)

                          cACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTaa                                                                                                                    <  1:15588/141‑1 (MQ=255)

                              actTTTTAATAAATTTAATCCATTTTTTTTTTTTTTTTTTTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTTATTGTGTTTTTTCTTTTTTTTTAGGTTTTTTTTTTTTTTCTTTGtttatttt                                                                                                                >  2:111334/1‑135 (MQ=255)

                               ctTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTg                                                                                                               <  3:199767/141‑1 (MQ=255)

                                 ttttAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGta                                                                                                             <  2:455752/141‑1 (MQ=255)

                                   ttAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTTATTGTTAAAATTGtagt                                                                                                           >  3:32090/1‑141 (MQ=255)

                                    taataaATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGATGATTATTGTTTCTTTTTTAAACCTGATTCTTTTAGTGTTTTGTCTTCGTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGtagtt                                                                                                          >  1:758440/1‑141 (MQ=255)

                                     aataaATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTATTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGtagtta                                                                                                         <  4:20087/141‑1 (MQ=255)

                                      ataaATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGTGGTTTCACTTCTTGTTCATTGTTAAAATTGtagttag                                                                                                        >  1:200836/1‑141 (MQ=255)

                                        aaaTTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTa                                                                                                      >  1:534262/1‑141 (MQ=255)

                                         aaTTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTATTTAGTaa                                                                                                     >  2:69544/1‑141 (MQ=255)

                                          aTTTAATCCATTTTTCTTGTCATTGTCTTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTGGTTAGTAAt                                                                                                    <  3:158131/141‑1 (MQ=255)

                                           tttAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAAtt                                                                                                   >  1:111499/1‑141 (MQ=255)

                                               aTCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTATTCTTTTTTAGTTTTTACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTAt                                                                                               >  1:805031/1‑141 (MQ=255)

                                                 ccATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCa                                                                                             <  3:67096/141‑1 (MQ=255)

                                                 ccATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCa                                                                                             >  3:265767/1‑141 (MQ=255)

                                                   aTTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCAtt                                                                                           <  2:638132/141‑1 (MQ=255)

                                                    tttttCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTc                                                                                          >  1:172380/1‑141 (MQ=255)

                                                       ttCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCt                                                                                       <  4:238643/141‑1 (MQ=255)

                                                        tCTTGTCATTGTCCTTTGTTGTTTCTACTGGTGAATTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTATTTTCGTCTTTTTTAGGTTTCACTTCTTGTTCATTGTTAAAAATGGAGTTAGTAATTTTAGCATTCGCtt                                                                                      <  2:312352/141‑1 (MQ=255)

                                                        tCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCtt                                                                                      <  2:705699/141‑1 (MQ=255)

                                                          ttGTCATTGTCTTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTAGGTTTCACTTCTTGTTCATTGTTAAAATTGTGGTTAGTAATTTTATCATTCGCTTgt                                                                                    >  2:409283/1‑140 (MQ=255)

                                                          ttGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGa                                                                                    <  2:228994/141‑1 (MQ=255)

                                                           tGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGat                                                                                   <  4:336876/141‑1 (MQ=255)

                                                            gTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGatt                                                                                  >  1:755242/1‑141 (MQ=255)

                                                              taTTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGattat                                                                                <  2:520991/140‑1 (MQ=255)

                                                                ttGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTAttt                                                                              >  1:378080/1‑141 (MQ=255)

                                                                      tttGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTtgttgt                                                                        >  3:390786/1‑141 (MQ=255)

                                                                         gttgGTTCTACTGTTGATTTGTGATTGTTGTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGGTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGt                                                                     <  2:539914/141‑1 (MQ=255)

                                                                         gttgGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGt                                                                     <  3:422283/141‑1 (MQ=255)

                                                                          ttgGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGTTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGtt                                                                    <  1:1027451/141‑1 (MQ=255)

                                                                            ggTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGt                                                                  >  3:248752/1‑141 (MQ=255)

                                                                            ggTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGt                                                                  <  2:753610/141‑1 (MQ=255)

                                                                              ttCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTAc                                                                <  2:985471/141‑1 (MQ=255)

                                                                              ttCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTAc                                                                <  2:777836/141‑1 (MQ=255)

                                                                                cTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACtt                                                              <  1:813660/141‑1 (MQ=255)

                                                                                cTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACtt                                                              <  2:428779/141‑1 (MQ=255)

                                                                                    tGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTtctc                                                          <  2:782371/141‑1 (MQ=255)

                                                                                    tGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGCTGTACTTtctc                                                          <  3:278833/141‑1 (MQ=255)

                                                                                      gTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTa                                                        <  2:95868/141‑1 (MQ=255)

                                                                                       tGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAt                                                       >  1:353261/1‑141 (MQ=255)

                                                                                       tGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAt                                                       <  2:74944/141‑1 (MQ=255)

                                                                                       tGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAt                                                       >  1:500963/1‑141 (MQ=255)

