BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,654,178	C→T	5.4%	T147T (ACC→ACT)	USA300HOU_RS13650 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,654,178	0	C	T	5.4%	436.0 / 7.8	140	T147T (ACC→ACT)	USA300HOU_RS13650	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (67/65); new base T (3/5); total (70/70)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 143 total aligned reads displayed.

TGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATT  >  USA300TCH1516_ALE/2654039‑2654316

tGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTAccc                                                                                                                                           <  2:350235/141‑1 (MQ=255)

tGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTAccc                                                                                                                                           <  3:231124/141‑1 (MQ=255)

 gACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTAcccc                                                                                                                                          >  4:154396/1‑141 (MQ=255)

  ccTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGGTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCa                                                                                                                                         <  2:1011840/140‑1 (MQ=255)

   cTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAt                                                                                                                                        >  1:298549/1‑141 (MQ=255)

     gCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAttt                                                                                                                                      >  1:401847/1‑141 (MQ=255)

      cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                                     <  3:136600/141‑1 (MQ=255)

      cAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAtttt                                                                                                                                     >  2:116568/1‑141 (MQ=255)

       aGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCAttttt                                                                                                                                    >  2:736614/1‑141 (MQ=255)

        ggtggtGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTa                                                                                                                                   >  1:886586/1‑141 (MQ=255)

            gtgCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTg                                                                                                                               >  3:186491/1‑141 (MQ=255)

             tgCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGc                                                                                                                              >  2:698159/1‑141 (MQ=255)

               cGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGa                                                                                                                            >  1:622767/1‑141 (MQ=255)

                    aCCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTc                                                                                                                       >  2:282334/1‑141 (MQ=255)

                       aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                                    >  1:84525/1‑141 (MQ=255)

                       aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                                    <  3:400965/141‑1 (MQ=255)

                       aTTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCAtt                                                                                                                    <  2:533735/141‑1 (MQ=255)

                        ttCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTa                                                                                                                   >  3:4162/1‑141 (MQ=255)

                         tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCCACCCTGTTTCTACAATTCGATCGTTTCATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                                  <  1:488338/141‑1 (MQ=255)

                         tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                                  <  1:991300/141‑1 (MQ=255)

                         tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                                  >  3:148367/1‑141 (MQ=255)

                         tcatcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTAt                                                                                                                  <  1:947317/141‑1 (MQ=255)

                          cgtcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATg                                                                                                                 >  3:398535/3‑141 (MQ=255)

                            tcaAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGAt                                                                                                               <  3:173297/141‑1 (MQ=255)

                              aaaGTTTCAACTTACCGTATTGGCTGAGCGCTCTCATATTAGTCGTCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTg                                                                                                             <  2:126801/141‑1 (MQ=255)

                                aGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCg                                                                                                           >  1:625725/1‑141 (MQ=255)

                                    tCAACTTGCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTct                                                                                                       >  1:861031/1‑141 (MQ=255)

                                    tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                             >  2:822697/1‑135 (MQ=255)

                                    tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                             <  1:822589/135‑1 (MQ=255)

                                    tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTg                                                                                                             >  2:822589/1‑135 (MQ=255)

                                    tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCCTTATGATTGCGGTct                                                                                                       >  4:81240/1‑141 (MQ=255)

                                    tCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGCct                                                                                                       >  3:258562/1‑141 (MQ=255)

                                     cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTcta                                                                                                      >  2:768659/1‑141 (MQ=255)

                                     cAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGActa                                                                                                      >  1:539437/1‑141 (MQ=255)

                                       aCTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTctact                                                                                                    <  3:178707/141‑1 (MQ=255)

                                         ttACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAt                                                                                                  >  1:444396/1‑141 (MQ=255)

                                           aCCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTAttt                                                                                                >  1:775293/1‑141 (MQ=255)

                                                aTTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaa                                                                                           <  1:990426/141‑1 (MQ=255)

                                                 ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTCATTGCGATTGTCATTATGATTGCGGGCTACTATTTCacaac                                                                                          >  2:106226/1‑141 (MQ=255)

                                                 ttGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCacaac                                                                                          >  2:325646/1‑141 (MQ=255)

                                                        aGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCAt                                                                                   >  2:969614/1‑141 (MQ=255)

                                                             aCTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGa                                                                              >  2:76066/1‑141 (MQ=255)

                                                               tCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGAtt                                                                            >  3:77484/1‑141 (MQ=255)

                                                                  tatTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                         <  2:139240/141‑1 (MQ=255)

                                                                  tatTAGTCGCCTTTATTTTAGCAACACTGTTTATAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTa                                                                         >  1:134888/1‑141 (MQ=255)

                                                                   atTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTAc                                                                        <  2:964018/141‑1 (MQ=255)

                                                                     tAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTg                                                                      <  2:1080244/141‑1 (MQ=255)

                                                                      aGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGc                                                                     <  1:201361/141‑1 (MQ=255)

                                                                        tCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCg                                                                   <  1:422127/141‑1 (MQ=255)

                                                                          gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                                 >  1:819666/1‑141 (MQ=255)

                                                                          gCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCt                                                                 <  1:733766/141‑1 (MQ=255)