                                                                                        gATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAtt                                                      <  4:302644/141‑1 (MQ=255)

                                                                                         aTTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCGTGTGCATTGTTAAAATTGTAGTTAGTAAGTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTAttt                                                     >  1:648056/1‑141 (MQ=255)

                                                                                           ttttGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCt                                                   >  1:524156/1‑141 (MQ=255)

                                                                                             ttGATTGTTTTTTAATTCTGAAACTGATAGTGTTTTTTCTTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGt                                                 <  3:89324/141‑1 (MQ=255)

                                                                                             ttGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCTTCTTTTTGAGTGTTCACTTCTTGTTCATTTTTAAAATTGTAGTTAGTAATTTTATTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTCTTTCTCTATTTTTGt                                                 >  4:151187/1‑141 (MQ=255)

                                                                                               gATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGtct                                               >  1:761609/1‑141 (MQ=255)

                                                                                               gATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGtct                                               >  1:744774/1‑141 (MQ=255)

                                                                                                  tGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCTTTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCAt                                            <  4:30297/141‑1 (MQ=255)

                                                                                                   gTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCAtt                                           <  1:977417/141‑1 (MQ=255)

                                                                                                     tttttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTaa                                         <  3:179106/141‑1 (MQ=255)

                                                                                                     tttttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTaa                                         <  3:44915/141‑1 (MQ=255)

                                                                                                      ttttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAAc                                        <  2:1026837/141‑1 (MQ=255)

                                                                                                       tttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACa                                       <  3:63811/141‑1 (MQ=255)

                                                                                                        ttAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACaa                                      <  1:159759/141‑1 (MQ=255)

                                                                                                         tAAATCTGAAACTGATAGTGTTTTTTTTTTTTTTTTTTGTTTCACTTCTTGTTCATTGTTAAATTTGTAGTTAGTAATTTTATCATTCGTTTTTTTATTTTGTTGTGGTTGTTTTTTTTTTTTTTTTTTTTCATTattatt                                     >  2:560664/1‑136 (MQ=255)

                                                                                                          gaaTCTGAACCTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAGTTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAAtt                                    <  2:911553/140‑1 (MQ=255)

                                                                                                          aaaTCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAAtt                                    >  2:848848/1‑141 (MQ=255)

                                                                                                           aaTCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTGGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAAttt                                   >  1:447116/1‑141 (MQ=255)

                                                                                                             tCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTtct                                 <  4:379187/141‑1 (MQ=255)

                                                                                                              cTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTtctc                                <  2:499062/141‑1 (MQ=255)

                                                                                                                gAAACTGATAGTGTTTTTTTTTTTTTTTTTGGTTTCAATTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTTATTATTTTTTTTTTATTGTACTTTCTCTATTTCTGTTTCTTTAACTTTTTCTCGt                              >  2:446088/1‑141 (MQ=255)

                                                                                                                gAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGt                              <  3:214496/141‑1 (MQ=255)

                                                                                                                gAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGt                              <  2:307673/141‑1 (MQ=255)

                                                                                                                 aaaCTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGtt                             <  2:183984/141‑1 (MQ=255)

                                                                                                                  aaCTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTg                            <  4:253989/141‑1 (MQ=255)

                                                                                                                    cTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGAc                          <  4:398510/141‑1 (MQ=255)

                                                                                                                     tGATAGTGTTTTTTCGTCTTTTTGTGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTGATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACt                         <  4:393854/141‑1 (MQ=255)

                                                                                                                     tGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACt                         <  2:862027/141‑1 (MQ=255)

                                                                                                                        tAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGt                      <  2:515230/141‑1 (MQ=255)

                                                                                                                        tAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGt                      <  1:709268/141‑1 (MQ=255)

                                                                                                                               ttttCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTTACAATTTCTCGTTGACTTGTTTGATCt               >  3:63021/1‑141 (MQ=255)

                                                                                                                                 ttCGTCTTTTTTATGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTaa             <  1:696576/141‑1 (MQ=255)

                                                                                                                                  tCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTACCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAAc            >  3:344796/1‑141 (MQ=255)

                                                                                                                                    gTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACtt          <  1:751279/141‑1 (MQ=255)

                                                                                                                                       tttttGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACTTAtt       <  4:151349/141‑1 (MQ=255)

CCCTTTGTTTGGTAACGTTGCTACTGCACTACTTTTTAATAAATTTAATCCATTTTTCTTGTCATTGTCCTTTGTTGGTTCTACTGGTGATTTTTGATTGTTTTTTAAATCTGAAACTGATAGTGTTTTTTCGTCTTTTTGAGGTTTCACTTCTTGTTCATTGTTAAAATTGTAGTTAGTAATTTTATCATTCGCTTGATTATTTTGTTGTGGTTGTACTTTCTCTATTTCTGTCTCATTAACAATTTCTCGTTGACTTGTTTGATCTAACTTATTTAATG  >  USA300TCH1516_ALE/2831837‑2832117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`