                                                                           ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGTGGAGTTACTCCATTTTTAATTGCAATTGTCATTATGATTGCGGTCTACTATTTCACAACGAGTCATCTTGATTTTACTGCCGCta                                                                <  1:1046421/141‑1 (MQ=255)

                                                                           ccTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCtc                                                                <  1:1030610/141‑2 (MQ=255)

                                                                                aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATTATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                           <  3:214764/141‑1 (MQ=255)

                                                                                aTTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTaataat                                                           >  2:300783/1‑141 (MQ=255)

                                                                                 ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                          <  1:917231/141‑1 (MQ=255)

                                                                                 ttttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatg                                                          >  4:300818/1‑141 (MQ=255)

                                                                                   ttAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAatgat                                                        <  1:766676/141‑1 (MQ=255)

                                                                                     aGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGc                                                      <  1:385709/141‑1 (MQ=255)

                                                                                      gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                                     >  2:592010/1‑141 (MQ=255)

                                                                                      gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                                     >  1:707687/1‑141 (MQ=255)

                                                                                      gCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCt                                                     <  1:724916/141‑1 (MQ=255)

                                                                                       cAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTc                                                    <  2:724541/141‑1 (MQ=255)

                                                                                         acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                                  <  2:411678/141‑1 (MQ=255)

                                                                                         acacTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGACTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAg                                                  <  2:539437/141‑1 (MQ=255)

                                                                                            cTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGAtt                                               >  1:662063/1‑141 (MQ=255)

                                                                                              gTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCa                                             <  4:364490/141‑1 (MQ=255)

                                                                                               tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                            >  4:72772/1‑141 (MQ=255)

                                                                                               tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                            <  1:944274/141‑1 (MQ=255)

                                                                                               tttCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCAt                                            <  1:311521/141‑1 (MQ=255)

                                                                                                  cTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAg                                         <  3:71835/141‑1 (MQ=255)

                                                                                                   tAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGc                                        <  4:127933/141‑1 (MQ=255)

                                                                                                    aaaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTCCCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCa                                       <  1:942558/141‑1 (MQ=255)

                                                                                                     aaaTTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAg                                      >  3:345261/1‑141 (MQ=255)

                                                                                                         tCGCTCGTTTAATTTCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAAt                                  <  2:340212/141‑1 (MQ=255)

                                                                                                          cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                                 <  1:415019/141‑1 (MQ=255)

                                                                                                          cGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATc                                 >  2:451789/1‑141 (MQ=255)

                                                                                                             tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                              >  1:315445/1‑141 (MQ=255)

                                                                                                             tCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCAtt                              >  1:496211/1‑141 (MQ=255)

                                                                                                              cGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTa                             >  2:105480/1‑141 (MQ=255)

                                                                                                               gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                            <  2:1014765/141‑1 (MQ=255)

                                                                                                               gTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTAt                            <  1:53838/141‑1 (MQ=255)

                                                                                                                   aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTCCTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                        <  2:501313/141‑1 (MQ=255)

                                                                                                                   aaTTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCAcc                        <  1:619872/141‑1 (MQ=255)

                                                                                                                     ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg                      >  3:372882/1‑141 (MQ=255)

                                                                                                                     ttGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTg                      <  2:625967/141‑1 (MQ=255)

                                                                                                                      tGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATGATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTgg                     <  2:933884/141‑1 (MQ=255)

                                                                                                                        cTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAt                   <  1:785981/141‑1 (MQ=255)

                                                                                                                         tgtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtg                  >  4:154632/1‑141 (MQ=255)

                                                                                                                         tgtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtg                  >  3:106432/1‑141 (MQ=255)

                                                                                                                         tgtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGAtg                  >  2:162629/1‑141 (MQ=255)

                                                                                                                          gtgCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACTTGGAtgg                 >  4:316344/1‑141 (MQ=255)

                                                                                                                              ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt             <  1:282334/141‑1 (MQ=255)

                                                                                                                              ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt             <  1:251530/141‑1 (MQ=255)

                                                                                                                              ttGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATggtggt             <  2:586348/141‑1 (MQ=255)

                                                                                                                               tGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGtt            <  2:994056/141‑1 (MQ=255)

                                                                                                                               tGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGtt            <  4:381261/141‑1 (MQ=255)

                                                                                                                                  cGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGa         >  1:194854/1‑141 (MQ=255)

                                                                                                                                        tACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGAt   <  2:650460/141‑1 (MQ=255)

                                                                                                                                         aCCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGAtt  >  3:59097/1‑141 (MQ=255)

TGACTGCAGGTGGTGCGTCAACCATTCATCAAAGTTTCAACTTACCGTATTGGCTGAGCGCACTCATATTAGTCGCCTTTATTTTAGCAACACTGTTTCTAAAATTCGATCGTTTAATTGCTGTGCTTGGCGGTGTTACCCCATTTTTAATTGCGATTGTCATTATGATTGCGGTCTACTATTTCACAACAAGTCATCTTGATTTTACTGCCGCTAATAATGATGCTCAGATTCATAAGCAGAAATCATTATCACCTGGATGGTGGTTTGATGCGATT  >  USA300TCH1516_ALE/2654039‑2654316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`